- Research article
- Open Access
What variables are important in predicting bovine viral diarrhea virus? A random forest approach
© Machado et al. 2015
Received: 12 February 2015
Accepted: 6 July 2015
Published: 24 July 2015
Bovine viral diarrhea virus (BVDV) causes one of the most economically important diseases in cattle, and the virus is found worldwide. A better understanding of the disease associated factors is a crucial step towards the definition of strategies for control and eradication. In this study we trained a random forest (RF) prediction model and performed variable importance analysis to identify factors associated with BVDV occurrence. In addition, we assessed the influence of features selection on RF performance and evaluated its predictive power relative to other popular classifiers and to logistic regression. We found that RF classification model resulted in an average error rate of 32.03% for the negative class (negative for BVDV) and 36.78% for the positive class (positive for BVDV).The RF model presented area under the ROC curve equal to 0.702. Variable importance analysis revealed that important predictors of BVDV occurrence were: a) who inseminates the animals, b) number of neighboring farms that have cattle and c) rectal palpation performed routinely. Our results suggest that the use of machine learning algorithms, especially RF, is a promising methodology for the analysis of cross-sectional studies, presenting a satisfactory predictive power and the ability to identify predictors that represent potential risk factors for BVDV investigation. We examined classical predictors and found some new and hard to control practices that may lead to the spread of this disease within and among farms, mainly regarding poor or neglected reproduction management, which should be considered for disease control and eradication.
Bovine viral diarrhea virus (BVDV) has a single-stranded, positive-sense RNA genome and belongs to the genus Pestivirus of the family Flaviviridae , causing one of the most common and economically important viral diseases of cattle . Several BVDV control strategies have been proposed and launched in many countries based on information about prevalence, incidence and associated risk factors, which is the baseline knowledge required for designing and implementing effective regional control actions .
A number of studies based on traditional risk factors identification approaches (logistic regression mainly) have been performed on BVDV [4-8], and the knowledge about major risk factors are related to the following: biosecurity , reproduction management [2,6,9,10], herd size [5,8], animal introduction [2,4,5,11], direct contact with other animals (from the same species or not) [4,11-13], communal grazing [4,5], age of animals [5,14], artificial insemination (AI) , and natural mating . Nonetheless, usual epidemiologic analytic frameworks like logistic regression are often limited for the analysis of high-dimensional, imbalanced and nonlinear data, and may be poorly adapted to epidemiological datasets with a large number of predictor variables (parameters) in relation to the number of observations given the high susceptibility to overfitting [16,17].
Feature selection methods provided by machine learning (ML) approaches are an interesting, flexible and robust alternative for identifying predictors that contribute to disease occurrence. Among these, the random forest (RF) algorithm  has been regarded as one of the most precise prediction methods, having advantages such as ability to determine variable importance, ability to model complex interactions among independent variables, and flexibility to perform several types of statistical data analysis, including regression, classification and unsupervised learning . Briefly, RF builds a collection of decision trees based on randomly and independently selected subsets of data, and a simple majority vote among all trees in the forest is taken for class prediction. A clear difference from traditional statistical frameworks is that RF performs a data-driven analysis without making a priori assumptions about the structure of data or the relationship between the response and independent variables, and is less sensitive to spatial autocorrelation and multicollinearity issues [17,20]. Its high predictive power has been supported by previous comparative studies with other ML methods [21-25].
The use of RF allows for a new way of modeling and extracting information from observational data, thus contributing to a better understanding of a target system and mechanism that are, in general, complex and nonlinear. However, according to the authors’ knowledge, there are a limited number of studies in veterinary epidemiology that adopt ML-based methods, and most of them still neglect the importance of proper and careful tuning of models parameters [26-28]. For example, RF was used in a cross-sectional study that aimed at assessing risk factors that may have led to spillover of pH1N1 from humans to swine in Cameroon, Central Africa . In human epidemiology, RF has been already applied in Diabetic Retinopathy (DR) classification analysis for early detection of this illness based on clinical and fundus photography data . Results suggested that RF was a valuable tool to diagnose DR, producing higher classification accuracy than logistic regression, and that the most relevant variables detected by this ML algorithm are meaningful and correlate well with known risk factors.
In this paper, we aim to investigate the use of RF in the analysis of cross-sectional data collected in a BVDV prevalence study. As previously discussed, the application of this ML algorithm is still uncommon for this type of task. Hence, this study has the following main objectives: (1) train a RF model that provides a good predictive power for the collected data, (2) perform a variable importance analysis using the RF model and the well-established Gini index method to identify potential BVDV predictors, (3) investigate the effect of feature selection on the overall performance of the RF model, carefully assessing the impact on the accuracy and the sensitivity-specificity balance, and, finally, (4) compare RF performance with that obtained by other popular ML algorithms and by logistic regression, examining their predictive power and robustness on the scenario of interest.
Materials and methods
Based on data collected from a prevalence study of reproductive disease in dairy cattle in the State of Rio Grande do Sul, Brazil, a RF model was trained and evaluated with respect to model accuracy, followed by variable importance analysis. All procedures performed for this study was approved by the Institutional Animal Care and Use Committee (Federal University of Rio Grande do Sul, project number: 28288, Porto Alegre, Brazil).
Study design-data collection
Study area and target population
Rio Grande do Sul is the southernmost state of Brazil, with a total area of 268 781.896 km2 and 497 municipalities. The cattle population is approximately 13.5 million, 10% of which are dairy cattle . Rio Grande do Sul is the second largest milk-producing state, in which milk production is clustered in six well-defined regions . The study area is explained in more detail in .
The target population of data collection included all dairy herds in the state of Rio Grande do Sul. According to the official data from the Office of Agriculture, Livestock and Agribusiness of the State of Rio Grande do Sul 81 307 dairy herds were registered. Descriptive statistics of the studied population can be found in Additional file 1.
