Figure 5
From: Small ruminant macrophage polarization may play a pivotal role on lentiviral infection
Virus-induced polarization of MDM. MDM were allowed to differentiate in the absence of cytokine and then infected with different SRLV strains (EV1, 496, CAEV-Cork, CAEV-To, Roccaverano and Seui) at a MOI of 0.1. Six day post-infection relative expression of the markers TNF-α (a), CD80 (b), A3Z1 (c), MR (d), DC-SIGN (e), and IL-10 (f) was measured by quantitative RT-PCR. The values are expressed as 2-ΔCt × 100, related to β-actin, and represent the median (± interquartile range) of at least 3 independent experiments.