Toosendanin activates caspase-1 and induces maturation of IL-1β to inhibit type 2 porcine reproductive and respiratory syndrome virus replication via an IFI16-dependent pathway
Veterinary Research volume 53, Article number: 61 (2022)
Porcine reproductive and respiratory syndrome virus (PRRSV) is a prevalent and endemic swine pathogen which causes significant economic losses in the global swine industry. Multiple vaccines have been developed to prevent PRRSV infection. However, they provide limited protection. Moreover, no effective therapeutic drugs are yet available. Therefore, there is an urgent need to develop novel antiviral strategies to prevent PRRSV infection and transmission. Here we report that Toosendanin (TSN), a tetracyclic triterpene found in the bark or fruits of Melia toosendan Sieb. et Zucc., strongly suppressed type 2 PRRSV replication in vitro in Marc-145 cells and ex vivo in primary porcine alveolar macrophages (PAMs) at sub-micromolar concentrations. The results of transcriptomics revealed that TSN up-regulated the expression of IFI16 in Marc-145 cells. Furthermore, we found that IFI16 silencing enhanced the replication of PRRSV in Marc-145 cells and that the anti-PRRSV activity of TSN was dampened by IFI16 silencing, suggesting that the inhibition of TSN against PRRSV replication is IFI16-dependent. In addition, we showed that TSN activated caspase-1 and induced maturation of IL-1β in an IFI16-dependent pathway. To verify the role of IL-1β in PRRSV infection, we analyzed the effect of exogenous rmIL-1β on PRRSV replication, and the results showed that exogenous IL-1β significantly inhibited PRRSV replication in Marc-145 cells and PAMs in a dose-dependent manner. Altogether, our findings indicate that TSN significantly inhibits PRRSV replication at very low concentrations (EC50: 0.16–0.20 μM) and may provide opportunities for developing novel anti-PRRSV agents.
Porcine reproductive and respiratory syndrome (PRRS) has been a great concern to pig industry globally since it was discovered in 1987 in the United States . It has been estimated that PRRS infection leads to at least $600 million losses annually to the US swine industry [2, 3]. PRRS virus (PRRSV), the etiological agent of PRRS, is an enveloped virus containing a single-stranded, positive-sense RNA genome of 15.4 kb in length. It belongs to the family of Arteriviridae in the order Nidovirales . Currently, the prevention of PRRS infection mainly depends on vaccination, including the use of inactivated and attenuated live vaccines. However, the vaccines usually provide limited protection against PRRS infection [5, 6] due to high mutation rate of PRRSV and that the circulation of multiple subtypes . Some compounds may act as potential anti-PRRSV agents, such as artesunate , glycyrrhizin , xanthohumol , platycodin D , (−)-epigallocatechin-3-gallate , and flavaspidic acid AB . Nevertheless, no effective drugs are commercially available for the treatment of PRRSV infection.
Innate immune defenses are essential for the control of virus infection . Inflammation is a tightly controlled process induced by the innate immune system in response to virus infection and is responsible for virus clearance and homeostasis restoration . It is triggered by the sensing of Pathogen-Associated Molecular Patterns (PAMPs) by Pattern Recognition Receptors (PRRs) [15, 16]. PRRs include the cytosolic receptors such as the membrane-anchored Toll-like receptors (TLRs), nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs), retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) and cyclic GMP-AMP synthase (cGAS) . It is well known that the gamma-interferon-inducible protein 16 (IFI16) acts as a nuclear sensor and a direct restriction factor of a variety of DNA viruses [18,19,20]. Existing evidence shows that IFI16 also directly senses viral RNA and enhances RIG-I transcription and activation to restrict RNA virus infection [21, 22]. Chang et al. showed that IFI16 plays a key role in regulating the type I IFN response to PRRSV, and overexpression of IFI16 inhibits PRRSV replication in Marc-145 cells . These findings indicate that upregulation of IFI16 can potentially combat PRRSV infection. However, it remains unclear whether IFI16 affects PRRSV infection in primary porcine alveolar macrophages (PAMs), which are a major target of PRRSV in pigs.
The proinflammatory cytokine IL-1β, mainly produced by macrophages, epithelial cells and neutrophils, plays important roles in host defense against virus infection [24, 25]. The caspase-1 activation itself is regulated by a multi-protein complex known as the inflammasome. The inflammasome is a caspase-1-activating molecular platform formed by interaction of three proteins, 1) a ‘‘sensor protein’’ recognizing the trigger, 2) an adaptor molecule known as the apoptosis associated speck-like protein containing CARD (ASC), and 3) procaspase-1. This platform provides the molecular scaffolds required for the proteolytic processing of inactive procaspase-1 in to active caspase-1 [26, 27]. IL-1β was found to have potent antiviral effects against the infection of a variety of viruses, including hepatitis B virus (HBV) , influenza A virus (IAV)  and African swine fever virus (ASFV) . However, the role of the IFI16 inflammasome and its downstream factor IL-1β in PRRSV infection remain unclear.