Survey design and sample collection
First, a cross-sectional survey was performed to estimate the BVDV, Neospora caninum and Infectious Bovine Rhinotracheitis (IBR) prevalence in dairy herds based on (bulk tank milk) BTM samples and to identify the associated risk factors, required by the Office of Agriculture, Livestock and Agribusiness of the State of Rio Grande do Sul. A one-stage stratified random sample design was used. Those farms from which one BTM sample was collected were considered a sampling unit. A stratified sample, which was proportional to the herd population present in each of the seven regions, was performed, and each herd was randomly sampled from all the individual strata. These regions are subdivisions of Brazilian states that are grouped according to proximity and share common agroecological characteristics. The sample size was calculated using R Foundation for Statistical Computing, Vienna, Austria (Package EpiCalc), considering the following parameters: total dairy herds registered at the moment (81 307), 50% expected prevalence, 95% confidence interval, and 5% of absolute precision. The minimum sample size required was 384 dairy herds; however, 388 herds were collected to have a safety margin of extra farm samples.
Bulk tank milk collection
For each herd, a total of 12 mL of milk was collected directly from the milk container immediately after the entire volume had been homogenized. During sampling and transportation, the raw milk was kept under refrigeration between 2 and 8 °C without preservatives. Following an overnight rest, a 1.2 mL sample of skim milk was collected and kept at −20 °C until analysis.
Serological assay and interpretation
Herds with PI ≥ 30% were considered to have a high probability to harboring an active infection and/or to have at least one positive cow contributing to the sample.
Each decision tree composing the forest has the standard flowchart-like structure, in which internal (split) nodes test variables and branch out according to their possible values, and leaf (terminal) nodes assign a classification for all instances that reach the leaf. The tree growing process in RF is also based in binary recursive splitting that aims at maximizing the decrease of impurity at each node, where impurity can be evaluated by heterogeneity for classification trees (if the response is of categorical type). Nonetheless, in constructing the ensemble of trees, RF incorporates two types of randomness. First, each tree is built using a random bootstrap sample of the original training data (~2/3 of samples), drawn by sampling with replacement (Figure 2A). Second, at each candidate split in the tree growing process, a subset of variables is randomly selected among all available variables to decide node splitting, and the best split among these variables is chosen based on the smallest node impurity [18,33]. Here, we adopt the well-known Gini index as a measure of node impurity. The tree growing procedure is performed recursively until a minimum node size is reached, which is parameterized by the user, or until no further improvement can be made . The two main parameters of the RF algorithms are the number of random variables (predictors) to evaluate at each node split and the number of trees to grow in the ensemble.
The methodology underlying the RF algorithm has interesting properties that make it especially appealing for classification tasks. To begin with, the mechanism applied for tree growing allows the estimation of the most important variables for classification, and generates an internal unbiased estimate of the generalized error drawn from the data left out of the bootstrap sample used as a training set, called out-of-bag (OOB) data, which corresponds to about ~1/3 of the original data. In addition, the fact that the predicted class represents the mode of the outputs returned by individual trees gives robustness to this ensemble classifier in relation to a single tree. Finally, the bootstrapping procedure and the out-of-bag estimates make RF more accurate and less sensitive to issues such as overfitting, outliers and confounding in comparison to other statistical and machine learning methods [18,33].
Given the severe class imbalance observed in the data and the general difficulty of machine learning methods to handle this issue , we have incorporated a down-sampling procedure in the model learning functions provided by the caret R package, which samples the majority class to make its frequency closer to the rarest class. This procedure aims at avoiding the ML algorithm’s tendency to be strongly biased towards the majority class, consequently misclassifying a lot of instances related to the minority class.
The original dataset was randomly and uniformly (i.e., maintaining the same proportion of classes as in the original dataset) split into a training set (80% of observations) and an independent testing set (20% of observations). This subdivision reflects an attempt to compose a minimum sample size that would be representative in future applications of the model and is a common strategy for evaluating ML models when external validation data is not available. The training set was applied for training our classifier using a cross-validation process and the testing set was further used to compare models performance based on independent test data.
The set of 40-predictor variables collected in the survey performed and used to train the BVDV classification model were: (1) who inseminates the animals, (2) number of neighboring farms that have cattle, (3) what proportion of the farm income is based on milk production, (4) for how many years has the farm produced milk, (5) frequency of technical assistance, (6) is rectal palpation performed routinely, (7) the number of different inseminators in the last year, (8) what is the origin of the bulls, (9) frequency of veterinary assistance, (10) are the animals placed in quarantine before introduction, (11) what is the origin of animals brought into the farm, (12) how often does the fence between/among farms that hold cattle collapse, (13) how the cows are milked, (14) was there an increase in abortions, (15) does calving occur in closed barns, (16) number of cows lactating at the sampling moment, (17) were animals vaccinated for BVDV, (18) was there a rise of mating failure, (19) do animals share the same feed and water containers, (20) number of cows not lactating at the sampling moment, (21) is colostrum stock available, (22) total farm area in hectares, (23) are paddocks available for sick animals, (24) who administers the medications, (25) is blood from a sick animal injected into the healthy ones (“Premunição”), (26) within the last year have animals been sent to fairs, (27) has the farmer seen weak born calves, (28) were pregnant cows introduced, (29) total area for cow farming, (30) has the farmer seen weak calves, (31) were new animals introduced in the last year, (32) possibility of direct contact (over the fence) between animals from the neighboring farm, (33) animals are grouped based in age category, (34) is the inseminator always the same, (35) does the farm have technical assistance, (36) is natural mating used, (37) does the farm have bulk milk tank, (38) is artificial insemination used, (39) does calving occurs in the fields, (40) does the farm have veterinary assistance. See Additional file 2 for the frequency of important predictor variables.
