Variability in the response of canine and human dendritic cells stimulated with Brucella canis
- Myriam Pujol1, 2,
- Francisca Castillo2,
- Carla Alvarez2,
- Camila Rojas2,
- Consuelo Borie3,
- Arturo Ferreira†4 and
- Rolando Vernal†2, 5Email author
© The Author(s) 2017
Received: 26 May 2017
Accepted: 16 October 2017
Published: 2 November 2017
Brucella canis is a small intracellular Gram-negative bacterium whose primary host is the dog, but it also can cause mild human brucellosis. One of the main causes of an inefficient immune response against other species of Brucella is their interaction with dendritic cells (DCs), which affects antigen presentation and impairs the development of an effective Th1 immune response. This study analysed the cytokine pattern production, by RT-qPCR and ELISA, in human and canine DCs against whole B. canis or its purified LPS. Human and canine DCs produced different patterns of cytokines after stimulation with B. canis. In particular, while human DCs produced a Th1-pattern of cytokines (IL-1β, IL-12, and TNF-α), canine cells produced both Th1 and Th17-related cytokines (IL-6, IL-12, IL-17, and IFN-γ). Thus, differences in susceptibility and pathogenicity between these two hosts could be explained, at least partly, by the distinct cytokine patterns observed in this study, where we propose that human DCs induce an effective Th1 immune response to control the infection, while canine DCs lead to a less effective immune response, with the activation of Th17-related response ineffective to control the B. canis infection.
Brucella canis, a small Gram-negative facultative intracellular coccobacillus, belonging to the Brucella genus and Proteobacteria phylum, is responsible for canine brucellosis [1, 2]. It is a worldwide distributed zoonotic pathogen whose prevalence is unknown, because routine human brucellosis diagnosis does not include B. canis detection .
Canine brucellosis is considered the main cause of reproductive failures in dogs , provoking late abortions in females and epididymitis and/or prostatitis in male dogs, leading to infertility, and non-reproductive symptoms such as ophthalmitis, diskospondylitis, lymphadenopathy, and splenomegaly [1, 2, 5]. Both, infected dogs and healthy dogs harbouring B. canis have the potential to transmit the bacteria to humans, mainly by oronasal contact, occupational interaction with infected animals, or laboratory accidents [6–9]. When humans are infected, B. canis frequently induce mild or asymptomatic infections that may remain undiagnosed for protracted periods [3, 10–12] and clinical signs include headache, recurrent fever, weakness, fatigue, chills, sweats, and weight loss .
There is strong evidence indicating the relevance of dendritic cells (DCs) function in the pattern of inflammatory-immune response triggered against other Brucella species [14–18]. Due to the differences in the clinical manifestation between dogs and human during B. canis infection, this study aims to assess whether their DCs, when exposed to B. canis, produce different patterns of immune response. We hypothesized that B. canis interacts differently with human and canine DCs leading to distinct pattern of cytokine expression, thus contributing to explain, at least partially, the different susceptibility and pathogenicity observed in dogs and humans.
Materials and methods
Brucella canis isolation and characterization
The B. canis strain SCL was isolated from an urine sample of a male stray dog which had been recently admitted to a dog shelter in Santiago, Chile. The dog had clinical history of urinary symptoms, including inflammation, pain and haematuria, and was positive to a counterimmunoelectrophoresis serological test using B. ovis LPS antigen . Bacterial growth was performed in Brucella agar 5% sheep blood and morphological and biochemical characteristics were compatible with B. canis, including CO2 requirement, oxidase, urease, catalase, citrate utilization, sugar fermentation, and H2S production . The B. canis strain SCL was fully sequenced (Next-Generation Illumina MiSeq Platform®; Illumina Inc., San Diego, CA, USA), at the Favet-Inbiogen Laboratory belonging to the Faculty of Veterinary Sciences, Universidad de Chile, under the GenBank accession number LGAQ00000000.1. Finally, the Brucella genus was confirmed by qPCR, using a Real Time PCR Detection Kit (Genesig®; Southampton, UK) in a StepOnePlus® qPCR equipment (Applied Biosystems, Singapore).
Brucella canis growth conditions and curves
The B. canis strain SCL was cultured on 5% bovine serum tripticase soy agar or 5% horse blood agar (Oxoid Ltd, Hampshire, UK) and incubated at 37 °C under aerobic conditions. Bacterial growth curves were obtained in 5% bovine serum tripticase soy broth (Oxoid Ltd) incubated at 37 °C with constant orbital shaking. Briefly, bacterial samples were inoculated in 4 mL of broth until reaching an optical density (OD) of 0.05, as measured by spectrophotometry at a wavelength of 560 nm (Halo RB-10; Dynamica Scientific Ltd., United Kingdom). The spectrophotometry readings were taken every 1 h and the experiment was stopped when B. canis reached the stationary growth phase (30 h). With each OD measurement, a sample was taken, log10 serially diluted in PBS, and 100 µL of each dilution were cultured on tripticase soy agar and incubated at 37 °C. Finally, the number of colony-forming units/mL (CFU/mL) counted was plotted against the corresponding OD reading. For DC stimulation with whole B. canis, bacteria were taken at the exponential growth phase in order to obtain a reliable number of live bacteria having their whole antigenic potentiality.
