- Research article
- Open Access
Stability of BSE infectivity towards heat treatment even after proteolytic removal of prion protein
Veterinary Research volume 52, Article number: 59 (2021)
The unconventional infectious agents of transmissible spongiform encephalopathies (TSEs) are prions. Their infectivity co-appears with PrPSc, aberrant depositions of the host’s cellular prion protein (PrPC). Successive heat treatment in the presence of detergent and proteolysis by a keratinase from Bacillus licheniformis PWD-1 was shown before to destroy PrPSc from bovine TSE (BSE) and sheep scrapie diseased brain, however data regarding expected reduction of infectivity were still lacking. Therefore, transgenic Tgbov XV mice which are highly BSE susceptible were used to quantify infectivity before and after the bovine brain treatment procedure. Also four immunochemical analyses were applied to compare the levels of PrPSc. After heating at 115 °C with or without subsequent proteolysis, the original BSE infectivity of 106.2–6.4 ID50 g−1 was reduced to a remaining infectivity of 104.6–5.7 ID50 g−1 while strain characteristics were unaltered, even after precipitation with methanol. Surprisingly, PrPSc depletion was 5–800 times higher than the loss of infectivity. Similar treatment was applied on other prion strains, which were CWD1 in bank voles, 263 K scrapie in hamsters and sheep PG127 scrapie in tg338 ovinized mice. In these strains however, infectivity was already destroyed by heat only. These findings show the unusual heat resistance of BSE and support a role for an additional factor in prion formation as suggested elsewhere when producing prions from PrPC. Leftover material in the remaining PrPSc depleted BSE preparation offers a unique substrate for searching additional elements for prion infectivity and improving our concept about the nature of prions.
Prions are infectious agents of transmissible spongiform encephalopathies (TSEs) or prion diseases . The infectivity is dependent on a conformationally malformed state (PrPSc) of the physiological protein PrPC, a cellular membrane protein with an as yet unclear function. The mechanism of transformation of this host encoded PrPC to PrPSc includes refolding and aggregation. PrPSc is partially resistant to digestion with proteolytic enzymes, usually proteinase K (PK). During proteolysis—often in the presence of detergent—the PrPSc molecules become N-terminally truncated while the remaining C-terminal part (PrPres) after dissociation and unfolding is characterized by a triplet of a diglycosylated, monoglycosylated and non-glycosylated PrP fragment in the 18–30 kDa molecular mass range.
The proof that PrPSc represents infectivity was first based on biomathematical and extensive biochemical work with hamster scrapie [1,2,3,4,5]. Definitive proof that the presence of PrPC is a prerequisite for TSE infection was presented from PrP-less mice, goats and cells, and by the production of infectivity from recombinantly expressed and purified PrP [6,7,8,9,10,11,12,13,14]. Another argument for the validity of the role of PrP in the agent is the close relation between susceptibility/resistance in e.g. sheep, goats and humans and genetic polymorphisms in the PRNP coding region [15,16,17]. Yet, the very reproducible strain properties characteristic for TSEs are not yet explained sofar. These might be dependent on the presence of lipid, polyanionic glycans or nucleic acid fragments in the agent or during PrPSc formation [9, 13, 18,19,20]. From observations with bovine spongiform encephalopathy (BSE) inoculated in in-bred wildtype mice it was even postulated that an additional unidentified agent may be essential for transmission while PrPSc would be involved in species adaptation .Footnote 1
Previously, we found that B. licheniformis PWD1 keratinase (KE) at 50 °C could reduce PrPSc by more than 99.9% after autoclaving for 40 min at 115 °C in the presence of the detergent sarkosyl at neutral pH . The material used in these experiments was brain stem from cattle and sheep clinically affected respectively by BSE and scrapie. Other investigators found proteases which already had a substantial PrPSc degrading effect even without heating above 100 °C or the presence of detergents in the homogenate while pH varied between 7–12 and temperatures between 37–70 °C [23,24,25]. According to these results, there is no direct correlation between PrPSc level and infectivity. This weakens the prion hypothesis which in part is based on a positive correlation between the two parameters [5, 26]. Further confusing are examples of infectivity related to protease sensitive PrPSc [27,28,29].
In this study we investigated whether our PrPSc removal from BSE infected cow brain using heating at 115 °C and enzymatic proteolysis goes together with removal of infectivity in the highly sensitive transgenic Tgbov XV mice expressing bovine PrP. The presence of PrPSc was tested in Western blotting and several biochemical methods. We also compared the effect of this brain treating methodology when applied on three other prion isolates with short incubation times respectively 263 K scrapie strain in hamsters, chronic wasting disease (CWD) strain CWD1 in bank voles and sheep PG127 scrapie in tg338 mice that are expressing sheep PrPVRQ.
