- Open Access
Small ruminant macrophage polarization may play a pivotal role on lentiviral infection
- Helena Crespo†1,
- Luigi Bertolotti†2,
- Magda Juganaru2,
- Idoia Glaria1,
- Damián de Andrés1,
- Beatriz Amorena1,
- Sergio Rosati†2 and
- Ramsés Reina†1Email author
© Crespo et al.; licensee BioMed Central Ltd. 2013
- Received: 28 March 2013
- Accepted: 26 August 2013
- Published: 26 September 2013
Small ruminant lentiviruses (SRLV) infect the monocyte/macrophage lineage inducing a long-lasting infection affecting body condition, production and welfare of sheep and goats all over the world. Macrophages play a pivotal role on the host’s innate and adaptative immune responses against parasites by becoming differentially activated. Macrophage heterogeneity can tentatively be classified into classically differentiated macrophages (M1) through stimulation with IFN-γ displaying an inflammatory profile, or can be alternatively differentiated by stimulation with IL-4/IL-13 into M2 macrophages with homeostatic functions. Since infection by SRLV can modulate macrophage functions we explored here whether ovine and caprine macrophages can be segregated into M1 and M2 populations and whether this differential polarization represents differential susceptibility to SRLV infection. We found that like in human and mouse systems, ovine and caprine macrophages can be differentiated with particular stimuli into M1/M2 subpopulations displaying specific markers. In addition, small ruminant macrophages are plastic since M1 differentiated macrophages can express M2 markers when the stimulus changes from IFN-γ to IL-4. SRLV replication was restricted in M1 macrophages and increased in M2 differentiated macrophages respectively according to viral production. Identification of the infection pathways in macrophage populations may provide new targets for eliciting appropriate immune responses against SRLV infection.
- Small Ruminant
- Mannose Receptor
- Macrophage Polarization
- Pseudotyped Virus
- Entry Assay
Small Ruminant Lentivirus (SRLV) infection is spreading all over the world, with new descriptions in countries such as Poland , Sultanate of Oman, Canada , Slovenia , Russia  and new genotypes such as A12, A14 and A15 , E and B3 [5–7]. Today, highly efficient prophylactic/therapeutic measures against SRLV do not exist, and control is frequently based on early diagnosis and culling of seropositive ewes and their progeny. SRLV infection occurs early after parturition by the lactogenic route or direct contact with infected animals. The main target cells for the virus in vivo are the monocyte/macrophage lineage  whereas CD4 T cells and dendritic cells also play important roles [9, 10]. Upon infection, initial viral replication triggers T cell and antibody responses that control virus burst and allow serological diagnosis. Following this stage, viral infection is partially controlled but provirus is already integrated at low levels into the cellular genome mainly of monocytes, myeloid cells or tissue macrophages . The provirus remains latently integrated until viral proteins are produced and new virions are released. This results in a continuous process of low viral replication together with expression of pro-inflammatory cytokines and chemokines that often lead to a strong inflammatory process, tissue damage and disease development . The permissiveness of macrophages to SRLV in vitro is modulated by cytokines. Specifically, IFN-γ restricts SRLV replication by delaying macrophage maturation [12–14], whereas increased IL-8, GM-CSF, IL-16, IL-1beta, IL-4 and IL-10 have been associated in vivo to seropositivity [15–18] and SRLV replication in vitro.
Cells of the monocyte-macrophage lineage are heterogeneous reflecting the plasticity and versatility of the response to environmental stimuli required for effective immune responses. Different patterns of macrophage maturation have been identified in humans and mice according to differentiation mechanisms and identification of markers for such subpopulations . Macrophage classical (M1 phenotype) and alternative activations (M2 phenotype) depend on the presence of molecules secreted by T-helper CD4 or NK cells . In particular, M1 phenotype is induced by Th1-signature cytokines such as IFN-γ and TNF-α as well as LPS, resulting in enhanced microbicidal ability in addition to increased proinflammatory responses and cellular immunity . In HIV-1 infection, the M1 profile is characterized by down-regulation of CD4 receptors, increased CCR5-binding chemokines and significantly decreased viral production, likely at pre-integration steps . On the contrary, the M2 phenotype is displayed after induction with Th2-hallmark cytokines such as IL-4 and IL-13, which play a major role in responses against parasites, allergy, wound healing, tissue remodeling or in some cases to regulate the immune response .
In contrast with humans and mice, data on macrophage polarization and its effect on lentiviral infection are lacking in other animal species. This study aimed to investigate whether sheep and goats, both targets to SRLV infections, also display macrophage subpopulations. For this, we explored putative stimuli that would elicit such subpopulations and determined cell markers to identify them. With the information obtained, we investigated if the putative subpopulations had different permissiveness and effectiveness of infection by different strains of SRLV, according to performance in an entry assay and retrotranscriptase activity, respectively.
Cells and viruses
Fibroblastic-like cells from skin biopsies were obtained from SRLV-free animals. Cells were grown in DMEM medium supplemented with 10% foetal bovine serum, 2% L-glutamine, 1% antibiotics/antimycotics mix and 1:1000 gentamicin (Sigma-Aldrich, Steinheim, Germany).
