- Research article
- Open Access
MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection
Veterinary Research volume 49, Article number: 62 (2018)
Peste des petits ruminants virus (PPRV) belongs to the genus Morbillivirus that causes an acute and highly contagious disease in goats and sheep. Virus infection can trigger the change in the cellular microRNA (miRNA) expression profile, which play important post-transcriptional regulatory roles in gene expression and can greatly influence viral replication and pathogenesis. Here, we employed deep sequencing technology to determine cellular miRNA expression profile in goat peripheral blood mononuclear cells (PBMC) infected with Nigeria 75/1 vaccine virus, a widely used vaccine strain for mass vaccination programs against Peste des petits ruminants. Expression analysis demonstrated that PPRV infection can elicit 316 significantly differentially expressed (DE) miRNA including 103 known and 213 novel miRNA candidates in infected PBMC at 24 hours post-infection (hpi) as compared with a mock control. Target prediction and functional analysis of these DEmiRNA revealed significant enrichment for several signaling pathways including TLR signaling pathways, PI3K-Akt, endocytosis, viral carcinogenesis, and JAK-STAT signaling pathways. This study provides a valuable basis for further investigation of the roles of miRNA in PPRV replication and pathogenesis.
Peste des petits ruminants (PPR) is an acute, highly contagious fatal disease in domestic and small wild ruminants, causing great economic losses in goat and sheep productivity . Peste des petits ruminants virus (PPRV), the causative agent of the disease, belongs to the genus Morbillivirus within the family Paramyxoviridae . Given its economic relevance and severity, PPR is classified as a world organization for animal health (OIE) listed disease [3, 4]. Based on phylogenetic analysis of partial “N” and “F” gene sequences, PPRV can be divided into four different lineages [3,4,5]. Live attenuated vaccines have already been used for control of PPR and showed a good immunological effect on both sheep and goats [6, 7]. Among these live attenuated vaccines, Nigeria 75/1 and Sungri/96 have been demonstrated to be safe and efficacious in conferring protection to sheep and goats against PPRV infection [7, 8]. PPRV is both lymphotropic and epitheliotropic in nature . PPRV enters lymphoid cells through the signaling lymphocyte activation molecule (SLAM), which is widely expressed on the surface of all immune cells . Peripheral blood mononuclear cells (PBMC) are widely used as a standard in vitro model to study host-PPRV interactions as in other morbillivirus infections [10,11,12,13]. Recently, transcriptome analysis of PBMC infected with PPRV uncovered transcription factors modulating immune responses [13, 14]. However, our understanding about the role of cellular microRNA (miRNA) during PPRV infection is still obscure.
MicroRNA are highly conserved small non-coding RNA of 19–24 nucleotides in length and play a critical role in many physiological and pathological processes such as development, proliferation, differentiation, apoptosis, immune response, and tumorigenesis in animals and plants [15,16,17]. Recently, a substantial number of research has implicated the role of miRNA in viral replication and has indicated they can inhibit or promote viral infection [18, 19]. Virus infection can trigger the changes in the cellular miRNA profile, which can greatly influence viral life cycles, viral tropism, and the pathogenesis of viral diseases [20,21,22,23].
In the current study, to explore the importance of miRNA regulation in PPRV infection, a deep sequencing approach was employed to identify differentially expressed miRNA in goat PBMC infected with PPRV Nigeria 75/1 vaccine virus. A bioinformatics analysis demonstrates that the differentially expressed miRNA play a crucial role in the PPRV-host cell interactions. The data suggest that host miRNA might have significant roles in modulating PPRV replication and pathogenesis.
Materials and methods
Ethics statement and experimental animals
The animal experiments were carried out in strict accordance with guidelines established by the Ethics of Animal Experiments of Northwest A&F University, Yangling, China. All the protocols were approved by this committee (Permit Number: 2014BAD23B11). Healthy 6-month-old goats used for blood collection were housed in appropriate containment facilities and had ad libitum access to feed and water. Goats were screened and shown negative for PPRV antibodies using competitive ELISA as well as a virus neutralization test.
PBMC isolation and virus infection
Goat PBMC were isolated using Histopaque-1077 (Sigma, USA) by density gradient centrifugation following the manufacturer’s instructions. Then, isolated cells from each goat were suspended into 70 mL RPMI-1640 medium (Hyclone, Logan, UT, USA) supplemented with 10% fetal calf serum (FCS), 2% l-glutamine, 100 mg/mL penicillin, and 100 IU/mL streptomycin. The PPRV vaccine strain, Nigeria 75/1, was obtained from the Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (Lanzhou, China). Virus stock was prepared by collecting the infected cell supernatant when cytopathic effect (CPE) affected about 80% of the cells. The virus was harvested by three cycles of freezing–thawing and stored at −80 °C and purified by banding on sucrose gradient. The purified virus titers were estimated by calculating 50% tissue culture infective doses (TCID50) using vero cells in 96 well microtitre plates. The purified virus was tested for its infectivity in vero cells and was used further for infection in goat PBMC.
