Expression level of ALV-J receptor chNHE-1 and protein expression level for foreign genes in DF1 and LMH cells. A Transcription level of chNHE-1 in DF1 and LMH cells were detected with real-time PCR. B Western blot analysis for the expression of chNHE-1 in the DF1 and LMH cells. Lane 1, DF1 cells; lane 2, LMH cells. The gray ratio values of chNHE-1 to GAPDH are shown. C–E Western blot analysis for DF1 cells and LMH cells transfected with 1 μg pc-ALV-p27, pc-ALV-J-env and pc-eGFP respectively, at 12 h and 24 h. Lane 1 and 2, DF1 and LMH cells transfected with the plasmids indicated for 12 h, respectively; Lane 3 and 4, DF1 and LMH cells transfected with the indicated plasmids for 24 h, respectively.