Brucella canis LPS purification
For DC stimulation with purified lipopolysaccharide (LPS), B. canis LPS was obtained using a modified version of the TRIzol reagent protocol as described previously . Briefly, B. canis was cultured in 200 mL of tripticase soy broth until it reached the stationary growth phase and was immediately pelleted by centrifugation at 6000 g for 10 min. The pellet was washed with distilled water and then incubated in 3 mL of TRIzol reagent (TRIzol® Plus; Invitrogen Corp., Barcelona, Spain) and 600 µL chloroform (Fluka, Sigma-Aldrich Chemie GmbH, Buchs, Switzerland) at room temperature for 30 min. After centrifugation, the aqueous phase was treated with proteinase K, RNase and DNase (Sigma-Aldrich, St. Louis, MO, USA) and lyophilized overnight. The obtained LPS was then resuspended in 1 mL of 95% ethanol with 0.375 M MgCl2, washed three times with cold 100% ethanol, and purified with 1% Folch reagent containing 2:1 chloroform and methanol. Finally, the LPS was dried overnight in a fume cabinet, quantified using the malondialdehyde thiobarbituric acid reaction, resuspended in milli-Q water as stock solution, and stored at −20 °C . LPS was visualized by sodium dodecyl sulphate-14% polyacrylamide gel electrophoresis (SDS-PAGE) and periodic acid-silver staining as described previously . Coomassie blue stained gels were used to determine absence of protein contamination. B. canis manipulation was carried out inside a biosafety cabinet and approved by the Biosafety Committee from North Campus, Universidad de Chile.
Peripheral blood samples
Peripheral blood samples were obtained from 10 healthy human and 10 healthy dogs. For human samples, buffy coats were obtained from healthy female and male consenting adults from the Clinical Hospital José Joaquín Aguirre, Universidad de Chile. An extensive anamnesis was performed and criteria for individual selection were as follows: absence of fever or manifest infections during the last month, absence of concomitant systemic disease or pregnancy, and no medical history of brucellosis. Further exclusion criteria were positive tests for human immunodeficiency virus, hepatitis B or C viruses, symptomatic allergies, abnormal blood cell counts, increased liver enzymes, or medication of any kind except vitamins and oral contraceptives. The study was approved by the Ethics Committee on Human Research, Universidad de Chile. Dog blood samples were obtained by venipuncture into heparin tubes (Vacutainer®; Becton–Dickinson and Company, New Jersey, NJ, USA) after clinical, hematological and biochemical evaluation, together with counterimmunoelectrophoresis serological analysis, using B. ovis LPS antigen , in order to discard the presence of brucellosis. Dog donors were female and male belonging to private owners, aged 1–7 years, regularly dewormed and vaccinated against distemper, leptospirosis, parvovirus, parainfluenza, canine adenovirus type 2, canine hepatitis, and rabies. Dog owners provided written informed consent approved by the Committee for Animal Care and Use, Universidad de Chile, and all procedures were conducted according to Institutional Ethical Guidelines.
Dendritic cell generation
Human and canine DCs were generated using a three-step protocol as described previously . First, peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation using standard procedures (Ficoll-Paque Plus®; GE Healthcare, Uppsala, Sweden). Second, monocytes were purified from PBMCs by magnetic cell sorting using an anti-CD14 monoclonal antibody conjugated to magnetic beads (MACS®; Miltenyi Biotec, Bergisch Gladbach, Germany). Third, DCs were generated by culturing CD14+ monocytes at 1 × 106 cells/mL for 6 days in RPMI-1640 (Life Technologies, Gaithersburg, MD, USA), supplemented with 10% foetal bovine serum, 100 U/mL penicillin, 100 µg/mL streptomycin, 2 mM l-glutamine (Gibco Invitrogen Corp., Grand Island, NY, USA), 100 U/mL polymixin B (Sigma-Aldrich, St. Louis, MO, USA), and specific recombinant human (rh) or canine (rc) differentiation factors. In particular, canine monocyte cultures were supplemented with 40 ng/mL rhGM-CSF and 30 ng/mL rcIL-4 (R&D Systems Inc., Minneapolis, MN, USA), while human monocyte cultures were supplemented with 20 ng/mL rhGM-CSF and rhIL-4 (R&D Systems Inc.) every 2 days.
Dendritic cell stimulation
Differentiated DCs were then primed with live whole B. canis (MOIs = 2 × 10−1 to 2 × 102) or B. canis purified LPS (10, 100 ng/mL or 1 µg/mL) for 24 h. Dendritic cells stimulated with 1 µg/mL LPS of Escherichia coli strain 0128:B12 (Sigma-Aldrich, St. Louis, MO, USA) and non-stimulated DCs were used as controls. For each subject, the experiments were performed separately. In each experimental step, cell counting was performed in a Neubauer® chamber using a phase contrast microscopy (AxioVert 100; Carl Zeiss Co., Göttingen, Germany) and cell viability was calculated by Trypan blue dye exclusion.
Phenotypic analysis of dendritic cell generation and activation by flow cytometry
The canine and human monocyte differentiation towards DCs and their activation in the presence of B. canis were analyzed by flow cytometry as described previously . Differentiated DCs were stained with anti-CD1a, CD11c, and CD14 monoclonal antibodies (BD Biosciences Pharmingen, San José, CA, USA) for 30 min at 4 °C in the dark and then analyzed using a flow cytometer (BD LSR Fortessa™; Becton–Dickinson, Pleasanton, CA, USA). In order to compare the activation and maturation levels of human DCs upon stimulation with B. canis, the expression levels of CD83 (specific marker of DC maturation) and CD86 (costimulatory signal expressed by DCs and necessary for T-lymphocyte activation during antigen presentation) were determined. In turn, the activation and maturation levels of canine DCs were determined by analysis of the expression of CD86 and DLAII (dog leucocyte antigen class II). Data from 6 independent experiments are expressed as mean ± SD, percentage of CD-positive cells and mean fluorescence intensity (MFI) for each surface marker.