Toosendanin (TSN) is a tetracyclic triterpene extracted from the bark and fruits of Melia toosendan Sieb. et Zucc. It has been used as agricultural insecticide for decades and was ever used as digestive tract parasiticide in China [31,32,33]. In 2004, TSN was demonstrated to have a marked anti-botulismic effect in vivo and in vitro . After that, TSN was reported to possess anticancer activities by inducing apoptosis in various cancer cells [34,35,36]. In recent years, TSN was reported to have antiviral activities against IAV , hepatitis C virus (HCV) , severe fever with thrombocytopenia syndrome virus (SFTSV) and SARS-CoV-2 . However, the molecular mechanism of action for TSN against these viral infections remains poorly understood.
In the present study, we show that TSN potently inhibits PRRSV infection at sub-micromolar concentrations in a dose-dependent manner in Marc-145 cells and in PAMs. Furthermore, we demonstrate that inhibition of PRRSV infection by TSN is associated with the up-regulation of IFI16 and the activation of its downstream caspase-1/IL-1β signaling pathways. To our knowledge, this is the first report of the mechanisms responsible for TSN anti-PPRSV activities. Our work may provide valuable insight into the application of TSN as a novel antiviral against PRRS infection.
Materials and methods
Cell lines and viruses
Marc-145 cells, a PRRSV-permissive cell line derived from African green monkey kidney cell line MA-104 were obtained from the American Type Culture Collection (ATCC) and grown in Dulbecco’s minimum essential media (DMEM, Gibco, UT, USA) supplemented with 10% fetal bovine serum (FBS, Biological Industries, Kibbutz Beit Haemek, Israel) and 100 IU/mL of penicillin and 100 g/mL streptomycin at 37 °C with 5% CO2.
Porcine alveolar macrophages (PAMs) were obtained from the lungs of 4- to 6-week-old PRRSV-negative Large-White piglets (Xinli Pig Farm, Wuzhou, China) by lung lavage according to a previously described method . Briefly, the lungs were washed three times with pre-cooled phosphate buffered saline (PBS) solution containing penicillin (300 IU/mL) and streptomycin (300 g/mL). Cells were centrifuged at 800 g for 10 min, resuspended in RPMI 1640 supplemented with 10% FBS and 100 IU/mL of penicillin and 100 g/mL streptomycin at 1 × 106 cells/mL in 6-well plate, and then incubated at 37 °C for 2 h.
Three type 2 PRRSV strains including classical CH-1a and VR-2332 and the highly pathogenic PRRSV NADC30-like HNhx strain (GenBank no. KX766379)  were propagated in Marc-145 cells in DMEM containing 3% FBS (essential media). Virus preparations were titrated and stored at −80 ℃. Virus titer was determined using a microtitration infectivity assay .
Preparation of toosendanin and chemicals
Toosendanin (TSN) and Platycodin D (PD) were purchased from Chengdu Pu fei De Biotech Co., Ltd. (Chengdu, China), with a purity of 99.3%. Ribavirin (Rib), a broad-spectrum antiviral was purchased from Star Lake Bioscience Co., Ltd. (Zhaoqing, China). It was dissolved in dimethyl sulfoxide (DMSO, Sigma-Aldrich, MA, USA) and diluted with essential medium before use. The final concentration of DMSO in the culture media was less than 0.4%. Recombinant porcine Interleukin-1β (rmIL-1β) was purchased from Beyotime Biotechnology (Shanghai, China). It was reconstituted in sterile distilled water to make up a 0.1–1.0 mg/mL stock solutions. The rmIL-1β stock solutions were aliquoted and stored at −20 ℃.
The cytotoxicity of TSN was evaluated using MTT assay . Briefly, Marc-145 cells (5 × 104 cells/well) and PAMs (2 × 105 cells/well) were seeded in 96-well plates and grown at 37 °C for 24 h and 6 h, respectively. The media were then replaced with fresh media containing serial dilutions of TSN, followed by incubation for 48 h. After removal of the culture media, the cells were incubated with 100 μL 3-(4,5-dimethylthiozol-2-yl)-3, 5-dipheryl tetrazolium bromide (MTT; Sigma-Aldrich) solution (0.5 mg/mL in PBS) at 37 °C for 4 h. The MTT was then removed, and 150 μL of DMSO was added into each well to dissolve the formazan crystals for 10 min at 37 °C. Cell viability was measured by absorbance at 490 nm with a microplate reader (Thermo fisher scientific, MA, USA) and expressed as a percentage of the control level. The mean optical density (OD) values from six wells per treatment were used as the cell viability index. The 50% cytotoxic concentration (CC50) was analyzed by GraphPad Prism 5.0 (GraphPad Software, San Diego, CA, USA).