The RF model was trained with the training set derived from the original data (i.e., 80% of data) and the complete set of variables using the randomForest package in R. The number of trees induced in the training process was configured to 500 trees following the suggestion of the authors, and the number of variables (mtry) randomly sampled as candidates for node splitting during the tree growing process was optimized using the caret package in the R environment. In training the model, we adopted a repeated 10-fold cross-validation technique to better estimate its performance and generalization power, and to prevent overfitting and artificial accuracy improvement due to use of the same data for training and testing the classifier.
Once the model was trained, we investigated the effect of multicollinearity over the performance of RF. For this purpose, we computed the correlation matrix for the set of 40 variables using Pearson correlation and identified highly correlated predictors among our independent variables. Next, we selected some of the highly correlated variables to discard from the analysis based on plausibility criteria and repeated the RF training process without these variables, comparing its performance with the original RF model.
An interesting property of RF is that it naturally provides estimates of variable importance, which are computed during model training by evaluating the average decrease in the nodes’ impurity measured by Gini index. The importance of a variable is defined as the Gini index reduction for the variable summed over all nodes for each tree in the forest, normalized by the number of trees . Hence, the higher the Gini importance, the more relevant that variable is for maintaining the predictive power of the RF model. Although RF are capable of modeling a large number of variables and achieving good prediction performance, finding a small number of variables with equivalent or better prediction ability is highly desired not only because it is helpful for interpretation, but also easy for practical use as strategies for disease control .
Thus, after running the first round of model training and obtaining the Gini importance for each of the 40-predictor variables of our data set, we performed a restricted forward feature selection and verified the impact of variables inclusion over the model’s predictive accuracy in an incremental fashion. This step aims at identifying irrelevant variables that may mislead the algorithm and increase the generalization error . Specifically, we trained several RF models, starting from a model trained upon a single variable, and subsequently adding new variable one at a time, from the most relevant to the least relevant. For each of the classifiers generated, we evaluated its performance by computing the AUC score, specificity and sensitivity for the OOB data. Based on this analysis, we selected the top important predictor variables that optimized model’s performance and ran the training process again, generating a simplified RF classifier that considers only the most impactful variables.
Finally, we explored the relevance of variables for classification results by partial dependence plots, which are useful for providing insights of the marginal effect of a given variable over the desired outcome. The partial dependence of a variable’s effect is best understood by examining general patterns in relation to the values of the predictor variable rather than the specific values of partial dependence . Because we are modeling binary classification (i.e., presence/absence of BVDV), partial dependence values are given in “logit” scale and are computed in relation to the probability for the positive class .
Model performance assessment
Comparing RF to other machine learning methods
In order to assess the predictive power of RF in comparison to other ML techniques, we performed a comparative evaluation of the RF classifier with two other popular methods, namely Support Vector Machine (SVM) and Gradient Boosting Machine (GBM), which have also not been extensively assessed in veterinary epidemiology. SVM was introduced by  and is based on a statistical-learning technique known as structural risk minimization [41,42], being first used in observational epidemiology studies in 2005 . GBM, on the other hand, is an ensemble method that combines regression trees with weak individual predictive performances into a single model with high performance [34,40].
For such comparison, we adopted the same procedure used for RF training, i.e., 10 repetitions of 10-fold cross-validation, assuring that the exact same data points are used in each step of model training and testing. In other words, we maintained the same subsampling of the training data used in the cross-validation process. In addition, we applied the caret R package to train SVM and GBM models, tuning some of the parameters involved in order to carry a fair comparison with RF. Based on the results from cross-validation, we performed a first round of comparison among models, contrasting their AUC score, sensitivity and specificity drawn from the average confusion matrix. Finally, the differences between models performance in terms of AUC scores were assessed with a pairwise Wilcoxon rank test in order to test for statistical significance.
Comparing RF to logistic regression
Since we are interested in suggesting the use of RF as an alternative method for traditional statistical approaches, we also assessed its performance relative to logistic regression, which is frequently used for the analysis of risk factors. Logistic regression was estimated with the glm() function in R environment and performance evaluation was carried out based on 10 repetitions of 10-fold cross-validation using the caret R package. To assure a fair comparison, we run the logistic regression analysis with the same distribution of data used for RF training among folds and across all repetitions of cross-validation.
Models evaluation on independent testing data
In addition to evaluating the methods performance using cross-validation, we also assessed their predictive accuracy with an independent test set derived from the original data. As aforementioned, during data preparation the original data set was subdivided in training data (80%) and testing data (20%), which is not used in the cross-validation procedure and thus can be regarded as an independent test set.
This approach is recommended when no external independent data are naturally available [44,45], which is the case in our study. Although cross-validation is well known for providing precise and unbiased estimative of the predictive accuracy and generalization power of ML classifiers, we opted to follow the common practice and conduct another comparison among models with explicitly independent data.
Performance of the RF model
Classification performance of RF model for the 40 variables. Confusion matrix for the RF model trained with the complete set of predictor variables ( n = 40) and a down-sampling procedure, estimated by averaging the results over ten repetitions of 10-fold cross-validation. Standard deviations are given in parenthesis *
Analysis of the correlation matrix computed for the set of 40 variables (Additional file 3) suggested that a small set of independent variables is highly correlated. Based on plausibility criteria, we eliminated the highly correlated variables, namely (5) frequency of technical assistance, (9) frequency of veterinary assistance, (11) what is the origin of the animals brought into the farm and (30) has the farmer seen weak calves, and repeated the training process. We observed a minimal change in the RF model performance after the elimination of correlated variables, with the highest (but still modest) impact found for sensitivity, i.e., an increase from 62.26% to 65.10%.