Morphologic cell analysis of stimulated dendritic cells by scanning electron microscopy
The morphological changes observed in B. canis-stimulated human or canine DCs were analyzed by scanning electron microscopy. Briefly, DCs were fixed with 2% glutaraldehyde for 2 h, washed three-times with PBS, dehydrated through immersion in graded ethanol (50, 70, 95 and 100%), and dried at CO2 critical point. Cells were then mounted on aluminium stubs, sputter-coated with gold layer to a thickness of 200 nm (Denton Vacuum Desk V; Moorestown, NJ, USA), and examined in a scanning electron microscope (Jeol JSMIT300LV; Jeol Ltd, Tokyo, Japan) at an accelerating voltage of 20 kV.
Expression of cytokines by RT-qPCR
After whole bacteria or LPS stimulation for 24 h, total cytoplasmic RNA was isolated from DCs using 400 µL of ice-cold lysis buffer containing 0.5% Igepal® CA-630 (Sigma-Aldrich, Saint Louis, MO, USA), 50 mM Tris–HCl pH8, 100 mM NaCl, 5 mM MgCl2, and 10 mM VRC-40 (Gibco Invitrogen, Carlsbad, CA, USA) as described previously . Isolated RNA was quantified using a spectrophotometer (Synergy HT; Bio-Tek Instrument Inc., Winooski, VT, USA) and the first-strand cDNA strand was synthesized using 5 µg of total RNA and a reverse transcription kit following the manufacturer’s protocol (Maxima First Strand cDNA Synthesis kit for RT-qPCR®; Thermo Scientific, Waltham, MA, USA). The mRNA expression for the cytokines IL-1β, IL-4, IL-5, IL-6, IL-10, IL-12p35, IL-13, IL-17, IL-23, IFN-γ, TNF-α, and TGF-β1 were analyzed by RT-qPCR. Briefly, 50 ng of cDNA were amplified using the appropriate canine (Additional file 1) or human primers (Additional file 2) and the KAPA™ SYBR® Fast qPCR reagent (KAPA Biosystems, Woburn, MA, USA) in a StepOnePlus® qPCR equipment (Applied Biosystems) as follows: 95 °C for 3 min, followed by 40 cycles of 95 °C for 3 s and 60 °C for 30 s, and finally a melting curve of 95 °C for 15 s, 60 °C for 1 min and 95 °C for 15 s, for detection of non-specific product formation and false-positive amplification. Calibration curves were performed and a primer matrix was carried out to optimize the concentration of each primer, also in order to confirm the gene specificity for a single amplification product, a Blast search of the selected primers was performed in the Gene Bank sequence database. In human DCs, 18S ribosomal RNA (18S rRNA) expression levels were used as endogenous control. Due to insufficient background regarding the adequate choice of reference gene for cytokine expression in canine DCs, 3 frequently recommended reference genes were analyzed: 18S rRNA, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and TATA box binding protein (TBP) [24–26]. The expression level stability for these reference genes was analyzed using the NormFinder algorithm . Data from 10 independent experiments are shown as mean ± SD. Each experiment was performed in duplicate.
Secretion of cytokines by ELISA
In addition, DC culture supernatants were collected and ELISA analysis was carried out to determine the secretion of human IL-12p70 and TNF-α (Quantikine®; R&D Systems Inc.) and canine IL-17 and IFN-γ (Nori Canine®; Genorise Scientific Inc., Philadelphia, PA, USA). Plates were quantified in an automatic microplate spectrophotometer (Synergy HT; Bio-Tek Instrument Inc.) according the manufacturer’s instructions. Data from 7 independent experiments are shown as mean ± SD.
The flow cytometry data were analyzed using the FlowJo software v9.6.4 (TreeStar, Ashland, ORE, USA), represented as histograms, and expressed as the percentage of positive cells over the total. The RT-qPCR data were analyzed using the StepOne Software v2.2.2 (Applied Biosystems) and the relative quantification was obtained by normalizing the cytokine expression to endogenous control expression using the 2−ΔΔCt method. The ELISA data were calculated using a four-parameter logistic equation. Data were expressed as mean ± SD and statistically analyzed using the GraphPad Prism software v5.0 (GraphPad software Inc., La Jolla, CA, USA). The normality of data distribution was determined using the Kolmogorov–Smirnov test. The differences between groups were determined using the one-way ANOVA test followed by Tukey or Bonferroni post hoc test. Statistical significance was assumed when p < 0.05.
B. canis characterization and growth curves
Brucella canis LPS is compatible with rough LPS
When the whole cellular membrane lysates from B. canis were analyzed (lanes 1–4), silver stained SDS-PAGE (Figure 1F) revealed some upper bands that could be confused with the presence of O-polysaccharide; however, the detection of this bands in Coomassie blue stained gels (Figure 1G) strongly suggest that they correspond to protein structures. When the LPS purified from B. canis was analyzed (lanes 5–8), only bands compatibles with LPS from rough bacteria (core and lipid-A) were detected, and the upper bands were not present in both the silver stained SDS-PAGE gel and the Coomassie blue gel. In general terms, the use of the TRIzol based protocol resulted effective for obtaining a purified LPS from rough B. canis.