Viral titer titration
Viral titer was established with the endpoint dilution assay described as before . Briefly, viral preparations were serially diluted in essential media in tenfold increments, and 100 µL of each diluted preparation was inoculated in quadruplicate to confluent monolayers of Marc-145 cells in 96-well plates, followed by incubation for 2 h at 37 °C. The inoculum was then aspirated out from each well, and the cell monolayers were replenished with fresh essential media and cultured for an additional 72 h to monitor daily the cytopathic effects (CPE). Virus titer was calculated based on the amount of CPE and expressed as a 50% tissue culture infective dose (TCID50)/mL.
Indirect immunofluorescence assay (IFA)
For immunostaining, the PRRSV-infected or uninfected cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.25% Triton X-100 for 10 min at room temperature (RT), blocked with 1% bovine serum albumin (BSA) for 60 min at RT and then incubated with a mouse monoclonal antibody against the N-protein of PRRSV (clone 4A5, 1:400 dilution, MEDIAN Diagnostics, Korea) at 4 ℃ overnight. After three washes with PBS, the cells were incubated for 1 h at RT with a goat anti-mouse secondary antibody conjugated with Alexa Fluor 568 (red) (Cell Signaling Technology, MA, USA) at 1:1000 dilution. Nuclei were counterstained using 50 μL of 4,6-diamidino-2-phenylindole (DAPI, 300 nM; Sigma-Aldrich) (blue). Immunofluorescence was captured using the Leica DMI 4000B fluorescence microscope (Leica, Wetzlar, Germany). Blue and red fluorescence spots were counted as the total and PRRSV-infected cell number, respectively, in every IFA image. The percentage of infected cell counts among total cell counts was considered as the infection rate. Relative infected-cell percentage was determined by the ratio of the infection rate in TSN-treated groups to that in DMSO-treated control. The EC50 value (the concentration required to protect 50% cells from PRRSV infection) was determined by plotting the relative infected-cell percentage as a function of compound concentration and calculated with the GraphPad Prism 5.0 software.
Real-Time quantitative reverse-transcription PCR (qRT-PCR)
Total RNA was extracted using the total RNA rapid extraction kit (Fastagen, Shanghai, China) following the manufacturer’s instructions. RNA was reverse-transcribed into first-strand cDNA using a reverse transcription kit (Genstar, Beijing, China). PCR amplification was performed on 1 μL of reverse-transcribed product with primers designed against PRRSV-NSP9, IFI16 and GAPDH (glyceraldehyde-3-phosphate dehydrogenase, used as endogenous control). The primers used for PCR amplification are listed in Table 1 [13, 23]. Real-time PCR was performed using 2 RealStar Green Power Mixture (containing SYBR Green I Dye) (Genstar, Beijing, China) on the CFX96 Real-time PCR system (Bio-Rad, CA, USA). Relative mRNA expression was calculated by 2−ΔΔCT method using DMSO-treated infected cells or DMSO-treated mock-infected cells as reference samples for determining PRRSV-NSP9 and IFI16 gene expression, respectively [43, 44].
Western blot analysis
PRRSV-infected or uninfected Marc-145 cells treated with TSN were lysed in RIPA lysis buffer containing 1 mM phenylmethylsulfonylfluoride (Beyotime, Shanghai, China) at 4 ℃. The supernatant was harvested after centrifugation (15 000 rpm for 30 min at 4 ℃) and the total protein for each sample measured using the BCA protein assay kit (Beyotime, China). 10 μg of total protein per sample was electrophoresed onto 12% SDS-PAGE gels and transferred onto polyvinylidene-fluoride (PVDF) membranes (Millipore, MA, USA). After blocking, membranes were incubated with a mouse anti-PRRSV N-protein monoclonal antibody (clone 4A5, MEDIAN Diagnostics, Chuncheon, Korea), IFI16 rabbit polyclonal Antibody (Beyotime, Shanghai, China), Gasdermin D rabbit monoclonal antibody (Cell Signaling Technology, MA, USA), Cleaved Gasdermin D rabbit monoclonal antibody (Cell Signaling Technology, MA, USA), pro-caspase-1 rabbit monoclonal antibody (Cell Signaling Technology, MA, USA), caspase-1 rabbit monoclonal antibody (Cell Signaling Technology, MA, USA), IL-1β rabbit monoclonal antibody (Cell Signaling Technology, MA, USA) or a mouse anti-GAPDH monoclonal antibody (GoodHere, Hangzhou, China) at 1:1000 dilution at 4 ℃ overnight. HRP conjugated goat anti-mouse or anti-rabbit IgG (H–L) (1:3000; Beyotime, Shanghai, China) was used as the secondary antibody for 1 h incubation at RT. The Western blot results were visualized using the chemiluminescence technology.
