Columnaris disease in fish: a review with emphasis on bacterium-host interactions
© Declercq et al.; licensee BioMed Central Ltd. 2013
Received: 1 February 2013
Accepted: 10 April 2013
Published: 24 April 2013
Flavobacterium columnare (F. columnare) is the causative agent of columnaris disease. This bacterium affects both cultured and wild freshwater fish including many susceptible commercially important fish species. F. columnare infections may result in skin lesions, fin erosion and gill necrosis, with a high degree of mortality, leading to severe economic losses. Especially in the last decade, various research groups have performed studies aimed at elucidating the pathogenesis of columnaris disease, leading to significant progress in defining the complex interactions between the organism and its host. Despite these efforts, the pathogenesis of columnaris disease hitherto largely remains unclear, compromising the further development of efficient curative and preventive measures to combat this disease. Besides elaborating on the agent and the disease it causes, this review aims to summarize these pathogenesis data emphasizing the areas meriting further investigation.
Table of contents
- 1.The agent
History and taxonomy
Morphology and biochemical characteristics
- 2.The disease
Clinical signs, histopathology, ultrastructural features and haematology
Exotoxins, bacteriocins and endotoxins
Interaction with the fish immune system
Importance of environmental factors
Experimental trials/pathogenicity tests
1. The agent
1.1 History and taxonomy
Flavobacterium columnare (F. columnare), the causative agent of columnaris disease, belongs to the family Flavobacteriaceae[1–3]. Columnaris disease was first described by Davis among warm water fishes from the Mississippi River . Although unsuccessful in cultivating the etiological agent, Davis described the disease and reported large numbers of slender, motile bacteria present in the lesions . Upon examining a wet mount preparation of these lesions, column-like structures formed by these bacteria were evident. The organism was hence named Bacillus columnaris and the disease elicited columnaris disease. Over the decades the taxonomic status of the pathogen has changed several times since the pioneering work of Davis . In 1944, Ordal and Rucker were the first to isolate the bacterium from a natural outbreak of columnaris disease among sockeye salmon (Onchorhynchus nerka) . A diluted culture medium was used to grow the bacterium. Based on cellular morphology, they identified the bacterium as a myxobacterium. Organisms classified in the order Myxobacteria are long, thin Gram-negative rods that are motile on agar media by a creeping or flexing motion. They have a life cycle composed of vegetative cells, microcysts (resting cells), and fruiting bodies, or only vegetative cells and microcysts . Ordal and Rucker reported that the myxobacterium from columnaris disease produced both fruiting bodies and microcysts and named the organism Chondrococcus columnaris. Garnjobst assigned the bacterium to the family Cytophagaceae as Cytophaga columnaris after isolating a pathogenic bacterium resembling Chondrococcus columnaris morphologically, but not producing microcysts . Bernardet and Grimont reclassified the organism and placed it in the family Cytophagaceae and the genus Flexibacter, assigning it as Flexibacter columnaris. Finally, in 1996, the bacterium received its current name, Flavobacterium columnare, based on DNA-rRNA hybridization data and protein and fatty acid profiles . In 1999, the F. columnare cluster was subdivided in three genomovars based on differences in 16S rRNA sequences, restriction fragment length polymorphism (RFLP) and DNA-DNA-hybridization .
1.2 Morphology and biochemical characteristics
Long, slender gliding rods of 4 to 10 μm and 0.3 to 0.5 μm wide. In aging cultures spheroplasts may occur
Congo red absorption
Present due to an extracellular galactosamine glycan in the mucus and the production of flexirubin-type pigments
Degradation of crystalline cellulose
Degradation of complex acidic polysaccharides of connective tissue
F. columnare is distributed worldwide in fresh water sources and may infect many different wild and cultured freshwater fish species, such as (but not limited to) carp, channel catfish, goldfish, eel, perch, salmonids and tilapia [1, 12–17]. This disease also assails many tropical freshwater aquarium fish [12, 14]. In the channel catfish (Ictalurus punctatus) industry in the United States, F. columnare is the second most prevalent bacterium, after Edwardsiella ictaluri, to cause disease and mortality [18, 19], with yearly losses estimated at 30 million dollars . This organism can also be part of the bacterial microbiota of freshwater fish, eggs and the rearing waters the fish live in . Fish may reside in a clinically healthy carrier status harbouring an isolate remaining from a previous outbreak of columnaris disease and in this way act as an infection source for other fish [22, 23]. Fujihara and Nakatani reported that rainbow trout surviving a F. columnare infection can release up to 5 × 103 colony forming units/mL/h of viable bacteria into tankwater . The gills were shown to be the major release site of this pathogen. Dead fish would be able to spread the disease at a higher transmission rate compared to living fish .
