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Table 4 Summary of qRT-PCR results quantifying the expression of genes associated with the three major GO terms investigated in DC and Mø infected with live Salmonella Typhimurium and stimulated with paraformaldehyde-inactivated S. Typhimurium

From: Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells

Gene symbol GO term Average fold changes
DC_L DC_D Mø_L Mø_D
ATF3 RT 9.8 ± 1.1a, b 6.1 ± 0.5a, b 8.5 ± 0.6a 6.7 ± 1.2a
BIRC3 CD 64.0 ± 15.1a 51.0 ± 8.7a 81.3 ± 17.1a 60.0 ± 9.0a
Bt.17514 (TNFAIP3) IR, CD 53.1 ± 9.8a 43.1 ± 5.7a 51.8 ± 12.7a 59.2 ± 14.3a
Bt.19462 (CCL5) IR, CD 641.0 ± 184.5a, b 403.3 ± 90.9a, b 443.5 ± 133.6a, b 270.9 ± 81.0a, b
CSF2 IR, CD 182.0 ± 21.1a, b 119.3 ± 26.3a, b 257.2 ± 98.5a 198.2 ± 78.9a
CSF3 IR 308.9 ± 92.0a 558.2 ± 243.3a 633.8 ± 135.1a 548.3 ± 93.2a
CXCL2 IR 260.6 ± 38.2a, b 147.1 ± 24.4a, b 521.6 ± 159.7a, b 209.9 ± 46.0a, b
ETS1 RT, CD 0.9 ± 0.5 0.5 ± 0.5 0.2 ± 0.6 0.0 ± 0.6
FOSB RT 3.8 ± 0.9a, b 1.5 ± 0.9b 4.2 ± 1.1a, b 1.6 ± 0.6a, b
HHEX RT −6.0 ± 1.3a, b −9.9 ± 2.4a, b −8.4 ± 1.0a −9.8 ± 2.5a
ID1 RT, CD 71.2 ± 25.6a 102.1 ± 39.2a 103.0 ± 53.3a, b 77.2 ± 42.7a, b
ID2 RT 3.1 ± 0.6a, b  1.5 ± 0.6b 2.6 ± 0.1a 2.2 ± 0.3a
ID3 RT, CD −2.2 ± 0.3a −2.4 ± 0.2a −1.9 ± 0.2a −2.4 ± 0.3a
IFNG IR, CD 589.3 ± 190.5a, b 25.1 ± 12.0a, b 22.0 ± 9.2a, b 2.1 ± 0.8b
IL1B IR, CD 1850.1 ± 415.0a 1353.8 ± 333.5a 3248.0 ± 1069.1a 2048.6 ± 611.3a
IL6 IR, CD 79.5 ± 20.7a 77.9 ± 31.2a 171.1 ± 89.3a 97.7 ± 37.9a
IL10 IR, CD 69.5 ± 20.4a 107.3 ± 31.8a 125.7 ± 44.6a 112.2 ± 18.6a
IL12B IR, CD 17 044.3 ± 16 476.7a, b 34 218.1 ± 32 873.8a, b 2591.0 ± 1834.2a 3340.4 ± 1966.5a
ISG15 IR 304.8 ± 57.8a, b 165.7 ± 28.0a, b 120.0 ± 48.2a, b 46.8 ± 19.0a, b
JUNB RT 15.2 ± 4.1a, b 7.4 ± 0.9a, b 12.2 ± 1.0a, b 8.7 ± 1.0a, b
KLF5 RT 9.0 ± 0.8a 9.4 ± 1.3a 8.7 ± 1.5a 9.1 ± 1.4a
KLF13 RT −2.9 ± 0.5a −2.7 ± 0.3a −3.1 ± 0.2a −2.5 ± 0.3a
LIG4 RT, CD 2.9 ± 0.3a 4.0 ± 0.5a 3.2 ± 0.3a, b 4.9 ± 0.5a, b
MEFV IR 82.0 ± 27.7a 111.6 ± 15.8a 76.5 ± 14.3a, b 140.1 ± 16.2a, b
MYC RT, CD −0.5 ± 0.8b −2.3 ± 0.5a, b −0.9 ± 0.6 −1.7 ± 0.2a
NR4A2 RT, CD 4.4 ± 0.8a, b 1.1 ± 0.5b 2.6 ± 0.2a 1.9 ± 0.3a
PLEKHF1 CD −5.2 ± 0.7a −4.1 ± 0.6a −3.8 ± 0.6a −3.2 ± 0.3a
TNF IR, CD 353.7 ± 104.3a 368.6 ± 58.0a 551.6 ± 282.1a 452.5 ± 171.5a
TNFAIP6 IR 683.3 ± 301.9a 927.7 ± 377.9a 9115.6 ± 5564.9a 9148.4 ± 6115.0a
TXNIP IR, RT, CD 2.2 ± 0.8a, b 1.8 ± 0.7b 3.0 ± 0.3a, b 0.9 ± 0.5b
YWHAB RT, CD −1.3 ± 0.1a −0.8 ± 0.4 −0.4 ± 0.5 −1.1 ± 0.0a
  1. The results are expressed as the average fold change in mRNA levels in DC treated with live (DC_L) and inactivated (DC_D) S. Typhimurium and Mø treated with live (Mø _L) and inactivated (Mø _D) S. Typhimurium compared to the uninfected DC or Mø ± standard errors.
  2. aIndicates when the mean fold change values were significantly different from resting cells by t test corrected for multiple testing (Benjamini–Hochberg) (P < 0.05).
  3. bDenotes when the mean fold change measured in DC and/or Mø is significantly different in the response to live and inactivated Salmonella by t test corrected for multiple testing (Benjamini–Hochberg) (P < 0.05). The GO term column indicates which of the three major GO terms the gene is associated with; inflammatory response (IR), regulation of transcription (RT) and regulation of programmed cell death (CD).