Classification performance of RF model for the top 25 variables. Confusion matrix for the RF model trained with the top 25-predictor variables selected after variable importance analysis, estimated by averaging the results over ten repetitions of 10-fold cross-validation. Standard deviations are given in parenthesis *
As this analysis suggests, (B) the number of neighboring farms that have cattle and (G) the number of different inseminators in the last year had a strong linear correlation with BVDV. Moreover, we observed that disease occurrence was highly influenced by observations related to some specific variables, mainly by (A) insemination performed by the owner or farmer, (C) milk production representing about 61-80% of far income, (E) technical assistance conducted annually, (F) rectal palpation performed routinely, (I) veterinary assistance held annually, (J) animals placed in quarantine before introduction, (M) milking process performed in an automatic fashion, (X) administration of medications performed by a technician and (Y) the regional habit of injecting blood from a sick animal into a healthy one (“Premunição”). In contrast, there was no significant relationship between BVDV occurrence and the variables (O) does calving occurs in closed barns, (P) number of cows lactating at the sampling moment, (S) do animals share the same feed and water containers, (T) number of cows not lactating at the sampling moment, (U) is colostrum stock available and (W) are paddocks available for sick animals.
Comparative evaluation of RF, SVM and GBM
The results of the comparative analysis based on the average AUC scores, computed as the mean of the area under the ROC curves over all repetitions of cross-validation, were 0.702 for RF, 0.690 for GBM and 0.687 for SVM. The highest specificity was achieved by SVM (69.45% ± 4.05), followed by RF (67.65% ± 3.85) and GBM (66.15% ± 2.58). On the other hand, RF achieved the highest sensitivity (62.26% ± 3.44), followed by GBM (61.73% ± 5.33) and SVM (57.60% ± 4.73).
We also compared the distribution of sensitivity and specificity metrics across all repetitions of cross-validation following the same methodology, and we found that SVM has better specificity performance than RF and GBM (P-value < 0.05), while both RF and GBM outperform SVM in terms of sensitivity (P-value < 0.05).
Comparison between RF and logistic regression
As expected according to our theoretical motivation, we observed a superior performance of RF relative to logistic regression. While RF had an average AUC score of 0.702, the model estimated by logistic regression achieved an AUC score of 0.610 across all repetitions of cross-validation. The density plots drawn from the cross-validation procedure makes evident the better predictive power of RF, which presents an AUC scores distribution shifted to the right of that related to logistic regression (Figure 6B).
Moreover, we observed that the classification provided by RF is much more balanced in terms of sensitivity and specificity than logistic regression. The average specificity was 67.65% (±3.85) for RF and 61.36% (±3.33) for logistic regression, while the average sensitivity achieved by these methods were 62.26% (±3.44) and 56.30% (±3.84) for RF and logistic regression, respectively.
Models evaluation with independent testing data
Differently from the cross-validation technique that ensures every instance in the data set will be used exactly once for model validation, the initial partitioning of data is performed a single time in a random fashion, and may generate a testing data set for which GBM, fortunately, have a superior performance – an effect that is out of our control. To test for this possibility, we repeated the process of model training and testing 10 times, each of which with a random (and thus potentially different) partitioning of data into training and testing sets, keeping the proportions of 80% and 20%, respectively. We performed this procedure for the three classifiers, i.e., RF, SVM and GBM, and compared their average performance for the independent test data across all repetitions. We observed that RF outperforms the other classifiers in 6 out of the 10 repetitions, while in the remaining 4 the best performance is achieved by GBM (Additional file 4). Although the average AUC score of RF is only slightly better than GBM, 0.7466 vs. 0.7301, the worst and best performances achieved by RF show a performance gain of 12.09% and 7.13% in relation to the worst and best models trained by GBM, respectively.
Regarding the comparative evaluation between RF and logistic regression, similarly to what was observed from the cross-validation procedure, RF presented a more robust performance for independent testing data in relation to logistic regression. The ROC curves are shown in Figure 7B, corroborating the better predictive accuracy of RF in contrast to logistic regression.
In this study, we trained a RF model based on cross-sectional data derived from an investigation for BVDV prevalence carried in Southern Brazil, aiming to identify important predictors for disease occurrence and to evaluate the predictive power of this machine learning model in this specific domain. To the best of our knowledge, this is one of the few studies in veterinary epidemiology that performs an investigation based on machine learning algorithms adopting a careful training process, which encompasses parameters optimization and a strategy to treat a severe class imbalance problem. In addition, it was also the first time that a comparative evaluation among RF, SVM and GBM models was held in this context, adopting appropriate methods for model tuning and a repeated 10-fold cross-validation technique.
Based on the classification results by RF, we noticed that our model’s performance has shown an overall good predictive accuracy and quite balanced sensitivity and specificity across all repetitions of the cross-validation. The data-driven analysis carried by RF, without a priori assumptions about the relationship between the dependent and independent variables, has a great potential to outperform the traditional logistic regression, as experimentally verified for our data, suggesting that RF could be a valuable tool in cross-sectional studies. The reader should be aware that our results do not come from basic measures of total classification accuracy and error rates; instead, we have adopted robust evaluation approaches and made important interventions for training and optimizing the machine learning classifiers, providing a more appropriate application of these methods to our scenario. Specifically, we have optimized the number of predictor variables selected for splitting a new node during the production of the decision trees, and we decided to not optimize the number of trees in the forest based on the former discussion that RF is not very sensitive to this last parameter .
