Figure 5
From: Stability of BSE infectivity towards heat treatment even after proteolytic removal of prion protein
Proteolytic digestion of PrPSc in brain homogenates infected with PG127 scrapie, 263 K scrapie and CWD1. Heated and digested samples are indicated with an X. Three blots from left to right: sheep PG127, hamster 263 K and bank vole CWD1 immunostained with respectively Sha31, a mix of 3F4 and Sha31, and SAF84. Antibody concentrations 0.5 µg mL−1, except 3F4 at 1.0 µg mL−1. Enz-row shows where proteinase K or keratinase was used for digestion. N in lanes 2, 4, and 6 means TSE negative brain samples. Migration position of molecular mass markers are indicated at the left together with their kDa (SeeBlue markers). Gels were run in MOPS buffer. Tissue equivalents applied: 455 µg mL−1. Per panel lanes 1 and 2–6 are from the same blot.