- Research Article
- Open Access
Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells
© Jensen et al. 2016
Received: 1 September 2015
Accepted: 17 February 2016
Published: 22 March 2016
Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major cause of gastroenteritis in cattle and humans. Dendritic cells (DC) and macrophages (Mø) are major players in early immunity to Salmonella, and their response could influence the course of infection. Therefore, the global transcriptional response of bovine monocyte-derived DC and Mø to stimulation with live and inactivated S. Typhimurium was compared. Both cell types mount a major response 2 h post infection, with a core common response conserved across cell-type and stimuli. However, three of the most affected pathways; inflammatory response, regulation of transcription and regulation of programmed cell death, exhibited cell-type and stimuli-specific differences. The expression of a subset of genes associated with these pathways was investigated further. The inflammatory response was greater in Mø than DC, in the number of genes and the enhanced expression of common genes, e.g., interleukin (IL) 1B and IL6, while the opposite pattern was observed with interferon gamma. Furthermore, a large proportion of the investigated genes exhibited stimuli-specific differential expression, e.g., Mediterranean fever. Two-thirds of the investigated transcription factors were significantly differentially expressed in response to live and inactivated Salmonella. Therefore the transcriptional responses of bovine DC and Mø during early S. Typhimurium infection are similar but distinct, potentially due to the overall function of these cell-types. The differences in response of the host cell will influence down-stream events, thus impacting on the subsequent immune response generated during the course of the infection.
Salmonella enterica is one of the major causes of food-borne disease worldwide. Over 2500 serovars of S. enterica have been identified, which exhibit differences in host-specificity and disease outcome. S. enterica serovars Typhi (S. Typhi) and Dublin (S. Dublin) exhibit restricted host specificity, principally causing systemic disease in humans and cattle respectively. In contrast, S. enterica serovar Typhimurium (S. Typhimurium) infects a broad range of unrelated host species, including cattle and humans, causing gastroenteritis. S. Typhimurium rarely causes systemic disease, except in mice, where the disease mimics Typhoid fever in humans caused by S. Typhi . In cattle, S. Typhimurium infection most commonly causes clinical disease in calves between 2 and 6 weeks of age. Symptoms mirror those observed in humans and include diarrhoea, anorexia and pyrexia within 12–48 h of infection . Infected cattle can excrete 108 cfu Salmonella per gram of faeces and therefore are a major source of contamination and a potential risk to other cattle and humans.
S. Typhimurium is one of the major serovars causing disease in cattle in the US and UK [2, 3]. A large proportion of S. Typhimurium infections in the UK involve strain DT104, which contains a phage encoding for resistance to most antimicrobials [3, 4]. Therefore, alternative methods of control are needed, the development of which requires further understanding of the host-pathogen interactions occurring during infection. The only vaccine licenced in the UK against Salmonella infection in cattle consists of inactivated S. Dublin and S. Typhimurium. This vaccine does not induce sterile immunity but decreases the risk of disease and reduces shedding and is principally used during outbreaks .
Four hours after experimental oral challenge of calves, S. Typhimurium was found to have traversed the ileal epithelium and was detected within phagocytes in the lamina propria . To infect non-phagocytic epithelial cells S. Typhimurium employs genes within a region of the genome termed the Salmonella pathogenicity island 1 (SPI-1), which encodes a type three secretion system (T3SS) that injects SPI-1 encoded effector proteins into the host cell cytosol, stimulating cytoskeletal alterations, leading to membrane ruffling and internalization of Salmonella by pinocytosis . Some Salmonella then traverse to the basolateral side of the epithelial cell and exit via exocytosis into the interstitial space before being rapidly engulfed by phagocytes .
The phagocytes that engulf Salmonella in the lamina propria include neutrophils, which flood into the area in response to chemoattractants released by infected epithelial cells. In addition, Salmonella is taken up by resident antigen presenting cells (APC); macrophages (Mø) and dendritic cells (DC). Salmonella survives and replicates in Mø, which requires genes encoded within the Salmonella pathogenicity island 2 (SPI-2) . In contrast, S. Typhimurium only persists in murine DC without replicating [9, 10]. The response of bovine monocyte-derived Mø and DC to in vitro S. Typhimurium infection was found to differ . Transcripts of interleukin (IL) 12 and colony stimulating factor (CSF) 2 were up-regulated in DC, whilst IL10 was only up-regulated in Mø. In agreement with this pattern, IL12 and IL10 protein release was greater in DC and Mø, respectively, in response to heat-inactivated S. Dublin . The cell-specific release of different cytokines would alter the signalling to other immune cells, thus potentially affecting not only the innate, but also the development of the adaptive immune response at the site of infection. In turn, this may influence the course of the Salmonella infection.
To investigate early events which might lead to these differences we have compared the global transcriptional response of bovine monocyte-derived Mø and DC to early S. Typhimurium infection. S. Typhimurium infects Mø and DC in the lamina propria once the bacteria has passed across the epithelial layer. The bacteria can be internalized by these phagocytes by phagocytosis or SPI-1 mediated pinocytosis  and it is unclear which mechanism predominates in the lamina propria. Irrespective of the mode of entry, Salmonella survive within these cells in Salmonella containing vacuoles (SCVs) , which are fully mature approximately 1 h post infection  and Salmonella starts to replicate 3–4 h post infection . We investigated the transcriptional response of Mø and DC at a time between these two events, 2 h post infection, when S. Typhimurium is establishing a niche inside the cell. Furthermore, in an attempt to separate out the transcriptional response induced by the detection of PAMPs, e.g., LPS, flagellin, from that induced by the interaction with S. Typhimurium effector proteins, we compared the response of Mø and DC to live and inactivated S. Typhimurium. This may lead to insights into why inactivated Salmonella vaccines are relatively ineffective. Overall we found a similar transcriptional response by Mø and DC to live and inactivated S. Typhimurium. However, there were quantitative differences in the expression of a large proportion of the genes investigated at this early time point of infection, which may alter events down-stream during infection in both the infected cells and other immune cells.
Materials and methods
Cells were isolated from female Holstein–Friesian cattle (Bos taurus) maintained at The Roslin Institute, University of Edinburgh, UK. These Holstein–Friesian cattle were between 6 months to 5 years of age and kept on pasture. All experimental protocols were authorized under the UK Animals (Scientific Procedures) Act, 1986. In addition, The Roslin Institute’s Animal Welfare and Ethics Committee (AWEC) ensure compliance with all relevant legislation and promote the adoption and developments of the 3Rs.
Culture of monocyte-derived Mø
The bovine Mø used for the microarray and RT-qPCR studies were generated using different methods and derived from cells isolated from different animals. For the former, Mø were generated from peripheral blood essentially as described previously . Blood samples were collected aseptically by jugular venipuncture into acid citrate dextrose (ACD) and buffy coats were separated by centrifugation. These cells were washed three times with citrate buffer (30 mM citric acid, 0.6% NaCl, 3 mM KCl, 4.8 mM glucose), to remove fibrinogen. Peripheral blood mononuclear cells (PBMC) were separated by density gradient centrifugation on Lymphoprep (Axis-Shield) and resuspended at 5 × 106 cells/mL in Iscove’s modified Dulbecco’s medium (IMDM) (Invitrogen) supplemented with GlutaMax™ (Invitrogen), 25 mM HEPES, 100 IU/mL penicillin, 100 μg/mL streptomycin, 10 mM sodium pyruvate, 1% minimum essential medium (MEM) vitamins (Invitrogen), 1% non-essential amino acids (Invitrogen), 50 μM β-mercaptoethanol, 10 µg/mL gentamicin and 20% heat-inactivated foetal bovine serum (FBS) (Biosera). The PBMC were cultured in non-adherent Teflon bags for 7 days at 37 °C in 5% CO2, during which time the monocytes differentiated into Mø . Cells were then resuspended in fresh medium supplemented as above, except that the FBS concentration was reduced to 2%. Mø were purified by selective adherence for at least 6 h and non-adherent cells were thoroughly washed off. The purity of the Mø population was assessed by flow cytometric analysis using anti-human CD14, anti-bovine SIRPA, anti-bovine CD3 and anti-bovine CD21 antibodies using a CyAn ADP flow cytometer (Beckman Coulter) (data not shown) and exceeded 90%. The maturation of monocytes to Mø was confirmed by morphological examination and by RT-PCR analysis of selected Mø markers, e.g., CD16 and CD180, which showed strong expression in the generated Mø (data not shown).