Despite its satisfactory performance, our classifier has missed on average more positive than negative cases of BVDV, even after the application of the down-sampling strategy (Table 1). Most standard algorithms assume or expect balanced class distribution or equal misclassification costs , so when a complex imbalanced data set is used, these algorithms fail to properly represent the distributive characteristics of the data and resultantly provide unfavorable accuracies across the classes of the data . In our data we found an imbalance in a form commonly referred to as “intrinsic”, which means the imbalance is a direct result of the nature of the data space . We analyzed the effects of the down-sampling procedure over classifiers performance, comparing the results obtained from training with and without handling the data imbalance issue, and we observed that all three methods suffered impact from the severe data imbalance over their sensitivity. When training is carried without treating this issue, models’ sensitivities were in the approximate range of 11.5% to 20%, which is clearly lower than the values of 57.60%, 61.73% and 62.26% achieved by SVM, GBM and RF, respectively. Hence, we observed that adopting this pre-processing strategy in data sets containing classes that are highly under-represented in comparison to others may introduce important benefits for data analysis, although in this case it did not completely solved this issue.
The final variables ranking in a descending order of importance as provided by RF’s variable importance analysis (Figure 3) suggests that the main variables involved in BVDV prediction are related to reproduction-associated factors, movement of many people into and out of the farms, and direct contact among animals, as we discuss further. Feature selection has been previously shown to result in slight error reductions , and this step is normally performed in order to remove variables that do not contribute to the performance of the model, either because they do not play an important role on error reduction or because they have a minimal effect on the discriminant power of the RF classifier . One can notice that although performance improvement was not so expressive after feature selection (Table 2), we still observed a slight gain in terms of accuracy and specificity. The top 25 variables model is therefore more efficient, as it provides a performance as good as the model trained with the complete set of 40 variables despite the reduction in model complexity.
Regarding the results of variable importance analysis, we discuss only the most relevant variables due to space limitation. The most impactful variable for BVDV prediction was related to farms that perform AI (Figure 5A), a factor that has been considered a predictor for BVDV globally, especially when semen is used from untested bulls or when farms use AI along with natural mating in order to “guarantee” the success of a pregnancy, a common and unsafe practice in Brazil . AI is an important route of transmission of BVDV because semen remains infective, which is evident by the demonstration that susceptible cows can become infected following insemination [15,49-51]. A remarkable new association that we found was that when AI is performed by the owner or someone that is responsible for the farm, a common reproductive practice in Brazil and other countries, the influence on BVDV cases was evidently harmful, increasing the probability of disease occurrence. It was also reaffirmed that the number of neighboring cattle farms where there is chances of direct contact between cattle over the fence was a predictor for BVDV . Others have identified the direct contact over fence lines one of the hardest to control . In our analysis, we showed that the partial dependence of BVDV on this variable increases as the numbers of neighbors’ increases, and that BVDV occurrence rises abruptly when there are three neighboring farms. The occurrence of BVDV was also influenced by factors related to milk production. When milk production was reported to represent 61 to 80% of farm income (Figure 5C), we observed a high association with BVDV, most likely due to milk production with intensive pressure on cow performance. It was found that farms that have produced milk for up to nine years had the highest influence on disease occurrence in contrast to farms that have been harvesting milk for longer periods (Figure 5D) this fact may be related to the inexperience of the farmer.
Partial dependence analysis also suggested that rectal palpation performed routinely (Figure 5F) causes significant influence on BVDV occurrence. It has been found that indirect transmission of BVD virus can be spread by veterinary equipment such as nose tongs, needles and protective rubber gloves worn during rectal examination [53,54]. Others  had also reported that rectal palpation performed consecutively on different animals without proper hygiene (e.g., without replacing glove between animals) might play an important role in the transmission of BVDV. Moreover, the number of different inseminators that had visited the farm in the past year showed a linear influence on BVDV (Figure 5G). We observed that as the number of inseminators increases, the chances of predicting positive cases of BVDV were also higher, probably due to intense people movement acting as fomites.
In order to compare the RF model against other classifiers that have similar literature, a repeated 10-fold cross-validation was performed, averaging model accuracy measures over all repetitions. We found a better overall performance of RF in relation to SVM and GBM, especially in terms of specificity and sensitivity balance, but results were very close among ensemble-based algorithms (i.e., RF and GBM). Although the difference between the AUC scores of these two classifiers are not statistically significant, we found based on visual analysis of kernel density estimates that the probability distribution of RF is shifted to the right of GBM and SVM distributions, which suggests that RF has a tendency to produce higher AUC scores (i.e., achieve best performance) in relation to the latter. Others had previously found similar results when testing the performance of all tree classifiers, but in the previous study, GBM and SVM performed relatively better than RF . The poor results related to SVM may be due to the fact that the performance and prediction results of this classifier are heavily dependent on the chosen values for the tuning parameters [57-59]. Although we adopted a parameter optimization procedure based on grid search methods that minimize total error rates, a more exhaustive study towards the evaluation of classifier’s performance upon parameters optimization, combined with the application of other optimization techniques, could lead to an even better performance. However, this analysis is out of the scope of our work.
Surprisingly, for tests with independent data, GBM showed an improved performance, which is better and more balanced than the performance achieved by RF and SVM. This may indicate a better generalization power of this algorithm, but it may also be an artifact of data partitioning, which randomly generates a test set for which GBM has a more favorable chance of producing accurate classification. However, due to the random nature of the procedure, repeated partitioning of the original data into training and testing sets may produce results with large variability, both qualitative and quantitative, and consequently provide less consistent insights than the analysis performed with cross-validation. We verified this effect by repeating 10 times the complete training process, from data preparation (and consequently data partitioning) to models evaluation, based on which we observed significant variance in methods performance. Briefly, RF and GBM were always the top-performing classifiers, but in 6 out of the 10 repetitions, RF outperformed GBM, showing that the outcome of this comparison is highly dependent on initial data partitioning. Hence, we emphasize that the 10-fold cross-validation technique is more powerful in reducing overfitting and more precise for assessing the predictive power of machine learning methods, providing an unbiased estimative of how a classifier model will generalize to an independent data set.