Blood monocyte-derived macrophages (MDM) were obtained by isolation of peripheral blood mononuclear cells (PBMC) from SRLV-free sheep and goats in compliance with the relevant National legislation on experimental animals and animal welfare, upon authorization by the competent authority (Italian Ministry of Health-Directorate General Animal Health-Office VI, permit no.07/2009B), by Ficoll (1.077) gradient centrifugation. MDM were grown in RPMI complete medium, containing RPMI 1640 supplemented with 10% foetal goat serum, 10 mM sodium pyruvate, 1% non-essential amino acids, 1% vitamins, 1% antibiotics/antimycotics mix and 1:1000 gentamicin, 1% L-glutamine and 50 μM 2-mercaptoetanol (Sigma-Aldrich).
HEK293-T cells were cultured in DMEM (GIBCO, Invitrogen, Paisley, UK) supplemented with 10% foetal bovine serum, 1% antibiotics/antimycotics and 1:1000 gentamicin.
CHO cells and CHO-MR cells expressing mouse mannose receptor (kindly provided by Dr Luisa Martínez-Pomares; ) were cultured in F12 nutrient mixture with 10% foetal bovine serum, 1% L-glutamine, 1% antibiotics/antimycotics and 1:1000 gentamicin.
Six different SRLV strains were used, one from the genotype A VMV-like Ev1 strain , three of them belonging to the genotype B CAEV-Co  and CAEV-To1/89 (B1, ), and 496 (B2, ) and two belonging to the genotype E, Roccaverano (E1,  and Seui (E2, ).
Different small ruminant cytokines were used as well as LPS (Sigma) as stimulators for macrophage polarization. Plasmid pN3-IFN-γ containing the ovine IFN-γ gene was kindly provided by Dr Marie Suzan-Monti (Faculté de Médecine, Marseille, France).
Primers used for amplification and cloning of the cytokines and the Caev-Cork env gene used in entry assays.
AATTCTCGAG ACCATGG GTCTCACCTCCCAGC
AATTCTCGAG ACCATGG CGCTCTTCTTGAC
AATTCTCGAG ACCATG CCCAGCAGCTCAG
128 nt 
91 nt 
Polymerase chain reactions (PCR) were performed in a final volume of 50 μL with 3 μL of the cDNA samples. The reaction mixture contained 1× PCR buffer, 2 μM dNTP, 3 nM of each primer and 1.25 units of Hot Start Polymerase (Qiagen, Hilden, Germany). The PCR started with a 95 °C step (15 min), followed by a denaturation step at 94 °C for 30 s, 1 min of annealing at 55 °C and a 1 min extension at 72 °C. A final extension of 72 °C for 10 min was also carried out.
PCR products were submitted to electrophoresis in a 1.5% agarose gel, and the selected amplicons were purified by Qiagen PCR clean-up kit, digested with XhoI/Acc561 restriction enzymes and cloned into the previously digested expression vector pN3. Chemically TOP10 competent cells (Invitrogen, Paisley, UK) were transformed and grown overnight in LB agar plates supplemented with kanamycin (50 ng/mL). Colonies were screened by PCR with specific primers for the vector sequences, run in 1.5% agarose gel and positive clones were selected and sequenced (Secugen, Madrid, Spain).
Plasmids containing the correct IL-4, IL-13 or IL-10 sequence were employed for transfection of cultured HEK 293-T cells using the Amaxa Nucleofector II device and following the manufacturer’s protocol from Cell Line Nucleofector Kit V (LONZA, Köln, Germany). After 72 h, cell supernatants were collected, clarified by centrifugation and frozen at −80 °C. HEK 293-T cells were also transfected with the empty plasmid pN3 and the supernatant was used as a non-stimulated control.
Quantification of cytokines for use as stimulators in macrophage polarization
IFN-γ and IL-4 cytokine concentrations and biological activities in HEK 293-T culture supernatants was measured with the Ovine IFN-γ ELISA kit (Mabtech, Nacka Strand, Sweden) and the Bovine IL-4 screening set (Thermo Scientific, Rockford, USA), respectively, according to the manufacturers’ protocols. The concentration obtained was similar in both cases, ranging from 600 to 2600 ng and was adjusted to 50 ng/mL for use in MDM stimulations (see below).
In the absence of commercially available quantification kits for small ruminant IL-10 and IL-13, the concentration range of these cytokines in the HEK 293-T culture system was assumed to be the same as for IFN-γ and IL-4. Nevertheless, biological activity was first verified by addition of 5, 10 and 20-fold dilutions of the HEK 293-T culture supernatants to MDM, followed by a 4 h incubation, cell harvesting, RNA extraction and determination of marker expression by real time RT-PCR (see below).
LPS and cytokines (IFN-γ, IL-4, IL-13 and IL-10) were added to macrophages on day 3 of culture, at a final concentration of 50 ng/mL. Supernatants from HEK 293-T cultures transfected with empty pN3 plasmid were used as the negative control. Fresh medium supplemented with cytokines was partially replaced after 3–4 days of culture. On day 6, stimulated MDM were either collected for RNA extraction and marker molecule expression analysis (at least six independent wells for each treatment), or infected for RT activity measurements or used in entry assay experiments, as indicated below.
Marker expression analysis by real time RT-PCR
RNA was extracted from cytokine treated and pN3-treated MDM and real time RT-PCR were carried out using specific primers and probes according to the differentiation pathway, TNF-α, CD80 and APOBEC3Z1 (A3Z1) for M1 phenotype and Mannose Receptor (MR), DC-SIGN and IL-10 for M2 phenotype (Table 1). TNF-α, CD80 and IL-10 real time PCR procedures were performed using specific probes and conditions described previously for CD80  A3Z1, MR and DC-SIGN expression was assessed with specific primers and SybrGreen Master Mix (Takara, Otsu, Japan) following conditions described previously .
