Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells

Jensen, Kirsty; Gallagher, Iain J.; Kaliszewska, Anna; Zhang, Chen; Abejide, Oluyinka; Gallagher, Maurice P.; Werling, Dirk; Glass, Elizabeth J.

doi:10.1186/s13567-016-0328-y

Veterinary Research

Table 4 Summary of qRT-PCR results quantifying the expression of genes associated with the three major GO terms investigated in DC and Mø infected with live Salmonella Typhimurium and stimulated with paraformaldehyde-inactivated S. Typhimurium

From: Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells

Gene symbol	GO term	Average fold changes
Gene symbol	GO term	DC_L	DC_D	Mø_L	Mø_D
ATF3	RT	9.8 ± 1.1^a, b	6.1 ± 0.5^a, b	8.5 ± 0.6^a	6.7 ± 1.2^a
BIRC3	CD	64.0 ± 15.1^a	51.0 ± 8.7^a	81.3 ± 17.1^a	60.0 ± 9.0^a
Bt.17514 (TNFAIP3)	IR, CD	53.1 ± 9.8^a	43.1 ± 5.7^a	51.8 ± 12.7^a	59.2 ± 14.3^a
Bt.19462 (CCL5)	IR, CD	641.0 ± 184.5^a, b	403.3 ± 90.9^a, b	443.5 ± 133.6^a, b	270.9 ± 81.0^a, b
CSF2	IR, CD	182.0 ± 21.1^a, b	119.3 ± 26.3^a, b	257.2 ± 98.5^a	198.2 ± 78.9^a
CSF3	IR	308.9 ± 92.0^a	558.2 ± 243.3^a	633.8 ± 135.1^a	548.3 ± 93.2^a
CXCL2	IR	260.6 ± 38.2^a, b	147.1 ± 24.4^a, b	521.6 ± 159.7^a, b	209.9 ± 46.0^a, b
ETS1	RT, CD	0.9 ± 0.5	0.5 ± 0.5	0.2 ± 0.6	0.0 ± 0.6
FOSB	RT	3.8 ± 0.9^a, b	1.5 ± 0.9^b	4.2 ± 1.1^a, b	1.6 ± 0.6^a, b
HHEX	RT	−6.0 ± 1.3^a, b	−9.9 ± 2.4^a, b	−8.4 ± 1.0^a	−9.8 ± 2.5^a
ID1	RT, CD	71.2 ± 25.6^a	102.1 ± 39.2^a	103.0 ± 53.3^a, b	77.2 ± 42.7^a, b
ID2	RT	3.1 ± 0.6^a, b	1.5 ± 0.6^b	2.6 ± 0.1^a	2.2 ± 0.3^a
ID3	RT, CD	−2.2 ± 0.3^a	−2.4 ± 0.2^a	−1.9 ± 0.2^a	−2.4 ± 0.3^a
IFNG	IR, CD	589.3 ± 190.5^a, b	25.1 ± 12.0^a, b	22.0 ± 9.2^a, b	2.1 ± 0.8^b
IL1B	IR, CD	1850.1 ± 415.0^a	1353.8 ± 333.5^a	3248.0 ± 1069.1^a	2048.6 ± 611.3^a
IL6	IR, CD	79.5 ± 20.7^a	77.9 ± 31.2^a	171.1 ± 89.3^a	97.7 ± 37.9^a
IL10	IR, CD	69.5 ± 20.4^a	107.3 ± 31.8^a	125.7 ± 44.6^a	112.2 ± 18.6^a
IL12B	IR, CD	17 044.3 ± 16 476.7^a, b	34 218.1 ± 32 873.8^a, b	2591.0 ± 1834.2^a	3340.4 ± 1966.5^a
ISG15	IR	304.8 ± 57.8^a, b	165.7 ± 28.0^a, b	120.0 ± 48.2^a, b	46.8 ± 19.0^a, b
JUNB	RT	15.2 ± 4.1^a, b	7.4 ± 0.9^a, b	12.2 ± 1.0^a, b	8.7 ± 1.0^a, b
KLF5	RT	9.0 ± 0.8^a	9.4 ± 1.3^a	8.7 ± 1.5^a	9.1 ± 1.4^a
KLF13	RT	−2.9 ± 0.5^a	−2.7 ± 0.3^a	−3.1 ± 0.2^a	−2.5 ± 0.3^a
LIG4	RT, CD	2.9 ± 0.3^a	4.0 ± 0.5^a	3.2 ± 0.3^a, b	4.9 ± 0.5^a, b
MEFV	IR	82.0 ± 27.7^a	111.6 ± 15.8^a	76.5 ± 14.3^a, b	140.1 ± 16.2^a, b
MYC	RT, CD	−0.5 ± 0.8^b	−2.3 ± 0.5^a, b	−0.9 ± 0.6	−1.7 ± 0.2^a
NR4A2	RT, CD	4.4 ± 0.8^a, b	1.1 ± 0.5^b	2.6 ± 0.2^a	1.9 ± 0.3^a
PLEKHF1	CD	−5.2 ± 0.7^a	−4.1 ± 0.6^a	−3.8 ± 0.6^a	−3.2 ± 0.3^a
TNF	IR, CD	353.7 ± 104.3^a	368.6 ± 58.0^a	551.6 ± 282.1^a	452.5 ± 171.5^a
TNFAIP6	IR	683.3 ± 301.9^a	927.7 ± 377.9^a	9115.6 ± 5564.9^a	9148.4 ± 6115.0^a
TXNIP	IR, RT, CD	2.2 ± 0.8^a, b	1.8 ± 0.7^b	3.0 ± 0.3^a, b	0.9 ± 0.5^b
YWHAB	RT, CD	−1.3 ± 0.1^a	−0.8 ± 0.4	−0.4 ± 0.5	−1.1 ± 0.0^a

The results are expressed as the average fold change in mRNA levels in DC treated with live (DC_L) and inactivated (DC_D) S. Typhimurium and Mø treated with live (Mø _L) and inactivated (Mø _D) S. Typhimurium compared to the uninfected DC or Mø ± standard errors.
^aIndicates when the mean fold change values were significantly different from resting cells by t test corrected for multiple testing (Benjamini–Hochberg) (P < 0.05).
^bDenotes when the mean fold change measured in DC and/or Mø is significantly different in the response to live and inactivated Salmonella by t test corrected for multiple testing (Benjamini–Hochberg) (P < 0.05). The GO term column indicates which of the three major GO terms the gene is associated with; inflammatory response (IR), regulation of transcription (RT) and regulation of programmed cell death (CD).

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ISSN: 1297-9716

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