Antiviral activity assay
The in vitro antiviral activity assay was performed to examine the capacity of TSN to inhibit PRRSV replication. Marc-145 cells or PAMs in 96-well plates were infected with PRRSV at a multiplicity of infection (MOI) of 0.05 for Marc-145 cells and 0.5 for PAMs in essential medium for 2 h at 37 ℃. The culture supernatants were then removed and fresh DMEM containing different concentrations of each compound were added into each well. Cells and supernatants were then collected at the indicated time points post infection, and their virus titer was determined by the endpoint dilution assay and viral RNA level by qRT-PCR. Cells were subjected to PRRSV-infected cell counting by IFA, and their viral NP level was determined by Western blotting.
Direct PRRSV-TSN interaction
To investigate whether TSN directly interact with the virus, 100 μL of PRRSV (0.5 MOI) was mixed with various TSN concentrations in essential medium (0.9 mL) and incubated for 1 h at 37 ℃. Then, PRRSV and TSN were separated by ultrafiltration centrifugation. PRRSV trapped in the ultrafiltration tube were washed three times with essential medium to remove residual TSN, and were then resuspended in essential medium to infect Marc-145 cells grown in 12-well plates for 2 h. After three washes with PBS, the cells were cultured in fresh medium for an additional 48 h at 37 ℃ and then subjected to viral mRNA and N protein using qRT-PCR and IFA.
TSN pretreatment on Marc-145 cells
To investigate whether TSN inhibits PRRSV replication through altering the susceptibility of host cells, Marc-145 cells were pretreated with TSN in essential medium for 2 h at 37 ℃. After three washes with PBS, cells were infected with PRRSV (0.05 MOI) for 2 h and collected at 48 h post-infection (hpi) for qRT-PCR and IFA.
RNA collection, preparation, transcriptome sequencing, and functional analysis
Marc-145 were grown in 6-well plates were infected with PRRSV (0.05 MOI) in essential medium for 2 h at 37 ℃. Supernatants were removed and fresh DMEM containing 1.6 μM of TSN or DMSO were added and incubated for 2 h and then the cells were collected for transcriptomic analysis. Transcriptome sequencing was performed in the Novogene Bioinformatics Institute (Novogene, Beijing, China). Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer (5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order to select cDNA fragments of preferentially 370–420 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer instructions. After cluster generation, the library preparations were sequenced on an Illumina Novaseq platform and 150 bp paired-end reads were generated. Feature Counts v1.5.0-p3 was used to count the reads numbers mapped to each gene. The fragments per kilo base per million (FPKM) of each gene was calculated based on the length of the gene and reads count mapped to this gene. Different expression analysis of two conditions/groups (two biological replicates per condition) was performed using the DESeq2 R package (1.20.0). DESeq2 provides statistical routines for determining differential expression in digital gene expression data using a model based on the negative binomial distribution. The resulting P-values were adjusted and the Benja mini and Hochberg approach was used to decrease the false discovery rate. Genes with an adjusted P-value < 0.05 found by DESeq2 were assigned as differentially expressed. We used cluster Profiler R package to test the statistical enrichment of differential expression genes in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways.
Gene silencing with siRNA
The specific siRNAs targeting IFI16 (si-IFI16) and the control siRNA (siNC) were synthesized by Sangon Biotech. The siRNA sequences are as follows: siIFI16, 5′-CCAAGCAGCAGUUGCUUAATT-3′; the nontargeting control siRNA (siNC), 5′-UUCUCCGAACGUGUCACGUTT-3′ . Marc-145 cells were transfected with 50 nM siRNA using 5 µL of Lipofectamine™ 3000 Transfection Reagent (Thermo Scientific, CA, USA). 24 h post-transfection (hpt), the transfected cells were infected with PRRSV and treated with TSN (0.4 μM). Supernatants and lysates of the cells were then collected at 24 hpi for further analysis.
Determination of GSDMD-N, Caspase-1 and IL-1β levels
Marc-145 cells or PAMs in 6-well plates were infected with PRRSV (0.05 MOI for Marc-145 cells and 0.5 MOI for PAMs) in essential medium for 2 h at 37 ℃. The culture supernatants were then removed and fresh DMEM containing different TSN concentrations were added into each well. Cells were cultured in fresh medium for an additional 24 h at 37 ℃ and then subjected to GSDMD-N, Caspase-1 and IL-1β protein using Western blotting.
All experiments were performed at least three times. The results were presented as mean standard deviation (SD). Statistical significance was determined by the Student’s t test when only two groups were compared or by one-way analysis of variance (ANOVA) when more than two groups were compared. *p < 0.05, **p < 0.01, and ***p < 0.001 were considered to be statistically significant at different levels.