Materials and methods
PrP-specific monoclonal antibodies (mAb’s) used were: SAF34, Bar224, 12B2, 9A2, 3F4, 6C2, 12F10, L42, 6H4, Sha31, SAF84, 94B4, F99/97.6.1 [30,31,32,33,34,35,36,37]. Their linear specificities on PrP have been described and further confirmed by Pepscan analysis  as follows (bovine PrP numbering, 6 octarepeats): 62QPHGGGW92 (SAF34), 101WGQGG105 (12B2), 110WNK112 (9A2), 117KTNMKHV113 (3F4), 122HVAGAAA128 (6C2), 152FGSDYEDRYYR162 (Bar224), 154 NDYEDRYYRE163 (12F10), 156YEDRYY161 (L42), 156YEDRYYRE163 (Sha31), 156YEDRYYREN164 (6H4), 174YRPVDQY180 (SAF84), 198HTVTTTTK205 (94B4) and 229YQRE232 (F99/97.6.1).
Bovine BSE brains were from the rostral part of the obex of a British clinically and histologically confirmed positive BSE cow (UK case 97/0913, kind gift from APHA Weybridge at UK), and obex tissues from confirmed BSE positive Dutch cases NL6 (clinically positive), NL11 and NL19 (clinically healthy at slaughter) as well as from BSE confirmed negative cattle. Hamster brains infected with the 263 K scrapie strain were supplied by RKI Berlin, sheep PG127 scrapie brain isolate was second oral passage material in VRQ/VRQ sheep prepared at ENVT Toulouse, CWD1 isolate was passaged three times in bank voles with PrP genotype 109I/I (Bv109I).
Animal experimentations were performed in 2004–2009 according to the prevailing regulations of European directives (86/609/EEC) as well as in compliance with the respective national and institutional legislations. The number of animals used were kept at the lowest as considered necessary for the experiments in line with the three R’s concept: replace, reduce and refine. This means per dose group 10–16 animals for Tgbov XV mice, and six for the other rodent bioassays.
Preparation of inocula
Brain materials from cattle and rodents were subjected to similar procedures with disposable equipment. Homogenizations were carried for 45 s at 23 000 rpm in Prypcon vials with a MediFASTH apparatus (Consul AR SA; Villeneuve, Switzerland). Negative tissues were first prepared before the positive ones, and dilutions of samples were performed with changing pipet tips for every next dilution step.
Brain stem material from cattle and sheep and rodent brains were homogenized as 10% (w/t) tissue samples either in physiological saline (PS) or in the presence of 2% sarkosyl as detergent in 50 mM sodium phosphate pH7.5.
The detergent containing homogenate was aliquoted. One aliquot was not heated. The other aliquot was placed in a 28 mL Bijou bottle covered with a paper fiber stopper and autoclaved in a pressure cooker at 115 °C for 40 min as described . One aliquot of the heated material was left undigested, and another part was further subjected to digestion for 60 min at 50 °C with 25–50 azocaseine-U KE mg−1 tissue equivalents (TE) unless otherwise stated. For the scrapie and CWD1 experiments an additional heated aliquot was digested with 0.015–0.005 mAnson-U PK mg−1 TE.
Undigested and digested homogenates were further diluted to 1% original tissue concentration in PS or, where mentioned, first precipitated with nine volumes of cold methanol by centrifugation for 10 min at 16 000 × g and resuspended in PS. After centrifugation of digested and heated material with or without methanol, a pellet was visible only when methanol was used. The 1% tissue homogenates were heated for 20 min at 80 °C and stored at −80 °C till use.
Tgbov XV mice overexpressing bovine PrP that are highly sensitive to bovine BSE infection were used for challenges by intracerebral inoculation with 20 μL of 1% (w/t) and lower doses tissue homogenate . Inocula were prepared (see paragraph below) from the British BSE case. End-point titers expressed as 10log infectious doses per g tissue (ID50 g−1) were determined applying the Spearman-Kaerber method .
Syrian hamsters, bank voles (Bv109I) and transgenic mice expressing the sheep PrPVRQ (Tg338) were used respectively for studies with 263 K scrapie infected hamster, CWD1109I from Bv109I after 3rd passage and sheep scrapie isolate PG127. Per animal 20 μL (50 μL in case of hamsters) of 1% (w/t) and lower doses of brain homogenate were intracerebrally inoculated as described [43,44,45]. Animals were culled when positive for clinical signs such as tremor of head or whole body, incoordination of gait, difficulty in rising from a supine position, and impairment in their capacity to feed. Animal brains were postmortem also checked for the presence of PrPres in Western blotting and in case of Tgbov XV mice also first by PrPres with TeSeE® SAP combination kit (Bio-Rad).
Infectivity titers were based on PrPSc detection in Tgbov XV mice and in the other three rodent lines on survival times.
Bovine brain homogenates were tested for presence of PrP using Western blotting as previously described . Running buffer was either 3-N-morpholino)propane sulfonic acid (MOPS) or 2-(N-morpholino) ethane sulfonic acid (MES). Staining of protein in gels was performed with the SilverXpress™ kit (Thermo Fisher Scientific) followed by destaining of silver and restaining with Coomassie brilliant blue [46, 47].
Brain material of Tgbov XV mice was tested for PrPres with the TeSeE® purification and detection kit (Biorad). Borderline and negative cases in TgBov XV mice were checked by Western blotting after a concentration procedure involving pelleting by ultracentrifugation at 540 000 × g . PrPres detection by Western blotting in hamsters with mAb 3F4, bank voles with mAb SAF84 and tg338-mice brain with mAb Sha31 followed described methods [43, 45, 48].