Several studies have indicated the potential for F. columnare to survive for extended periods in water. Survival was demonstrated to be influenced by physical and chemical characteristics of the surrounding water. Fijan indicated that F. columnare can survive up to 16 days at 25°C in hard, alkaline water with a high organic load . Soft water with 10 mg/L CaCO3, especially when acid or with a low organic content, does not provide a favorable environment for the organism . Chowdhury and Wakabayashi determined that calcium, magnesium, potassium and sodium ions all are important for long-term survival of F. columnare in water . Ross and Smith found that survival of F. columnare in static, sterile river water was directly related to temperature, with a higher survival percentage at lower temperatures . The bacterium can keep its infectivity in lake water in laboratory conditions for at least five months . F. columnare is also capable of surviving in sterile river mud . Apparently, mud slurry often contains sufficient nutrients to maintain viability of F. columnare longer than sterile river water. In this case, however, the percentage survival of F. columnare seeded into mud seems to be higher at 25°C than at 5°C. Temperatures below 5°C are even detrimental to F. columnare cells in mud. F. columnare also grows well on particulate fish feed . When surviving outside the host, F. columnare can change from a virulent to a less virulent form with an altered colony morphology, probably to save energy . It has been suggested that F. columnare strains at fish farms originate from environmental waters and that the farm environment and practices may select for virulent strains that cause outbreaks in the farm [28, 30].
2. The disease
2.1 Clinical signs, histopathology, ultrastructural features and haematology
Ulceration of the oral mucosa also occurs, resulting in mouthrot. These mouth lesions are more lethal than are the skin lesions, since the painful oral lesions render the fish anorectic and lead to death due to starvation. Moreover, the disease spreads easily to the mandible and the maxilla. Secondary infections with fungi or other bacteria may deteriorate the situation and can be seen together with the filamentous bacteria . In tropical fish, this clinical sign led to the disease being termed “cotton wool disease” or “mouth fungus” . Finrot can also be present in tropical fish . Lesions can be restricted to local skin discoloration, with or without ulceration, and degeneration of underlying muscle fibers [1, 12, 39].
The skin or gills need to be abraded, for bacteria to enter the bloodstream and cause systemic infections . However, Hawke and Thune have isolated F. columnare from internal organs without any external lesions appearing . Foscarini described that the pathological changes to the gill structure caused by columnaris disease go hand in hand with cardiac alterations . The first day after infection, bradycardia was noticed with the formation of hyperplastic gill lesions. Degenerative processes of the lamellae in the following days resulted in a compensatory tachycardia. This research suggests that the interaction between the impaired gill vascular blood circulation and the cardiac changes could result in the death of the fish .
Columnaris disease may cause acute ulcerative dermatitis extending into the hypodermis and the muscle. Waterlogging can be present. The latter appears when the osmotic barrier is broken and thus forces water into the tissues, leading to severe dermal edema. Rupture of pigment cells with the loss of melanocytes can also be seen. Columns or hay-stack-like aggregates of bacteria can be gathered between dermal collagen fibers. The typical long and slender bacterial cells can easily be noted upon inspecting hematoxylin and eosin (H&E) or Giemsa stained sections from affected tissue where they appear bluish-purple and blue, respectively . When all these changes occur rapidly, they may proceed to severe necrosis and sloughing of the epidermis [32, 38].