For virus infection, goat PBMC were seeded into six well plates at a density of 1 × 105 cells/mL and were inoculated with Nigeria 75/1 at a multiplicity of infection (MOI) of 1.0. After 1 h of adsorption, infected cells were maintained in RPMI-1640 medium (Hyclone, Logan, UT, USA) containing 2% FCS, 2% l-glutamine and antibiotics. PBMC cells inoculated with similarly purified preparation from triple freezing–thawing vero cells were used as the mock-infected group. Viral infection in PBMC was confirmed with CPE and western blot. The CPE were observed under a light microscope at 0, 24, 48, and 72 hours post-inoculation (hpi). Western blot was performed using a polyclonal antibody against PPRV N protein to determine virus replication at the different time points. Three independent biological replicates of PPRV- and mock-inoculated cultures were prepared at each time point.
Western blot analysis
Protein homogenates from goat PBMC were extracted as previously described . Briefly, the cells were lysed for 20 min on ice in ice-cold lysis buffer (4906837001, Roche). The lysates were centrifuged at 12 000 × g for 20 min at 4 °C to obtain a clear lysate. The protein content of each sample was determined using the BCA Protein Assay Kit (Thermo Scientific). Then, equal amounts of protein were separated on a 12% SDS–polyacrylamide gel and transferred to polyvinylidene difluoride membranes. Membranes were probed overnight at 4 °C with anti-PPRV-N monoclonal antibody provided by China Animal Health and Epidemiology Center (Qingdao, China). All membranes were also probed with anti-β-actin antibody (MA1-744, Invitrogen). The bands were visualized using HRP-conjugated goat anti-mouse IgG (1:15 000, Boster) prior to the ECL protocol (Amersham Biosciences, Piscataway, NJ, USA). Changes in protein expression were determined after normalizing the band intensity of each lane to that of β-actin. Signal was visualized using the Konica SRX 101A developer (Konica Minolta Medical Imaging, Wayne, NJ, USA) and the Quantity One software (Bio-Rad, Mississauga, ON, Canada) was used for densitometrical analysis.
RNA isolation and small RNA library construction
PPRV-infected (n = 3) and mock-infected (n = 3) goat PBMC in six-well cell culture plates (6 × 105 cells/plate) were harvested at 24 hpi and washed three times with ice-cold phosphate buffered saline (PBS). Total RNA from PPRV-infected to mock-infected cells at 24 hpi was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. The total RNA quantity and concentration of both samples were measured with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) and the NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). An RNA integrity number score ≥ 8 and rRNA 28S/18S ≥ 1.6 were required in the study. Two groups of total RNA were used for library preparation and sequencing by pooling equal quantities (10 µg) of total RNA isolated from three individual PPRV-infected and mock-infected goat PBMC. Briefly, total RNA were purified by polyacrylamide gel electrophoresis (PAGE) to enrich 15–35 nt molecules, then proprietary adapters were ligated to the 5′ and 3′ terminal of the RNA and the samples were used as templates for cDNA synthesis. The cDNA was amplified using the appropriate number of PCR cycles to produce sequencing libraries, which were subsequently subjected to the proprietary Solexa sequencing-by-synthesis method using the Illumina Genome Analyzer (SanDiego, CA, USA). Sequencing was carried out at the BGI Bio-tech Inc. (Guangzhou, China).
RNA sequencing and data processing
The various steps followed in the present study are summarized in Additional file 1. Raw sequencing reads from each library were first cleaned by removing the low-quality tags and adapter sequences. The reads between 18 and 30 nt were screened against the GenBank database and Rfam (10.1) database  to remove rRNA, tRNA, small nucleolar RNA, small nuclear RNA, repeats, exon and intron sequences. The clean reads were mapped to the capra hircus genome , and their expression and distribution patterns were analyzed using the Short Oligonucleotide Alignment Program (SOAP) . The sRNA were aligned to the miRNA precursors of the reference species (Capra hircus) in the Sanger miRBase 21.0 database to identify known miRNA, as well as the base bias for the first position of the identified miRNA with a certain length and for each position of all of the identified miRNA. By comparing our sequences with those in the databases, sRNA can be annotated into different categories. After excluding sRNA that were identified by the above categories, the unannotated short reads were subjected to novel miRNA prediction using the miReap program. Differential expression analysis between PPRV-infected and mock-infected cells of the two samples was performed using the DEGseq R package. A P value < 0.01 and a │log2 (fold change) │ > 1 were set as the default thresholds for significant differential expression.
miRNA target identification and gene ontology analysis
The target genes for each differentially expressed miRNA were predicted using the miRanda and RNAhybrid algorithms. The predicted target genes were then subjected to gene ontology (GO) functional analysis utilizing the WEGO program to create histograms of GO annotation against cell components, biological processes, and molecular functions . To further explore the biological function of the predicted target genes, a Kyoto Encyclopedia of Genes Genomes (KEGG) pathways enrichment analysis was performed using the KOBAS 2.0 annotation tool.