Highly pure human and canine dendritic cell cultures were obtained
Stimulation with B. canis induces activation and maturation of canine and human dendritic cells
The 18S rRNA translates a suitable housekeeping gene and allows quantification of cytokine expression by RT-qPCR in canine dendritic cells
Housepeeking genes for quantification of cytokine mRNA expression using RT-qPCR in canine DCs
Canine dendritic cells elicit a Th1/Th17-pattern of cytokine expression while human cells elicit a Th1-pattern of cytokines upon B. canis stimulation
B. canis LPS stimulation induced a Th1/Th17-pattern of cytokine expression in canine DCs and a Th1-pattern of cytokines in human DCs
When stimulated with B. canis, canine and human dendritic cells elicit Th1/Th17 and Th1-pattern of cytokines, respectively
Brucellosis, a disease that affects different animal species, is caused by bacteria of the genus Brucella and some of them have zoonotic potential such as B. melitensis, B. abortus, B. suis, and B. canis . Although these bacteria have the ability to infect humans, B. canis, less infective than the others, is associated with fewer symptomatic diseases and low number of reported cases [1–3]. It has been demonstrated that a large number of B. canis interacts with canine placental tissues and immune cells from different hosts [31–36]; however, there is no evidence of interaction between B. canis and human or canine DCs. Several studies have reported that B. canis has the ability to infect human and murine phagocytes, but failed to demonstrate intracellular proliferation [31, 33, 35]; however, when bacteria are inoculated at the exponential growth phase, they can proliferate in murine macrophages .
In this study, peripheral monocytes from healthy donors were purified and then differentiated into DCs to analyze the effects of whole B. canis or its purified LPS and to compare the production of pro-inflammatory cytokines between canine and human cells. The differentiation and activation of DCs was associated with the loss of the monocyte-macrophage marker CD14 and increase in CD1a, CD11c, CD83, DLAII, and CD86 expression, a pattern consistent with previous studies [23, 38, 39]. In addition, similar levels of DC activation were reached when stimulated with either whole bacteria, demonstrating that B. canis induces canine and human DC activation to a similar extent (Figure 2). Morphological changes consistent with signs of cell activation were also detected by scanning electron microscopy analyses (Figure 3). Thus, the differential DC response triggered shown between dogs and humans may be explained by qualitative rather than quantitative differences in the DC activation, and these differences may be caused by the differential immunogenicity demonstrated by B. canis. The present data indicate that B. canis induced an immune response biased towards both a Th1 and Th17 pattern of cytokine production in canine DCs; however, an immune response biased only towards a Th1 pattern of cytokine production was detected in human DCs (Figures 4, 6). The same cytokine patterns were detected when canine or human DCs were stimulated with B. canis purified LPS, demonstrating that its LPS contributes at least in part to the immune response detected against B. canis (Figures 5, 6).
Dendritic cells are key components of the innate immune response and act as a bridge for the adaptive immune response . They are antigen-presenting cells that circulate through the bloodstream in immature states and are scattered in nearly all tissues. Immature DCs capture microbial antigens and then turn into mature cells with the ability to stimulate naïve T-lymphocytes, determining their Th-polarization and activation pattern, what in turn may define the disease phenotype, in particular whether a mild or severe infection is established . The differential host-dependent pathogenic potential of B. canis observed between dogs and humans could be explained, at least in part, by the differences in the pattern of cytokine production in stimulated DCs detected in the present study.
It has been reported that an effective immune response against Brucella spp. is associated with the Th1-pattern cytokine production by induced DCs and T lymphocytes [15, 18, 42]. In fact, DCs are key target cells for Brucella spp., playing a central role in both the innate recognition and the establishment of a robust adaptive response. Different Brucella spp., however, invade these cells, impairing their capability to eliminate intracellular bacteria and the development of an adequate Th1 immune response .
Although in the present study it was detected an increment in the Th1 promoting cytokines IL-12 and IFN-γ in B. canis-stimulated canine DCs, it was also detected a significant increment in the Th17 polarizing cytokines IL-6 and IL-17, which was an interesting finding due to the lack of previous reports demonstrating the production of IL-17 by canine DCs. In this context, B. canis-stimulated human DCs over-expressed exclusively Th1 polarizing cytokines IL-1β, IL-12, and TNF-α (Figures 4, 6), suggesting that this pattern could lead to an effective Th1 immune response. Production of IL-12 and TNF-α by macrophages and DCs has been directly related with the control B. abortus infection [44, 45] and IL-1β promotes macrophage activation impairing replication of intracellular bacteria such as Mycobacterium tuberculosis by a process mediated by TNF-α secretion and TNFR1 cell surface expression . Interestingly, Th17-type cytokines have been widely described in phagocytes stimulated with different species of Brucella [31, 47, 48]; however, the interpretation of these type of immune response in canine DCs can be complicated, considering that on one hand Th17 is not a fully differentiated phenotype and remain some cell plasticity, which can lead to a protective or harmful immune response in different cases . On the other hand, although the Th17 subset in dogs has been described and associated with chronic inflammation , there is not enough background in this field of canine immunology.