Bullard and McElwain were the first to describe the ultrastructural features of saddleback lesions associated with experimental infections of F. columnare in channel catfish and zebrafish (Danio rerio) using SEM . Channel catfish skin lesion samples had margins typified by shed epidermal cells and lesion centers that exhibited a multitude of rod-shaped bacterial cells, approximately 3-10 μm long and 0.3-0.5 μm wide, intermixed with cellular debris. Zebrafish skin lesion samples displayed a multitude of rod-shaped bacterial cells and exhibited similar ultrastructural changes. Scales were missing or, when present, denuded of epidermis.
In a cutaneous columnaris challenge model in koi carp, significant changes in blood parameters were observed in the infected fish . For the hematologic parameters, a significant decrease was noted in Packed Cell Volume (PCV), haemoglobin concentration, red blood cell count, mean corpuscular volume and absolute lymphocyte counts. As for the biochemical parameters, marked hyponatremia, hypochloridemia and hyperglycemia were observed. Calcium and magnesium levels dropped only slightly and total serum protein and albumin-like protein concentrations decreased mildly. Alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and creatine kinase (CK) secretions were significantly increased . In a study by Řehulka and Minařík blood parameters from brook trout suffering from an acute, natural outbreak of F. columnare also revealed anemia, but levels of mean corpuscular volume and mean corpuscular haemoglobin were higher . Total protein levels fell much below physiological parameters in infected fish. Calcium concentrations were reduced significantly. Blood Urea Nitrogen (BUN) measurements were much higher compared to normal levels. The catalytic activity of AST, alanine aminotransferases and LDH reached multiples of normal values. In contrast, ALP concentrations decreased. Hypoglycemia was noted. No data were shown on sodium or chlorine .
Timely detection of this pathogenic agent is important to prevent its spreading and to reduce the economic loss to fish farmers.
The isolation of F. columnare is possible from external lesions, provided that the samples are taken from the edge of recent lesions . F. columnare requires low nutrient media. No growth of F. columnare is reported on trypticase soy agar (TSA), nutrient agar or Marine 2216 agar . In 1944, F. columnare was first cultured on Cytophaga agar, a nutrient poor medium . Since then, several media, including Shieh  and TYES  have been developed in an attempt to improve growth of the bacterium. Growth does not occur in media that contain NaCl concentrations of over 0.5% or that have a pH lower than six . Depending on the strain, the bacterium grows between 15 and 37°C [9, 12], with an optimal growth occurring between 25°C and 30°C. Colonies appear after 24 to 48 h of incubation . Glucose does not improve growth. Considering that F. columnare is easily overgrown by contaminating bacteria, selective media have been developed based on inherent resistance of F. columnare to different antimicrobial agents, including polymyxin and neomycin [6, 8, 25]. Decostere et al. added one μg/mL tobramycin to modified Shieh medium, demonstrating this to be an effective selective supplement for isolating F. columnare.
F. columnare largely displays two colony types on solid media; smooth and rhizoid . Kunttu et al. characterized a third, rough colony morphology variant . The rhizoid colony variants were assigned virulent and moderately adherent, the non-rhizoid rough colony variants non-virulent and highly adherent, and the smooth colony variants non-virulent and poorly adherent . Colonies of F. columnare are notorious for their sometimes strong adherence to the agar. This trait is also exhibited in broth cultures where yellow, filamentous clumps of bacterial cells can form a thick ring at the surface of a glass recipient [12, 50]. Adherence may be lost after several subcultures. Colonies can be recognized by their distinctive yellow pigmentation. The yellow color is due to the production of flexirubin pigments . A method to identify the typical protein profile of F. columnare is whole-cell protein analysis . Genomovar ascription has been performed using 16S-restriction fragment length polymorphism to divide F. columnare into three genomovars I-III . Compared to RFLP, single strand conformation polymorphism (SSCP) would have an improved resolution power in the study of intraspecies diversity in F. columnare. Polymerase chain reaction (PCR) has gained interest for definitive identification of F. columnare based on the selective amplification of the 16S ribosomal RNA gene using species-specific primers [52, 53]. This technique gives a conclusive identification of the organism within a few hours and eliminates the need for biochemical testing which is laborious and sometimes inconclusive .