TSN inhibits PRRSV infection in Marc-145 cells
The chemical structure of TSN is shown in Figure 1A. We first tested the cytotoxic effect of TSN on Marc-145 cells using MTT assay. As shown in Figure 1B, TSN did not affect Marc-145 cell viability when the concentration was increased to 16 μM. The 50% cytotoxic concentration (CC50) of TSN on Marc-145 cells was 42.32 μM. Next, we examined the antiviral effects of TSN against three different PRRSV strains (NADC30-like, VR-2333 and CH-1a). Marc-145 cells were infected by PRRSV for 48 h, fixed and then imaged by immunofluorescence microscopy. As shown in Figure 1C, D, NADC30-like, VR-2333 and CH-1a infections in Marc-145 cells were significantly inhibited by TSN in a dose-dependent manner. The half maximal effective concentrations (EC50) and the corresponding selectivity indexes (SI) were calculated based on the infected cell percentages to determine the antiviral activities of TSN on the three PRRSV strains. As shown in Table 2, the EC50 of TSN against the infections of NADC30-like, VR-2333 and CH-1a was 0.20, 0.18 and 0.16 μM, and the corresponding SI was 211, 235 and 264, respectively. These results indicate that TSN inhibited PRRSV infections in Marc-145 cells at concentrations far lower than its maximum non-cytotoxic concentration. Ribavirin, a well-known inhibitor of viral RNA polymerase, was reported to have anti-PRRSV activity  and thus used as the positive antiviral drug control in this study. Our results showed that 160 µM of ribavirin exhibited significant inhibition on the three PRRSV strains (Figure 1C, D). Strikingly, the inhibitory effects of TSN were overall stronger than those of ribavirin.
We further examined the effects of TSN on PRRSV replication by infecting Marc-145 cells with different MOI (0.03, 0.3 and 3) of NADC30-like strain using virus titration, qRT-PCR, and Western blotting. As shown in Figure 2A, treatment with TSN resulted in a significant reduction of PRRSV titer in a dose-dependent manner at three different NADC30-like infection doses. Treatment with 1.6 µM of TSN led to 5, 7, 9 log reduction in progeny virus production compared to that in DMSO-treated control (Figure 2A). TSN at concentrations ranging from 0.1 to 1.6 µM significantly inhibited NADC30-like NSP9 mRNA and N protein levels in infected Marc-145 cells (Figure 2B, C). We next studied the PRRSV inhibition kinetics by three different concentrations of TSN from 24 to 72 hpi. In the infected control (PRRSV), the virus titer, viral N protein and viral NSP9 mRNA expressions increased markedly from 24 to 48 hpi, and viral NSP9 mRNA expressions decreased from 48 to 72 hpi (Figure 2D–F). The addition of TSN (0.1, 0.4 and 1.6 µM) significantly reduced virus titer, viral N protein and NSP9 mRNA levels at all time-points. The effects were more potent than 160 µM of Rib as shown in Figure 2D–F. Collectively, these data demonstrate that TSN significantly inhibits PRRSV NADC30-like replication in Marc-145 cells.
TSN inhibits PRRSV infection in porcine alveolar macrophages
Since TSN exerted a potent antiviral activity against PRRSV infection in Marc-145 cells, we therefore investigated whether TSN could also inhibit PRRSV ex vivo replication in PAMs, the major target cell type of PRRSV infection in pigs. We first assessed TSN cytotoxicity on PAMs using MTT assay. As shown in Figure 3A, TSN exhibited a cytotoxicity profile on PAMs like that on Marc-145 cells, with 16 µM of TSN found non-cytotoxic to PAMs and the CC50 determined as 31.49 µM. Next, we evaluated the antiviral effects of TSN against PRRSV NADC30-like replication in PAMs using virus titration and IFA. As shown in Figure 3B–D, TSN significantly reduced progeny virus production and N protein level; treatment with 1.6 µM of TSN resulted in a 3.8 log reduction in progeny virus production when compared to that in the DMSO control (Figure 3B). Inhibition of TSN on NADC30-like replication in PAMs from 24 to 48 hpi was also observed by qRT-PCR and Western blotting. As shown in Figure 3E, F, the addition of 1.6 µM of TSN significantly reduced viral NSP9 mRNA and N protein levels at all time-points. Again, the effects were more potent than 160 µM of Rib. Taken together, TSN could effectively inhibit PRRSV NADC30-like infection in ex vivo PAMs.
TSN does not interact directly with PRRSV
As demonstrated above, TSN exerted a remarkable antiviral activity against PRRSV infection in Marc-145 cells and PAMs. We thus questioned whether TSN could inactivate PRRSV by directly interacting with the virus, thereby inhibiting virus infection. To address this question, TSN (at final concentrations of 0.1, 0.4 and 1.6 μM) was mixed with PRRSV NADC30-like, incubated for 1 h at 37 ℃. Then PRRSV and TSN were separated by ultrafiltration, as shown in Figure 4A. Recovered PRRSV were resuspended to infect Marc-145 cells. At 48 hpi, the cells were subjected to viral mRNA expression and PRRSV-infected cell ratio analysis using qRT-PCR and IFA, respectively. As shown in Figure 4B–D, co-incubation of TSN (0.1, 0.4, and 1.6 µM) with the virus did not weaken PRRSV ability to infect Marc-145 cells, demonstrating that TSN does not directly interact with PRRSV particles. Platycodin D (PD), a triterpenoid saponin, which was reported to inhibit PRRSV replication by directly interacting with the virions, was used as positive control in this study .