For further establishing presence of PrPSc or PrPres in BSE related samples, three different EC approved commercial enzyme-linked immunosorbent assays (ELISAs) for BSE testing were carried out: TeSeE® SAP combination kit (Bio-Rad), HerdCheck BSE Ag test (IDEXX Europe BV) and CediTect® BSE test (Prionics Lelystad BV). The general principle of these tests is described in Additional file 1.
Reference internal control samples were exactly treated as in routine testing is required. However, for each of the three tests, study samples were first precipitated with nine volumes of cold methanol, centrifuged at 16 000 × g for 10 min. The pellets containing 5.45 mg TE per vial were kept frozen until use. On day of analysis, pellets were taken up in the kit specific solution before including in the tests.
Pellets for the TeSeE kit were resuspended in denaturing kit buffer C and were heated as in the test kit for denaturation and subjected to the analysis in ELISA. Per well 3 mg TE were tested.
In the HerdCheck test, the pellets were resuspended in 600 μL test kit homogenization buffer and incubated at ambient temperature for 10 min. Then, 120 μL was mixed with 30 μL of plate diluent of which 100 μL was added per well of 96 wells IDEXX plate that contains Seprion ligand for binding PrPSc aggregates. Further denaturation to open bound aggregates for antibody binding was performed according to the kit protocol. Per well 0.7 mg TE were tested.
In the CediTect BSE test, pellets of the study samples were resuspended in 100 μL kit lysis buffer and after resuspension further ten times diluted with kit lysis buffer of which 50 μL (0.5 mg TE) was applied per well in each of two PVDF filter plates. Further procedure was according to the test protocol which means that after washing with phosphate buffered saline (PBS) by filtration, one plate was treated with PBS and the other with 5 M guanidinium thiocyanate in PBS and the other with PBS only. Per well 0.5 mg TE were tested.
PrPSc digestion in heat treated cattle brain
Heating alone in the presence of 2% sarkosyl at 115 °C of homogenates prepared from brain tissue of a British confirmed BSE cow with clinical signs did not lead to significant loss of PrPSc immunoreactivity (Figure 1B, cfr. lane 4 with lanes 1–3) as was previously also shown . Subsequent digestion with keratinase (KE) at increasing enzyme concentrations showed that all PrP material already disappeared at 5 KE-units per mg tissue equivalents (TE) (Figure 1B, lane 6). Staining with silver and Coomassie brilliant blue both showed that at this low enzyme/tissue ratio proteins were degraded to peptides migrating at 6 kDa and lower and to proteins with molecular masses of 300 kDa and higher (Figure 1 panels A1-2, lanes 6). However only in heated non-digested sample the ≥ 300 kDa fraction was reactive with PrP specific antibodies, but not after digestion with KE (Additional file 2 cfr. lane 4 with lane 7). This means that this large size protein material is accessible for PrP-specific antibodies. Precipitation with methanol and 1-propanol did show that the former treatment yielded acceptable recoveries of both PrP material and other proteins (Figure 1, panel A1, cfr. lane 1 with lanes 2 and 3). The level of PrP-reactive material was roughly the same between non-heated digested (lane 1) and heated non-digested or slightly digested homogenate (lanes 4 and 5).
In heated and KE-digested brain material, the extent of PrPSc removal by keratinase from high-titer central nervous system tissue of the BSE infected cow was above 99.9% (> factor 1000x) by Western blotting with mAb’s 98B4 and Sha31 (Figure 2). Other mAb’s yielded the same outcome such as SAF34, 9A2, 12B2, 6C2, 12F10, L42, SAF84 and F99/97.6.1. This infers that the destruction of PrP had involved the whole molecule.
Three different ELISA tests were used for quantifying the presence of PrPSc, each using a different property for immobilisation before further analysis, all using PrP specific mAb’s and each including a denaturation treatment to enable access of antibodies. These different properties were: 1 in TeSeE test the capture of PrPres by PrP specific antibody coated to polystyrene, 2 in the IDEXX HerdCheck test the binding of PrPSc-fibrils by Seprion ligand immobilized to polystyrene, and 3 in the Cedi-Tect BSE test the level of unfolding of PrPres—adsorbed to PVDF filters—which reflects its aggregated condition. In these tests PrPSc removal by keratinase in heated samples reached values 99.7–99.96% or alternatively PrPSc/PrPres reduction factors of > 2500x, > 333x, > 500x, and > 2500 × in respective TeSeE, HerdCheck, and Cedi with 9A2 and 94B4. In fact, each outcome was within borderline background (Table 1).
Taken together, from these three different biochemical analyses using a range of different PrP specific antibodies, it was not possible to show the presence of any left-over PrPSc in the heat-treated, keratinase digested, bovine brain.