The Mø used in the validation studies were generated as previously described  from peripheral PBMC cultured in flasks for 5 days and purified by adherence. Flow cytometric analysis using anti-human CD14, anti-bovine SIRPA, anti-bovine CD3 and anti-bovine CD21 antibodies confirmed that the Mø purity exceeded 95% (Additional file 1). The Mø were replated at 3 × 105 cells/mL in RPMI-1640 medium supplemented with 20% FBS and cultured for 48 h before stimulation to ensure that the cells had returned to a resting state.
Culture of monocyte-derived DC
The bovine DC used for the microarray and RT-qPCR studies were generated in a similar way but were derived from different animals. Blood was collected aseptically into ACD and PBMC were separated by density gradient centrifugation on Lymphoprep (Axis-Shield). CD14+ cells were separated by positive selection using microbeads conjugated with an anti-human CD14 antibody and the MACS system (Miltenyi Biotec). The purity of the resulting CD14+ cells was assessed by flow cytometry using an anti-bovine SIRPA monoclonal antibody (AbD Serotec) and exceeded 95% (data not shown). The cells were resuspended at 1 × 106 cells/mL in RPMI-1640 (Invitrogen) medium supplemented with 10% FBS, containing either recombinant bovine IL4 and recombinant CSF2 (kindly provided by Dr Gary Entrician, Moredun Research Institute, UK), at pre-determined optimal concentrations, or bovine DC growth kit (AbD Serotec). The cells were cultured at 37 °C in the humidified atmosphere of 5% CO2. After 5 days of culture the DC had acquired the morphology and phenotype of previously described monocyte-derived DC . To confirm the cell purity flow cytometric analysis using anti-human CD14, anti-bovine SIRPA, anti-bovine CD3 and anti-bovine CD21 antibodies was performed (Additional file 1). In addition, the generation of DC was confirmed by RT-PCR analysis of selected DC markers, e.g., CD1B, which showed strong expression in the generated DC (data not shown). The DC were replated at 3 × 105 cells/mL in RPMI-1640 medium supplemented with 10% FBS and DC growth kit and cultured for 48 h before stimulation to ensure that the cells had returned to a resting state.
The expression of cell surface molecules and DC/Mø associated transcripts was compared between the Mø and DC cultures (Additional file 1). These analyses confirmed that the culture methodologies employed had resulted in the generation of distinct cell populations.
Infection and stimulation of the cells with Salmonella
S. Typhimurium strain SL1344, verified by API-20E test (bioMerieux) (data not shown), was grown overnight at 37 °C in Luria–Bertani (LB) broth. The bacteria were washed once with phosphate buffered saline (PBS) and resuspended in fresh PBS. The bacteria were inactivated either by heating for 40 min at 80 °C or alternatively by treatment with 1% paraformaldehyde (PFA) for 1 h. The bacteria were then washed three times with PBS and resuspended in fresh PBS. Successful inactivation of bacteria was verified by the lack of colonies following overnight incubation on LB agar plates. Inactivated and live bacteria were added to the Mø and DC cultures to give a multiplicity of infection (MOI) of 50. Preliminary work using GFP-expressing S. Typhimurium SL1344 confirmed uptake of bacteria by over 90% Mø and DC using this experimental design (data not shown). The inocula numbers were confirmed by colony counts on LB agar plates. Control, resting samples were left untreated. After incubation for 2 h the APC were harvested and the RNA extracted. For addition studies RNA was extracted from Mø 0.5, 1, 2 and 3 h post stimulation. In addition, Mø were incubated for 8 h post stimulation and the supernatants used for ELISA.
Microarray experimental design
Mø and DC were prepared in parallel from six cattle. Each cell sample was cultured for 2 h at 37 °C in medium alone, exposed to live S. Typhimurium or exposed to heat-inactivated S. Typhimurium. Therefore a total of 36 samples were collected. The Affymetrix GeneChip® Bovine Genome Array, which measures the expression levels of over 23000 transcripts, was used to perform the transcriptomic experiment. The microarray data is publicly available at ArrayExpress and has the accession number E-TABM-878.
RNA preparation and microarray hybridization
RNA was extracted from Mø and DC using the RNeasy MiniKit (Qiagen) according to manufacturer’s instructions. The RNA concentration was measured using a NanoDrop ND100 Spectrophotometer and RNA quality was checked by Agilent 2100 Bioanalyzer. The RNA integrity number (RIN) exceeded 7 for all samples. Samples were concentrated using the Linear Acrylamide Precipitation System (Ambion). Two-cycle amplification and labelling of the RNA was performed using the GeneChip Expression 3′ Amplification Two-Cycle Target Labelling and Control Reagents Kit (Affymetrix), the IVT cRNA Cleanup Kit (Affymetrix) and the MEGAscript High Yield Transcription Kit (Ambion) according to the manufacturers’ instructions. The resulting copy RNA (cRNA) concentration and quality were measured by NanoDrop and Agilent Bioanalyzer respectively. 20 µg biotinylated cRNA was fragmented using 5 × Fragmentation Buffer (Affymetrix) and hybridized onto the Affymetrix GeneChip® Bovine Genome Array.
The hybridized microarrays were scanned using the Affymetrix scanner and the Affymetrix Genechip Operating software (GCOS) was used for the analysis of gene expression and expression clustering. Images were quantified with the command console software. Two slides failed the preliminary quality control (QC) and subsequent analysis, including principal component analysis (Additional file 2), identified issues with a number of other slides, which were removed from the analysis. As a result, four biological replicates were included in the analysis for each cell-type/condition.
Statistical and bioinformatic analysis of the microarray data
The microarray data were analysed in R (version 2.14.1) using the bioconductor 2.9 libraries . Briefly, after QC and normalization using the robust multiarray average (RMA) algorithm  MAS5. Absent calls were used to remove any probe-set absent across all the arrays. The paired sample analysis implemented in the significance analysis of microarrays (SAM) algorithm  was used to identify differentially regulated genes by comparing live and inactivated S. Typhimurium treated samples with the uninfected controls. Genes with false discovery rate (FDR)  less than 5% and absolute fold changes greater than 1.8 were considered significantly differently expressed. The Affymetrix Bovine Annotation (version 32) was used to annotate the differentially expressed probe-sets. If there was no designated description, the probe-sets were annotated manually using the consensus sequences, provided by Affymetrix, which had been used to design the probe-sets. The consensus sequences were blasted against the bovine genome (UMD3.1) and sequence databases e.g., NCBI.
The Database for annotation, visualization and integrated discovery (DAVID) [23, 24] was used to perform functional annotation analysis. Initially the analysis was carried out using the Affymetrix probe ID and setting the microarray as the background. However, due to the relative paucity of gene ontology (GO) annotation of bovine genes, the gene enrichment analysis was repeated using the HUGO approved gene symbols and the human genome was set as the background. The analysis was carried out to identify over-represented Biological Process GO terms.