TSN pretreatment significantly reduces PRRSV replication
Having excluded TSN-virus direct interaction, we wanted to identify whether TSN could potentially alter the cell susceptibility to PRRSV. Marc-145 cells were treated with 0.1, 0.4 or 1.6 μM of TSN before infection (Figure 5A). As shown in Figure 5B–D, the level of viral mRNA and N protein were significantly reduced by TSN pretreatment; pretreatment with 1.6 µM of TSN resulted in a 100% protection from PRRSV infection. These results indicate that TSN interacts with cellular components to reduce PRRSV replication.
TSN treatment upregulates IFI16 expression in Marc-145 cells
To investigate the mechanism underlying TSN anti-PRRSV effect, we performed high-throughput RNA sequencing (RNA-seq) to explore gene expression in Marc-145 cells that had been treated with or without TSN for 2 h. KEGG analysis showed that differentially expressed genes (DEGs) were significantly enriched in the NOD-like receptor signaling pathway, as marked by asterisks (Figure 6A). We therefore investigated a large set of genes associated with the NOD-like receptor signaling pathway, including NOD1, NOD2, NLRC4, NLRC12, NLRP7, NLRP3 and IFI16. Notably, our results unveiled that TSN upregulated the expression of IFI16 in both uninfected and infected Marc-145 cells (Figure 6B), indicating that the protective effects of TNS against PRRSV infection might be associated with enhanced IFI16 expression. To confirm the results of the RNA-seq analysis, the effects of TSN on IFI16 mRNA expression were measured by qRT-PCR at 24 hpi. As shown in Figure 6C, TSN significantly upregulated IFI16 mRNA expression in both uninfected and infected Marc-145 cells. To further explore the role of TSN-induced IFI16 in PRRSV-infected cells, uninfected (Figure 6D) and infected (Figure 6E) Marc-145 cells were treated with TSN at various concentrations for 12 h, 24 h or 36 h, followed by Western blot analysis for IFI16 protein expression. The results showed that TSN gradually upregulated IFI16 protein expression in both uninfected and infected cells as the treatment time increased. Previous studies highlight IFI16 as a critical antiviral factor during virus infection [18, 46], we speculated that the inhibition of TSN on PRRSV replication was related to the upregulation of IFI16 protein expression.
TSN inhibits PRRSV replication via up-regulating IFI16 expression
Since TSN upregulated IFI16 expression in both PRRSV-infected and -uninfected Marc-145 cells, we questioned whether this upregulation may contribute to TSN anti-PRRSV activity. We therefore designed specific siRNAs to inhibit IFI16 expression (Figure 7A, B). Silencing IFI16 resulted in enhanced PRRSV replication as shown by the increased levels of viral NSP9 mRNA in Figure 7C. Similarly, viral N protein was also markedly increased following IFI16 silencing (Figure 7D–F). Importantly, we found that IFI16 silencing impacted TSN anti-PRRSV activity reflected by increased PRRSV replication (Figure 7C), a 56% increase in viral N protein expression (Figure 7E, F). These results indicate that TSN inhibits PRRSV replication via up-regulating IFI16 expression.
TSN enhances caspase-1-mediated maturation of IL-1β to inhibit PRRSV replication via an IFI16-dependent pathway in Marc-145 cells
DNA recognition of pathogenic microorganisms by IFI16 leads to the activation of the caspase-1-dependent inflammasome and the maturation of IL-1β . To identify the role of caspase-1-dependent inflammasome in the inhibition of PRRSV infection by TSN, Marc-145 cells were infected with PRRSV and the PRRSV N protein and a set of proteins in the casepase-1 signaling pathway, including caspase-1, pro-caspase-1, GSDMD-N, GSDMD-F and IL-1β were analyzed by Western blotting. As shown in Figure 8A, there was no significant change in the expressions of those casepase-1 regulated proteins following PRRSV infection, while TSN treatment significantly upregulated the expression of caspase-1 and its downstream target proteins (GSDMD-N, IL-1β). Meanwhile, a decrease in virus replication, reflected by attenuated N-protein expression, was also observed in TNS-treated infected cells. To further identify the effect of IFI16-driven activation on the caspase-1 signaling pathway, Marc-145 cells were transfected with IFI16 siRNA or negative siRNA (siNC) for 24 h followed by PRRSV infection and then TSN treatment (0.4 μM). As shown in Figure 8B, IFI16 silencing decreased the overexpression of caspase-1, GSDMD-1 and IL-1β induced by TSN treatment in PRRSV-infected Marc-145 cells.