Infectivity of BSE material in Tgbov XV mice
Infectivity of untreated cow brain homogenate used in the challenge experiments was estimated at 106.2–106.4 ID50 g−1, a value usual for BSE samples in mice transgenic for bovine PrP (Tgbov XV) [41, 49]. Heating at 115 °C led to an infectivity titer change down to 104.6 corresponding to a 40–60 fold titre loss (Table 2). A precipitation step with methanol applied on non-digested samples did increase titers 2- and fivefold for respectively non-heated and heated material which indicated that the precipitation of BSE agent was very effective. Surprisingly, subsequent proteolytic removal of PrPSc did not further remove infectivity, but it rather led to a 12.5-fold increase of infectivity (from 104.6 to 105.7). For confirmation of these results, we repeated these measurements with newly generated inoculum with dilutions around the critical doses 102.5 and 103.5. Results agreed with those in the previous experiment: digestion of heat-treated brain homogenate did not additionally remove infectivity but rather increased the infectivity (Figure 3). Thus, here a situation is encountered where removal of all detectable forms of PrP from prions did not further reduce infectivity (Additional file 3).
Moreover, in mice infected with these PrP depleted samples the triple band pattern of PrPres yielded a typical classical BSE strain profile with respect to migration position (e.g. lower band migrating at 19 kDa), a minimal 12B2 reactivity and the diglycosylated fraction (upper band) as the major PrP band similar to the sample used for challenge (Figure 4).
Infectivity of other TSE materials with short incubation times
To see whether retention of infectivity also would occur in similarly autoclaved TSEs from other sources we chose rapid infection models which were hamster 263 K scrapie in hamsters, sheep PG127 (or Dawson) scrapie in Tg338 shPrPVRQ mice, and bank vole CWD1109I in 109I/I bank voles (Bv109I) with reported minimal incubation times of less than 100 days. The effect of heat treatment and that of heat treatment plus digestion were checked by Western blotting which confirmed the effectiveness of the PrPSc removal by KE as well as proteinase K (Figure 5). The 10logID50 g−1 titers of non-heated inocula were for 263 K, PG127 scrapie and CWD1109I respectively 6–6.5, 5.8–6.3 and 8.4 (Table 3). However, heat treatment at 115 °C in each of the three systems led already to an infectivity reduction below the detection limit except maybe for bank voles where one out of six animals was positive at highest concentration tested corresponding to a titer of roughly 3.4 10logID50 g−1, which in that case would mean an infectivity reduction of at least 5 10log units. Subsequent digestion with KE or PK of all heated inocula yielded TSE negative outcomes for clinical signs and PrPres testing in Western blots.
These experiments do show that BSE differs from the other three TSEs with respect to resistance of infectivity to heat (i.c. 115 °C for 40 min under wet conditions and in the presence of detergent), a process that allowed proteolytic removal of PrPSc below the detection limits of the tests used.
In bovine BSE infected brain homogenate heated under wet conditions at 115 °C for 40 min a high level of infectivity was retained when inoculated in transgenic mice (Tgbov XV mice) expressing bovine PrP. This high level of residual heat resistant infectivity was not further inactivated by exhaustive proteolytic removal of PrPSc. In addition, the molecular BSE-strain type of PrPres appeared conserved in the mice. Similarly treated brain from three other prion sources with short incubation times in rodents lost their infectivity by the heat treatment, confirming the unique heat resistance of the BSE agent from cattle compared to that of other prions.
The loss of titre in bovine BSE brain homogenates after heating at 115 °C in the presence 2% sarkosyl amounted to only 0.7–1.8 10log ID50 g−1. Incomplete BSE inactivation tested in Tgbov XV mice compares well with other studies in homogenates using heating at temperatures between 100–140 °C for either bovine BSE in wild type mice and rodent BSE in transgenic mice expressing high levels of murine PrP [49,50,51]. The infectivity loss of scrapie types 263 K and PG127, and CWD1109I agrees with studies that BSE carries an exceptional resistance to wet heat conditions compared to many other TSE strains that get largely inactivated already below 100 °C [49, 52,53,54,55].
Remarkably, removal of PrPSc with keratinase after heating at 115 °C from BSE and other TSE strains did confirm the effectivity of the enzymatic digestion with keratinase (and proteinase K) when tested in Western blotting and, in case of BSE, in commercially available diagnostic tests. It may be that in the BSE material after heating and keratinase digestion some form(s) of PrPSc were preserved and remained undetectable. Protein material was found in both low molecular mass region < 6 kDa and in the > 300 kDa protein fraction, but both were not immunoreactive with PrP-specific antibodies. Also, additional testing for the presence of PrP using three different sensitive biochemical diagnostic ELISA’s did not reveal residual signs of PrPSc. Furthermore, an extra dissociation and unfolding treatment on Western blot PVDF membranes with guanidinium thiocyanate also did not lead to any binding of PrP specific antibody (not shown). If sub-background amounts of PrPSc or fragments thereof still were present in the inoculum, then the difference between infectivity reduction by heat (3–60 fold decrease) and PrPSc by subsequent breakdown with keratinase (333–2500 fold decrease) does not well correlate since these differences between the bioassay and biochemical data range between 5–800 fold. This was also observed by other studies with BSE, where infectivity and PrP-immunoreactivity cannot be simply compared in contrast to prion seeding assays like protein misfolding cyclic amplification (PMCA) and infectivity testing [49, 56]1. If PrP gets fully removed by our method—which is difficult to prove by the relative limited sensitivity of antibodies compared to infectivity testing in transgenic mice—other molecular entities should still be present that attribute to the PrPC to PrPSc conversion. Nevertheless, the discrepancy between infectivity and PrPSc reduction in our study and another study  is quite large which justifies searching for alternative factors or cofactors that promote PrPSc propagation depending on strain and environment. In fact, molecules like phospholipid, dextran sulfate and RNA have been reported to be involved in in vitro PrPSc prion formation and infectivity [4, 9, 18, 20, 49]. In this respect BSE represents a unique example of a prion agent that it is able to transmit to many other species and to induce PrP-PrPSc conversion within one species irrespective PrP-polymorphisms [57, 58].