It should be noted that GBM is functionally similar to RF because it creates an ensemble of trees and uses randomization during this process. This fact could support the similar results observed for these two methods. However, whereas RF builds the trees in parallel and these trees “vote” simultaneously on the preferred class during prediction, GBM creates a series of trees in which the prediction receives incremental improvement by each tree in the series .
In life sciences, random forests have been used to analyze genomic data [61,62], in ecology they have been successfully used as classifiers [19,63,64], and herein they are used for cross-sectional studies in veterinary epidemiology. Random forests proved to have good accuracy, sensitivity and specificity, showing a discriminant power that is highly competitive with other ML-based methods for detecting biologically plausible predictors of BVDV. Based on these results, we believe that RF is a promising computational approach for cross-sectional studies in veterinary epidemiology and should be more frequently considered as an alternative for traditional statistical methods.
Moreover, our model demonstrated a novel use of observational data that goes beyond the previously identified predictors. The application of machine learning extends the usefulness of classical risk factors found on the basis of traditional statistical approaches. Based on the proposed RF model, we could take a closer look at some classical predictors and found important details regarding their relationship with disease occurrence, mainly regarding reproduction management, which should be considered for disease control and eradication. One should take this investigation further ahead in order to clarify how the important reproduction variables contribute to BVDV in other countries.
We thank the field team who carried out all the bulk milk tank sampling and questionnaire interviews. Part of this study was supported by FUNDESA and CNPq. MRM acknowledges the financial support from CAPES and HCPA.
- Simmonds P, Becher P, Collet MS, Gould EA, Heinz FX, Meyers G, Monath T, Pletnev A, Rice CM, Stiansny K, Thiel HJ, Weiner A, Bukhet J (2011) Family Flaviviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses 2011, 9th edn. Elsevier Academic Press, United States, pp 1003–1020Google Scholar
- Houe H (1999) Epidemiological features and economical importance of bovine virus diarrhoea virus (BVDV) infections. Vet Microbiol 64:89–107PubMedView ArticleGoogle Scholar
- Niza-Ribeiro J, Pereira A, Souza J, Madeira H, Barbosa A, Afonso C (2005) Estimated BVDV-prevalence, −contact and -vaccine use in dairy herds in Northern Portugal. Prev Vet Med 72:81–85PubMedView ArticleGoogle Scholar
- Valle PS, Martin SW, Tremblay R, Bateman K (1999) Factors associated with being a bovine-virus diarrhoea (BVD) seropositive dairy herd in the Mùre and Romsdal County of Norway. Prev Vet Med 40:165–177PubMedView ArticleGoogle Scholar
- Presi P, Struchen R, Knight-Jones T, Scholl S, Heim D (2011) Bovine viral diarrhea (BVD) eradication in Switzerland--experiences of the first two years. Prev Vet Med 99:112–121PubMedView ArticleGoogle Scholar
- Humphry RW, Brülisauer F, McKendrick IJ, Nettleton PF, Gunn GJ (2012) Prevalence of antibodies to bovine viral diarrhoea virus in bulk tank milk and associated risk factors in Scottish dairy herds. Vet Rec 171:445PubMedView ArticleGoogle Scholar
- Rodrigo SL, Perea A, García-Bocanegra I, Jos AA, Vinicio JD, Ramos R, Carbonero A (2012) Seroprevalence and risk factors associated with bovine viral diarrhea virus (BVDV) infection in non-vaccinated dairy and dual purpose cattle herds in Ecuador. Trop Anim Health Prod 44:645–649View ArticleGoogle Scholar
- Sarrazin S, Veldhuis A, Méroc E, Vangeel I, Laureyns J, Dewulf J, Caij AB, Piepers S, Hooyberghs J, Ribbens S, Van Der Stede Y (2012) Serological and virological BVDV prevalence and risk factor analysis for herds to be BVDV seropositive in Belgian cattle herds. Prev Vet Med 108:28–37PubMedView ArticleGoogle Scholar
- Gard JA, Givens MD, Stringfellow DA (2007) Bovine viral diarrhea virus (BVDV): epidemiologic concerns relative to semen and embryos. Theriogenology 68:434–442PubMedView ArticleGoogle Scholar
- Chaves NP, Bezerra DC, Sousa VE, Santos HP, Pereira HM (2010) Frequency of antibodies and risk factors of bovine viral diarrhea virus infection in non-vaccinated dairy cows in the Maranhense Amazon region, Brazil. Ciên Rur 40:1448–1451View ArticleGoogle Scholar
- Luzzago C, Frigerio M, Piccinini R, Dapra V, Zecconi A (2008) A scoring system for risk assessment of the introduction and spread of bovine viral diarrhoea virus in dairy herds in Northern Italy. Vet J 177:236–241PubMedView ArticleGoogle Scholar
- Lindberg AL, Alenius S (1999) Principles for eradication of bovine viral diarrhoea virus (BVDV) infections in cattle populations. Vet Microbiol 64:197–222PubMedView ArticleGoogle Scholar
