Figure 1

Digestion of total protein in BSE infected bovine brain homogenate. Panels A and B were derived from one SDS-PAGE gel, cut into two parts. Similar samples were loaded in the two parts before staining. Staining: A1, total protein with silver; A2, total protein with Coomassie brilliant blue after destaining the silver from A1; B, PrP staining after Western blotting using a mixture of antibodies 9A2 and 94B4 (each at 0.2 μg mL⁻¹). Heating in detergent containing homogenization buffer is indicated in the “115 °C” row. KE row shows the azocaseine-U mg⁻¹ tissue equivalents. Tissue equivalents applied: 250 μg per lane except for lane 4, only 62 μg to prevent overstaining. Samples in lanes 1 and 4–6 not precipitated, in lanes 2 and 3 precipitated respectively with methanol and 1-propanol. Molecular mass markers used were SeeBlue in A and B, and MagicMark XP in C, for which migration positions are indicated in kDa at the left. Gel was run in MES buffer. Top and running front are indicated with arrow heads and arrows, respectively.