Th17 lymphocytes are considered a potent pro-inflammatory and osteoclastogenic cell population, closely associated with autoimmune disorders . In addition, although the Th17-related immune response can be effective against extracellular bacterial or fungal infections, it is considered ineffective to control intracellular bacterial infections [51, 52]. The Th17 cell subset has been associated with the development of osteoarticular lesions in different hosts infected by Brucella spp. [31, 48, 53], although it has been also suggested that the combination Th1/Th17 response induced by oral vaccines would be suitable to control Brucella spp. infection [47, 54]. There is evidence indicating that only a Th1 response is protective against B. melitensis and the development of a Th17 combined immune response do not affect the course of infection . One possible explanation for these opposite effects is that the Th17 protective role refers to a local immune response in the gut, where Th17 lymphocytes usually play an important defensive role maintaining the mucosal homeostasis and integrity [52, 56]. In fact, there is evidence that an oral vaccine can lead to a protective Th1/Th17 response that protects mice from an oral administration of live Brucella, but the parenteral administration of the same antigen lead to a Th1 response, that protects mice from a systemic challenge .
Mycobacterium tuberculosis is a widely studied intracellular bacterium that induces a protective Th1 response, with the parallel development of a detrimental Th17 response that contributes with the clinical symptoms and favours the formation of granulomas in affected organs . It is well known that IL-17 promotes granulopoiesis , although some Brucella spp. are differently resistant to neutrophil response ; furthermore, different Brucella spp., including B. canis, are able to resist killing by bovine neutrophils . Considering that the main consequences of B. canis infection are osteoarticular lesions such as diskospondylitis and the presence of granulomatous lesions in affected organs [1, 60, 61], it is possible to assume that the presence of IL-6 and IL-17 could lead to a Th17-pattern of immune response, contributing to the brucellosis pathogenicity in infected dogs. In fact, increased serological levels of IL-17 are detected in human patients affected of acute brucellosis and these levels decrease after receiving antibiotic treatment, which indicates this cytokine is directly involved with the pathogenesis of human brucellosis .
In human Th lymphocytes, the Th17 cell plasticity can lead to different subsets, including IL-17/IL-10 secreting cells that in some cases can be protective for some bacterial infections; however, Th17 cells can also acquire the ability of IL-17/IFN-γ production that promote chronicity in inflammatory disorders [56, 63]. When Th17 knockout mice were infected with B. melitensis, no effect was described after elimination of this cell subset, revealing that Th17 cells may not affect the course of the infection . In relation to this, although Th17 lymphocytes have been implicated with pathogenicity of rheumatoid arthritis, many IL-17 targeted therapies have failed to control the disease signs and symptoms . However, the use of antibodies that neutralize TNF-α, a cytokine that can bias Th17 cells towards pathogenic Th1/Th17 cells, can specifically decrease this cell subset, improving symptomatic lesions [56, 63]. This indicate that a variety of Th17 cells can be induced and differentially affect the immune response.
The results of the present investigation strongly suggest that the differences in the cytokine patterns observed between canine and human DCs could have an impact in the development of the B. canis infection. Thus, a more effective Th1 immune response is displayed in human, while a Th1/Th17 immune response is displayed in dogs, impairing the elimination of the bacteria and favoring its pathogenicity. The involvement of the Th17-type immune response could also explain the long periods of asymptomatic relapses, because these cells are characterized by long periods of dormant phases, followed by short periods of cell activation. Taken together, these data contribute at least in part to elucidate the canine brucellosis immunopathology and to a better understanding of canine immunology through the description of IL-17 producing canine DCs.
The authors declare that they have no competing interests.
MP did the scientific work with the support of AF and RV, designed and implemented the study, processed the biological samples, performed the cell cultures, carried out the ELISA experiments, performed the data analysis, and critically evaluated and supplemented the manuscript. CR and CB carried out the microbiology experiments. MP, FC and CA carried out the RT-qPCR experiments. MP, AF and RV prepared the figures and the manuscript for submission. All authors read and approved the final manuscript.
The authors thank Jocelyn Arias and Leonor Diaz (Microbiology Laboratory) for their generous support with bacterial cultures and LPS purification, Daniela del Pino for her support in blood sample procedures of canine donors, Rocío Orellana (Scanning electron microscopy Laboratory), Barbara Pesce (FACS-Laboratory) and Milton Larrondo and Claudio Pérez from the Clinical Hospital of Universidad de Chile.
This work was financially supported by the Comisión Nacional de Investigación Científica y Tecnológica (CONICYT) from the Chilean Government, Grant FONDECYT 1140904. MP was a recipient of the fellowship CONICYT 21130626.
