Figure 3
From: Small ruminant macrophage polarization may play a pivotal role on lentiviral infection
Macrophage plasticity. MDM were stimulated for a total of 9 days, 6 days with IFN-γ, IL-4 or pN3 (non-stimulated control) followed by a 3-day stimulation with the opposite cytokine as indicated with arrows. Relative expression of the markers TNF-α (a), CD80 (b), A3Z1 (c), MR (d), DC-SIGN (e), and IL-10 (f) was quantified by real time RT-PCR after 6 days of total stimulation. The values are expressed as the median (± interquartile range) 2-ΔCt × 100 value related to β-actin, and represent at least 3 independent experiments. *p < 0.05 (paired Wilcoxon Rank Sum test).