β-actin expression was measured as a housekeeping gene, thus β-actin Ct values were subtracted from the markers’ Ct values for each sample, in order to obtain the 2-ΔCt values used for comparisons.
Viral infections and RT activity determination
Six-day stimulated MDM were infected at 0.1 TCID50/cell with one of the six SRLV strains under study, being one of genotype A (Ev1), three of genotype B (CAEV-Co, CAEV-To and 496) and two of the recently described genotype E of SRLV (Roccaverano and Seui). Following 2-h incubation, the cells were washed three times with PBS and fresh medium containing the stimulating cytokines was added. Supernatants were collected at 7 days post inoculation for Retrotranscriptase activity (RT activity) quantification, performed with the HS-Lenti RT activity kit (Cavidi, Uppsala, Sweden) according to the manufacturer’s instructions.
Viral pseudotype constructions and entry assay
Plasmids containing the envelope gene (env) of Roccaverano, Seui  and Ev1 strains  were used. In addition, CAEV-Co env was cloned into the pCMV plasmid  using the same strategy and with the primer pairs shown in Table 1. Positive clones were screened by restriction enzyme digestion, sequencing and by assessing the formation of syncitya in cultured skin fibroblasts. pMDG plasmid encoding for vesicular stomatitis virus env glycoprotein (VSV-G) was used as a positive control (kindly provided by Prof. Greg J. Towers, University of London, UK).
For entry assays, pseudotyped virions were produced by co-transfection of HEK 293-T cells with two types of plasmids as described . Briefly, pCAEV-AP (encoding alkaline phosphatase), kindly provided by Dr Isidro Hötzel, ; and one of the env-containing plasmid constructs described above. HEK 293-T cell culture supernatants containing the pseudotyped viruses were collected after 48 h, clarified and used in 10-fold dilutions to infect MDM, CHO and CHO-MR cells. After 48 h, the cells were stained using the alkaline phosphate substrate BCIP/NBT (Thermo Scientific)  and the results were expressed as focus-forming unit per mL (FFU/mL).
Stimulated and pN3-stimulated MDM, CHO and CHO-MR cells were treated with 1 mg/mL of mannan (Sigma-Aldrich), for 30 min at 37 °C and 5% CO2, and infected with 10-fold dilutions of the pseudotyped viruses plus mannan following the entry assay procedure described above. Cells non-treated with mannan were included as controls.
MDM were allowed to differentiate in complete medium without cytokine stimulation and then infected with 0.1 TCID50/mL of the six strains mentioned above (EV1, CAEV-Co, CAEV-To, 496, Roccaverano and Seui). On day 7 of infection, the cells were harvested and RNA were extracted and retrotranscribed to cDNA. Real time PCR for the detection of TNF-α, CD80, A3Z1, MR, DC-SIGN and IL-10, were carried out as described above.
MDM were obtained and allowed to differentiate into M1 or M2 profiles with IFN-γ and IL-4 respectively through two cycles of stimulation (3 days each). After the second, MDM were washed and stimulated for 3 additional days with a cytokine of the opposite phenotype (M1 vs. M2), then RNA was obtained and cDNA were subjected to specific amplification of differentiation pathway markers.
The effects of different stimulators on marker expression were tested evaluating correlation between 2-ΔCt and stimulator concentration with the Spearman’s rank correlation test. In order to evaluate the possible differences in expression among cells treated with different stimuli or non-treated (i.e. control pN3 group), data were analysed using Kruskal Wallis test. If a significant difference was recorded, a planned comparison between each treated cell group and the control was performed using 2-tailed Wilcoxon rank-sum test or Mann-Whitney’s test. Correlations and differences were considered significant if associated p < 0.05.
Cytokine biological activity
Therefore these cytokines, in addition to those whose activity had been assessed by commercial kits (IFN-γ and IL-4), were considered biologically active and suitable for further MDM stimulation studies.
Characterization and plasticity of polarized macrophages
Phenotypic differences regarding cell morphology (shape and size) were clearly observed between differentially stimulated MDM after 3 days of cytokine stimulation. Specifically, IFN-γ and LPS-treated (Th1 cytokines) cells were small and round, whereas IL-4 treated MDM showed a dendritic-like shape with large pseudopodia. Finally, in macrophages exposed to IL-13, IL-10 and pN3 there appeared to be a mixture of morphological phenotypes.
Additional molecules evaluated in this study were identified as new markers for M1 or M2 macrophage populations. A3Z1 and CD80 were induced in IFN-γ stimulated MDM, identifying M1 macrophages. Conversely, DC-SIGN was highly expressed in IL-4 and IL-13 stimulated macrophages, identifying M2 macrophages (Figure 2).
IL-10 expression values did not seem to distinguish between different populations (p > 0.05 in all comparisons), although expression was mainly observed in IL-4, IL-10 and LPS stimulated macrophages.
Apart from these clear patterns of M1 vs. M2 polarization, “single-effect” patterns associated to caprine or ovine species were also identified. Specifically, LPS stimulation resulted in highly increased levels of MR in goats and IL-10 in sheep and not in increased M1 cytokines, whereas IL-13 induced high levels of MR and DC-SIGN in both species. CD80 was a good marker for M1 macrophages in the caprine species whereas IL-4 stimulation led to expression of IL-10 only in sheep.
pN3 induced the lowest levels of stimulation in ovine and caprine macrophages. As in other animal models, pN3 (control) exposure resulted in increased levels of M2 marker expression.