PCR analysis of unannotated transcripts
RT-PCR analysis was carried out to investigate if selected unannotated transcripts represent novel splice variants. Oligonucleotides were designed to amplify products spanning the exonic sequence of neighbouring genes and the consensus sequence for the unannotated probe-sets provided by Affymetrix. One set of oligonucleotides was designed to investigate Bt.19462, the forward primer aligns to exon 3 of chemokine (C–C motif) ligand 5 (CCL5) (5′-TGCTGTGAAAGACCCTCAGT-3′) and the reverse primer aligns to the consensus sequence of Bt.19462 (5′-GCAGATTGAAGATGGAAGAGAA-3′). Two sets of oligonucleotides were designed to investigate Bt.17514; the first set consists of a forward primer which aligns to exon 1 of tumour necrosis factor, alpha-induced protein (TNFAIP) 3 (5′-CGAGAAGTCAGGAGGCTTG-3′) and the reverse primer aligns to the consensus sequence of Bt.17514 (5′-ACAACACCACCACCACCAC-3′). The second oligonucleotide pair consists of a forward primer that aligns to the consensus sequence of Bt.17514 (5′-GAGGATATGAGACTGCGGTGA-3′) and a reverse primer that aligns to exon 2 of TNFAIP3 (5′-CGGGTGTCGTAGCAAAGC-3′).
The oligonucleotide pairs were used to amplify products from bovine monocyte-derived Mø cDNA samples generated from total RNA using oligo(dT) primer and GoScript (Promega) according to the manufacturer’s instructions. The products were amplified using ABgene Taq polymerase (Thermo Scientific) and purified using QIAquick Gel Extraction Kit following the manufacturers’ instructions. Purified PCR fragments were cloned into pGEM-T easy vector (Promega) and the resulting plasmids purified using the Qiaprep miniprep kit (Qiagen) following the manufacturers’ instructions. The purified plasmids were sequenced using Big Dye Sequencing and T7 and SP6 oligonucleotides (Edinburgh Genomics).
RT-quantitative PCR (RT-qPCR) analysis of mRNA levels
The mRNA levels of the selected transcripts were quantified by RT-qPCR using different biological samples than those used for the microarray experiment. First strand cDNA was generated as described above and the qPCR was carried out using the Brilliant III ultra-fast SYBR Green Mastermix kit (Agilent). Oligonucleotides were designed for each gene using Primer3 [25, 26] and Netprimer (Biosoft International) software (Additional file 3). PCR products generated with each oligonucleotide set were sequenced to ensure amplification of the correct transcript. Reactions were carried out in 10 μL volumes containing; 1 × SYBR Green Master mix and reference dye, 0.5 μL forward and reverse primers at predetermined optimal concentrations and 2.5 μL cDNA diluted at 1:20 for all genes. Amplification and detection of products was carried out using a Mx3000P PCR machine (Stratagene) with the following cycle profile: 95 °C for 3 min followed by 50 cycles of 95 °C for 10 s and 60 °C for 22 s. The detection of a single product was verified by dissociation curve analysis. Each PCR experiment was carried out in triplicate and contained several non-template controls and a log10 dilution series of the representative standard. The relative quantities of mRNA were calculated using the method described by . The results for each target gene were normalized against the results for squamous cell carcinoma antigen recognized by T cells (SART1). SART1 was among a number of genes selected from analysis of the microarray data as being constitutively and moderately expressed in all the biological samples (data not shown). Further RT-qPCR analysis revealed that SART1 was the most suitable reference gene to use from the selected genes and other normalization genes frequently used in our laboratory (data not shown).
Knock-down of target genes by siRNA
Purified bovine monocyte-derived Mø were resuspended at 3×105 cells/mL in RPMI-1640 medium, supplemented with 20% FBS, dispensed into 12 well plates and cultured for 48 h before transfection of siRNA. siRNA duplexes specific for NLR family, pyrin domain containing 3 (NLRP3) (target sequence GTGTATATCTTCTTCCTCT) and Mediterranean Fever (MEFV) (target sequence GTTGCTTAATAAATCCTTA, described previously ) were designed and supplied by Sigma–Aldrich. The Mø were transfected with siRNA following the manufacturer’s protocol, initially generating a mix of 3 μL Lipofectamine RNAiMAX (Invitrogen) and 3 μL 20 μM siRNA in 200 μL Opti-MEM I reduced serum medium (Invitrogen). After 20 min incubation at room temperature the siRNA/Lipofectamine RNAiMAX mix was added to the Mø in 1 mL RPMI-1640 medium, supplemented with 20% FBS, giving a final concentration of 50 nM siRNA. Additional controls included in each experiment were Mø treated with Lipofectamine RNAiMAX only (transfection control) and untreated Mø (negative control). In addition, the AllStars negative control siRNA (Qiagen), which does not share homology with any known mammalian gene, was used as a non-target siRNA control. After 24 h the medium was replaced with fresh medium to remove the residual siRNA/Lipofectamine RNAiMAX mix. After a further 24 h cells were infected with live S. Typhimurium as described above.
Enzyme-linked immunosorbent assays (ELISA)
Supernatants were collected from Mø stimulated with live and PFA-inactivated S. Typhimurium for 8 h. Tumour necrosis factor (TNF) and IL1B protein levels in the supernatants were quantified by Bovine TNFα DuoSet ELISA (R&D Systems) and Bovine IL1β Screening Kit (Thermo Scientific Pierce) respectively, following the manufacturers’ instructions. Interferon gamma (IFNG) protein was quantified by ELISA using the mouse anti-bovine IFNG antibodies MCA2112 and MCA1783B (AbD Serotec) as coating and detecting antibodies respectively. Both antibodies were used at 2 μg/mL final concentration. The same protocol and buffers were used in this ELISA and the IL1B ELISA. The concentration of IFNG was determined using a standard curve of recombinant bovine IFNG (kindly provided by Dr. Jayne Hope, The Roslin Institute). All ELISAs were carried out using Nunc-Immuno Maxisorp 96 well plates, with all samples and standards in triplicate. The plates were read at 450 nm, with the reference values at a wavelength of 550 nm subtracted, using a Synergy HT plate reader (BioTek).
All statistical analysis was carried out using Minitab version 17. ELISA results were analysed by t test. The RT-qPCR data were transformed on the log2 scale before statistical analyses to stabilize the variance. Changes in gene expression compared to resting cells and differences in the response to live and inactivated Salmonella were analysed by t test with P value correction, using the Benjamini–Hochberg procedure , to adjust for multiple testing. Comparison of the response of DC and Mø was analysed by General Linear Model (GLM), fitting animals as a random effect, with cell-type (DC and Mø) and condition (unstimulated, live Salmonella infection and inactivated Salmonella stimulation) as fixed effects. Subsequent Fisher’s tests, with Benjamini–Hochberg adjustment of P values, were used to identify significant differences between cell types and conditions, as well as the interaction between cell-type and condition. Similarly, the time course data was analysed by GLM to allow repeated measures analysis.
Analysis of the microarray data
Summary of the differentially expressed genes identified by analysis of the microarray results
No. up-regulated genes
No. down-regulated genes
No. unannotated transcripts
All four gene lists contained genes that were represented by more than one probe-set, e.g., CASP8 and FADD-like apoptosis regulator (CFLAR) was represented by four probe-sets. Both the direction and magnitude of the differential expression were in agreement across the replicate probe-sets. The greatest fold up-regulation was observed in the Mø response to heat-inactivated bacteria, with 33.7-fold up-regulation of IL6. The observed down-regulation of gene expression did not reach that magnitude of change, the greatest down-regulation was observed in Mø in response to live infection, with a 4.0-fold decrease in thioredoxin interacting protein (TXNIP) expression.