- Machado G, Egocheaga RMF, Hein HE, Miranda ICS, Neto WS, Almeida LL, Canal CW, Stein M, Corbellini LG: Bovine Viral Diarrhoea Virus (BVDV) in dairy cattle: a matched case–control study. Transbound Emerg Dis (in press)Google Scholar
- Mainar-Jaime RC, Berzal-Herranz B, Arias P, Rojo-Vazquez FA (2001) Epidemiological pattern and risk factors associated with bovine viral-diarrhoea virus (BVDV) infection in a non-vaccinated dairy-cattle population from the Asturias region of Spain. Prev Vet Med 52:63–73PubMedView ArticleGoogle Scholar
- Almeida LL, Miranda ICS, Hein HE, Santiago NW, Costa EF, Marks FS, Rodenbusch CR, Canal CW, Corbellini LG (2013) Herd-level risk factors for bovine viral diarrhea virus infection in dairy herds from Southern Brazil. Res Vet Sci 93:901–907View ArticleGoogle Scholar
- Casanova R, Saldana S, Chew EY, Danis RP, Greven CM, Ambrosius WT (2014) Application of random forests methods to diabetic retinopathy classification analyses. PLoS One 9:e98587PubMed CentralPubMedView ArticleGoogle Scholar
- Mansiaux Y, Carrat F (2014) Detection of independent associations in a large epidemiologic dataset: a comparison of random forests, boosted regression trees, conventional and penalized logistic regression for identifying independent factors associated with H1N1pdm influenza infections. BMC Med Res Methodol 14:99PubMed CentralPubMedView ArticleGoogle Scholar
- Breiman L (2001) Random forests. Mach Learn 45:5–32View ArticleGoogle Scholar
- Cutler RD, Edwards TC, Beard KH, Cutler KT, Gibson HJ, Lawler JJ (2007) Random forests for classification in ecology. Ecology 88:2783–2792PubMedView ArticleGoogle Scholar
- Breiman L (2001) Statistical modeling. The two cultures. Stat Scie 16:199–231View ArticleGoogle Scholar
- Benito GM, Blazek R, Neteler M, Sánchez DR, Sainz-Ollero H, Furlanello C (2006) Predicting habitat suitability with machine learning models: the potential area of Pinus sylvestris L. in the Iberian Peninsula. Ecol Model 197:383–393View ArticleGoogle Scholar
- Peters J, De Baets B, Verhoest NEC, Samson R, Degroeve S, Becker PD, Huybrechts W (2007) Random forests as a tool for ecohydrological distribution modelling. Ecol Model 207:304–318View ArticleGoogle Scholar
- Slabbinck B, De Baets B, Dawyndt P, De Vos P (2009) Towards large-scale FAMEbased bacterial species identification using machine learning techniques. Syst Appl Microbiol 32:163–176PubMedView ArticleGoogle Scholar
- Kampichler C, Wieland R, Calmé S, Weissenberger H, Arriaga-Weiss S (2010) Classification in conservation biology: a comparison of five machine-learning methods. Ecol Inform 5:441–450View ArticleGoogle Scholar
- Pino-Mejías R, Cubiles VMD, Anaya-Romero M, Pascual-Acosta A, Jordán-López A, Bellinfante-Crocci N (2010) Predicting the potential habitat of oaks with data mining models and the R system. Environ Model Softw 25:826–836View ArticleGoogle Scholar
- Larison B, Njabo KY, Chasar A, Fuller T, Harrigan RJ, Thomas TB (2014) Spillover of pH1N1 to swine in Cameroon: an investigation of risk factors. BMC Vet Res 10:55PubMed CentralPubMedView ArticleGoogle Scholar
- Barco L, Macin M, Ruffa M, Saccardim C, Minorello C, Zavagin P, Lettini AA, Olsen JE, Ricci A (2012) Application of the Random Forest method to analyse epidemiological and phenotypic characteristics of Salmonella 4,,12:i:- and Salmonella Typhimurium strains. Zoonoses Public Health 59:505–512PubMedView ArticleGoogle Scholar
- Holtkamp DJ, Lin H, Wang C, O’Connor AM (2012) Identifying questions in the American Association of Swine Veterinarian's PRRS risk assessment survey that are important for retrospectively classifying swine herds according to whether they reported clinical PRRS outbreaks in the previous 3 years. Prev Vet Med 106:42–52PubMedView ArticleGoogle Scholar
- Instituto Brasileiro de Geografia e Estatística (IBGE): Pesquisa pecuária municipal, efetivo dos rebanhos por tipo de rebanho, Brasil. http://www.sidra.ibge.gov.br. Accessed 13 Jan 2014
- Zoccal R, Assis AG, Evangelista SRM(2006) Distribuição geográfica da pecuária leiteira no Brasil. Embrapa Gado de Leite, Juiz de Fora, http://ainfo.cnptia.embrapa.br/digital/bitstream/item/65271/1/CT-88-Distribuicao-geografica-da-pecuaria.pdf. Accessed 10 Jul 2014
- Silva GS, Costa E, Bernardo FA, Groff FHS, Todeschini B, Santos DV, Machado G (2014) Cattle rearing in Rio Grande do Sul, Brazil. Acta Scient Vet 42:1215Google Scholar
- Breiman L, Friedman JH, Olsen RA, Stone CJ (1984) Classification and Regression Trees. Chapman & Hall/CRC, BelmontGoogle Scholar
- Mendoza RM, Fonseca GC, Loss-Morais G, Alves R, Margis R, Bazzan ALC, RFMirTarget (2013) Predicting Human MicroRNA Target Genes with a Random Forest Classifier. PLoS One 8:e70153PubMed CentralPubMedView ArticleGoogle Scholar
- Hastie T, Tibshirani R, Friedman J (2009) The Elements of Statistical Learning: Data Mining, Inference, and Prediction. Springer, New YorkView ArticleGoogle Scholar
- Liaw A, Wiener M (2002) Classification and regression by random forest. R News 2:18–22Google Scholar
- Kuhn M (2014) caretClassification and Regression Training. http://cran.r-project.org/web/packages/caret/index.html. R package version 5.15–052, Accessed 20 Dec 2014