Besides isolation, other methods may be used for detecting this pathogen. These include serological methods such as the enzyme-linked immunosorbent assay  and the fluorescent antibody test [55, 56]. Both have proven to be efficient and rapid for diagnosing columnaris disease. A loop-mediated isothermal amplification method (LAMP) for rapid detection of Flavobacterium columnare from infected fish organs (gills, skin and head kidneys) was established in channel catfish . PCR may also be adopted, having the advantage of being able to detect very low levels of F. columnare[52, 53]. In addition, Panangala et al. have developed a TaqMan-based real-time PCR targeting a 113 bp nucleotide region of the chondroitin AC lyase gene of F. columnare. This PCR is specific, sensitive and reproducible for the detection and quantitation of F. columnare in tissues (blood, gills and kidney) of infected fish. Moreover, real-time PCR-based methods are distinctively more advantageous than conventional PCR as they eliminate the need for detection of amplified products by gel electrophoresis, thus reducing costs, time and labour . Length heterogeneity PCR (LH-PCR) is based on comparing naturally varying lengths of 16S rDNA PCR products between bacterial groups . LH-PCR has also proven effective in detecting F. columnare in fish tissue .
Various reports have noted the difference in virulence among F. columnare strains [11, 12, 40, 42, 61–65]. In the last decade, various studies have attempted to elucidate the pathogenesis of columnaris disease. Although significant progress has been made, still many questions remain unanswered on how this pathogenic organism elicits disease, and information concerning the bacteriological events preceding disease and death is scarce. Li et al. related this lack of knowledge to the absence of an efficient molecular manipulation system for F. columnare, especially a plasmid-based inframe knockout system , despite two recent reports on the establishment of genetic manipulation system for the bacterium [67, 68]. Li et al. identified the type I restriction-modification system (R-M system) in F. columnare to improve electroporation efficiency and suggested that it would be of significant interest to examine the composition and diversity of R-M systems in strains of F. columnare in order to set up a suitable genetic manipulation system for the bacterium . Only very recently, the full genome of F. columnare ATCC 49512 was sequenced .
The colonization of the fish tissue is to be regarded as a complex multistep process which can be subdivided into the stages of attraction, adhesion and aggregation, requiring a step-by-step analysis. The exact factors mediating colonization have however not yet received the full attention they merit and to date remain largely unidentified.
The research group of Klesius et al. demonstrated by means of the traditional capillary tube method that the mucus from the skin and gills of catfish promotes chemotaxis of F. columnare. The gliding motility of this bacterium is well known [1, 12, 41]. Indeed, the observation of a drop of bacteria grown in broth under a phase contrast microscope shows the slowly forward and backward gliding of F. columnare. Pate and Ordal described that the location of fibrillar structures spanning the gap between the outer membrane and the mucopeptide layer might play a role in the gliding motility of the F. columnare bacterial cells . Although Klesius et al. acknowledged that they were not able to fully define the role that chemotaxis plays in the virulence of F. columnare, the chemotactic response of the more virulent genomovar ll isolates suggested that chemotaxis could be associated with virulence . LaFrentz and Klesius developed a culture independent method to quantify the chemotactic response of F. columnare to skin mucus using blind-well chemotaxis chambers  generating similar results as stated above . At least three carbohydrate-binding receptors (D-mannose, D-glucose and N-acetyl-D-glucosamine) associated with the capsule of F. columnare might be involved in chemotactic responses . The gliding motility gene gld H was found to be significantly (P < 0.001) upregulated in F. columnare as soon as five minutes post-exposure to catfish mucus. When pretreated with D-mannose, there was no upregulation of gliding motility genes .
