PrPres patterns in diseased Tgbov XV mice brain inoculated with differently treated bovine BSE materials. All samples digested with PK. Panel A: two similar blots developed with different antibodies as indicated. Lane 1–5, inoculated Tg bovXV mice samples; lane 6, BSE positive bovine brain. Inocula used: lane 1, BSE in physiological saline; lanes 2–5, heated BSE homogenate of which lanes 2–3 not digested with KE and lanes 4–5 digested with KE; lanes 2 and 4, inocula precipitated in methanol. Panel B: blot of control material from Tgbov XV mice experimentally infected with either brain from a clinically affected and BSE confirmed cow (lane 3) or from a confirmed BSE negative cow (lane 2). Lanes 1 and 4, empty. Migration position of molecular mass markers are indicated at the left plus their size in kDa. Antibody concentration used for L42 and 12B2 was respectively 0.5 and 0.2 µg mL−1. Antibodies 9A2 and 94B4 yielded similar results as L42 (not shown).