The bovine BSE-typical molecular PrP triple band profile and molecular masses were retained in the brains of transgenic mice expressing bovine PrP when inoculated with heat and keratinase treated bovine BSE brain material. Also, the clinical signs observed were similar in the different inocula used. This corroborates observations about thermostability of strains and the hypothesis that the prion agent contains both a host (i.e. PrP) and a strain dependent component, the latter of which could be a non-protein component [24, 54].
Infectivity was not tested in KE-digested BSE samples that were not heated since we were focused on preparations where PrP was absent as an opportunity to produce a process for significant removal of BSE infectivity. Such test could have yielded information whether in case of BSE a proteolytic digestion of non-heated prion material had lead to infectivity reduction as is the case for e.g. with purified hamster scrapie material . Nevertheless, we did test for the presence of PrPSc in such samples by Western blots where the level of PrP-reactive material did hardly differ between non-heated digested and heated non-digested or partly-digested material, which suggests that infectivity also would have been retained in the non-heated material after digestion.
An approximately tenfold titre increase of infectivity was observed after keratinase treatment of the heated BSE samples which appeared reproducible in a second experiment (from 104.6 to 105.7 ID50 g−1, Table 2 and Figure 3). This increase leads us to some assumptions. Possibly there was still an undetectable amount of PrPSc present that under the conditions of the proteolytic treatment attained an increased PrP seeding capacity. Another more complicated possibility would be that after heating a strain determining factor for prion formation was released in the brain homogenate by the protease used. In that case, new PrPSc could be generated from PrP in the transgenic mouse brain leading to de novo infectivity while BSE strain properties remained the same. This latter situation also requires a complete conversion process leading to an infectivity titre higher than before proteolysis. The factor most probably is a macromolecular product still present in the pellet after methanol treatment.
The protein only theory has allowed to better understand the nature and origin of prion agents with PrPSc as the carrier of infectivity. As example of the validity of the prion hypothesis is the application of the one gene—one protein concept in the successful Mendelian way of breeding for resistance towards the disappearance of scrapie in sheep and observation of increased levels of a resistance related polymorphism in humans in the epidemic kuru region in Papua New Guinea [15, 16]. Yet, explaining the molecular basis of strains with their phenotypical behavior based on a polymorphic appearance of PrPSc remains a challenge. Here, the exceptional resistance of BSE to heat and the subsequent removal of PrPSc makes this prion type a rather unique substrate for solving these prion strain questions1. The answers could be found in the precipitable leftover material of the heated and digested BSE infected whole brain preparations. Thus, varying heat treatment and the enzymatic digestion conditions such as pH, detergent and choice of protease could well be of use in figuring out which additional molecular fraction can modulate the PrPC to PrPSc conversion towards strain related properties. Maybe other heat resistant strains than BSE could serve this aim with the potential practical advantage of absence of zoonotic behavior.
When in revision, a paper appeared where no PrPSc could be found in one out of three clinically positive steers orally infected with bovine BSE. In this one animal only by PMCA a weak positive signal for PrPSc was found in the 3rd round at highest seeding concentration while Tg BovXV mice remained negative for this steer. The two other steers were clearly PrPSc positive in biochemical tests, immunohistochemistry, PMCA at 1st, 2nd and 3rd round, and were infective in the bovinized mice .