Quantitative real-time RT-PCR
To validate the sequencing data, we randomly selected nine differentially expressed miRNA for quantitative real-time RT-PCR analysis using Bulge-loop™ miRNA qRT-PCR Primer Sets (one RT primer and a pair of qPCR primers for each set) and performed on ABI 7500 System (Applied Biosystems, Warrington, UK). The primers for selected miRNA and internal standard 5S snRNA are designed by RiboBio Inc. (GuangZhou. China) and the sequences are covered by a patent. Briefly, 10 µg of cDNA was added to 10 µL of the 2 × SYBR green PCR master mix with 0.2 µL of Taq polymerase enzyme (200 U/µL, RiboBio, China), 200 nM of each primer and ddH2O to a final volume of 20 µL. The reactions were amplified for 10 s at 95 °C and 20 s at 60 °C for 40 cycles. The thermal denaturation protocol was run at the end of the PCR to determine the number of products that were presented in the reaction mix. Reactions were typically run in duplicate. The gene 5S was used as the internal reference gene. The relative expression level of each miRNA was calculated using the 2−ΔΔCt method .
Characterization of PPRV replication in goat PBMC
To determine the kinetics of PPRV replication in goat PBMC, CPE and viral N protein expression were all detected at 0, 24, 48 and 72 hpi. Compared to mock-infected cells, CPE of the PPRV-infected cells exhibited ballooning and clumping at 24 hpi (Figure 1A). From 48 to 72 hpi, almost all the PPRV-infected cells show swelling or granular degeneration as presented in the Figure 1A. Western blot analysis revealed that significant expression levels of N protein were detected as early as 24 hpi in PPRV-infected goat PBMC (Figure 1B). In general, no excessive CPE but with high virus levels is regarded as the optimal time for miRNA expression analysis in host cells during early stage virus infection, because it may trigger global and strong antiviral immune responses [18, 20, 22]. Here PPRV- and mock-infected cells were harvested at 24 hpi in triplicates for further miRNA sequencing analysis to guarantee a higher proportion of infected cells and to avoid excessive CPE.
Construction and sequencing data analysis of small RNA libraries
To identify miRNA changes in goat PBMC infected with PPRV, two small RNA libraries pooled from mock-infected to PPRV-infected groups were constructed at 24 hpi. We performed high-throughput small RNA sequencing on small RNA libraries obtained from mock- to PPRV-infected goat PBMC. In total, 33 933 810 and 33 852 635 raw reads were obtained from the uninfected and infected groups, respectively (Additional file 2). After removing low-quality sequences, adapter sequences, and sequences smaller than 18 nt, 30 573 869 and 30 644 798 clean reads were identified in the mock-infected and PPRV-infected groups, respectively. A total of 29 487 110 (mock-infected) and 26 296 606 (PPRV-infected) clean reads were perfectly mapped to the goat hircus genome [26, 30]. All of the clean reads were annotated and classified as snRNA, rRNA, snoRNA, Rfam other sncRNA, precursor miRNA, mature miRNA, intergenic, intron, exon, and repeats (Additional file 2). Data show that the length distributions of the small RNA from both libraries were 21–23 nt (Figure 2), which was consistent with the typical size of mature miRNA from Dicer-derived products . These results indicate that miRNA have been enriched successively from both libraries.
Different expression analysis of miRNA
To identify known miRNA in goat PBMC, we aligned the small RNA from our libraries to the known mature miRNA and their precursors of the reference species (Capra hircus) in the Sanger miRBase 21.0 database using BLASTN searches to obtain the miRNA count as well as the base bias at the first position. As shown in Figure 3, 459 of 647 unique miRNA genes were co-expressed in both libraries, and 25 and 163 of these miRNA appeared to be preferentially expressed in the PPRV-infected and mock-infected PBMC cell libraries, respectively. Using a P-value < 0.01 and a│log2 (fold change)│ > 1 as the cut-off values, a total of 316 miRNA (including 103 known miRNA and 213 novel miRNA) were differentially expressed in the two groups (Additional file 3). Among these 316 DEmiRNA, 147 miRNA were upregulated and 169 miRNA were downregulated in the PPRV-infected cells compared to the mock-infected cells (Figure 3 and Additional file 3).