Common transcriptional response
The Venn diagram (Figure 1) illustrated that 49 genes were differentially expressed in all four gene lists (Additional file 5) and therefore represent a common transcriptional response. Of these only one, paraneoplastic antigen MA1 (PNMA1), was down-regulated. CSF2 was consistently amongst the most up-regulated genes across all four gene lists. There was considerable variation in the level of differential expression observed in Mø and DC, the most extreme example of this is IL6, which was up-regulated by much higher levels in Mø, 17.7-fold and 33.7-fold in Mø_L and Mø_D respectively, compared to DC, 2.2-fold and 5.7-fold in DC_L and DC_D respectively. In addition, 14 of the common response genes (29%) exhibit more than 1.5-fold greater up-regulation in DC and/or Mø in response to inactivated S. Typhimurium compared to live bacteria. Again, IL6 is an example of this expression pattern.
RT-PCR of Mø cDNA with the oligonucleotide pair which spanned CCL5 exon 3 and Bt.19462 amplified four major products, which were cloned and sequenced [GenBank: KP213857–KP213860]. The largest product represented a 1082 nucleotide transcript that extended CCL5 exon 3 through to the Bt.19462 sequence (Figure 2A). The other transcripts had differing 3′ends of exon 3, none of which had the expected splice site motifs, and then included an extra exon which incorporated the Bt.19462 sequence (Figure 2A). The start of this additional exon had the correct splice site motif (AAG|N). All the transcript variants are predicted to result in the generation of the same peptide, with all the variability being in the 3′UTRs.
We were unable to generate a RT-PCR product using oligonucleotides that span exon 1 of TNFAIP3 and Bt.17514. However, oligonucleotides that anneal to Bt.17514 and exon 2 of TNFAIP3 amplified a 777 nucleotide product [GenBank: KP213861]. The first 586 nucleotides match Bt.17514, ending in an appropriate splice site motif (T|GT), and the remaining 191 nucleotides match the start of TNFAIP3 exon 2 (Figure 2B). Further work is required to investigate if Bt.17514 represents an alternative exon 1 for TNFAIP3, which would result in a truncated protein lacking the first 104 amino acids of TNFAIP3. Overall, the results confirm that Bt.17514 and Bt.19462 represent novel splice variants of TNFAIP3 and CCL5 respectively. For functional annotation analysis we have assumed that all unannotated transcripts represent splice variants of neighbouring genes.
Functional annotation clustering analysis using DAVID [23, 24] was carried out to investigate the common transcriptional response of DC and Mø to Salmonella stimulation. Only 50% of the genes were assigned GO terms when using the Affymetrix probe-IDs, due to the relative lack of annotation of bovine genes compared to those of human and mouse, which made meaningful functional analysis difficult. Therefore, the HGNC gene symbols of the differentially expressed genes were used in the DAVID analysis, which resulted in the annotation of almost all genes and provided much greater insights into the pathways involved in the common response.
Clusters of Biological Process GO terms over-represented in the common response gene list
Biological process GO terms
Regulation of cell communication
e.g., positive regulation of cell communication
positive regulation of signal transduction
positive regulation of protein kinase cascade
positive regulation of I-κB/NF-κB cascade
BST2, CD80, CFLAR, CSF2, EDN1, F3, IL6, PLK2, PTGS2, REL, TNF, TNFAIP3, VEGFA
Regulation of programmed cell death
e.g., regulation of programmed cell death
regulation of apoptosis
negative regulation of programmed cell death
BCL2A1, BTG2, CFLAR, CSF2, F3, GADD45B, IER3, IL6, NR4A1, PMAIP1, PTGS2, RYBP, TNF, TNFAIP3, TRAF1, VEGFA
e.g., inflammatory response
response to bacterium
BST2, CCL5, CCL20, CSF2, CXCL2, EDN1, F3, IL6, KDM6B, MEFV, NOS2, PTGS2, RNF19B, TNF, VEGFA
Specific transcriptional responses
Summary of genes annotated with the three major GO terms identified from functional analysis of the differentially expressed gene lists
CCL20, CCL5, CXCL2, F3, IL1A, IL1B, IL6, KDM6B, MEFV, NFKDID, NFKBIZ, PTX3, TNF, TNFAIP3
CCL20, CCL5, CCR7, CXCL2, F3, IL6, KDM6B, MEFV, NFKB1, OLR1, TNF, TNFAIP3
CD40LG, CCL20, CCL5, CCL8, CCR7, CXCL2, CFB, F3, IL1RN, IL1A, IL1B, IL10, IL6, KDM6B, MEFV, NFDBID, NFKBIZ, OLR1, PTX3, SELP, SAA2, TNF, TNFAIP3, TNFAIP6
CCL20, CCL5, CCL8, CCR4, CCR7, CXCL2, CFB, F3, FOS, IL1A, IL1B, IL10, IL6, KDM6B, MEFV, NFKBID, NFKBIZ, OLR1, PTX3, SELP, SAA2, TNF, TNFAIP3, TNFAIP6
Regulation of programmed cell death
BCL2A1, BIRC3, BTG2, CFLAR, CLCF1, CSF2, DUSP1, F3, GCLC, IER3, INHBA, IFIH1, IL1A, IL1B, IL6, NFKBIA, NR4A1, NR4A2, PMAIP1, PIM1, PTGS2, SOCS3, TNF, TNFAIP3, TRAF1, TXNIP, VEGFA
BCL2A1, BIRC3, BTG2, CFLAR, CSF2, F3, GCH1, GCLC, IER3, IL6, NFKB1, NFKBIA, NR4A1, NR4A2, PPIF, PMAIP1, PIM3, PLEKHF1, PTGS2, TNF, TNFAIP3, TRAF1, VEGFA
BCL2A1, BTG2, CD40LG, CFLAR, CLCF1, CD40LG, CSF2, DUSP1, F3, GDNF, IER3, INHBA, ID3, IFNG, IL1A, IL1B, IL10, IL6, LIG4, LTA, NR4A1, PPIF, PMAIP1, PIM1, PHLDA1, PTGS2, SOCS3, TNF, TNFAIP3, TRAF1, TXNIP, VEGFA
BCL2A1, BIRC3, BTG2, CFLAR, CLCF1, CSF2, DLC1, DUSP1, F3, GCLC, GDNF, IER3, INHBA, ID3, IFIH1, IL1A, IL1B, IL10, IL6, LIG4, MX1, NR4A1, PPIF, PMAIP1, PIM1, PLEKHF1, PHLDA1, PTGS2, SERPINB2, SOCS3, TNF, TNFAIP3, TRAF1, VEGFA
Regulation of transcription
BAZ1A, BHLHE41, BTG2, CD80, ETS2, GCLC, GZF1, ID2, INHBA, IL1B, IL6, IRF1, KDM6B, KLF5, NFIL3, NFKBIA, NFKBIZ, NR4A1, NR4A2, NR4A3, PIM1, PNRC1, PRDM1, RYBP, SKIL, TNF, TXNIP, VEGFA
BAZ1A, BTG2, CARHSP1, CD80, ETS2, ETV3, FOSB, FOXF1, GCLC, GZF1, ICAM1, IL6, IRF1, IRF8, KDM6B, KLF5, MAFF, NFATC1, NFIL3, NFKB1, NFKB2, NFKBIA, NR4A1, NR4A2, NR4A3, PRDM1, RYBP, SAFB2, SFRQ, SKIL, TLE1, TNF, VEGFA, ZBTB10
ATF3, BHLHE40, BHLHE41, BTG2, CD80, CEBPA, CEBPD, CSRNP1, EGR1, EHF, ETS2, GDNF, GZF1, HHEX, ICAM1, ID2, ID3, IFNG, IL1B, IL10, IL6, INHBA, IRF1, KDM6B, KLF13, MAFF, MED4, MTERF, NFATC1, NFIL3, NFKB2, NFKBIZ, NR4A1, PHF10, PPARGC1B, PIM1, PRDM1, RYBP, TNF, TXNIP, VEGFA, ZNF175, ZNF181, ZNF200, ZNF235, ZNF565, ZNF567, ZNF582, ZNF677, ZNF814
ATF3, BAZ1A, BHLHE41, BTG2, CCNL1, CD80, CEBPD, CSRNP1, EHF, ETS2, ETV3, FOS, FOSB, GCLC, GDNF, GTF2B, GZF1, ICAM1, ID1, ID2, ID3, IL1B, IL10, IL6, INHBA, IRF1, IRF4, KDM6B, KLF5, MAFF, NFATC1, NFKB2, NFKBIZ, NR4A1, PIM1, PRDM1, RYBP, SFPQ, SIK1, SKIL, TNF, VEGFA, ZNF132, ZNF582
The biological process GO terms associated with regulation of programmed cell death also exhibited cell-type differences. The majority of DC_L and DC_D genes assigned to this GO term were associated with the negative regulation of programmed cell death, e.g., 20 out of 31 genes in this cluster are anti-apoptotic. In contrast, the equivalent clusters in Mø gene lists contained a mix of GO terms associated with negative and positive regulation of programmed cell death. For example, the Mø_L gene list contains 18 genes with the GO term Negative regulation of programmed cell death, e.g., CSF2, BTG2, and also 15 genes with the GO term positive regulation of programmed cell death, e.g., inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3). These variations suggest that there are differences, even at early stages of infection, in how DC and Mø respond to stimulation with S. Typhimurium.