Since IL-1β plays an important role in antiviral host defense , we therefore investigated the antiviral effect of IL-1β against PRRSV infection. For this purpose, Marc-145 cells were infected with PRRSV NADC30-like (0.05 MOI) for 2 h at 37 ℃ and then cultured in fresh media containing various concentrations of IL-1β for 24 h followed by qRT-PCR, Western blotting and IFA. As expected, exogenous IL-1β significantly reduced viral NSP9 mRNA and N protein levels in Marc-145 cells (Figure 8C, D), treatment with 0.5 µg/mL of IL-1β resulted in an 88% reduction of viral N protein when compared to that in the DMSO control (Figure 8E, F). These data together demonstrate that TSN enhances caspase-1-mediated production of IL-1β to inhibit PRRSV replication through an IFI16-dependent pathway.
TSN inhibits PRRSV replication in PAMs via promoting the IFI16/caspase-1/IL-1β pathway
Given that PAMs are natural target cells in pigs, we investigated whether TSN enhances caspase-1-mediated IL-1β production by up-regulating IFI16, thereby inhibiting PRRSV replication in PAMs. PRRSV-infected and uninfected PAMs were treated with 1.6 μM of TSN. At the indicated time points, the expression levels of IFI16 and N protein were analyzed by Western blotting. As shown in Figure 9A–D, TSN continuously induced the expression of IFI16 and inhibited PRRSV replication (reflected by reduced N protein expression) in PAMs. TSN also induced overexpression of caspase-1, GSDMD-N, and IL-1β in uninfected and in PRRSV-infected PAMs in a dose-dependent manner (Figure 9E), as in Marc-145 cells. Finally, we explored whether IL-1β could also inhibit PRRSV replication in PAMs. As shown in Figure 9F-I, IL-1β treatment significantly inhibited PRRSV replication in a dose-dependent manner in PAMs, as indicated by the reduced levels of viral NSP9 mRNA and N protein. At 24 hpi, treatment with 0.5 µg/mL of IL-1β resulted in an 92% reduction of viral N protein when compared to that in the DMSO control (Figure 9G, I). These data demonstrate that TSN promotes caspase-1-mediated maturation of IL-1β to inhibit PRRSV replication in PAMs also via the IFI16-dependent pathway.
Toosendanin (TSN), a natural triterpenoid compound, was initially extracted from the bark of Melia toosendan Seib. et Zucc. (a traditional Chinese medicine) in the 1950’s and was then used as an ascarifuge in China . Previous research indicated that TSN possesses broad biological activities, such as blocking acetylcholine release, and anti-botulismic, and anti-tumor effects [47, 48]. Importantly, TSN was reported to inhibit infections of viruses such as HCV , IAV , SFTSV and SARS-CoV-2 . However, these studies mainly focused on TSN antiviral activities rather than its mechanisms of action. The present study is the first to show that TSN potently inhibits PRRSV infection at sub-micromolar concentrations in Marc-145 cells and in ex vivo PAMs. Mechanistically, we demonstrate that TSN induces IFI16 expression, which leads to increased expression of caspase-1 and IL-1β, the later contributing to the inhibition of PRRSV replication in the infected cells.
As TSN exhibited a potent inhibitory activity against PRRSV at very low concentrations in Marc-145 cells and PAMs (Figures 1, 2, 3), we questioned whether TSN could inactivate PRRSV by directly interacting with virus particles, thereby inhibiting the infection. To address this question, we designed a series of experiments (Figures 4 and 5). Surprisingly, pretreatment of TSN with cells, rather than with viruses, significantly reduced PRRSV replication (Figure 5), suggesting that TSN may act on the cells to inhibit PRRSV infection but not directly inactivate PRRSV. This prompts us to perform a transcriptome analysis on PRRSV-infected and uninfected Marc-145 cells that were treated with or without TSN. Of the five hundred thirty-three differentially regulated genes, IFI16 was one of the most significantly upregulated genes in TSN-treated PRRSV-infected Marc-145 cells. The upregulation of IFI16 was also observed in TSN-treated uninfected Marc-145 cells (Figures 6A, B). IFI16 is a member of the pyrin and HIN domain (PYHIN) containing protein family, which includes key mediators of the innate immune response that sense microbial DNAs to induce IFNs and/or inflammasome activation [18, 49]. Previous studies have shown that IFI16 inhibits human cytomegalovirus (HCMV) transcription  and restricts herpes simplex virus 1 (HSV-1) replication . In recent years, the role of IFI16 in RNA virus infections has been identified, whereby IFI16 transcriptionally upregulates the gene expression of IFN-I in Sendai virus (SeV) and mouse hepatitis coronavirus infection [22, 50, 51]. More recently, Wichit et al. found that enhanced expression of IFI16 completely restricted Chikungunya virus (CHIKV) infection while endogenous silencing of the gene markedly increased virus replication . These findings suggest that IFI16 is a key positive regulator in antiviral innate immune responses. However, inducers for IFI16 were barely reported. The present study shows that TSN inhibit PRRSV replication via enhancing IFI16 expression in Marc-145 cells (Figure 6C–E) and PAMs (Figure 9A–D). More importantly, these effects were reversed by silencing the IFI16 gene (Figures 7 and 8), corroborating that the anti-PRRSV activity of TSN is IFI16-dependent. Thus, our findings demonstrate that TSN is a potent inducer of the IFI16 and might serve as a therapeutic agent against PRRSV infection.