Target gene prediction for miRNA
To understand the molecular function and biological processes of miRNA during PPRV infection, two independent algorithms, miRanda and RNAhybrid, were used to predict the mRNA targets for each of the miRNA that was differentially expressed. 12 065 target genes for 103 known miRNA and 213 novel miRNA differentially expressed in the mock-infected and PPRV-infected groups were predicted (Additional file 4). Among these 316 differentially expressed (DE) miRNA co-expressed in the two groups identified, 15 miRNA were identified based on fold change values, and the role of their putative target genes in pro viral and antiviral response was identified (Table 1). Among these 15 DEmiRNA identified, ten miRNA were found to be upregulated while another five miRNA were downregulated in PPRV-infected cells. Particularly, most of the genes targeted by these DEmiRNA have profound immune evasive functions.
Gene ontology and KEGG pathway analysis of target genes
WEGO analysis shows that a total of 12 065 target genes were successfully annotated for 103 known miRNA and 213 novel miRNA differentially expressed in two groups. The annotated target genes were mainly involved in immune system processes, response to stimulus, metabolic process, binding, organelle and cell processes (Figure 4, Additional file 5). To analyze the roles that these miRNA might play in regulatory networks, we also assigned the putative miRNA targets to the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways using the KEGG Orthology Based Annotation System (KOBAS). KEGG pathway annotation revealed that 10 364 background genes were annotated for 317 biological processes such as metabolic pathways (ko01100), MAPK signaling pathway (ko04010), PI3K-Akt signaling pathway (ko04151), endocytosis (ko04144), Hippo signaling pathway (ko04390), NF-κB signaling pathway (ko04064), viral carcinogenesis (ko05203), FoxO signaling pathway (ko04068), Jak-STAT signaling pathway (ko04630) and Toll-like receptor signaling pathway (ko04620) (Additional file 6). The results indicate that the differentially expressed miRNA may play a crucial role in the virus-host interactions.
Validation of deep sequencing results by quantitative RT-PCR
Quantitative RT-PCR was performed to validate the deep sequencing data of nine selected differentially expressed miRNA, including six known miRNA and three novel miRNA. Collectively, the relative expression levels of the selected miRNA detected with qRT-PCR were consistent with that determined with the deep sequencing results. The results confirmed the upregulation of two miRNA expression (chi-miR-204-3p, novel-mir218) and the downregulation of seven miRNA (chi-miR-338-3p, chi-miR-30b-3p, chi-miR-199a-5p, chi-miR-199a-3p, chi-miR-1, novel-mir330, novel-mir208) in mock-infected goat PBMC compared with the PPRV-infected cells (Figure 5).
Deep sequencing has provided a powerful tool to identify differentially expressed miRNA, especially low abundance ones, under conditions of physiological perturbation. Some miRNA can inhibit virus replication by regulating host immune responses or targeting viruses, whereas other miRNA can also promote viral replication by modulating the cellular environment [22, 32, 33]. Although many miRNA have been reported to be key regulators of virus-host interaction, to our knowledge, host miRNA expression profile in goat PBMC affected by PPRV infection has not been investigated. PBMC play an important role in pathogen recognition and induction of early immune response [10, 13, 14]. In the present study, we identified differentially expressed miRNA in goat PBMC in response to PPRV infection. We first determined the morphological changes taking place in cells in succession cells following PPRV infection and analyzed viral replication in PPRV-infected PBMC. Compared to mock-infected cells, significant CPE was observed in PPRV-infected PBMC at 24 hpi, and most cells show swelling or granular degeneration from 48 to 72 hpi. In concordance with high PPRV production detected at 24 hpi by Western blot assay, we chose 24 hpi as the time point to measure the miRNA expression profiles, because it might trigger global and strong antiviral immune responses, and avoid excessive CPE in the PPRV-infected PBMC cells. Detailed analysis revealed many differences in the global expression profile of miRNA among mock- and PPRV-infected goat PBMC. A total of 316 differently expressed miRNA were identified in PPRV-infected groups compared to the mock-infected group.
Individual miRNA have the potential to regulate the expression of multiple mRNA, and the changes in miRNA expression following viral infection are predicted to have profound effects on the host responses [34,35,36]. In order to evaluate the potential biological roles of DEmiRNA expressed in goat PBMC in response to PPRV infection, we predicated the potential target genes for the DEmiRNA identified in our miRNA sequencing datasets. A total of 12 065 target genes were annotated for 103 known miRNA and 213 novel miRNA differentially expressed both in mock- and PPRV-infected groups. Using the predicted target genes, the GO enrichment analysis revealed that these differentially expressed genes were functionally involved in responses to stimuli, immune system processes, biological regulation, and other cellular processes. The host innate immune system is stimulated once the TLR are engaged with pathogen associated molecular patterns (PAMP) . Previous studies have shown that mRNA levels of TLR3, 7/10 of goat PBMC are upregulated by PPRV vaccine virus Sungri/96 strain infection and these control both the sensing and the proinflammatory and antiviral responses to PPRV [13, 14]. The KEGG pathway analysis in our study further determined that most genes targeted by DEmiRNA were mainly involved in important cellular pathways, including MAPK, PI3K-Akt, endocytosis, Hippo, NF-κB, viral carcinogenesis, FoxO, Jak-STAT and Toll-like receptor signaling pathways. All these functional analyses suggest that, by regulating the target genes, cellular miRNA have a profound effect on the regulation of innate immunity of goat PBMC to PPRV early infection.
