Live and inactivated Salmonella enterica serovar Typhimurium stimulate similar but distinct transcriptome profiles in bovine macrophages and dendritic cells

Table 5 Direct comparison of mRNA levels for investigated genes in DC and Mø

Gene symbol	Cell-type effect	Average fold differences between cell types
Gene symbol	Cell-type effect	Unstimulated cells		Live S. Typhimurium		Inactivated S. Typhimurium
CXCL2	P = 0.006	Mø	1.1 ± 1.3	Mø	2.7 ± 0.3^a	Mø	2.0 ± 0.4
ETS1	P = 0.023	Mø	1.6 ± 0.6	Mø	1.1 ± 0.4	Mø	1.4 ± 0.6
ID3	P < 0.001	Mø	1.7 ± 0.4	Mø	1.6 ± 0.1^a	Mø	1.3 ± 0.1
IFNG	n.s.	Mø	3.3 ± 0.9	DC	15.4 ± 6.7^a	DC	4.2 ± 1.3
IL1B	P < 0.001	Mø	3.2 ± 1.9	Mø	4.0 ± 1.0^a	Mø	3.6 ± 0.8^a
IL6	P = 0.019	Mø	8.3 ± 7.4	Mø	7.7 ± 5.5	Mø	8.0 ± 5.0
ISG15	n.s.	Mø	7.5 ± 3.9^a	DC	1.1 ± 0.2	DC	1.5 ± 0.3
KLF13	P < 0.001	DC	1.8 ± 0.2^a	DC	1.1 ± 0.8^a	DC	1.7 ± 0.3^a
NR4A2	P = 0.019	DC	1.2 ± 0.1	DC	2.1 ± 0.3^a	DC	1.0 ± 0.1
TNF	P < 0.001	Mø	2.8 ± 1.7	Mø	4.2 ± 1.8^a	Mø	2.3 ± 0.2
TXNIP	n.s.	DC	0.4 ± 1.4	DC	0.6 ± 0.3	DC	1.2 ± 0.4^a
YWHAB	P = 0.024	Mø	0.2 ± 0.6	Mø	0.1 ± 0.5	Mø	0.4 ± 0.4

The direct comparison the relative mRNA levels in DC and Mø revealed that 12 genes exhibited a cell-type effect by GLM and subsequent Fisher’s test (with Benjamini–Hochberg correction for multiple testing). The cell-type effect column summarizes the statistical significance of the effect of cell-type across all samples by GLM and subsequent Fisher’s test, with Benjamini–Hochberg correction for multiple testing. The average fold difference between the cell types was calculated for each condition (unstimulated, infected with live S. Typhimurium, stimulated with inactivated S. Typhimurium) and the cell type with the highest level of expression is indicated, with the average fold difference ± standard error.
^aIndicates where a significant difference in the average fold difference between DC and Mø was identified for the cell type and condition interaction (Fisher’s test, with Benjamini–Hochberg correction for multiple testing P < 0.05), n.s. denotes when the effect of cell type was not significant.

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