Figure 7

TSN upregulates IFI16 to inhibit PRRSV replication. (A and B) Marc-145 cells were transfected with siRNA targeting IFI16 and siNC. Total RNA and protein of the samples were extracted and subjected to IFI16 mRNA (A) and protein (B) analysis using qRT-PCR and Western blotting, respectively. (C–F) Marc-145 cells were transfected with siRNA targeting IFI16 or siNC. At 24 h post-transfection (hpt), the transfected cells were infected with PRRSV (0.05 MOI) for 2 h at 37 ℃ and then cultured in fresh medium containing 0.4 μM TSN. At 24 hpi, the samples were subjected to qRT-PCR (C), Western blotting (D), or IFA (E and F) analysis. Scale bar: 100 μm. Statistical significances are denoted by *p < 0.05, **p < 0.01, and ***p < 0.001.