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- Hollett RB (2006) Canine brucellosis: outbreaks and compliance. Theriogenology 66:575–587View ArticlePubMedGoogle Scholar
- Wanke MM (2004) Canine brucellosis. Anim Reprod Sci 82–83:195–207View ArticlePubMedGoogle Scholar
- Lucero NE, Corazza R, Almuzara MN, Reynes E, Escobar GI, Boeri E, Ayala SM (2010) Human Brucella canis outbreak linked to infection in dogs. Epidemiol Infect 138:280–285View ArticlePubMedGoogle Scholar
- Keid LB, Chiebao DP, Batinga MCA, Faita T, Diniz JA, Oliveira T, Ferreira HL, Soares RM (2017) Brucella canis infection in dogs from commercial breeding kennels in Brazil. Transbound Emerg Dis 64:691–697View ArticlePubMedGoogle Scholar
- Buzgan T, Karahocagil MK, Irmak H, Baran AI, Karsen H, Evirgen O, Akdeniz H (2010) Clinical manifestations and complications in 1028 cases of brucellosis: a retrospective evaluation and review of the literature. Int J Infect Dis 14:e469–e478View ArticlePubMedGoogle Scholar
- Young EJ (1995) An overview of human brucellosis. Clin Infect Dis 21:283–289View ArticlePubMedGoogle Scholar
- Wallach JC, Giambartolomei GH, Baldi PC, Fossati CA (2004) Human infection with M-strain of Brucella canis. Emerg Infect Dis 10:146–148View ArticlePubMedPubMed CentralGoogle Scholar
- Marzetti S, Carranza C, Roncallo M, Escobar GI, Lucero NE (2013) Recent trends in human Brucella canis infection. Comp Immunol Microbiol Infect Dis 36:55–61View ArticlePubMedGoogle Scholar
- Angel MO, Ristow P, Ko AI, Di-Lorenzo C (2012) Serological trail of Brucella infection in an urban slum population in Brazil. J Infect Dev Ctries 6:675–679View ArticlePubMedPubMed CentralGoogle Scholar
- Lucero NE, Escobar GI, Ayala SM, Jacob N (2005) Diagnosis of human brucellosis caused by Brucella canis. J Med Microbiol 54:457–461View ArticlePubMedGoogle Scholar
- Lucero NE, Jacob NO, Ayala SM, Escobar GI, Tuccillo P, Jacques I (2005) Unusual clinical presentation of brucellosis caused by Brucella canis. J Med Microbiol 54:505–508View ArticlePubMedGoogle Scholar
- Lucero NE, Maldonado PI, Kaufman S, Escobar GI, Boeri E, Jacob NR (2010) Brucella canis causing infection in an HIV-infected patient. Vector Borne Zoonotic Dis 10:527–529View ArticlePubMedGoogle Scholar
- Sayan M, Erdenlig S, Stack J, Kilic S, Guducuoglu H, Aksoy Y, Baklan A, Etiler N (2011) A serological diagnostic survey for Brucella canis infection in Turkish patients with Brucellosis-like symptoms. Jpn J Infect Dis 64:516–519PubMedGoogle Scholar
- Archambaud C, Salcedo SP, Lelouard H, Devilard E, de Bovis B, Van Rooijen N, Gorvel JP, Malissen B (2010) Contrasting roles of macrophages and dendritic cells in controlling initial pulmonary Brucella infection. Eur J Immunol 40:3458–3471View ArticlePubMedGoogle Scholar
- Salcedo SP, Marchesini MI, Lelouard H, Fugier E, Jolly G, Balor S, Muller A, Lapaque N, Demaria O, Alexopoulou L, Comerci DJ, Ugalde RA, Pierre P, Gorvel JP (2008) Brucella control of dendritic cell maturation is dependent on the TIR-containing protein Btp1. PLoS Pathog 4:e21View ArticlePubMedPubMed CentralGoogle Scholar
- Billard E, Cazevieille C, Dornand J, Gross A (2005) High susceptibility of human dendritic cells to invasion by the intracellular pathogens Brucella suis, B. abortus, and B. melitensis. Infect Immun 73:8418–8424View ArticlePubMedPubMed CentralGoogle Scholar
- Atluri VL, Xavier MN, de Jong MF, den Hartigh AB, Tsolis RE (2011) Interactions of the human pathogenic Brucella species with their hosts. Annu Rev Microbiol 65:523–541View ArticlePubMedGoogle Scholar
- Ni M, Martire D, Scotet E, Bonneville M, Sanchez F, Lafont V (2012) Full restoration of Brucella-infected dendritic cell functionality through Vγ9Vδ2 T helper type 1 crosstalk. PLoS One 7:e43613View ArticlePubMedPubMed CentralGoogle Scholar
- Borie C, Cepeda R, Villarroel M, De Los Reyes M (2002) Description of reproductive characteristics of three Brucella canis seropositive dogs. Arch Med Vet 34:111–116View ArticleGoogle Scholar
- Alton GG, Jones LM, Pietz DE (1975) Laboratory techniques in brucellosis. Monogr Ser World Health Organ. 55:1–163Google Scholar
- Yi EC, Hackett M (2000) Rapid isolation method for lipopolysaccharide and lipid A from Gram-negative bacteria. Analyst 125:651–656View ArticlePubMedGoogle Scholar
- Tsai CM, Frasch CE (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119:115–119View ArticlePubMedGoogle Scholar
- Vernal R, León R, Herrera D, Garcia-Sanz JA, Silva A, Sanz M (2008) Variability in the response of human dendritic cells stimulated with Porphyromonas gingivalis or Aggregatibacter actinomycetemcomitans. J Periodontal Res 43:689–697View ArticlePubMedGoogle Scholar