Identification of differentially expressed host miRNA is just the first step towards understanding miRNA regulation of host-virus interactions. Then we must identify how the underlying mechanisms that are targeted by differentially expressed miRNA promote or inhibit PPRV replication. Dissection of miRNA modulation during PPRV infection will provide insight into the cellular mechanisms of host-virus interaction. The innate immune response is the first line of defence against viruses [38,39,40]. Emerging data have identified an important contribution of miRNA to the regulation of innate immune systems [41, 42]. Here, PPRV infection in goat PBMC triggered the expression of many immune-related miRNA, including miR-150, miR-146, and let-7 family as previously observed in spleen and lung tissues of goats infected with PPRV . Furthermore, 15 miRNA were identified from the 316 DEmiRNA co-expressed in two groups based on fold change values and their targeted gene roles in viral infection, antiviral response and apoptosis. Interferon (IFN)-mediated pathway is a crucial part of the cellular response against viral infection . It is known that most of the Morbillivruses antagonize the production of Type I IFN [45,46,47]. There is evidence that PPRV accessory proteins (V and C) are involved in controlling the host’s interferon responses [45, 46]. However, the latest research indicates that PPRV has a V-independent mechanism for actively inhibiting IFN-β induction in goat non-immune cells infected with wild-type and vaccine strains of PPRV . Although an important role of PPRV protein interaction with host protein in evasion of IFN-induced antivirus effects has been identified [46, 47], little is known about the mechanisms for miRNA regulation of the suppression of IFN induction in PPRV infection. In this study, we found that a member of the interferon regulatory transcription factor IRF3 and interferon alpha/beta receptor IFNAR1 were annotated as gene targets by upregulated miR, chi-miR-323a-3p and chi-miR-1291, respectively. IRF3 and IRF7 play an essential role in the induction of Type I IFN (IFN-α/β). It has been reported that IFN-α/β mRNA were not detected in goat PBMC in response to Sungri/96 PPRV vaccine virus infection . It is important to note that, TMEM173, a major stimulator in IFN production, was targeted by three downregulated DEmiRNA, including chi-miR-1, chi-miR-30b-3p and novel_mir3, and by two upregulated chi-miR-1291 and chi-miR-671-5p in PPRV-infected cells. This may give a new insight into how the post-transcriptional gene regulation mechanisms of PPRV escape IFN signaling.
Previous studies indicated that PPRV infection in goat PBMC can induce a classic inflammatory response characterized by enhanced expression of transcription of cytokines such as TNFα, IL-18, IL-12, IFNγ, IFNβ, as well as anti-inflammatory cytokines, including IL-4 and IL-10 . In this study, it was found that tumor necrosis factor receptor superfamily member TNFRSF9 genes were targeted by chi-miR-1, chi-miR-1291, novel_mir3, while tumor necrosis factor ligand superfamily member TNFSF13B genes were targeted by novel_mir1, novel_mir85 and novel_mir70. Furthermore, it has been previously demonstrated that miRNA play an important role in regulating NF-κB signaling pathway during viral infections  and activation of NF-κB . Our study revealed that NF-κB signaling-related molecules NFKB2 and NFKBIA were targeted by novel_mir3 and chi-miR-155-3p in PPRV infected goat PBMC, respectively.
There is evidence demonstrating that in vitro infection of goat PBMC with PPRV caused apoptosis [51, 52]. Particularly, most of the genes targeted by DEmiRNA in this study are involved in the regulation of cellular apoptosis. In this study, we found that PPRV infection triggered several DEmiRNA, including chi-miR-182, chi-miR-671-5p, novel_mir3, and novel_mir330, that target apoptosis-related genes AREL1, CASP8, APAF1, and BAX in goat PBMC. Henceforth by regulating the target genes, cellular miRNA have a profound effect on the regulation of apoptosis of goat PBMC to PPRV early infection. Further investigations are needed to clarify the underlying mechanisms on how these genes are targeted by differentially expressed miRNA and their role in promoting or inhibiting PPRV replication. Although numerous studies demonstrate that PPRV can induce apoptotic cell death in vitro, there is no evidence for in vivo host cell apoptosis. The reasons may be associated with the different expression levels of cellular receptor, infection route, strain-specific and virus infection dose between in vitro and in vivo studies.
In summary, we identified miRNA and evaluated their expression patterns in goat PBMC in response to PPRV infection using deep sequencing. This study supports previous studies indicating the importance of the miRNA landscape in the replication and pathogenesis of PPRV. One-hundred and three known differentially expressed miRNA and 213 novel miRNA candidates were identified. The target genes of these differential miRNA were estimated by GO enrichment and KEGG pathway analyses. In addition, while these data demonstrate the differentially expressed miRNA profiles in goat PBMC in response to vaccine strain of PPRV infection, more studies are needed to understand how wild-type PPRV infection affect miRNA expression patterns.