Susceptibility of MDM stimulated with cytokines to virus infection
SRLV infection promotes the M2 differentiation pathway
In contrast, TNFα, CD80 and A3Z1 and IL-10 expression did not increase upon infection with most of the strains. Remarkably, infection with Seui strain induced higher TNFα, CD80 and IL-10 expression than pN3 treated cells (Figure 5).
Env-mediated entry studies with pseudotyped viruses and MR involvement
Viral entry was significantly inhibited by mannan treatment when using Roccaverano pseudotyped particles overall in IL-4 stimulated MDM, and the inhibitory effect was non-significant for Ev1, CAEV-Co, Seui or VSV-G pseudotyped virions. The highest entry values were reached with pantropic VSV-G pseudovirions, as expected (Figure 6a).
Next, we determined if the mannan blocking effects of env-mediated entry and subsequent infection could be attributed to the involvement of the MR receptor at viral entry, also using for this the pseudoviruses described above. For this we used CHO-MR cells stably expressing MR , but not other SRLV receptors, and assessed the effect of mannan treatment (Figure 6b). CHO-MR cells challenged with EV1, CAEV-Co, Roccaverano, Seui or VSV-G pseudotyped viruses showed a heterogeneous pattern of viral entry and suggest that mannan exposure would inhibit infections by Ev1, Roccaverano and Seui pseudotyped virions but did not hamper CAEV-Co or VSV-G entry (Figure 6b).
In SRLV infections, immune responses constitute a double-edge sword, since on one side they are essential against infections  and on the other side they lead to follicular hyperplasia and mononuclear cell infiltration, the main causes of tissue damage in SRLV-related lesions [11, 38]. Macrophages, the main target cells for SRLV infection, play a pivotal role in the process, disseminating the virus and providing antigen, costimulatory signals and cytokines, inducers or regulators of immune responses. In addition, macrophages suffer alterations upon lentiviral infection, but subsets of macrophages have not been identified yet in small ruminant species. Our results, strongly suggest that M1 and M2, main patterns of differentiation, are well conserved among species - including small ruminants - being induced by IFN-γ and IL-4, respectively. In small ruminants, stimulation with M1-like stimulus (IFN-γ) clearly induced an increased expression of not only TNF-α, as in humans and mice  but also APOBEC3 (A3Z1) and the costimulatory molecule CD80, together with a decreased expression of MR and DC-SIGN. Conversely, stimulation with M2 stimulus (IL-4, IL-13, IL-10) conferred an induction of not only MR but also DC-SIGN and IL-10. Interestingly, LPS and negative controls (pN3) induced an intermediate (M1-to-M2) pattern and a clear M2 pattern, respectively. LPS has been associated with an M2b profile , with high levels of proinflammatory cytokines together with high IL-10 [40, 41] as shown by our studies.
SRLV viral infection induced M2 polarization of macrophages, which also exhibited high MR and DC-SIGN expression as observed upon IL-4 stimulation. If this SRLV-mediated M2 induction occurs in vivo in alveolar macrophages of infected animals, infection would be enhanced, as these cells would show a M2 phenotype with a high expression of pattern-recognition receptors such as MR and scavenger receptors . This pattern would favor viral spread as observed  and also in this study using M2 polarized macrophages. Ovine alveolar macrophages expressing scavenger receptor CD163, a hallmark of M2 differentiation, were found to be highly related to the presence of SRLV capsid protein in persistently infected sheep , in agreement with our results. However, during early infection in vivo, new M1 cells may infiltrate into the tissue and initially control the incoming virus. After encountering the virus (Figure 5) and also for physiological reasons (M1 cells last a few days; ), these M1 would eventually switch to M2 macrophages  allowing virus replication as well as infection spread since M2 cells can live from several weeks to years . M1 cells are non-motile since inflammation is better tolerated in a restricted site, but M2 cells instead are motile (amoeboid and mesenchymal modes), able to move across a 3D matrix  favoring infection spread to other target organs. M2 cells are tolerogenic and surely are the main macrophages in SRLV infection since animals that reach the clinical stage present high levels of IgG1, IL-4 and low levels of B7 molecule transcripts that would impair antigenic presentation leading to anergic responses and T cell exhaustion. However, care should be taken when interpreting this picture since it is also plausible that M1 cells somehow impaired in their ability to restrict SRLV, could be responsible for both the tolerance and immunomediated lesions.
The Roccaverano SRLV strain showed an increased entry level into M2 macrophages compared to M1 suggesting a different receptor usage for genotype E1, whereby this genotype would enter the cell mainly via mannose recognizing receptors such as MR, whereas other strains would use additional receptors present in macrophages [31, 32]. The levels of env-related entry into CHO-MR cells found in this study in the presence/absence of mannan may also suggest that SRLV can enter M2 cells via MR.
M2, the most susceptible macrophages for SRLV infection, could allow viral proliferation and promote pathogenesis in vivo by at least two different mechanisms. One pathway would involve cellular receptors that may allow or enhance viral entry, and the other related to poor expression of B7 (CD80/86) costimulatory molecules, also observed here in M2 profiles, which would lead to the lack of T cell responses in vivo . Arguing also in favour of these mechanisms, are studies showing that IFN-γ (leading to the M1 phenotype) selectively down regulates activity of MR [49, 50] and M1 chemokines amplify the DTH response, which is reduced in SRLV affected animals .
