Schematic diagram illustrating the alignment of unannotated sequences and nearby genes. Gene structure of bovine A chemokine (C–C motif) ligand 5 (CCL5) and B tumour necrosis factor, alpha-induced protein 3 (TNFAIP3) and the position of the unannotated sequences Bt.19462 and Bt.17514 respectively. Open boxes denote known gene exons and grey boxes represent unannotated sequence. Solid lines denote intronic sequence. Dotted lines indicate predicted introns to accommodate unannotated sequences. Arrows illustrate the position of primers used to investigate if the unannotated transcripts represent previously unknown splice variants. Stippled boxes illustrate the sequenced novel transcripts and contain the relevant accession number.