Banyard AC, Parida S, Batten C, Oura C, Kwiatek O, Libeau G (2010) Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control. J Gen Virol 91:2885–2897
Gibbs EP, Taylor WP, Lawman MJ, Bryant J (1979) Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus. Intervirology 11:268–274
Kumar KS, Babu A, Sundarapandian G, Roy P, Thangavelu A, Kumar KS, Arumugam R, Chandran ND, Muniraju M, Mahapatra M, Banyard AC, Manohar BM, Parida S (2014) Molecular characterisation of lineage IV peste des petits ruminants virus using multi gene sequence data. Vet Microbiol 174:39–49
Kumar N, Maherchandani S, Kashyap SK, Singh SV, Sharma S, Chaubey KK, Ly H (2014) Peste des petits ruminants virus infection of small ruminants: a comprehensive review. Viruses 6:2287–2327
Albina E, Kwiatek O, Minet C, Lancelot R, Servan de Almeida R, Libeau G (2013) Peste des petits ruminants, the next eradicated animal disease? Vet Microbiol 165:38–44
Diallo A, Minet C, Le Goff C, Berhe G, Albina E, Libeau G, Barrett T (2007) The threat of peste des petits ruminants: progress in vaccine development for disease control. Vaccine 25:5591–5597
Saravanan P, Sen A, Balamurugan V, Rajak KK, Bhanuprakash V, Palaniswami KS, Nachimuthu K, Thangavelu A, Dhinakarraj G, Hegde R, Singh RK (2010) Comparative efficacy of peste des petits ruminants (PPR) vaccines. Biologicals 38:479–485
Diallo A, Barrett T, Barbron M, Subbarao SM, Taylor WP (1989) Differentiation of rinder pest and peste des petits ruminants viruses using specific cDNA clones. J Virol Methods 23:127–136
Pawar RM, Dhinakar Raj G, Balachandran C (2008) Relationship between thelevel of signaling lymphocyte activation molecule mRNA and replication of Peste-des-petits-ruminants virus in peripheral blood mononuclear cells of host animals. Acta Virol 52:231–236
Bolt G, Berg K, Blixenkrone-Moller M (2002) Measles virus-induced modulation of host-cell gene expression. J Gen Virol 83:1157–1165
Iwasa T, Suga S, Qi L, Komada Y (2010) Apoptosis of human peripheral blood mononuclear cells by wild-type measles virus infection is induced by interaction of hemagglutinin protein and cellular receptor, SLAM viacaspase-dependent pathway. Microbiol Immunol 54:405–416
Dhanasekaran S, Biswas M, Vignesh AR, Ramya R, Raj GD, Tirumurugaan KG, Raja A, Kataria RS, Parida S, Subbiah E (2014) Toll-like receptor responses to Peste des petits ruminants virus in goats and water buffalo. PLoS One 9:e111609
Manjunath S, Kumar GR, Mishra BP, Mishra B, Sahoo AP, Joshi CG, Tiwari AK, Rajak KK, Janga SC (2015) Genomic analysis of host–Peste des petits ruminants vaccine viral transcriptome uncovers transcription factors modulating immune regulatory pathways. Vet Res 46:15
Manjunath S, Mishra BP, Mishra B, Sahoo AP, Tiwari AK, Rajak KK, Muthuchelvan D, Saxena S, Santra L, Sahu AR, Wani SA, Singh RP, Singh YP, Pandey A, Kanchan S, Singh RK, Kumar GR, Janga SC (2017) Comparative and temporal transcriptome analysis of peste des petits ruminants virus infected goat peripheral blood mononuclear cells. Virus Res 229:28–40
Axtell MJ, Westholm JO, Lai EC (2011) Vive la difference: biogenesis and evolution of microRNAs in plants and animals. Genome Biol 12:221
Bartel DP (2009) MicroRNAs: target recognition and regulatory functions. Cell 136:215–233
Shukla GC, Singh J, Barik S (2011) MicroRNAs: processing, maturation, target recognition and regulatory functions. Mol Cell Pharmacol 3:83–92
Haasnoot J, Berkhout B (2011) RNAi and cellular miRNAs in infections bymammalian viruses. Methods Mol Biol 721:23–41
Sharma N, Singh SK (2016) Implications of non-coding RNAs in viral infections. Rev Med Virol 26:356–368
Shrinet J, Jain S, Jain J, Bhatnagar RK, Sunil S (2014) Next generation sequencing reveals regulation of distinct Aedes microRNAs during chikungunya virus development. PLoS Negl Trop Dis 8:e2616
Skovgaard K, Cirera S, Vasby D, Podolska A, Breum SO, Durrwald R, Schlegel M, Heegaard PM (2013) Expression of innate immune genes, proteins and microRNAs in lung tissue of pigs infected experimentally with influenza virus (H1N2). Innate Immun 19:531–544