There are also differences in the Biological Process GO term clusters associated with the regulation of transcription, but these differences are associated with the stimuli and not the cell-type. DC_L and Mø_L genes are biased towards the positive regulation of transcription; for example, the Mø_L gene list contains 19 genes with the GO term positive regulation of transcription and only ten with the GO term negative regulation of transcription. In contrast, DC_D and Mø_D gene lists have more even numbers of negative and positive regulators of transcription. There is an overlap in the genes associated with the GO term negative regulation of transcription in DC_D and Mø_D, including FOSB and ets variant 3 (ETV3). This analysis suggests that at the relatively early time point post stimulation investigated in this study there are differences in the levels of transcription factors which could influence the expression of a large number of genes and thereby modify the response of the cells to live and inactivated Salmonella.
RT-qPCR analysis of differentially expressed genes associated with the GO terms of interest
Selection of genes for further analysis
The analysis of the microarray data identified three principal over-represented Biological Process GO terms from the differentially expressed gene lists; inflammatory response, regulation of transcription and regulation of programmed cell death. Intriguingly, these clusters differed with cell-type or stimuli. Therefore we decided to study these pathways in more detail, by investigating the expression of 31 genes, representative of these pathways, in a new set of samples derived from different animals. In addition, the new sample set differed from the previous set in the method used to generate Mø, which resulted in higher levels of cell purity. Furthermore, the S. Typhimurium was inactivated with PFA rather than by heat, thus retaining the surface protein structure, which may have been altered by heat and could have affected the detection of bacterial PAMPs by the APC.
The selected genes included several identified by analysis of the microarray data as being common response genes, e.g., chemokine (C-X-C motif) ligand 2 (CXCL2), IL6 and Bt.17514 (TNFAIP3), which are associated with inflammatory response and regulation of programmed cell death GO terms. In addition, genes which were differentially expressed with cell-type were included in the analysis, for example the Mø and DC specific genes ATF3 and NR4A2 respectively. Furthermore, genes exhibiting stimuli-specific differential expression were included, for example TXNIP and FOSB whose expression was only altered in response to live and inactivated S. Typhimurium respectively. Genes exhibiting cell-type specific differential expression in the earlier study ; CSF2, IL10 and IL12B, were also investigated. As previously mentioned CSF2 is among the common response genes identified in this study and not DC specific as previously found , while IL10 was identified as a Mø specific gene in agreement with the earlier study . IL12B was not included in any of the differentially expressed gene lits, although it is represented on the microarray. The expression of several genes, including jun B proto-oncogene (JUNB) and v-myc avian myelocytomatosis viral oncogene homolog (MYC) was also investigated. These genes exhibited differential expression, but below the 1.8-fold cut-off used to generate the differentially expressed gene lists. These were included to ascertain if the criteria used was too stringent.
Summary of qRT-PCR results quantifying the expression of genes associated with the three major GO terms investigated in DC and Mø infected with live Salmonella Typhimurium and stimulated with paraformaldehyde-inactivated S. Typhimurium
Average fold changes
9.8 ± 1.1a, b
6.1 ± 0.5a, b
8.5 ± 0.6a
6.7 ± 1.2a
64.0 ± 15.1a
51.0 ± 8.7a
81.3 ± 17.1a
60.0 ± 9.0a
53.1 ± 9.8a
43.1 ± 5.7a
51.8 ± 12.7a
59.2 ± 14.3a
641.0 ± 184.5a, b
403.3 ± 90.9a, b
443.5 ± 133.6a, b
270.9 ± 81.0a, b
182.0 ± 21.1a, b
119.3 ± 26.3a, b
257.2 ± 98.5a
198.2 ± 78.9a
308.9 ± 92.0a
558.2 ± 243.3a
633.8 ± 135.1a
548.3 ± 93.2a
260.6 ± 38.2a, b
147.1 ± 24.4a, b
521.6 ± 159.7a, b
209.9 ± 46.0a, b
0.9 ± 0.5
0.5 ± 0.5
0.2 ± 0.6
0.0 ± 0.6
3.8 ± 0.9a, b
1.5 ± 0.9b
4.2 ± 1.1a, b
1.6 ± 0.6a, b
−6.0 ± 1.3a, b
−9.9 ± 2.4a, b
−8.4 ± 1.0a
−9.8 ± 2.5a
71.2 ± 25.6a
102.1 ± 39.2a
103.0 ± 53.3a, b
77.2 ± 42.7a, b
3.1 ± 0.6a, b
1.5 ± 0.6b
2.6 ± 0.1a
2.2 ± 0.3a
−2.2 ± 0.3a
−2.4 ± 0.2a
−1.9 ± 0.2a
−2.4 ± 0.3a
589.3 ± 190.5a, b
25.1 ± 12.0a, b
22.0 ± 9.2a, b
2.1 ± 0.8b
1850.1 ± 415.0a
1353.8 ± 333.5a
3248.0 ± 1069.1a
2048.6 ± 611.3a
79.5 ± 20.7a
77.9 ± 31.2a
171.1 ± 89.3a
97.7 ± 37.9a
69.5 ± 20.4a
107.3 ± 31.8a
125.7 ± 44.6a
112.2 ± 18.6a
17 044.3 ± 16 476.7a, b
34 218.1 ± 32 873.8a, b
2591.0 ± 1834.2a
3340.4 ± 1966.5a
304.8 ± 57.8a, b
165.7 ± 28.0a, b
120.0 ± 48.2a, b
46.8 ± 19.0a, b
15.2 ± 4.1a, b
7.4 ± 0.9a, b
12.2 ± 1.0a, b
8.7 ± 1.0a, b
9.0 ± 0.8a
9.4 ± 1.3a
8.7 ± 1.5a
9.1 ± 1.4a
−2.9 ± 0.5a
−2.7 ± 0.3a
−3.1 ± 0.2a
−2.5 ± 0.3a
2.9 ± 0.3a
4.0 ± 0.5a
3.2 ± 0.3a, b
4.9 ± 0.5a, b
82.0 ± 27.7a
111.6 ± 15.8a
76.5 ± 14.3a, b
140.1 ± 16.2a, b
−0.5 ± 0.8b
−2.3 ± 0.5a, b
−0.9 ± 0.6
−1.7 ± 0.2a
4.4 ± 0.8a, b
1.1 ± 0.5b
2.6 ± 0.2a
1.9 ± 0.3a
−5.2 ± 0.7a
−4.1 ± 0.6a
−3.8 ± 0.6a
−3.2 ± 0.3a
353.7 ± 104.3a
368.6 ± 58.0a
551.6 ± 282.1a
452.5 ± 171.5a
683.3 ± 301.9a
927.7 ± 377.9a
9115.6 ± 5564.9a
9148.4 ± 6115.0a
IR, RT, CD
2.2 ± 0.8a, b
1.8 ± 0.7b
3.0 ± 0.3a, b
0.9 ± 0.5b
−1.3 ± 0.1a
−0.8 ± 0.4
−0.4 ± 0.5
−1.1 ± 0.0a
There were no obvious cell-type or stimuli-specific differences in the transcriptional response of Mø and DC with respect to the 15 investigated genes, except TXNIP. However, further statistical analysis identified significant stimuli-specific differences in the expression of eight genes (53%) in the response of DC and/or Mø at the level of fold change (Table 4; b). Six of the investigated genes; Bt.19462 (CCL5), CSF2, CXCL2, IFNG, ISG15 ubiquitin-like modifier (ISG15) and TXNIP, were more highly up-regulated in response to live S. Typhimurium than inactivated bacteria, e.g., CXCL2 was up-regulated on average 260.6-fold and 521.6-fold in DC and Mø infected with live S. Typhimurium respectively compared with 147.1-fold and 209.9-fold average fold change in DC and Mø respectively stimulated with inactivated S. Typhimurium. In contrast, IL12B and MEFV was more highly up-regulated, in DC and Mø respectively, in response to stimulation with inactivated S. Typhimurium than live S. Typhimurium (Table 4).