IFI16 inflammasome is a large signaling platform activated by IFI16 in response to viral infection, which also contains the adapter protein ASC (apoptosis-associated speck-like protein containing a caspase recruitment domain) and effector enzyme pro-caspase-1 [27, 53]. Inflammasome activation was examined by degradation of pro-caspase-1 to caspase-1 . IL-1β is generated via proteolytic cleavage of pro-IL-1β by caspase-1 during inflammasome activation [55, 56]. Here, we demonstrated that TSN displayed anti-PRRSV activity by activating IFI16 inflammasome. TSN treatment enhanced expressions of caspase-1, GSDMD-N and IL-1β in a dose-dependent manner in PRRSV-infected Marc-145 cells and PAMs (Figures 8A and 9E), and these effects were reversed by silencing the IFI16 gene, indicating that TSN enhances caspase-1-mediated maturation of IL-1β to inhibit PRRSV replication in an IFI16-dependent pathway. IL-1β is an important component of the host defense against viral infection, due to its key role in innate immunity . There is ample evidence showing that the activation of inflammasomes in the airway epithelium and concomitant mucosal production of IL-1β and IL-18 are crucial for optimal antibody and T cell responses against IAV [29, 58, 59]. In addition, Li et al. found that downregulation of pMGF505-7R expression induces higher levels of IL-1β production which lowers pathogenicity in pigs with ASFV infection, and enhance viral clearance and infected-host survival [30, 60]. Song et al. found that 25-Hydroxycholesterol significantly decreased the replication of PRRSV, and increased the production of IL-1β and IL-8 in porcine primary alveolar macrophages and the lung tissue . In this work, we extend IL-1β inhibiting role on PRRSV replication (Figures 8C–F and 9F-I). It can be concluded that TSN inhibits PRRSV infection partly by up-regulating the expression of IL-1β. Recently, Lauren et al. showed that IL-1β induced antiviral interferon responses against West Nile virus infection in dendritic cells through a process of IL-1β-to-IRF3 signaling crosstalk . In addition, Lanaya et al. showed that IL-1β stimulation of Kupffer cells enhanced ADAM17 expression at both transcriptional and proteic levels . ADAM17 is one of the important membrane-associated metalloproteases that mediates various cellular events as well as inflammation, cancer, and other pathologies . Guo et al. confirmed that ADAM17 is able to suppress PRRSV entry by down-regulating the expression of membrane CD163, which is an essential viral receptor mediating PRRSV entry and uncoating . A schematic summary for the inhibitory cascade of TSN on PRRSV replication is proposed in Figure 10. However, the exact mechanism by which elevated IL-1β suppresses PRRSV replication needs to be further investigated.
In our study, TSN exhibited potent inhibition against PRRSV replication at concentrations ranging from 0.1 to 1.6 μM in vitro, raising the question of whether such concentrations could be attainable in pigs when TSN is administrated at safe doses. Wang et al. reported that the average peak plasma drug concentrations of TSN in rat (single 2 mg/kg of body weight intravenous injection) range from 1.5 to 7.76 μM , which are much higher than the effective anti-PRRSV concentrations in our in vitro experiments. It is therefore reasonable to speculate that therapeutical TSN concentrations are reachable in PRRSV-infected pigs.
In summary, our study shows that TSN potently suppresses PRRSV replication at sub-micromolar concentrations. Furthermore, we elucidate the mechanisms underlying antiviral effect of TSN against PRRSV infections as activating caspase-1 to induce IL-1β maturation via IFI16-inflammasome. TSN therefore has the potential to become a novel antiviral agent.
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The authors wish to thank Ph.D student Wei Wang (College of Veterinary Medicine, South China Agricultural University) for sharing her extensive knowledge of gene silencing. In addition, the critical and helpful comments from the reviewers are highly appreciated.
This work was funded by the National Natural Science Foundation of China (Grant Numbers 31872521 and 31941019), Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (Grant 2019BT02N054), and the Basic Research & Applying Basic Research Foundation of Guangdong Province (Grant Number 2019B1515210007).
The authors declare that they have no competing interests.
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Zhang, M., Lu, C., Su, L. et al. Toosendanin activates caspase-1 and induces maturation of IL-1β to inhibit type 2 porcine reproductive and respiratory syndrome virus replication via an IFI16-dependent pathway. Vet Res 53, 61 (2022). https://doi.org/10.1186/s13567-022-01077-2
- Porcine reproductive and respiratory syndrome virus (PRRSV)
- Toosendanin (TSN)
- inhibitory activity
- gamma-interferon-inducible protein 16 (IFI16)
- caspase-1; Interleukin-1β (IL-1β)