The ability to adhere is a prerequisite for the successful colonization of the host tissue. Decostere et al. performed a series of both in vivo and in vitro experiments and found that a highly virulent strain adhered more readily to the gill tissue of black mollies (Poecilia reticulata) than did the low virulence strain [11, 12, 42]. This research group underscored that the adhesion of F. columnare to the gill tissue constitutes an important step in the pathogenesis of columnaris disease. Bader et al. adopted an adhesion defective mutant of F. columnare in immersion challenge trials and found that the mortality was reduced with 75% and occurred 24 h later compared to the strains that still possessed the adhesion capacities, confirming the findings of the former research group . In channel catfish genomovar II is considered to be more virulent than genomovar I [51, 72]. Challenge of rainbow trout with genomovar I and II isolates of F. columnare demonstrated a difference in the cumulative percent mortalities (CPM), with the genomovar II isolates inducing significantly higher CPM . Olivares-Fuster et al. compared adhesion of F. columnare genomovar I and II strains to the skin and gill of channel catfish and the gill of zebrafish (Danio rerio) . At 0.5 h post-challenge, both strains adhered to the gill of channel catfish at comparable levels, but significant differences in adhesion were found in later datapoints over time. They concluded that particular strains of F. columnare exhibit different levels of adhesion to their fish hosts and that adhesion to fish tissues is not sufficient to cause columnaris disease. The same statement was previously made by Klesius et al. .
Kunttu et al. have shown that colony morphology affects the adhesion capacity of F. columnare to polystyrene and put forward the hypothesis that there is a link between virulence and rhizoid colony morphology [49, 74]. The formation of different colony morphologies could be caused by changes in the cell surface components of the bacteria. It was also found that F. columnare changes colony morphology during experimental long-term storage in lake water, indicating the importance of different colony morphologies to bacterial survival [64, 74, 75]. Arias et al. did note that long-term starvation appears to decrease cell fitness and resulted in loss of virulence . However, whether adhesion impairment was the cause of the observed virulence loss was not investigated.
F. columnare produces two types of mucus. The first one is an acidic polysaccharide and is made visible by ruthenium red staining. Another type of mucus is a basic, partially acetylated polygalactosamine, which cannot be stained with ruthenium red. Pate and Ordal described a capsular material which coated the surface of the bacterial cell that could be stained with ruthenium red . They stated that the ruthenium red-positive material was probably an acid mucopolysaccharide that might be involved in the adhesive properties of the cells. The exact role of the mucopolysaccharides was however not further illuminated in this study. Decostere et al. demonstrated that the adherence capabilities of a highly virulent F. columnare strain to the gill were significantly reduced following treatment of the bacteria with sodium metaperiodate or incubating them with D-glucose, N-acetyl-D-glucosamine, D-galactose and D-sucrose . Treatment with pronase or trypsin did not cause any significant inhibition of adhesion . The same research group noted that the highly virulent strain had a thick capsule with a regular and dense appearance, whereas the capsule of the low virulent strain was much thinner. This made them to speculate that a lectin-like carbohydrate substance incorporated in the capsule might be partially responsible for the adhesion to the gill tissue.
Sun et al. conducted the first transcriptomic profiling of host responses to columnaris disease following an experimental immersion challenge by using illumina-based RNA-sequencing expression profiling . A rhamnose-binding lectin (RBL) was detected as by far the most highly upregulated gene observed in their differentially expressed set, with expression increasing 105-fold by four hours following infection. This upregulation dramatically decreased at the later verified timepoints (24 h and 48 h), suggesting the importance of this gene during early infection events . Immunohistochemical staining with antisera against an RBL in rainbow trout revealed the presence of these RBLs in mucous cells of the gill and in various cells related to innate immunity . This expression pattern is consistent with the finding of F. columnare bacterial cell aggregates around goblet cells in common carp  and catfish  following experimental challenge with a highly virulent strain . Beck et al. identified two distinct catfish families with differential susceptibilities to columnaris disease, with the one family completely resistant and the other susceptible . In the susceptible family, an acute and robust upregulation in catfish RBL was observed following challenge that persisted for at least 24 h. After exposure of the catfish to different doses of the putative RBL ligands L-rhamnose and D-galactose, these sugars were found to protect the fish against columnaris disease, likely through competition with F. columnare binding of host RBL. Moreover, RBL expression was upregulated in fish fasted for 7 d (as compared to fish fed to satiation daily), yet expression levels returned to those of satiated fish within 4 h after re-feeding. These findings highlight putative roles for RBL in the context of columnaris disease and reveal new aspects linking RBL regulation to feed availability . Further studies are needed to further pinpoint the (various) factor(s) responsible for mediating adhesion to the fish tissue.