Regulation of transcription
Fifteen genes associated with the regulation of transcription were investigated, the majority of which are transcription factors, e.g., FOSB, hematopoietically expressed homeobox (HHEX). Fourteen of the investigated transcription-associated genes exhibited differential expression in response to live or inactivated S. Typhimurium in Mø and/or DC compared to resting cells (Table 4; RT). The exception was ETS1, the expression of which did not significantly alter in either cell-type, in agreement with the microarray data. The four genes, which according to the microarray data analysis exhibited cell-type specific differential expression; ATF3, ID3, ligase IV, DNA, ATP-dependent (LIG4) and NR4A2, were all found to be significantly differentially expressed in both cell types in response to live and inactivated S. Typhimurium, except NR4A2 was not significantly differentially expressed in DC stimulated with inactivated S. Typhimurium (Table 4).
Several genes exhibited stimuli-specific differential expression in response to live and inactivated S. Typhimurium, especially in DC. Five genes; FOSB, ID2, NR4A2, TXNIP and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta (YWHAB), were differentially expressed in DC in response to live S. Typhimurium but not inactivated bacteria. As mentioned above, TXNIP expression also exhibited similar stimuli-specific differential expression in Mø. In addition, live S. Typhimurium infection of DC and Mø induced a very variable effect on MYC expression across the biological replicates, whilst stimulation with inactivated S. Typhimurium induced down-regulation of MYC, by on average −2.3-fold and −1.7-fold in DC and Mø respectively.
Therefore, although the microarray and RT-qPCR results do not agree for individual genes, the RT-qPCR does support the observation of a stimuli-specific response associated with the regulation of transcription, especially in DC. This was further high-lighted when the statistical analysis of the RT-qPCR data revealed that there were more subtle differences between the response of DC and Mø to live and inactivated S. Typhimurium, with ten genes (67%) exhibiting statistically significant stimuli-specific differences in the average fold change values in DC and/or Mø (Table 4; b). In the majority of cases; ATF3, FOSB, ID1, ID2, JUNB, NR4A2 and TXNIP, a greater change in expression was observed in response to live S. Typhimurium infection, e.g., JUNB was up-regulated to a higher degree in DC and Mø infected with live S. Typhimurium, on average 15.2-fold and 12.2-fold respectively, than in cells stimulated with inactivated S. Typhimurium, on average 7.4-fold and 8.7-fold respectively (Table 4). The change in gene expression was greater in response to inactivated S. Typhimurium for three genes; LIG4, HHEX and MYC, described above. HHEX was down-regulated to a significantly higher degree in DC in response to inactivated S. Typhimurium, on average 9.9-fold, compared to live S. Typhimurium, on average 6.0-fold (Table 4). LIG4 was up-regulated to a significantly higher level in Mø in response to inactivated S. Typhimurium, on average 4.9-fold, compared to live S. Typhimurium, on average 3.2-fold (Table 4).
Regulation of programmed cell death
Nineteen genes included in the RT-qPCR analysis have been assigned Biological Process GO terms associated with the regulation of programmed cell death. The majority of the investigated genes are also associated with the over-represented GO terms regulation of transcription, e.g., LIG4, NR4A2, and inflammatory response, e.g., IFNG, IL6. Eight of the investigated genes are associated with the negative regulation of cell death; baculoviral IAP repeat containing 3 (BIRC3), CSF2, ID1, IL1B, LIG4, MYC, NR4A2 and TNFAIP3 and all of these, except MYC, were up-regulated in Mø and DC in response to live and inactivated S. Typhimurium, although this did not reach statistical significance for NR4A2 in DC stimulated with inactivated bacteria (Table 4; CD). Four genes; pleckstrin homology domain containing, family F (with FYVE domain) member 1 (PLEKHF1), ID3, IL12B and TXNIP, are associated with the positive regulation of programmed cell death. Interestingly, two of these, PLEKHF1 and ID3, were down-regulated across all cell types and S. Typhimurium stimuli, possibly promoting cell survival along with the up-regulation of the negative regulators of cell death. TXNIP, as described previously, was up-regulated in Mø and DC only in response to live S. Typhimurium infection, suggesting that the bacteria may be altering the survival state of the infected cell. Overall there is little evidence from the RT-qPCR data to support the cell-type specific difference in the regulation of programmed cell death suggested by analysis of the microarray data. The negative and positive regulators of programmed cell death investigated were similarly expressed in both cell types.
Further investigation of the transcriptional response of Mø
Transcriptional differences between DC and Mø
Direct comparison of mRNA levels for investigated genes in DC and Mø
Average fold differences between cell types
Live S. Typhimurium
Inactivated S. Typhimurium
P = 0.006
1.1 ± 1.3
2.7 ± 0.3a
2.0 ± 0.4
P = 0.023
1.6 ± 0.6
1.1 ± 0.4
1.4 ± 0.6
P < 0.001
1.7 ± 0.4
1.6 ± 0.1a
1.3 ± 0.1
3.3 ± 0.9
15.4 ± 6.7a
4.2 ± 1.3
P < 0.001
3.2 ± 1.9
4.0 ± 1.0a
3.6 ± 0.8a
P = 0.019
8.3 ± 7.4
7.7 ± 5.5
8.0 ± 5.0
7.5 ± 3.9a
1.1 ± 0.2
1.5 ± 0.3
P < 0.001
1.8 ± 0.2a
1.1 ± 0.8a
1.7 ± 0.3a
P = 0.019
1.2 ± 0.1
2.1 ± 0.3a
1.0 ± 0.1
P < 0.001
2.8 ± 1.7
4.2 ± 1.8a
2.3 ± 0.2
0.4 ± 1.4
0.6 ± 0.3
1.2 ± 0.4a
P = 0.024
0.2 ± 0.6
0.1 ± 0.5
0.4 ± 0.4
Transcription of the Mediterranean fever gene
Bovine MEFV had not been characterized previously; therefore we set about generating the full length MEFV transcript sequence for B. taurus and Bos indicus (Additional file 6). Three splice variants of bovine MEFV were identified [GenBank: JX560181–JX560190], which were all up-regulated in response to stimuli. The three splice variants are predicted to result in two protein isoforms, which differ at the extreme C-terminus (Figure 5B). Analysis of the predicted protein domains using the web-based SMART tool  suggests that, similar to murine MEFV, due to frame-shift mutations the bovine orthologue lacks the terminal PRY/SPRY (B30.2) domain of human MEFV (Figure 5B), which contains most mutations associated with FMF. Attempts to detect bovine MEFV protein using four different anti-human MEFV antibodies, all predicted to cross-react with bovine MEFV, failed to detect protein by Western blot (data not shown).
