Figure 8

TSN enhances the production of IL-1β to inhibit the replication of PRRSV. (A) Marc-145 cells grown in 6-well plates were infected with PRRSV NADC30-like (0.05 MOI) for 2 h at 37 ℃ and then cultured in fresh media containing various concentrations of TSN. (B) Marc-145 cells were transfected with siRNA targeting IFI16. At 24 hpt, the transfected cells were infected with PRRSV at 0.05 MOI and then cultured in fresh media containing TSN (0.4 μM). After 24 h, the cells were harvested for Western blot analyses to detect the level of IFI16, caspase-1, pro-caspase-1, GSDMD-N, GSDMD-F, IL-1β, viral N protein and GAPDH. (C–F) Marc-145 cells grown in 24-well plates were infected with PRRSV NADC30-like (0.05 MOI) for 2 h at 37 ℃ and then cultured in fresh media containing various concentrations of IL-1β. At 24 hpi, the samples were subjected to qRT-PCR (C), Western blotting (D), or IFA (F). Scale bar: 100 μm. Statistical significances are denoted by *p < 0.05, **p < 0.01, and ***p < 0.001.