Slonchak A, Hussain M, Torres S, Asgari S, Khromykh AA (2014) Expression of mosquito microRNA Aae-miR-2940-5p is downregulated in response to West Nile virus infection to restrict viral replication. J Virol 88:8457–8467
Zhang Y, Jing J, Li X, Wang J, Feng X, Cao R, Chen P (2015) Integration analysis of miRNA and mRNA expression profiles in swine testis cells infected with Japanese encephalitis virus. Infect Genet Evol 32:342–347
Qi X, Qin L, Du R, Chen Y, Lei M, Deng M, Wang J (2017) Lipopolysaccharide upregulated intestinal epithelial cell expression of Fn14 and activation of Fn14 signaling amplify intestinal TLR4-mediated inflammation. Front Cell Infect Microbiol 7:315
Nawrocki EP, Burge SW, Bateman A, Daub J, Eberhardt RY, Eddy SR, Floden EW, Gardner PP, Jones TA, Tate J, Finn RD (2015) Rfam 12.0: updates to the RNA families database. Nucleic Acids Res 43:D130–D137
Dong Y, Xie M, Jiang Y, Xiao N, Du X, Zhang W, Tosser-Klopp G, Wang J, Yang S, Liang J, Chen W, Chen J, Zeng P, Hou Y, Bian C, Pan S, Li Y, Liu X, Wang W, Servin B, Sayre B, Zhu B, Sweeney D, Moore R, Nie W, Shen Y, Zhao R, Zhang G, Li J, Faraut T, Womack J, Zhang Y, Kijas J, Cockett N, Xu X, Zhao S, Wang J, Wang W (2013) Sequencing and automated whole-genome optical mapping of the genome of a domestic goat (Capra hircus). Nat Biotechnol 31:135–141
Li R, Li Y, Kristiansen K, Wang J (2008) SOAP: short oligonucleotide alignment program. Bioinformatics 24:713–714
Ye J, Fang L, Zheng H, Zhang Y, Chen J, Zhang Z, Wang J, Li S, Li R, Bolund L (2006) WEGO: a web tool for plotting GO annotations. Nucleic Acids Res 34:W293–W297
Schmittgen TD, Lee EJ, Jiang J, Sarkar A, Yang L, Elton TS, Chen C (2008) Real-time PCR quantification of precursor and mature microRNA. Methods 44:31–38
Lai F, Zhai H, Cheng M, Ma J, Cheng S, Ge W, Zhang G, Wang J, Zhang R, Wang X, Min L, Song J, Shen W (2016) Whole-genome scanning for the litter size trait associated genes and SNPs under selection in dairy goat (Capra hircus). Sci Rep 6:38096
Liu Y, Zhou Y, Wu J, Zheng P, Li Y, Zheng X, Puthiyakunnon S, Tu Z, Chen XG (2015) The expression profile of Aedes albopictusmiRNAs is altered by dengue virus serotype- 2 infection. Cell Biosci 5:16
Jopling CL, Yi M, Lancaster AM, Lemon SM, Sarnow P (2005) Modulation of hepatitis C virus RNA abundance by a liver-specific MicroRNA. Science 309:1577–1581
Norman KL, Sarnow P (2010) Modulation of hepatitis C virus RNA abundance and the isoprenoid biosynthesis pathway by microRNA miR-122 involves distinct mechanisms. J Virol 84:666–670
Ghosh Z, Mallick B, Chakrabarti J (2009) Cellular versus viral microRNAs in host-virus interaction. Nucleic Acids Res 37:1035–1048
Lu LF, Gasteiger G, Yu IS, Chaudhry A, Hsin JP, Lu Y, Bos PD, Lin LL, Zawislak CL, Cho S, Sun JC, Leslie CS, Lin SW, Rudensky AY (2015) A singlemiRNA-mRNA interaction affects the immune response in a context-and cell-type-specific manner. Immunity 43:52–64
Samir M, Vaas LA, Pessler F (2016) MicroRNAs in the host response to viral infections of veterinary importance. Front Vet Sci 3:86
Girardin SE, Sansonetti PJ, Philpott DJ (2002) Intracellular vs extracellular recognition of pathogens—common concepts in mammals and flies. Trends Microbiol 10:193–199
Gack MU, Diamond MS (2016) Innate immune escape by Dengue and West Nile viruses. Curr Opin Virol 20:119–128
Schwartz-Cornil I, Mertens PP, Contreras V, Hemati B, Pascale F, Breard E, Mellor PS, MacLachlan NJ, Zientara S (2008) Bluetongue virus: virology, pathogenesis and immunity. Vet Res 39:46
Vitour D, Doceul V, Ruscanu S, Chauveau E, Schwartz-Cornil I, Zientara S (2014) Induction and control of the type I interferon pathway by Bluetongue virus. Virus Res 182:59–70
Johnnidis JB, Harris MH, Wheeler RT, Stehling-Sun S, Lam MH, Kirak O, Brummelkamp TR, Fleming MD, Camargo FD (2008) Regulation of progenitor cell proliferation and granulocyte function by microRNA-223. Nature 451:1125–1129
Liu G, Friggeri A, Yang Y, Park YJ, Tsuruta Y, Abraham E (2009) miR-147, a microRNA that is induced upon Toll-like receptor stimulation, regulates murine macrophage inflammatory responses. Proc Natl Acad Sci U S A 106:15819–15824