Intestinal Mø and DC play important roles during S. Typhimurium infection either as effector cells, removing and destroying invading bacteria, or as activator cells, signalling to other parts of the immune system. However, the ease with which both cell types can take up bacteria also means that these cells provide a safe haven for S. Typhimurium and can potentially be hijacked as a Trojan horse. Our present study aimed to extend previous work suggesting that the response of both cell-types to infection with S. Typhimurium differed , resulting in distinct subsequent inflammatory responses of the gut to S. Typhimurium infection.
Analysis of the microarray data revealed that several 100 genes were differentially expressed in DC and/or Mø in response to live and inactivated S. Typhimurium. Many of the differentially expressed genes have previously been shown to be modulated during activation or infection of Mø and DC. Interestingly, we identified a common transcriptional response that overlaps with the common response of human Mø to various bacterial pathogens, e.g., up-regulation of IL6, TNF, CSF2, CXCL2, CCL5, interferon regulatory factor 1 (IRF1), ZFP36 ring finger protein (ZFP36), prostaglandin-endoperoxide synthase 2 (PTGS2), ISG15, poliovirus receptor (PVR) and phosphodiesterase 4B, cAMP-specific (PDE4B) . In each gene list approximately 5% of the differentially expressed transcripts were not annotated, having been identified by probes designed from unannotated EST sequences that do not align to exonic sequences, but rather align to intronic or intergenic regions of the current bovine genome assembly. Further analysis of two of these unannotated sequences confirmed that they represent novel transcript variants. The novel transcript of CCL5 exhibited similar patterns and levels of up-regulation across the gene lists as the recognized CCL5 transcript. The second novel sequence investigated was identified as a novel transcript of TNFAIP3, which is not represented by any annotated probe-set on the Affymetrix bovine microarray. Therefore, when analyzing gene lists it is worthwhile investigating and identifying unannotated transcripts, as they can improve knowledge of the transcriptome, especially when investigating species with relatively poor genome annotation.
Functional analysis of the differentially expressed gene lists identified a range of biological processes that were affected by S. Typhimurium stimulation. Given their importance for the subsequent immune response, we concentrated on three of these processes; regulation of programmed cell death, inflammatory response and regulation of transcription. These were among the most over-represented terms identified by the analysis and exhibited cell-type and stimuli-specific differences. To investigate these pathways in more detail, we quantified the expression of a selection of representative genes by RT-qPCR, which illustrated the considerable overlap in the response to Mø and DC to live and inactivated S. Typhimurium. All the investigated genes were expressed in both cell types, even though several were not identified as such from analysis of the microarray data, and they were similarly modulated in response to stimuli, i.e., were consistently up- or down-regulated in both cell-types. Only more in-depth analysis of the degree of differential expression, i.e., the average fold changes, reveals variation in the response. Therefore, we have focussed on our RT-qPCR analysis to provide insights into the differential response of DC and Mø to live and inactivated S. Typhimurium, assuming that the cell-type and stimuli-specific differences observed in the sub-set of investigated genes are indicative of the global response of the cells.
Nearly 40% of the investigated genes were differentially expressed between DC and Mø. The microarray and RT-qPCR analysis revealed that there was a greater inflammatory response in Mø than DC, both in the number of genes differentially expressed as well as in the degree of differential expression observed, in-line with the proposed functions of both cell types. In a recent study comparing the response of porcine monocyte-derived Mø and DC to S. Typhimurium infection, Mø again showed a more pro-inflammatory response than DC, e.g., with the production of higher levels of IL1A and IL1B . Furthermore, murine bone-marrow derived Mø produced more IL6 and TNF than bone-marrow derived DC . Therefore, Mø seem to play, at least initially, a major role in alerting and attracting other immune cells to the site of infection. In contrast, we could not verify differences in the expression levels of IL10, CSF2 and IL12B identified in an earlier study  as exhibiting cell-type differences in expression during S. Typhimurium infection. Given that the methodology of this earlier study was very different than that used in our study, in terms of time-point of analysis as well as MOI of S. Typhimurium used , this may not be surprising. Indeed, after 24 h stimulation the DC have a more mature phenotype with increased surface expression of CD40, CD80, CD86 and MHC class II  and are therefore considerably different to those investigated in our study.
The notable exception to the pattern of higher cytokine expression by Mø than DC was IFNG, which was found to be transcribed at higher levels in bovine DC than Mø. There is now good evidence to support the view that Mø and DC produce IFNG in response to various stimuli [36–38]. IFNG is up-regulated at the mRNA level in bovine Mø stimulated with Escherichia coli-derived LPS but not Mycobacterium bovis-derived purified protein . Mø were found to be the predominant IFNG producing cell in the spleen during systemic S. Typhimurium infection of mice . If the differential transcription of IFNG observed in bovine DC and Mø results in differences in protein secretion, the uptake of S. Typhimurium by either cell-type could have a huge impact on the course of infection, as IFNG is a major activator of inflammation, driving direct bacterial killing via oxygen-radical production, enhancing phagocytosis, as well as enhancing antigen presentation . IFNG protein has previously been shown to be produced by human monocyte-derived Mø and DC in response to S. Typhimurium infection, albeit at a later time-point of infection . In these experiments production of IFNG by Mø was inhibited by anti-IFNA, anti-IL12 and anti-IL18 antibodies, while IFNG production by DC depended on IL12 and IL18 . In addition, IL12, IL18 and IL27 play an important role in regulating IFNG production by Mø in response to M. tuberculosis infection . However, IL12B mRNA levels were significantly higher in DC in response to inactivated S. Typhimurium compared to the response to live bacteria, the opposite pattern of IFNG expression. IL27 is not represented on the microarray, while IFNA and IL18, which are represented, were not included in any of the differentially expressed gene lists. Whether these cytokines are involved in the regulation of such early transcription of IFNG in APC requires further investigation.
The comparison of the transcriptional response of Mø and DC by RT-qPCR suggests that it is not the expression of cell-specific genes but the balance of expression of common genes that defines the response of each cell type. This similarity may not be surprising considering that both cell populations are derived in vitro from blood monocytes. Meta-analysis of murine transcriptome data has found that amongst the investigated immune cells bone-marrow derived Mø and DC form a single cluster, which is distinct from classical in vivo isolated DC, e.g., splenic DC . However, there is evidence that the intracellular interactions of S. Typhimurium with Mø and DC differ. Mutations in SPI proteins that reduce S. Typhimurium survival in Mø do not affect survival in DC . Furthermore, S. Typhimurium survives in Mø and DC, but only appears to replicate in Mø [9, 10]. Previous studies have found more profound differences in the response of Mø and DC to Salmonella infection, however they have predominantly looked at later time-points when the infection is established and DC have matured [11, 34]. The more subtle changes in gene expression observed in our study, especially those of transcription factors, may account for these later, more obvious, transcriptional differences.