- Ayers D, Clements DN, Salway F, Day PJ (2007) Expression stability of commonly used reference genes in canine articular connective tissues. BMC Vet Res 3:7View ArticlePubMedPubMed CentralGoogle Scholar
- Chamizo C, Rubio JM, Moreno J, Alvar J (2001) Semi-quantitative analysis of multiple cytokines in canine peripheral blood mononuclear cells by a single tube RT-PCR. Vet Immunol Immunopathol 83:191–202View ArticlePubMedGoogle Scholar
- Chimura N, Shibata S, Kimura T, Kondo N, Mori T, Hoshino Y, Kamishina H, Maeda S (2011) Suitable reference genes for quantitative real-time RT-PCR in total RNA extracted from canine whole blood using the PAXgene™ system. J Vet Med Sci 73:1101–1104View ArticlePubMedGoogle Scholar
- Andersen CL, Jensen JL, Ørntoft TF (2004) Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res 64:5245–5250View ArticlePubMedGoogle Scholar
- Fernandes AR, de Azevedo SS, Pinheiro ES, Genovez ME, de Azevedo AS, de Sousa Américo Batista C, Alves CJ (2011) Brucella canis infection in dogs attended in veterinary clinics from patos, Paraíba state, Brazil. Braz J Microbiol 42:1405–1408View ArticlePubMedPubMed CentralGoogle Scholar
- Flores-Castro R, Suarez F, Ramirez-Pfeiffer C, Carmichael LE (1977) Canine brucellosis: bacteriological and serological investigation of naturally infected dogs in Mexico City. J Clin Microbiol 6:591–597PubMedPubMed CentralGoogle Scholar
- Haque N, Bari MS, Hossain MA, Muhammad N, Ahmed S, Rahman A, Hoque SM, Islam A (2011) An overview of Brucellosis. Mymensingh Med J 20:742–747PubMedGoogle Scholar
- Delpino MV, Fossati CA, Baldi PC (2009) Proinflammatory response of human osteoblastic cell lines and osteoblast-monocyte interaction upon infection with Brucella spp. Infect Immun 77:984–995View ArticlePubMedGoogle Scholar
- Detilleux PG, Deyoe BL, Cheville NF (1990) Entry and intracellular localization of Brucella spp. in vero cells: fluorescence and electron microscopy. Vet Pathol 27:317–328View ArticlePubMedGoogle Scholar
- Eskra L, Covert J, Glasner J, Splitter G (2012) Differential expression of iron acquisition genes by Brucella melitensis and Brucella canis during macrophage infection. PLoS One 7:e31747View ArticlePubMedPubMed CentralGoogle Scholar
- Martin-Martin AI, Vizcaino N, Fernandez-Lago L (2010) Cholesterol, ganglioside GM1 and class A scavenger receptor contribute to infection by Brucella ovis and Brucella canis in murine macrophages. Microbes Infect 12:246–251View ArticlePubMedGoogle Scholar
- Rittig MG, Kaufmann A, Robins A, Shaw B, Sprenger H, Gemsa D, Foulongne V, Rouot B, Dornand J (2003) Smooth and rough lipopolysaccharide phenotypes of Brucella induce different intracellular trafficking and cytokine/chemokine release in human monocytes. J Leukoc Biol 74:1045–1055View ArticlePubMedGoogle Scholar
- Gyuranecz M, Szeredi L, Ronai Z, Denes B, Dencso L, Dan A, Palmai N, Hauser Z, Lami E, Makrai L, Erdelyi K, Janosi S (2011) Detection of Brucella canis-induced reproductive diseases in a kennel. J Vet Diagn Invest 23:143–147View ArticlePubMedGoogle Scholar
- Chacon-Diaz C, Altamirano-Silva P, Gonzalez-Espinoza G, Medina MC, Alfaro-Alarcon A, Bouza-Mora L, Jimenez-Rojas C, Wong M, Barquero-Calvo E, Rojas N, Guzman-Verri C, Moreno E, Chaves-Olarte E (2015) Brucella canis is an intracellular pathogen that induces a lower proinflammatory response than smooth zoonotic counterparts. Infect Immun 83:4861–4870View ArticlePubMedPubMed CentralGoogle Scholar
- Díaz-Zúñiga J, Yáñez JP, Alvarez C, Melgar-Rodríguez S, Hernández M, Sanz M, Vernal R (2014) Serotype-dependent response of human dendritic cells stimulated with Aggregatibacter actinomycetemcomitans. J Clin Periodontol 41:242–251View ArticlePubMedGoogle Scholar
- Vernal R, León R, Silva A, van Winkelhoff AJ, Garcia-Sanz JA, Sanz M (2009) Differential cytokine expression by human dendritic cells in response to different Porphyromonas gingivalis capsular serotypes. J Clin Periodontol 36:823–829View ArticlePubMedGoogle Scholar
- Banchereau J, Briere F, Caux C, Davoust J, Lebecque S, Liu YJ, Pulendran B, Palucka K (2000) Immunobiology of dendritic cells. Annu Rev Immunol 18:767–811View ArticlePubMedGoogle Scholar
- Smyth CM, Logan G, Boadle R, Rowe PB, Smythe JA, Alexander IE (2005) Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy. Int Immunol 17:123–132View ArticlePubMedGoogle Scholar
- Billard E, Dornand J, Gross A (2007) Brucella suis prevents human dendritic cell maturation and antigen presentation through regulation of tumor necrosis factor alpha secretion. Infect Immun 75:4980–4989View ArticlePubMedPubMed CentralGoogle Scholar
- Skendros P, Boura P (2013) Immunity to brucellosis. Rev Sci Tech 32:137–147View ArticlePubMedGoogle Scholar
