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- Open Access
A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
© The Author(s) 2018
- Received: 20 November 2017
- Accepted: 3 April 2018
- Published: 2 May 2018
In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J.
Avian leukosis viruses (ALV) can be divided into seven subgroups (A–E, J and K) in the chicken based on the antigenic pattern of the envelope protein. Unlike other subgroups, ALV-J infection mainly induces hematopoietic malignancy with myeloid leukemia and hemangioma [1–3]. Since its first report in 1988 from the UK, ALV-J has spread globally and caused great economic losses in the poultry industry. Due to the lack of a vaccine and anti-viral drugs, the eradication program is the only efficient way to control ALV-J so far . In the ALV-J eradication approach, viral isolation in DF1 cells is the gold standard for ALV-J detection. Traditionally, clinical samples (such as blood, cloaca swab, meconium and sperm) are inoculated into DF1 cells and cultured for 7–9 days before detection by ELISA or IFA, which is time-consuming and less efficient [4–6]. Therefore, shortening the time line for ALV-J isolation is critical for efficient ALV-J eradication. A chicken liver cell line (LMH) was used to explore whether other cell lines could replace DF1 cells for the replication of ALV-J.
In addition to high levels of ALV-J receptors expressed in LMH, the protein expression level for foreign genes in the transfected LMH cell were also investigated. pc-ALV-p27, pc-ALV-J-env and pc-eGFP plasmids were transfected into DF1 and LMH cells respectively and the transfected cells were lysed at the indicated time point. Western blot was performed as described above. As shown in Figures 4C and D, the expression level of ALV-J viral p27 and Env proteins in the transfected LMH cells were much higher than those in the transfected DF1 cells at 12 hpt (hour post-transfection). Notably, the plasmid pcDNA3.1-eGFP in LMH cells also had significantly higher expression levels of eGFP than that in DF1 cells at 12 hpt (Figure 4E). Although the expression level of these proteins in both transfected cells were similar at 24 hpt, only very weak bands were found for DF1 cells at 12 hpt whereas clear and strong bands appear for LMH cells. These data demonstrate that the protein expression system in LMH cells is much more efficient than that in DF1 cells.
It is well known that avian leukosis is one of the vital serious tumor diseases in poultry. In addition to different kinds of tumors, the infection of ALV generally results in immunosuppression in chickens that significantly affects the sustained development of the poultry industry globally . Since no vaccine and anti-viral drug against ALV is now available, the eradication program has been strictly applied in developed countries to control the diseases caused by ALV. The emerging of novel ALV subgroups (such as ALV-J and ALV-K) challenges the current ALV eradication approaches [3, 12–14]. Although many methods have been developed for detection of ALV-J during the stamping-out program, the isolation of ALV-J using DF1 cells is still a well-established standard approach for ALV-J detection. However, the time and low efficacy of the current ALV-J isolation in DF1 cells does not meet well with the requirement for massive detection of ALV-J for its eradication in developing countries, which requires more efficient viral isolation and detection systems for ALV-J. In this study, a chicken liver cell line, LMH, was found to efficiently support the viral replication of ALV-J. Compared to DF1 cells, ALV-J in LMH not only replicated faster, but also yielded higher viral titers. This efficient viral replication of ALV-J in LMH cells can shorten the time of ELISA detection during ALV-J isolation and identification as described in Figure 2. Notably, although DF1 cells were free of the endogenous ALV-E whereas the gp85 mRNA of ALV-E was detected in the LMH cells by RT-PCR (data not shown), both Western blot and ELISA did not detect the endogenous p27 protein in LMH cells. The clinical detection also showed that LMH cells were more sensitive than DF1 cells for detection of ALV. These results indicate that DF1 cells could be replaced by LMH cells for ALV-J detection.
Since the viral receptor in the cells is critical to ALV infection and replication, the finding of the high level of expression of ALV-J receptor chNHE-1 in the LMH cell can explain why ALV-J replicates efficiently and yields higher viral titers in LMH cells when compared with DF1 cells. It should be mentioned that we also detected another two newly identified cell receptors for ALV-J (GRP78 and ANXA2) in both DF1 and LMH cells [10, 15]. However, there was no significant difference for these receptors between the two cell lines (data not shown). The high expression level of chNHE-1 in LMH cells was consistent with the previous study on the distribution of chNHE-1 in the primary chicken liver cell, indicating that the chicken liver cell might be efficient ALV-J target cells . However, whether ALV-J could transform chicken liver cells needs to be further investigated. Because the metabolism of the liver cells are thought to be more active than that of other kinds of cells, we also compared the expression level of foreign genes in both DF1 and LMH cells through transfection studies. Interestingly, we found that both ALV-J related plasmids and ALV-J non-related plasmids could be expressed early in LMH cells with higher levels than that in DF1 cells. The early expression of foreign genes or viral genes in LMH cells with high levels might also contribute to the efficient replication of ALV-J in LMH cells and to the shortening of the time line for ALV-J detection through the good standard method by using LMH cells.