Prusiner SB (1982) Novel proteinaceous infectious particles cause scrapie. Science 216:136–144
Brown P, Liberski PP, Wolff A, Gajdusek DC (1990) Conservation of infectivity in purified fibrillary extracts of scrapie-infected hamster brain after sequential enzymatic digestion or polyacrylamide gel electrophoresis. Proc Natl Acad Sci USA 87:7240–7244
Griffith JS (1967) Self-replication and scrapie. Nature 215:1043–1044
Ma J, Wang F (2014) Prion disease and the “protein-only hypothesis.” Essays Biochem 56:181–191
McKinley MP, Bolton DC, Prusiner SB (1983) A protease-resistant protein is a structural component of the scrapie prion. Cell 35:57–62
Avar M, Heinzer D, Steinke N, Dogancay B, Moos R, Lugan S, Cosenza C, Hornemann S, Andreoletti O, Aguzzi A (2020) Prion infection, transmission, and cytopathology modeled in a low-biohazard human cell line. Life Sci Alliance 3:e202000814
Bueler H, Aguzzi A, Sailer A, Greiner RA, Autenried P, Aguet M, Weissmann C (1993) Mice devoid of PrP are resistant to scrapie. Cell 73:1339–1347
Deleault NR, Harris BT, Rees JR, Supattapone S (2007) Formation of native prions from minimal components in vitro. Proc Natl Acad Sci U S A 104:9741–9746
Fernandez-Borges N, Di Bari MA, Erana H, Sanchez-Martin M, Pirisinu L, Parra B, Elezgarai SR, Vanni I, Lopez-Moreno R, Vaccari G, Venegas V, Charco JM, Gil D, Harrathi C, D’Agostino C, Agrimi U, Mayoral T, Requena JR, Nonno R, Castilla J (2018) Cofactors influence the biological properties of infectious recombinant prions. Acta Neuropathol 135:179–199
Kim JI, Cali I, Surewicz K, Kong Q, Raymond GJ, Atarashi R, Race B, Qing L, Gambetti P, Caughey B, Surewicz WK (2010) Mammalian prions generated from bacterially expressed prion protein in the absence of any mammalian cofactors. J Biol Chem 285:14083–14087
Makarava N, Kovacs GG, Bocharova O, Savtchenko R, Alexeeva I, Budka H, Rohwer RG, Baskakov IV (2010) Recombinant prion protein induces a new transmissible prion disease in wild-type animals. Acta Neuropathol 119:177–187
Salvesen O, Espenes A, Reiten MR, Vuong TT, Malachin G, Tran L, Andreoletti O, Olsaker I, Benestad SL, Tranulis MA, Ersdal C (2020) Goats naturally devoid of PrPC are resistant to scrapie. Vet Res 51:1
Wang F, Wang X, Yuan CG, Ma J (2020) Generating a prion with bacterially expressed recombinant prion protein. Science 327:1132–1135
Watts JC, Giles K, Stohr J, Oehler A, Bhardwaj S, Grillo SK, Patel S, DeArmond SJ, Prusiner SB (2012) Spontaneous generation of rapidly transmissible prions in transgenic mice expressing wild-type bank vole prion protein. Proc Natl Acad Sci U S A 109:3498–3503
Hagenaars TJ, Melchior MB, Windig JJ, Bossers A, Davidse A, van Zijderveld FG (2018) Modelling of strategies for genetic control of scrapie in sheep: the importance of population structure. PLoS One 13:e0195009
Mead S, Whitfield J, Poulter M, Shah P, Uphill J, Campbell T, Al-Dujaily H, Hummerich H, Beck J, Mein CA, Verzilli C, Whittaker J, Alpers MP, Collinge J (2009) A novel protective prion protein variant that colocalizes with kuru exposure. N Engl J Med 361:2056–2065
Ricci A, Allende A, Bolton D, Chemaly M, Davies R, Salvador Fernandez Escamez P, Girones R, Herman L, Koutsoumanis K, Lindqvist R, Nørrung B, Robertson L, Ru G, Sanaa M, Skandamis P, Speybroeck N, Simmons M, Ter Kuile B, Threlfall J, Wahlstrom H, Acutis P-L, Andreoletti O, Goldmann W, Langeveld J, Windig JJ, Ortiz Pelaez A, Snary E (2017) Genetic resistance to transmissible spongiform encephalopathies (TSE) in goats. EFSA J 15:4962
Deleault NR, Walsh DJ, Piro JR, Wang F, Wang X, Ma J, Rees JR, Supattapone S (2012) Cofactor molecules maintain infectious conformation and restrict strain properties in purified prions. Proc Natl Acad Sci U S A 109:E1938-1946
Hunter GD, Gibbons RA, Kimberlin RH, Millson GC (1969) Further studies of the infectivity and stability of extracts and homogenates derived from scrapie affected mouse brains. J Comp Pathol 79:101–108
Simoneau S, Thomzig A, Ruchoux MM, Vignier N, Daus ML, Poleggi A, Lebon P, Freire S, Durand V, Graziano S, Galeno R, Cardone F, Comoy E, Pocchiari M, Beekes M, Deslys JP, Fournier JG (2015) Synthetic scrapie infectivity: interaction between recombinant PrP and scrapie brain-derived RNA. Virulence 6:132–144
Lasmezas CI, Deslys JP, Robain O, Jaegly A, Beringue V, Peyrin J, Fournier JG, Hauw JJ, Rossier J, Dormont D (1997) Transmission of the BSE agent to mice in the absence of detectable abnormal prion protein. Science 275:402–405
Langeveld JP, Wang JJ, Van de Wiel DF, Shih GC, Garssen GJ, Bossers A, Shih JC (2003) Enzymatic degradation of prion protein in brain stem from infected cattle and sheep. J Infect Dis 188:1782–1789