- Nguyen GH, Bouzerdoum A, Phung SL (2009) Learning pattern classification tasks with imbalanced data sets. In Pattern Recognition, Vukovar, Croatia, pp 193–208Google Scholar
- Xi C, Ishwaran H (2012) Random forest for genetic data analysis. Gonomics 99:323–329View ArticleGoogle Scholar
- Domingos P (2012) A few useful things to know about machine learning. Commu ACM 55:78–87View ArticleGoogle Scholar
- Friedman JH (2001) Greedy function approximation: a gradient boosting machine. Ann Stat 29:1189–1232View ArticleGoogle Scholar
- Vapnik V (1995) The nature of statistical learning theory. Springer, New York, p 314View ArticleGoogle Scholar
- Noble WS (2006) What is a support vector machine? Nat Biotechnol 24:1565–1567PubMedView ArticleGoogle Scholar
- Hermans PG, Fradkin D, Muchnik IB, Morgan KL (2006) Prevalence of wet litter and the associated risk factors in broiler flocks in the United Kingdom. Vet Rec 158:615–622PubMedView ArticleGoogle Scholar
- Efron B, Tibshirani R (1997) Improvements on cross-validation: the 632+ bootstrap method. J Am Statist Assoc 92:548–560Google Scholar
- Gerds T, Schumacher M (2007) Efron-type measures of prediction error for survival analysis. Biomet 63:1283–128View ArticleGoogle Scholar
- He H, Garcia AE (2009) Learning from Imbalanced Data. IEEE Trans Knowl Data Eng 21:1263–1284View ArticleGoogle Scholar
- Svetnik V, Liaw A, Tong C, Wang T (2004) Application of Breiman's Random Forest to modeling structure–activity relationships of pharmaceutical molecules. In: Roli F, Kittler J, Windeatt T (eds) Multiple Classifier Systems, Fifth International Workshop, MCS 2004, Proceedings, 9–11 June 2004, Cagliari, Italy, Lecture Notes in Computer Science, v. 3077. Springer, Berlin, pp 334–343Google Scholar
- Xiong C, Johnson D, Xu R, Corso JJ (2012) Random forests for metric learning with implicit pairwise position dependence. In: ACM SIGKDD International Conference on Knowledge Discovery and Data Mining, pp 958–966Google Scholar
- Altamiranda EA, Kaiser GG, Weber N, Leunda MR, Pecora A, Malacari DA, Morán O, Campero CM, Odeón AC (2012) Clinical and reproduction consequencs of using BVDV-contaminated semen in artificial insemination in a beef herd in Argentina. An Repr Scie 133:146–152View ArticleGoogle Scholar
- Kirkland P, Mackintosh S, Moyle A (1994) The outcome of widespread use of semen from a bull persistently infected with pestivirus. Vet Rec 135:527–529PubMedView ArticleGoogle Scholar
- Paton DJ, Brockman S, Wood L (1999) Insemination of susceptible and preimmunized cattle with bovine viral diarrhoea virus infected semen. Bra Vet J 146:171–174View ArticleGoogle Scholar
- Niskanen R, Lindberg A (2003) Transmission of bovine viral diarrhoea virus by unhygienic vaccination procedures, ambient air, and from contaminated pens. Vet J 165:125–130PubMedView ArticleGoogle Scholar
- Gunn HM (1993) Role of fomites and flies in the transmission of bovine viral diarrhoea virus. Vet Rec 132:584–585PubMedView ArticleGoogle Scholar
- Lang-Ree JR, Vatn T, Kommisrud E, Loken T (1994) Transmission of bovine viral diarrhoea virus by rectal examination. Vet Rec 135:412–413PubMedView ArticleGoogle Scholar
- Goyal SM, Ridpath JF (2005) Bovine Viral Diarrhea Virus Diagnosis, Management and Control. Blackwell, IowaView ArticleGoogle Scholar
- Ogutu JO, Piepho H, Schulz-Streeck T (2011) A comparison of random forest, boosting and support vector machines for genomic selection. BMC Proc 5(Suppl 3):S11PubMed CentralPubMedView ArticleGoogle Scholar
- Duin RPW (1996) A note on comparing classifiers. Pat Recog Lett 17:529–536View ArticleGoogle Scholar
- Meyer D, Leischa F, Hornik K (2003) The support vector machine under test. Neurocom 55:169–186View ArticleGoogle Scholar
- Lim TS, Loh WY, Shih YS (2000) A comparison of prediction accuracy, complexity, and training time of thirty-three old and new classification algorithms. Mach Lear 40:203–228View ArticleGoogle Scholar
- Olinsky A, Kennedy K, Kennedy BB (2014) Assessing gradient boosting in the reduction of misclassification error in the prediction of success for actuarial majors. Math Departt J Articl 5:12–16Google Scholar
- Jiang P, Wanf W, Ma W, Sun X, Lu Z (2007) MiPred: classification of real and pseudo microRNA precursors using random forest prediction model with combined features. Nucleic Acids Res 35(Suppl 2):W339–W344PubMed CentralPubMedView ArticleGoogle Scholar
- Wu J, Liu H, Duan X, Ding Y, Wu H, Bai Y, Sun X (2009) Prediction of DNA-binding residues in proteins from amino acid sequences using a random forest model with a hybrid feature. Bioinformatics 25:30–35PubMed CentralPubMedView ArticleGoogle Scholar
- Prasad AM, Iverson LR, Liaw A (2006) Newer classification and regression tree techniques: bagging and random forests for ecological prediction. Ecosystems 9:181–199View ArticleGoogle Scholar
- Drew CA, Wiersma Y, Huerrmann F (2011) Predictive modeling in landscape ecology. Springer, New YorkView ArticleGoogle Scholar
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