Interestingly, infection block (decreased RT-activity) in M1 compared to M2 MDM did not occur at the entry step, since the degree of entry was similar in M1 and M2 cells. Rather, blockage occurred at post-entry steps since preliminary qPCR data suggest that integration, evaluated as proviral load, was lower in IFN-γ than IL-4 stimulated cells (data not shown). Post-integration restriction may include the involvement of TRIM5 and APOBEC, as described in human HIV-1 infections . M1 and M2 human macrophages showed restriction against HIV-1 infection by inducing factors like APOBEC3G or reducing the expression of certain receptors (CD4) respectively . In this study, M1 differentiation may have restricted SRLV replication through A3Z1 induction, according to the A3Z1 mRNA levels observed. Conversely, M2 favoured virus replication likely due to differential receptor usage of SRLV compared with HIV-1.
Macrophages respond to stimuli temporarily reverting their differentiation pattern, depending on the cytokine present [52, 53]. Thus polarization is a continuous reversible M1/M2 differentiation process, as confirmed in ovine macrophages, reflecting that profiles are not static terminally-differentiated states. This plasticity may have important consequences in chronic lentiviral infections where SRLV replicates at higher levels in M2 compared to M1 macrophages. Different strategies can be proposed in order to block or at least relieve M2 differentiation and therefore viral spread, since macrophages do not necessarily suffer apoptosis or emigration after inflammation [54, 55]. These strategies have been applied against tumour diseases, where an M2 phenotype of macrophages may predominate  and the cure has involved a switch of the immune response to M1 by pharmacological treatment .
Interestingly, macrophages previously exposed to rhinoviruses show a reduced antibacterial capacity  and also SRLV infected macrophages showed a reduced phagocytic activity against bacteria indirectly facilitating secondary infections  suggesting the induction of an M2 phenotype. Mycoplasma co-infection malp (macrophage activation lipoprotein) may modify proteins presented on the plasmatic membrane that could act as SRLV (co)-receptors and favour infection . Essentially, data regarding macrophage differentiation and characterization of the respective profiles share several features with the well depicted picture in humans and mice strongly suggesting a widely conserved maturation pathway, further reinforcing the M1/M2 model and validating their use.
This work was funded by the Italian Ministry of Instruction, University and Research PRIN 2008 and Piedmont Region “Ricerca Sanitaria Finalizzata” 2008; and by grants from CICYT (no. AGL2010-22341-C04-01), and the Government of Navarra (no. IIQ14064.RI1). We acknowledge the Public University of Navarra and CSIC for fellowships and the JAE-contract (HC and RR).
- Olech M, Rachid A, Croise B, Kuzmak J, Valas S: Genetic and antigenic characterization of small ruminant lentiviruses circulating in Poland. Virus Res. 2011, 163: 528-536.View ArticlePubMedGoogle Scholar
- L’Homme Y, Ouardani M, Levesque V, Bertoni G, Simard C, Pisoni G: Molecular characterization and phylogenetic analysis of small ruminant lentiviruses isolated from Canadian sheep and goats. Virol J. 2011, 8: 271-10.1186/1743-422X-8-271.PubMed CentralView ArticlePubMedGoogle Scholar
- Kuhar U, Barlic-Maganja D, Grom J: Phylogenetic analysis of small ruminant lentiviruses detected in Slovenia. Vet Microbiol. 2013, 162: 201-206. 10.1016/j.vetmic.2012.08.024.View ArticlePubMedGoogle Scholar
- Baryshnikova EI, Malogolovkin AS, Kolbasova OL, Tsybanov S: Comparative characteristics of the biological properties of small ruminant lentiviruses. Vopr Virusol. 2011, 56: 42-45. (in Russian)PubMedGoogle Scholar
- Grego E, Bertolotti L, Quasso A, Profiti M, Lacerenza D, Muz D, Rosati S: Genetic characterization of small ruminant lentivirus in Italian mixed flocks: evidence for a novel genotype circulating in a local goat population. J Gen Virol. 2007, 88: 3423-3427. 10.1099/vir.0.83292-0.View ArticlePubMedGoogle Scholar
- Reina R, Bertolotti L, Dei Giudici S, Puggioni G, Ponti N, Profiti M, Patta C, Rosati S: Small ruminant lentivirus genotype E is widespread in Sarda goat. Vet Microbiol. 2010, 144: 24-31. 10.1016/j.vetmic.2009.12.020.View ArticlePubMedGoogle Scholar
- Bertolotti L, Mazzei M, Puggioni G, Carrozza ML, Dei Giudici S, Muz D, Juganaru M, Patta C, Tolari F, Rosati S: Characterization of new small ruminant lentivirus subtype B3 suggests animal trade within the Mediterranean Basin. J Gen Virol. 2011, 92: 1923-1929. 10.1099/vir.0.032334-0.View ArticlePubMedGoogle Scholar
