Figure 6
From: Small ruminant macrophage polarization may play a pivotal role on lentiviral infection
Entry assay with pseudotyped viral particles. IFN-γ, IL-4 and pN3 (control) stimulated MDM (a) and CHO-MR (b) were either treated or non-treated with mannan (1 mg/mL) before infection with pseudoviral particles bearing the envelope glycoproteins of the strains EV1 (empty boxes), CAEV-Cork (grey boxes), Roccaverano (horizontal-lined boxes), Seui (vertical-lined boxes) or the control VSV-G (full boxes). Foci of transduced cells were counted using alkaline phosphatase activity staining. Values are expressed as median focus-forming units per mL (FFC/mL) of at least 3 independent experiments.