In addition to cell-type differential expression, the RT-qPCR data suggests that the transcriptional response to live and inactivated bacteria differed substantially. Again, in most cases, the response differed in the degree of expression change after stimulation rather than in the expression of a different subset of genes. The average fold change observed in DC and/or Mø in response to live and inactivated S. Typhimurium was significantly different in over half the investigated genes (Table 4). These differences were observed in genes associated with all three studied GO terms; however the greatest number was associated with the regulation of transcription. Two-thirds of the investigated transcription factors exhibited stimuli-specific differential expression. Furthermore, in DC one-third of the investigated transcription factors were only differentially expressed in response to live infection. This suggests that either Salmonella targets this cellular process, presumably to promote survival, or the host cell recognizes the difference between live and inactivated Salmonella and responds accordingly by altering the concentration of transcription factors.
Analyzing the differences in more detail, ATF3 was among the transcription factors whose up-regulation was significantly higher in response to live infection than stimulation with inactivated Salmonella in both cell types. Homodimers of this basic leucine zipper transcription factor are negative regulators of inflammation and have been shown to repress the expression of IL6, IL12B and TNF . However, stimuli-specific expression of these genes was not observed in this study. ATF3 dimerises with JUN and FOS family members and the resulting heterodimers can activate or repress the transcription of different genes, e.g., ATF3/JUN dimers enhance the transcription of IFNG in Th1 lymphocytes . The expression of ATF3 is triggered by a wide range of stimuli, including LPS  as well as IFNG . ATF3 has previously been shown to be rapidly up-regulated in HeLa cells during S. Typhimurium infection due to the activity of SPI-1 effector proteins [48, 49]. Thus, the expression of IFNG and the presence of SPI-1 effector proteins may account for the enhanced expression of ATF3 observed in response to live S. Typhimurium infection.
Analysis of the microarray data suggested that a large proportion of the common response genes exhibited average fold increases over 1.5-fold higher in response to inactivated S. Typhimurium rather than live bacteria in DC and/or Mø. Four of these genes were included in our RT-qPCR panel; ISG15, Bt.17514 (TNFAIP3), IL6 and MEFV. Of these, a similar pattern of stimuli-specific differential expression was only observed with MEFV in Mø. The pattern of MEFV expression led us to hypothesize that MEFV expression is suppressed by S. Typhimurium. MEFV binds to inflammasome components, caspase 1 and PYD and CARD domain containing (PYCARD), and has been postulated to regulate inflammasome activity or act as an inflammasome sensor [32, 50]. Indeed, human, murine and bovine MEFV orthologues all contain the N-terminal Pyrin domain and therefore can interact with inflammasomes via PYCARD. In contrast, bovine MEFV, similar to the murine orthologue, lacks the B30.2 domain present in the MEFV of human and primates. In both murine and bovine MEFV the B30.2 domain has been lost due to frame-shift mutations, which may result from this domain becoming obsolete or it may have been actively lost as the result of adaptation to changes in pathogen exposure. As human MEFV can interact directly with caspase 1 via the B30.2 domain , it may play a different role in the function of inflammasomes compared to murine and bovine MEFV. Our experiments using siRNA knock-down failed to confirm a role for bovine MEFV during S. Typhimurium infection, in line with previous work investigating IL1B production after S. Typhimurium infection of bone marrow-derived Mø derived from MEFV −/− mice . In contrast, the knock-down studies show that the NLRP3 inflammasome is functional in bovine Mø during S. Typhimurium infection. Interestingly, the NLRP3 inflammasome is inhibited during S. Typhimurium infection of murine cells , illustrating a clear difference in the response of cells from different mammalian species. Our failure to detect MEFV with four antibodies predicted to cross-react with bovine MEFV by Western blot could imply that the transcripts may not be translated into mature protein, hence the lack of an effect with knock-down. Alternatively, effects on the inflammasome may be an irrelevant read-out to investigate. Human and murine MEFV are multifunctional, interacting with several proteins that affect NF-κB activation, migration, apoptosis and cytoskeletal signalling . However, we did not observe differences in S. Typhimurium survival in our MEFV knock-down studies (data not shown). Therefore we conclude that the present data does not support the hypothesis that MEFV expression is specifically targeted by S. Typhimurium to promote bacterial survival. The observed stimuli-specific expression may be an off-target effect, due to the bacteria modulating the expression of other genes of importance to the host-pathogen interaction, which are transcriptionally regulated by the same processes as MEFV.
The time course data suggests that stimuli-specific differential expression appears approximately 1–2 h post stimulation (Figure 6), when the Salmonella is establishing a niche within SCVs . During the infection of Mø over 900 S. Typhimurium genes were found to be differentially expressed, the majority by 4 h post infection . To date over 20 S. Typhimurium proteins have been shown to interact with host cell proteins . Several of these bacterial proteins, e.g., SopB and SpvC, can modulate the JNK and MAPK signalling pathways and the activity of NF-κB and AP-1 transcription factors [54–57]. Many of the investigated genes are regulated by these pathways, e.g., NF-κB regulates the expression of many cytokines and regulators of apoptosis [58, 59], which would account for the observed differences in the transcriptional response. However, it is unclear if these effector proteins are acting on the host cell at the time when the stimuli-specific differential expression observed in this study becomes apparent. SPI-2 proteins which form the structure of the T3SS and genes regulating the expression of SPI-2 genes were found to be expressed at relatively high levels 2 h post infection , but the secreted effector proteins investigated were maximally expressed later in infection, with relatively low expression at 2 h . Furthermore, SPI-1 protein expression is suppressed in S. Typhimurium cultures grown to stationary phase , which were used in this study. However, their expression is not totally ablated and levels of several effector proteins increases after phagocytosis . Therefore, although there is limited expression of Salmonella effector proteins at the time point investigated, it is possible that these proteins could account for the observed changes of the transcriptional response of DC and Mø to S. Typhimurium infection and further investigation involving S. Typhimurium mutants is warranted to identify these proteins. Alternatively, the different response of DC and Mø to live and inactivated bacteria may result from the host cell distinguishing between live and dead stimuli and responding accordingly. The observed differences in the investigated subset of differentially expressed genes may have a profound effect on the down-stream response of the infected cell and other cells of the immune response. This may partially account for why killed vaccines are less effective than live vaccines. They elicit poor cell-mediated immunity , which may start from the initial response of the phagocytic APC which detects the killed bacteria. Further work is required to elucidate the important aspects of this initial response, modulation of which may enhance the down-stream immune response to vaccines.
In summary, transcriptional analysis of the response of bovine monocyte-derived DC and Mø has revealed a very similar early response by both cell-types to Salmonella infection and also to stimulation with inactivated Salmonella. However, more in-depth analysis revealed more subtle differences in the intensity of the transcriptional response, which would influence the response of the host cell at later time points and could also modulate the immune response of the infected animal.
The authors declare that they have no competing interests.
AK and KJ planned the microarray experiment and conducted the majority of the lab based work. CZ and OA aided in the RT-qPCR analysis. IJG analysed the microarray data. KJ drafted the manuscript. MPG provided the Salmonella expertise and resources. EJG and DW conceived the initial idea of the study, and participated in its design and coordination. All authors helped to draft the manuscript. All authors read and approved the final manuscript.
We would like to thank Daphne Mouzaki from ARK Genomics and Dave Waddington from The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh for statistical analysis of the microarray data and Helen Brown from The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh for her advice on the statistical analysis of the RT-qPCR data. We would like to thank Mark Fell from ARK Genomics for helping with submission of the experiment to ArrayExpress. We would also like to thank Gary Entrician from the Moredun Research Institute for providing the recombinant bovine IL4 and CSF2 through the BBSRC funded Immunology Toolbox . This project was funded by EADGENE (EU contract no. FOOD-CT-2004-506416). EJG and KJ were also supported by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant (ISP3 Innate Immunity & Endemic Disease) [BB/J004227/1].
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