In conclusion, this is the first demonstration that a chicken liver cell line, LMH, with a high level of ALV-J receptors and efficient protein expression system could efficiently support the replication of ALV-J, and could be used as a novel tool to rapidly isolate and identify ALV-J, and in accelerating the ALV-J eradication program and studies on the pathogenesis and oncogenesis of ALV-J. It should also be noted that the characteristics of high-level expression for foreign genes in LMH cells indicates that the LMH cells might also improve viral replication for other viruses and be used to efficiently rescue viruses, if the LMH cell carries the corresponding receptor.
The authors declare that they have no competing interests.
TL and JY conceived and designed the experiments. TL, SS, CX and XD performed the experiments. TL, AQ, HS and JY analysed the data. TL, JX, GL, QX and LL contributed reagents/materials/analysis tools. TL and JY contributed to the writing of the manuscript. TL and JY prepared the figures. All authors read and approved the final manuscript.
We thanks for Dr. Yixin Wang (Shangdong agriculture University, China) for kindly providing us ALV-J infectious clone. This study was supported by the National Key Research & Development (R&D) Plan (2016YFD0501605), the National Natural Science Foundation of China (31472171), Key University Science Research Project of Jiangsu Province (14KJA230002), Special Foundation for State Basic Research Program of China (2013FY113300-4), Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality (26116120), Research Foundation for Talented Scholars in Yangzhou University and the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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- Payne LN, Brown SR, Bumstead N, Howes K, Frazier JA, Thouless ME (1991) A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol 72:801–807View ArticlePubMedGoogle Scholar
- Payne LN, Howes K, Gillespie AM, Smith LM (1992) Host range of Rous sarcoma virus pseudotype RSV(HPRS-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated J. J Gen Virol 73:2995–2997View ArticlePubMedGoogle Scholar
- Payne LN, Nair V (2012) The long view: 40 years of avian leukosis research. Avian Pathol 41:11–19View ArticlePubMedGoogle Scholar
- Maas R, van Zoelen D, Oei H, Claassen I (2006) Replacement of primary chicken embryonic fibroblasts (CEF) by the DF-1 cell line for detection of avian leucosis viruses. Biologicals 34:177–181View ArticlePubMedGoogle Scholar
- Yun B, Li D, Zhu H, Liu W, Qin L, Liu Z, Wu G, Wang Y, Qi X, Gao H, Wang X, Gao Y (2013) Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. J Virol Methods 187:278–283View ArticlePubMedGoogle Scholar
- Qin A, Lee LF, Fadly A, Hunt H, Cui Z (2001) Development and characterization of monoclonal antibodies to subgroup J avian leukosis virus. Avian Dis 45:938–945View ArticlePubMedGoogle Scholar
- Qian K, Liang Y, Yin L, Shao H, Ye J, Qin A (2015) Development and evaluation of an immunochromatographic strip for rapid detection of capsid protein antigen p27 of avian leukosis virus. J Virol Methods 221:115–118View ArticlePubMedGoogle Scholar
- Yin L, Qin A, Qian K, Shao H, Jin W, Shi R, Liang Y, Hang B, Sun W (2013) Development of monoclonal antibodies against p27 of avian leukosis virus and the establishemnt of a sandwich ELISA for detection. China Poult 35:15–19Google Scholar
- Chai N, Bates P (2006) Na+/H+ exchanger type 1 is a receptor for pathogenic subgroup J avian leukosis virus. Proc Natl Acad Sci U S A 103:5531–5536View ArticlePubMedPubMed CentralGoogle Scholar
- Wang L, Mei M, Qin A, Ye J, Qian K, Shao H (2016) Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells. Vet Res 47:92View ArticlePubMedPubMed CentralGoogle Scholar
- Gao Y, Qin L, Pan W, Wang Y, Le Q, Gao H, Wang X (2010) Avian leukosis virus subgroup J in layer chickens, China. Emerg Infect Dis 16:1637–1638View ArticlePubMedPubMed CentralGoogle Scholar
- Cui N, Su S, Chen Z, Zhao X, Cui Z (2014) Genomic sequence analysis and biological characteristics of a rescued clone of avian leukosis virus strain JS11C1, isolated from indigenous chickens. J Gen Virol 95:2512–2522View ArticlePubMedGoogle Scholar
- Li X, Lin W, Chang S, Zhao P, Zhang X, Liu Y, Chen W, Li B, Shu D, Zhang H, Chen F, Xie Q (2016) Isolation, identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China. Arch Virol 161:2717–2725View ArticlePubMedGoogle Scholar
- Shao H, Wang L, Sang J, Li T, Liu Y, Wan Z, Qian K, Qin A, Ye J (2017) Novel avian leukosis viruses from domestic chicken breeds in mainland China. Arch Virol 162:2073–2076View ArticlePubMedGoogle Scholar
- Mei M, Ye J, Qin A, Wang L, Hu X, Qian K, Shao H (2015) Identification of novel viral receptors with cell line expressing viral receptor-binding protein. Sci Rep 5:7935View ArticlePubMedPubMed CentralGoogle Scholar
- Chen B, Pan W, Zhang L, Liu J, Ouyang H, Nie Q, Zhang X (2014) NHE1 gene associated with avian leukosis virus subgroup J infection in chicken. Mol Biol Rep 41:6519–6524View ArticlePubMedGoogle Scholar