- Narayan O, Kennedy-Stoskopf S, Sheffer D, Griffin DE, Clements JE: Activation of caprine arthritis-encephalitis virus expression during maturation of monocytes to macrophages. Infect Immun. 1983, 41: 67-73.PubMed CentralPubMedGoogle Scholar
- Ryan S, Tiley L, McConnell I, Blacklaws B: Infection of dendritic cells by the Maedi-Visna lentivirus. J Virol. 2000, 74: 10096-10103. 10.1128/JVI.74.21.10096-10103.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Eriksson K, McInnes E, Ryan S, Tonks P, McConnell I, Blacklaws B: CD4(+) T-cells are required for the establishment of maedi-visna virus infection in macrophages but not dendritic cells in vivo. Virology. 1999, 258: 355-364. 10.1006/viro.1999.9711.View ArticlePubMedGoogle Scholar
- Blacklaws BA: Small ruminant lentiviruses: immunopathogenesis of visna-maedi and caprine arthritis and encephalitis virus. Comp Immunol Microbiol Infect Dis. 2012, 35: 259-269. 10.1016/j.cimid.2011.12.003.View ArticlePubMedGoogle Scholar
- Kennedy PG, Narayan O, Ghotbi Z, Hopkins J, Gendelman HE, Clements JE: Persistent expression of Ia antigen and viral genome in visna-maedi virus-induced inflammatory cells. Possible role of lentivirus-induced interferon. J Exp Med. 1985, 162: 1970-1982. 10.1084/jem.162.6.1970.View ArticlePubMedGoogle Scholar
- Juste RA, Ott TL, Kwang J, Bazer FW, de La Concha-Bermejillo A: Effects of recombinant ovine interferon-tau on ovine lentivirus replication and progression of disease. J Gen Virol. 2000, 81: 525-532.View ArticlePubMedGoogle Scholar
- Zink MC, Narayan O, Kennedy PG, Clements JE: Pathogenesis of visna/maedi and caprine arthritis-encephalitis: new leads on the mechanism of restricted virus replication and persistent inflammation. Vet Immunol Immunopathol. 1987, 15: 167-180. 10.1016/0165-2427(87)90110-3.View ArticlePubMedGoogle Scholar
- Legastelois I, Cordier G, Cozon G, Cadore JL, Guiguen F, Greenland T, Mornex JF: Visna-maedi virus-induced expression of interleukin-8 gene in sheep alveolar cells following experimental in vitro and in vivo infection. Res Virol. 1996, 147: 191-197. 10.1016/0923-2516(96)80234-2.View ArticlePubMedGoogle Scholar
- Zhang Z, Harkiss GD, Hopkins J, Woodall CJ: Granulocyte macrophage colony stimulating factor is elevated in alveolar macrophages from sheep naturally infected with maedi-visna virus and stimulates maedi-visna virus replication in macrophages in vitro. Clin Exp Immunol. 2002, 129: 240-246. 10.1046/j.1365-2249.2002.01826.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Sharmila C, Williams JW, Reddy PG: Effect of caprine arthritis-encephalitis virus infection on expression of interleukin-16 in goats. Am J Vet Res. 2002, 63: 1418-1422. 10.2460/ajvr.2002.63.1418.View ArticlePubMedGoogle Scholar
- Woodall CJ, Maclaren LJ, Watt NJ: Differential levels of mRNAs for cytokines, the interleukin-2 receptor and class II DR/DQ genes in ovine interstitial pneumonia induced by maedi visna virus infection. Vet Pathol. 1997, 34: 204-211. 10.1177/030098589703400305.View ArticlePubMedGoogle Scholar
- Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, Locati M: The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol. 2004, 25: 677-686. 10.1016/j.it.2004.09.015.View ArticlePubMedGoogle Scholar
- Vacca P, Cantoni C, Vitale M, Prato C, Canegallo F, Fenoglio D, Ragni N, Moretta L, Mingari MC: Crosstalk between decidual NK and CD14+ myelomonocytic cells results in induction of Tregs and immunosuppression. Proc Natl Acad Sci U S A. 2010, 107: 11918-11923. 10.1073/pnas.1001749107.PubMed CentralView ArticlePubMedGoogle Scholar
- Gordon S: The macrophage: past, present and future. Eur J Immunol. 2007, 37 (Suppl 1): S9-S17.View ArticlePubMedGoogle Scholar
- Cassol E, Cassetta L, Rizzi C, Alfano M, Poli G: M1 and M2a polarization of human monocyte-derived macrophages inhibits HIV-1 replication by distinct mechanisms. J Immunol. 2009, 182: 6237-6246. 10.4049/jimmunol.0803447.View ArticlePubMedGoogle Scholar
- Mantovani A, Biswas SK, Galdiero MR, Sica A, Locati M: Macrophage plasticity and polarization in tissue repair and remodelling. J Pathol. 2013, 229: 176-185. 10.1002/path.4133.View ArticlePubMedGoogle Scholar
- Martinez-Pomares L, Reid DM, Brown GD, Taylor PR, Stillion RJ, Linehan SA, Zamze S, Gordon S, Wong SY: Analysis of mannose receptor regulation by IL-4, IL-10, and proteolytic processing using novel monoclonal antibodies. J Leukoc Biol. 2003, 73: 604-613. 10.1189/jlb.0902450.View ArticlePubMedGoogle Scholar
- Sargan DR, Bennet ID, Cousens C, Roy DJ, Blacklaws BA, Dalziel RG, Watt NJ, McConnell I: Nucleotide sequence of EV1, a British isolate of maedi-visna virus. J Gen Virol. 1991, 72: 1893-1903. 10.1099/0022-1317-72-8-1893.View ArticlePubMedGoogle Scholar