The adhesion to the fish tissue was shown to be impacted by various environmental parameters. Using a gill perfusion model [61, 80], Decostere et al. noted that the adhesion of a highly virulent strain to the gill tissue was enhanced by a number of factors, including immersion of the gill in divalent ion water, the presence of nitrite or organic matter, and high temperatures . The positive effect of high temperatures on the adhesion has also been demonstrated by Kunttu et al. . Adhesion seems to decrease in vitro as salinity goes up . Expression of adhesins by bacteria is regulated on two different levels. One is directed by environmental sensing and transcription of specific regulatory elements, and the other is a random switching on and off of adhesion genes by submission of the bacterial population to unpredictable environmental conditions . If and how both phenomena are established in F. columnare remains to be elucidated.
An aggregative adhesion pattern of a highly virulent F. columnare strain onto gill tissue is a distinct feature in both in vivo and organ culture experiments [35, 42, 53, 61]. This results in an irregular gill surface covered by a thick mat consisting of numerous clumps of F. columnare bacterial cells, most likely impeding oxygen uptake and causing death of the fish. These microcolonies are not observed when a low virulence strain is used. Biofilm formation capacity was demonstrated in vitro for one F. columnare strain when exposed to mucus . These features point towards biofilm formation potentially being an important stage in the pathogenicity of F. columnare, warranting its further investigation.
The developmental switch to the biofilm state is commonly regulated by quorum sensing. Quorum sensing (QS) is a mechanism of gene regulation in which bacteria coordinate the expression of certain genes in response to the presence or absence of small signal molecules. In many Gram-negative bacteria, the signal molecule is an N-acylhomoserine lactone (AHL) . Additionally, a signaling molecule known as autoinducer-2 (AI-2) may also be employed . Wagner-Döbler et al. found short-chain AHL-type activity in Flavobacterium sp., but no AHL-presence could be confirmed using gas chromatography-mass spectrometry (GS-MS) . Using light chromatography-mass spectrometry (LC-MS), Romero et al. described the presence of short-type AHL activity in the culture media of nine Tenacibaculum maritimum strains, biofilm-forming members of the phylum Bacteroidetes, formerly referred to as “Cytophaga-Flavobacterium-Bacteroides” group . To our knowledge, so far, QS by AHL or AI-2 has not yet been demonstrated in F. columnare.
3.2 Exotoxins, bacteriocins and endotoxins
It is known that polysaccharide degradation in combination with the secretion of various extracellular enzymes participate in the destruction of skin, muscle and gill tissue , enhancing pathogenicity. In culture, F. columnare produces an enzyme that degrades chondroitin sulfates A and C and hyaluronic acid, the complex polysaccharides of connective tissue. This so-called chondroitin AC lyase acts specifically on a group of acidic mucopolysaccharides found primarily in animal connective tissue . No correlations were found between host origin, geographic distribution, and amount of enzyme produced by different isolates . AC lyase is alleged to play a role in the virulence of F. columnare[49, 65]. Though high AC lyase activity solely would not be enough to induce virulence in F. columnare strains, both high AC lyase activity and gliding motility of the bacteria would be needed for F. columnare to be virulent . Proteases also contribute to damaging the tissue or enhancing invasive processes . Newton et al. isolated and partially characterized proteases of 23 isolates of Flavobacterium columnare derived primarily from channel catfish raised in the southeastern United States . The bacterial isolates were divided into two groups according to the apparent molecular masses of proteases after zymographic resolution by non-reducing, non-denaturing sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) with gelatin as the protease substrate. Isolates of group one, produced two proteases with apparent molecular masses of 53.5 and 58 kilodaltons. The isolates of group two, revealed three proteases with apparent molecular masses of 44.5, 48 and 59.5 kilodaltons. All isolates degraded gelatin and casein. Seven out of 23 isolates degraded elastin. More protease was produced in a medium with low nutrients and salts than in media with higher concentrations of nutrients. Moreover, a sharp increase was seen in protease production during the first 24 h of incubation and these levels dropped only slightly in the remaining days of the experiment .