Dickinson J, Murdoch H, Dennis MJ, Hall GA, Bott R, Crabb WD, Penet C, Sutton JM, Raven ND (2009) Decontamination of prion protein (BSE301V) using a genetically engineered protease. J Hosp Infect 72:65–70
Miyazawa K, Emmerling K, Manuelidis L (2011) High CJD infectivity remains after prion protein is destroyed. J Cell Biochem 112:3630–3637
Sklaviadis TK, Manuelidis L, Manuelidis EE (1989) Physical properties of the Creutzfeldt-Jakob disease agent. J Virol 63:1212–1222
Silveira JR, Raymond GJ, Hughson AG, Race RE, Sim VL, Hayes SF, Caughey B (2005) The most infectious prion protein particles. Nature 437:257–261
Colby DW, Prusiner SB (2011) Prions. In: Morimoto R, Kelly J, Selkoe D (eds), Cold Spring Harb Perspect Biol pp 1–23
Cronier S, Gros N, Tattum MH, Jackson GS, Clarke AR, Collinge J, Wadsworth JD (2008) Detection and characterization of proteinase K-sensitive disease-related prion protein with thermolysin. Biochem J 416:297–305
Leske H, Hornemann S, Herrmann US, Zhu C, Dametto P, Li B, Laferriere F, Polymenidou M, Pelczar P, Reimann RR, Schwarz P, Rushing EJ, Wuthrich K, Aguzzi A (2017) Protease resistance of infectious prions is suppressed by removal of a single atom in the cellular prion protein. PLoS One 12:e0170503
Demart S, Fournier JG, Creminon C, Frobert Y, Lamoury F, Marce D, Lasmezas C, Dormont D, Grassi J, Deslys JP (1999) New insight into abnormal prion protein using monoclonal antibodies. Biochem Biophys Res Commun 265:652–657
Feraudet C, Morel N, Simon S, Volland H, Frobert Y, Creminon C, Vilette D, Lehmann S, Grassi J (2005) Screening of 145 anti-PrP monoclonal antibodies for their capacity to inhibit PrPSc replication in infected cells. J Biol Chem 280:11247–11258
Harmeyer S, Pfaff E, Groschup MH (1989) Synthetic peptide vaccines yield monoclonal antibodies to cellular and pathological prion proteins of ruminants. J Gen Virol 79:937–945
Jacobs JG, Bossers A, Rezaei H, van Keulen LJ, McCutcheon S, Sklaviadis T, Lantier I, Berthon P, Lantier F, van Zijderveld FG, Langeveld JP (2011) Proteinase K-resistant material in ARR/VRQ sheep brain affected with classical scrapie is composed mainly of VRQ prion protein. J Virol 85:12537–12546
Jacobs JG, Langeveld JP, Biacabe AG, Acutis PL, Polak MP, Gavier-Widen D, Buschmann A, Caramelli M, Casalone C, Mazza M, Groschup M, Erkens JH, Davidse A, van Zijderveld FG, Baron T (2007) Molecular discrimination of atypical bovine spongiform encephalopathy strains from a geographical region spanning a wide area in Europe. J Clin Microbiol 45:1821–1829
Kascsak RJ, Rubenstein R, Merz PA, Tonna-DeMasi M, Fersko R, Carp RI, Wisniewski HM, Diringer H (1987) Mouse polyclonal and monoclonal antibody to scrapie-associated fibril proteins. J Virol 61:3688–3693
O’Rourke KI, Baszler TV, Besser TE, Miller JM, Cutlip RC, Wells GA, Ryder SJ, Parish SM, Hamir AN, Cockett NE, Jenny A, Knowles DP (2000) Preclinical diagnosis of scrapie by immunohistochemistry of third eyelid lymphoid tissue. J Clin Microbiol 38:3254–3259
Thuring CM, van Keulen LJ, Langeveld JP, Vromans ME, van Zijderveld FG, Sweeney T (2005) Immunohistochemical distinction between preclinical bovine spongiform encephalopathy and scrapie infection in sheep. J Comp Pathol 132:59–69
Slootstra JW, Puijk WC, Ligtvoet GJ, Langeveld JP, Meloen RH (1996) Structural aspects of antibody-antigen interaction revealed through small random peptide libraries. Mol Divers 1:87–96
Lin X, Lee CG, Casale ES, Shih JC (1992) Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl Environ Microbiol 58:3271–3275
Wang JJ, Shih J (1999) Fermentation production of keratinase from Bacillus licheniformis PWD-1 and a recombinant B. subtilis FDB-29. J Ind Microbiol Biotechnol 22:608–616
Buschmann A, Groschup MH (2005) Highly bovine spongiform encephalopathy-sensitive transgenic mice confirm the essential restriction of infectivity to the nervous system in clinically diseased cattle. J Infect Dis 192:934–942
Hubert JJ (1992) Bioassay. Kendall/Hunt Publishing Co, USA
Andreoletti O, Orge L, Benestad SL, Beringue V, Litaise C, Simon S, Le Dur A, Laude H, Simmons H, Lugan S, Corbiere F, Costes P, Morel N, Schelcher F, Lacroux C (2011) Atypical/Nor98 scrapie infectivity in sheep peripheral tissues. PLoS Pathog 7:e1001285
Beekes M, Baldauf E, Diringer H (1996) Sequential appearance and accumulation of pathognomonic markers in the central nervous system of hamsters orally infected with scrapie. J Gen Virol 77:1925–1934
Di Bari MA, Nonno R, Castilla J, D’Agostino C, Pirisinu L, Riccardi G, Conte M, Richt J, Kunkle R, Langeveld J, Vaccari G, Agrimi U (2013) Chronic wasting disease in bank voles: characterisation of the shortest incubation time model for prion diseases. PLoS Pathog 9:e100321