- Saltarelli M, Querat G, Konings DA, Vigne R, Clements JE: Nucleotide sequence and transcriptional analysis of molecular clones of CAEV which generate infectious virus. Virology. 1990, 179: 347-364. 10.1016/0042-6822(90)90303-9.View ArticlePubMedGoogle Scholar
- Grego E, Profiti M, Giammarioli M, Giannino L, Rutili D, Woodall C, Rosati S: Genetic heterogeneity of small ruminant lentiviruses involves immunodominant epitope of capsid antigen and affects sensitivity of single-strain-based immunoassay. Clin Diagn Lab Immunol. 2002, 9: 828-832.PubMed CentralPubMedGoogle Scholar
- Glaria I, Reina R, Crespo H, de Andrés X, Ramirez H, Biescas E, Pérez MM, Badiola J, Lujan L, Amorena B, de Andrés D: Phylogenetic analysis of SRLV sequences from an arthritic sheep outbreak demonstrates the introduction of CAEV-like viruses among Spanish sheep. Vet Microbiol. 2009, 138: 156-162. 10.1016/j.vetmic.2009.03.002.View ArticlePubMedGoogle Scholar
- Reina R, Barbezange C, Niesalla H, de Andrés X, Arnarson H, Biescas E, Mazzei M, Fraisier C, McNeilly TN, Liu C, Perez M, Carrozza ML, Bandecchi P, Solano C, Crespo H, Glaria I, Huard C, Shaw DJ, de Blas I, de Andrés D, Tolari F, Rosati S, Suzan-Monti M, Andrésdottir V, Torsteinsdottir S, Petursson G, Lujan L, Pepin M, Amorena B, Blacklaws B: Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes. Vaccine. 2008, 26: 4494-4505. 10.1016/j.vaccine.2008.06.065.View ArticlePubMedGoogle Scholar
- Reina R, Glaria I, Benavides J, de Andrés X, Crespo H, Solano C, Pérez V, Lujan L, Pérez MM, de la Lastra JM P, Rosati S, Blacklaws B, Harkiss G, de Andrés D, Amorena B: Association of CD80 and CD86 expression levels with disease status of Visna/Maedi virus infected sheep. Viral Immunol. 2007, 20: 609-622. 10.1089/vim.2007.0071.View ArticlePubMedGoogle Scholar
- Crespo H, Reina R, Glaria I, Ramirez H, de Andrés X, Jauregui P, Lujan L, Martinez-Pomares L, Amorena B, de Andrés DF: Identification of the ovine mannose receptor and its possible role in Visna/Maedi virus infection. Vet Res. 2011, 42: 28-10.1186/1297-9716-42-28.PubMed CentralView ArticlePubMedGoogle Scholar
- Juganaru M, Reina R, Bertolotti L, Stella MC, Profiti M, Armentano M, Bollo E, Amorena B, Rosati S: In vitro properties of small ruminant lentivirus genotype E. Virology. 2011, 410: 88-95. 10.1016/j.virol.2010.10.031.View ArticlePubMedGoogle Scholar
- Sanchez AB, Rodriguez D, Garzon A, Amorena B, Esteban M, Rodriguez JR: Visna/maedi virus Env protein expressed by a vaccinia virus recombinant induces cell-to-cell fusion in cells of different origins in the apparent absence of Env cleavage: role of glycosylation and of proteoglycans. Arch Virol. 2002, 147: 2377-2392. 10.1007/s00705-002-0874-7.View ArticlePubMedGoogle Scholar
- Hotzel I, Cheevers WP: Host range of small-ruminant lentivirus cytopathic variants determined with a selectable caprine arthritis- encephalitis virus pseudotype system. J Virol. 2001, 75: 7384-7391. 10.1128/JVI.75.16.7384-7391.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Hotzel I, Cheevers W: Differential receptor usage of small ruminant lentiviruses in ovine and caprine cells: host range but not cytopathic phenotype is determined by receptor usage. Virology. 2002, 301: 21-31. 10.1006/viro.2002.1575.View ArticlePubMedGoogle Scholar
- Miller’s Lab:http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=3479,
- Appay V, Sauce D: Immune activation and inflammation in HIV-1 infection: causes and consequences. J Pathol. 2008, 214: 231-241. 10.1002/path.2276.View ArticlePubMedGoogle Scholar
- Bertoni G, Blacklaws B: Small ruminant lentiviruses and cross species transmission. Lentiviruses and Macrophages: Molecular and Cellular interactions. Edited by: Desport ME. 2010, Norfolk, UK: Caister Academic Press, 277-306.Google Scholar
- Martinez FO, Gordon S, Locati M, Mantovani A: Transcriptional profiling of the human monocyte-to-macrophage differentiation and polarization: new molecules and patterns of gene expression. J Immunol. 2006, 177: 7303-7311.View ArticlePubMedGoogle Scholar
- Mosser DM: The many faces of macrophage activation. J Leukoc Biol. 2003, 73: 209-212. 10.1189/jlb.0602325.View ArticlePubMedGoogle Scholar
- Sica A, Mantovani A: Macrophage plasticity and polarization: in vivo veritas. J Clin Invest. 2012, 122: 787-795. 10.1172/JCI59643.PubMed CentralView ArticlePubMedGoogle Scholar
- Gordon S, Martinez FO: Alternative activation of macrophages: mechanism and functions. Immunity. 2010, 32: 593-604. 10.1016/j.immuni.2010.05.007.View ArticlePubMedGoogle Scholar
- Crespo H, Jauregui P, Glaria I, Sanjose L, Polledo L, Garcia-Marin JF, Lujan L, de Andrés D, Amorena B, Reina R: Mannose receptor may be involved in small ruminant lentivirus pathogenesis. Vet Res. 2012, 43: 43-10.1186/1297-9716-43-43.PubMed CentralView ArticlePubMedGoogle Scholar