Pandey A, Sahu AR, Wani SA, Saxena S, Kanchan S, Sah V, Rajak KK, Khanduri A, Sahoo AP, Tiwari AK, Mishra B, Muthuchelvan D, Mishra BP, Singh RK, Gandham RK (2017) Modulation of host miRNAs transcriptome in lung and spleen of Peste des petits ruminants virus infected sheep and goats. Front Microbiol 8:1146
Wu J, Shen L, Chen J, Xu H, Mao L (2015) The role of microRNAs in enteroviral infections. Braz J Infect Dis 19:510–516
Chinnakannan SK, Nanda SK, Baron MD (2013) Morbillivirus v proteins exhibit multiple mechanisms to block type 1 and type 2 interferon signalling pathways. PLoS One 8:e57063
Ma X, Yang X, Nian X, Zhang Z, Dou Y, Zhang X, Luo X, Su J, Zhu Q, Cai X (2015) Identification of amino-acid residues in the V protein of peste des petits ruminants essential for interference and suppression of STAT-mediated interferon signaling. Virology 483:54–63
Sanz Bernardo B, Goodbourn S, Baron MD (2017) Control of the induction of type I interferon by Peste des petits ruminants virus. PLoS One 12:e0177300
Baron J, Bin-Tarif A, Herbert R, Frost L, Taylor G, Baron MD (2014) Early changes in cytokine expression in peste des petits ruminants disease. Vet Res 45:22–33
Gao Z, Dou Y, Chen Y, Zheng Y (2014) MicroRNA roles in the NF- kappaB signaling pathway during viral infections. Biomed Res Int 2014:436097
Hoesel B, Schmid JA (2013) The complexity of NF-kappaB signaling in inflammation and cancer. Mol Cancer 12:86
Mondal B, Sreenivasa BP, Dhar P, Singh RP, Bandyopadhyay SK (2001) Apoptosis induced by peste des petits ruminants virus in goat peripheral blood mononuclear cells. Virus Res 73:113–119
Zhang Y, Wu S, Lv J, Feng C, Deng J, Wang C, Yuan X, Zhang T, Lin X (2013) Peste des petits ruminants virus exploits cellular autophagy machinery for replication. Virology 437:28–38
The authors declare that they have no competing interests.
XQ and TW performed the majority of experiments. ZL, BY and QX participated part of the experiments. XQ and JW conceived the study, participated in its design and coordination. XQ prepared and revised the manuscript. All authors read and approved the final manuscript.
This research was financially supported by the National Natural Science Foundation of China (Grant Nos. 31572588).
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Additional file 1. Flow chart of the miRNA prediction and differentially expressed (DE) miRNA analysis from goat PBMC infected with PPRV at 1.0 multiplicity of infection (MOI). The 49nt sequence tags from Hiseq sequencing will go through the data cleaning analysis first, then the standard analysis will annotate the clean tags into different categories and take those which cannot be annotated to any category to predict the novel miRNA and seed edit of potential known miRNA. After getting miRNA result, target prediction for miRNA and GO enrichment and KEGG pathway for target genes will be analyzed.
Additional file 2. Summary of deep sequencing data for small RNA (sRNA) in mock- and PPRV-infected goat PBMC. A total of 30 573 869 and 30 644 798 clean reads were obtained from the uninfected and infected groups, respectively. The clean reads were annotated and classified as snRNA, rRNA, snoRNA, Rfam other sncRNA, precursor miRNA, mature miRNA, intergenic, intron, exon, and repeats.
Additional file 3. Details of differentially expressed known and novel microRNA (miRNA) in PPRV- versus mock-infected goat PBMC. A total of 316 miRNA (including 103 known miRNA and 213 novel miRNA) were differentially expressed in mock- and PPRV-infected groups. Among these 316 DEmiRNA, 147 miRNA were upregulated and 169 miRNA were downregulated in the PPRV-infected cells compared to the mock-infected cells.
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Qi, X., Wang, T., Xue, Q. et al. MicroRNA expression profiling of goat peripheral blood mononuclear cells in response to peste des petits ruminants virus infection. Vet Res 49, 62 (2018). https://doi.org/10.1186/s13567-018-0565-3