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Switzer RC 3rd, Merril CR, Shifrin S (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98:231–237
Thomzig A, Kratzel C, Lenz G, Kruger D, Beekes M (2003) Widespread PrPSc accumulation in muscles of hamsters orally infected with scrapie. EMBO Rep 4:530–533
Marin-Moreno A, Aguilar-Calvo P, Moudjou M, Espinosa JC, Beringue V, Torres JM (2019) Thermostability as a highly dependent prion strain feature. Sci Rep 9:11396
Schreuder BE, Geertsma RE, van Keulen LJ, van Asten JA, Enthoven P, Oberthur RC, de Koeijer AA, Osterhaus AD (1998) Studies on the efficacy of hyperbaric rendering procedures in inactivating bovine spongiform encephalopathy (BSE) and scrapie agents. Vet Rec 142:474–480
Taylor DM, Fraser H, McConnell I, Brown DA, Brown KL, Lamza KA, Smith GR (1994) Decontamination studies with the agents of bovine spongiform encephalopathy and scrapie. Arch Virol 139:313–326
Fernie K, Steele PJ, Taylor DM, Somerville RA (2007) Comparative studies on the thermostability of five strains of transmissible-spongiform-encephalopathy agent. Biotechnol Appl Biochem 47:175–183
Matsuura Y, Ishikawa Y, Murayama Y, Yokoyama T, Somerville RA, Kitamoto T, Mohri S (2020) Eliminating transmissibility of bovine spongiform encephalopathy by dry-heat treatment. J Gen Virol 101:136–142
Somerville RA, Gentles N (2011) Characterization of the effect of heat on agent strains of the transmissible spongiform encephalopathies. J Gen Virol 92:1738–1748
Taylor DM, Fernie K, Steele PJ, McConnell I, Somerville RA (2002) Thermostability of mouse-passaged BSE and scrapie is independent of host PrP genotype: implications for the nature of the causal agents. J Gen Virol 83:3199–3204
Ackermann I, Shawulu JC, Keller M, Fatola OI, Groschup MH, Balkema-Buschmann A (2018) Exploring PMCA as a potential in-vitro alternative method to mouse bioassays for the highly sensitive detection of BSE prions. Berl Münch Tierärztl Wochensch O/A-131:9/10, DOI https://doi.org/10.2376/0005-9366-18021
Priem J, Langeveld JP, van Keulen LJ, van Zijderveld FG, Andreoletti O, Bossers A (2014) Enhanced virulence of sheep-passaged bovine spongiform encephalopathy agent is revealed by decreased polymorphism barriers in prion protein conversion studies. J Virol 88:2903–2912
Torres JM, Espinosa JC, Aguilar-Calvo P, Herva ME, Relano-Gines A, Villa-Diaz A, Morales M, Parra B, Alamillo E, Brun A, Castilla J, Molina S, Hawkins SA, Andreoletti O (2014) Elements modulating the prion species barrier and its passage consequences. PLoS One 9:e89722
Dudas S, Anderson R, Staskevicus A, Mitchell G, Cross JC, Czub S (2021) Exploration of genetic factors resulting in abnormal disease in cattle experimentally challenged with bovine spongiform encephalopathy. Prion 15:1–11
APHA Weybridge at UK is thanked for kindly supplying the bovine BSE sample used for the infection testing. We are grateful for skillful technical assistance in hamster experiments by M. Joncic at RKI Berlin, in CediTest PrPSc BSE testing by J. Erkens at WBVR Lelystad, and in azo-caseine measurements by Dr J.J. Wang at North Carolina State University, USA.
Part of this study was presented at the international conference Prion 2007, September 26–28, 2007, at Edinburgh, UK.
The study on BSE was financially supported by the National Cattlemen’s and Beef Association (USA) Granted to Dr Jason Shih for period 2004–2006.
The authors declare that they have are no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1. Principle of the three ELISA tests to detect bovine PrP Sc and PrP res .
Sc digestion by keratinase of bovine brain after heat treatment at 115 °C. BSE infected brain in lanes 4, 7 and 10-13, negative control brain in lanes 2 and 3. Material in lanes 2 and 4 was heated at 115 °C in presence of detergent before digestion. Lanes 3 and 10-13: non-heated material digested by KE. In the heated BSE sample in lane 4 no PrP-specific immunoreactivity has remained neither throughout the lane nor in the high molecular mass region at the top, while in the non-heated material there was (lane 10). Lanes 1 and 9, mixture of molecular mass markers SeeBlue and MagicMark XP for which migration positions are indicated in kDa at the left; lanes 5, 6 and 8, no sample applied. Top and running front are indicated with arrow heads and arrows, respectively. Antibody used: 94B4 (0.2 μg mL-1). Gel was run in MOPS buffer. Symbols: TE = tissue equivalents; MM = molecular mass markers; KE = keratinase; + = treatment applied.
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Langeveld, J.P.M., Balkema-Buschmann, A., Becher, D. et al. Stability of BSE infectivity towards heat treatment even after proteolytic removal of prion protein. Vet Res 52, 59 (2021). https://doi.org/10.1186/s13567-021-00928-8
- Molecular mechanism