- Herrmann-Hoesing LM, Noh SM, Snekvik KR, White SN, Schneider DA, Truscott T, Knowles DP: Ovine progressive pneumonia virus capsid antigen as found in CD163- and CD172a-positive alveolar macrophages of persistently infected sheep. Vet Pathol. 2010, 47: 518-528. 10.1177/0300985809359605.View ArticlePubMedGoogle Scholar
- Bellingan GJ, Caldwell H, Howie SE, Dransfield I, Haslett C: In vivo fate of the inflammatory macrophage during the resolution of inflammation: inflammatory macrophages do not die locally, but emigrate to the draining lymph nodes. J Immunol. 1996, 157: 2577-2585.PubMedGoogle Scholar
- Obot CJ, Morandi MT, Beebe TP, Hamilton RF, Holian A: Surface components of airborne particulate matter induce macrophage apoptosis through scavenger receptors. Toxicol Appl Pharmacol. 2002, 184: 98-106. 10.1006/taap.2002.9493.View ArticlePubMedGoogle Scholar
- Murphy J, Summer R, Wilson AA, Kotton DN, Fine A: The prolonged life-span of alveolar macrophages. Am J Respir Cell Mol Biol. 2008, 38: 380-385. 10.1165/rcmb.2007-0224RC.PubMed CentralView ArticlePubMedGoogle Scholar
- Bozinovski S, Jones JE, Vlahos R, Hamilton JA, Anderson GP: Granulocyte/macrophage-colony-stimulating factor (GM-CSF) regulates lung innate immunity to lipopolysaccharide through Akt/Erk activation of NFkappa B and AP-1 in vivo. J Biol Chem. 2002, 277: 42808-42814. 10.1074/jbc.M207840200.View ArticlePubMedGoogle Scholar
- Whyte CS, Bishop ET, Ruckerl D, Gaspar-Pereira S, Barker RN, Allen JE, Rees AJ, Wilson HM: Suppressor of cytokine signaling (SOCS)1 is a key determinant of differential macrophage activation and function. J Leukoc Biol. 2011, 90: 845-854. 10.1189/jlb.1110644.View ArticlePubMedGoogle Scholar
- Liu Y, Stewart KN, Bishop E, Marek CJ, Kluth DC, Rees AJ, Wilson HM: Unique expression of suppressor of cytokine signaling 3 is essential for classical macrophage activation in rodents in vitro and in vivo. J Immunol. 2008, 180: 6270-6278.View ArticlePubMedGoogle Scholar
- Pyrah IT, Watt NJ: Immunohistological study of the depressed cutaneous DTH response in sheep naturally infected with an ovine lentivirus (Maedi-Visna virus). Clin Exp Immunol. 1996, 104: 32-36. 10.1046/j.1365-2249.1996.d01-661.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Duluc D, Corvaisier M, Blanchard S, Catala L, Descamps P, Gamelin E, Ponsoda S, Delneste Y, Hebbar M, Jeannin P: Interferon-gamma reverses the immunosuppressive and protumoral properties and prevents the generation of human tumor-associated macrophages. Int J Cancer. 2009, 125: 367-373. 10.1002/ijc.24401.View ArticlePubMedGoogle Scholar
- Stout RD, Jiang C, Matta B, Tietzel I, Watkins SK, Suttles J: Macrophages sequentially change their functional phenotype in response to changes in microenvironmental influences. J Immunol. 2005, 175: 342-349.View ArticlePubMedGoogle Scholar
- Stout RD, Suttles J: Immunosenescence and macrophage functional plasticity: dysregulation of macrophage function by age-associated microenvironmental changes. Immunol Rev. 2005, 205: 60-71. 10.1111/j.0105-2896.2005.00260.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Issazadeh S, Navikas V, Schaub M, Sayegh M, Khoury S: Kinetics of expression of costimulatory molecules and their ligands in murine relapsing experimental autoimmune encephalomyelitis in vivo. J Immunol. 1998, 161: 1104-1112.PubMedGoogle Scholar
- Niino D, Komohara Y, Murayama T, Aoki R, Kimura Y, Hashikawa K, Kiyasu J, Takeuchi M, Suefuji N, Sugita Y, Takeya M, Ohshima K: Ratio of M2 macrophage expression is closely associated with poor prognosis for Angioimmunoblastic T-cell lymphoma (AITL). Pathol Int. 2010, 60: 278-283. 10.1111/j.1440-1827.2010.02514.x.View ArticlePubMedGoogle Scholar
- Guiducci C, Vicari AP, Sangaletti S, Trinchieri G, Colombo MP: Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005, 65: 3437-3446.PubMedGoogle Scholar
- Oliver BG, Lim S, Wark P, Laza-Stanca V, King N, Black JL, Burgess JK, Roth M, Johnston SL: Rhinovirus exposure impairs immune responses to bacterial products in human alveolar macrophages. Thorax. 2008, 63: 519-525. 10.1136/thx.2007.081752.View ArticlePubMedGoogle Scholar
- Monleon E, Pacheco MC, Lujan L, Bolea R, Luco DF, Vargas MA, Alabart JL, Badiola JJ, Amorena B: Effect of in vitro maedi-visna virus infection on adherence and phagocytosis of staphylococci by ovine cells. Vet Microbiol. 1997, 57: 13-28. 10.1016/S0378-1135(97)00080-1.View ArticlePubMedGoogle Scholar
- Rosati S, Pozzi S, Robino P, Montinaro B, Conti A, Fadda M, Pittau M: P48 major surface antigen of Mycoplasma agalactiae is homologous to a malp product of Mycoplasma fermentans and belongs to a selected family of bacterial lipoproteins. Infect Immun. 1999, 67: 6213-6216.PubMed CentralPubMedGoogle Scholar
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