- Oliveira FS, Carvalho NB, Brandao AP, Gomes MT, de Almeida LA, Oliveira SC (2011) Interleukin-1 receptor-associated kinase 4 is essential for initial host control of Brucella abortus infection. Infect Immun 79:4688–4695View ArticlePubMedPubMed CentralGoogle Scholar
- Sathiyaseelan J, Goenka R, Parent M, Benson RM, Murphy EA, Fernandes DM, Foulkes AS, Baldwin CL (2006) Treatment of Brucella-susceptible mice with IL-12 increases primary and secondary immunity. Cell Immunol 243:1–9View ArticlePubMedGoogle Scholar
- Jayaraman P, Sada-Ovalle I, Nishimura T, Anderson AC, Kuchroo VK, Remold HG, Behar SM (2013) IL-1β promotes antimicrobial immunity in macrophages by regulating TNFR signaling and caspase-3 activation. J Immunol 190:4196–4204View ArticlePubMedPubMed CentralGoogle Scholar
- Clapp B, Skyberg JA, Yang X, Thornburg T, Walters N, Pascual DW (2011) Protective live oral brucellosis vaccines stimulate Th1 and th17 cell responses. Infect Immun 79:4165–4174View ArticlePubMedPubMed CentralGoogle Scholar
- Giambartolomei GH, Scian R, Acosta-Rodriguez E, Fossati CA, Delpino MV (2012) Brucella abortus-infected macrophages modulate T lymphocytes to promote osteoclastogenesis via IL-17. Am J Pathol 181:887–896View ArticlePubMedGoogle Scholar
- Basu R, Hatton RD, Weaver CT (2013) The Th17 family: flexibility follows function. Immunol Rev 252:89–103View ArticlePubMedPubMed CentralGoogle Scholar
- Kol A, Walker NJ, Nordstrom M, Borjesson DL (2016) Th17 pathway as a target for multipotent stromal cell therapy in dogs: implications for translational research. PLoS One 11:e0148568View ArticlePubMedPubMed CentralGoogle Scholar
- Audic S, Lescot M, Claverie JM, Scholz HC (2009) Brucella microti: the genome sequence of an emerging pathogen. BMC Genomics 10:352View ArticlePubMedPubMed CentralGoogle Scholar
- Muranski P, Restifo NP (2013) Essentials of Th17 cell commitment and plasticity. Blood 121:2402–2414View ArticlePubMedPubMed CentralGoogle Scholar
- Giambartolomei GH, Arriola Benitez PC, Delpino MV (2017) Brucella and osteoarticular cell activation: partners in crime. Front Microbiol 8:256View ArticlePubMedPubMed CentralGoogle Scholar
- Pasquevich KA, Ibanez AE, Coria LM, Garcia Samartino C, Estein SM, Zwerdling A, Barrionuevo P, Oliveira FS, Seither C, Warzecha H, Oliveira SC, Giambartolomei GH, Cassataro J (2011) An oral vaccine based on U-Omp19 induces protection against B. abortus mucosal challenge by inducing an adaptive IL-17 immune response in mice. PLoS One 6:e16203View ArticlePubMedPubMed CentralGoogle Scholar
- Vitry MA, De Trez C, Goriely S, Dumoutier L, Akira S, Ryffel B, Carlier Y, Letesson JJ, Muraille E (2012) Crucial role of IFN-γ-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. Infect Immun 80:4271–4280View ArticlePubMedPubMed CentralGoogle Scholar
- Bystrom J, Taher TE, Muhyaddin MS, Clanchy FI, Mangat P, Jawad AS, Williams RO, Mageed RA (2015) Harnessing the therapeutic potential of Th17 cells. Mediators Inflamm 2015:205156View ArticlePubMedPubMed CentralGoogle Scholar
- Lyadova IV, Panteleev AV (2015) Th1 and Th17 cells in tuberculosis: protection, pathology, and biomarkers. Mediators Inflamm 2015:854507View ArticlePubMedPubMed CentralGoogle Scholar
- Young EJ, Borchert M, Kretzer FL, Musher DM (1985) Phagocytosis and killing of Brucella by human polymorphonuclear leukocytes. J Infect Dis 151:682–690View ArticlePubMedGoogle Scholar
- Keleher LL, Skyberg JA (2016) Activation of bovine neutrophils by Brucella spp. Vet Immunol Immunopathol 177:1–6View ArticlePubMedGoogle Scholar
- Anderson GI, Binnington AG (1983) Discospondylitis and orchitis associated with high Brucella titre in a dog. Can Vet J 24:249–252PubMedPubMed CentralGoogle Scholar
- Kerwin SC, Lewis DD, Hribernik TN, Partington B, Hosgood G, Eilts BE (1992) Diskospondylitis associated with Brucella canis infection in dogs: 14 cases (1980–1991). J Am Vet Med Assoc 201:1253–1257PubMedGoogle Scholar
- Sofian M, Ramezani A, Mousavi A, Banifazl M, Cherei S, Cherei A, Aghakhani A (2016) Interleukin-17 and TGF-β levels in patients with acute brucellosis before and after treatment. Turk J Med Sci 46:1348–1352View ArticlePubMedGoogle Scholar
- Annunziato F, Cosmi L, Liotta F, Maggi E, Romagnani S (2014) Human Th1 dichotomy: origin, phenotype and biologic activities. Immunology. doi:10.1111/imm.12399 PubMedGoogle Scholar
- Alunno A, Manetti M, Caterbi S, Ibba-Manneschi L, Bistoni O, Bartoloni E, Valentini V, Terenzi R, Gerli R (2015) Altered immunoregulation in rheumatoid arthritis: the role of regulatory T cells and proinflammatory Th17 cells and therapeutic implications. Mediators Inflamm 2015:751793PubMedPubMed CentralGoogle Scholar