Bacteria not only need to enter the host tissue, they also need to eliminate competitive bacteria. Different strains of F. columnare release specific, non-transmissible, bactericidal substances equivalent to colicins of Escherichia coli into the environment to reduce competition from other bacterial strains . It was postulated that cells of F. columnare also possess multiple specific receptors for the bacteriocins, and, consequently, the cells are sensitive only to those bacteriocins for which the cell possesses receptors . Antagonism of Pseudomonas sp. MT5 against F. columnare bacteria was found to be very strong in agar assays . However, antagonistic baths of the Pseudomonas bacterial strain could not yet prevent nor treat a F. columnare infection following experimental challenge .
Differences in LPS composition between highly and low virulent strains of F. columnare retrieved from channel catfish have been detected . Analysis of LPS by immunoblotting revealed that an avirulent mutant of a F. columnare isolate lacked the high molecular weight components of LPS present in virulent isolates. Based on these differences of LPS and total protein profiles, the research group was able to discriminate the attenuated mutant from other F. columnare strains . Kunttu et al. determined LPS-profiles of different colony morphology variants of F. columnare in rainbow trout . Colony morphology variants of the same strain produced a similar single LPS band. However, there were size differences between different strains. Both research groups used different LPS extraction and detection methods, rendering comparison of the obtained results impossible .
3.3 Interaction with the fish immune system
When encountering an infection, the first system activated is the innate immune system. It is assumed that the surface mucus layer, as a first physical-immunological barrier, plays an important adhesive role and that it is part of the innate host resistance of fish to disease . Antibacterial characteristics of the fish mucus against F. columnare have been demonstrated. In an experimental challenge trial of cutaneous columnaris disease in koi carp , lesions were noted on locations where mucus had been removed. After incubating F. columnare inoculated agar plates, a lower number of colonies was counted on plates to which mucus was added. Fluorescent microscopy of a stain-based bacterial viability assay also revealed a higher number of dead bacteria in F. columnare cultured with mucus . Staroscik and Nelson compared growth, biofilm formation, extracellular protease production and changes in protein expression of a highly virulent F. columnare strain cultured in media supplemented with juvenile Atlantic salmon (Salmo salar L.) skin mucus with the same media without mucus . Interestingly, and in contrast to the reasoning resulting from the above mentioned studies, salmon surface mucus promoted the growth of F. columnare, induced the bacteria to grow as a biofilm and increased extracellular protease activity . This might indicate that skin mucus of different fish species responds differently to the bacteria, or that it is the F. columnare strain which is critical in determining the antibacterial capacities of the mucus.
The fish skin in itself also forms a barrier against pathogens. Aranishi et al. immersed Japanese eel (Anguilla japonica) in a F. columnare suspension to monitor dermal nonspecific stress responses . They found that cathepsins B and L activities in the infected fishes increased more than 1.5-fold over their initial values over a 48 h period, along with a 4.5-fold increase in bacteriolytic activity. These cathepsins likely participate in bacteriolysis associated with Japanese eel skin and their activities may represent an important nonspecific response of eels .
Some studies reveal that F. columnare might be able to avoid parts of the immune system. In a study by Ourth and Bachinski, the catfish alternative complement pathway (ACP) was inhibited by large amounts of sialic acid contained by Gram-negative bacterial pathogens, including F. columnare. Sialic acid seemed to be the determining factor for the pathogenicity of F. columnare, as very little or no bactericidal activity was produced against this bacterium by the catfish ACP. Furthermore, the latter greatly increased after removal of sialic acid with neuraminidase . Another recurrent feature in F. columnare infections, is the lack of an inflammatory response as observed upon inspecting affected tissues microscopically. This resulted in the hypothesis that F. columnare triggers the endogenous programmed cell death machinery of immune cells to evade the immune system. Do Vale et al. have already proven that apoptosis can be a very powerful pathogenic strategy by inducing lysis of phagocytic cells . Sun et al. speculated that negative regulation of one of the central innate immune signaling pathways NF-κB, may be the result of immune evasion or manipulation by F. columnare via secreted toxins . Furthermore, high levels of inducible nitric oxide synthases (iNOS), apoptotic-promoting interferon (IFN) and other members of oxidative stress responses and apoptotic pathways, such as caspase 8 and G3BP1 (Rasputin), were observed following a F. columnare infection. Rasputin plays an inhibitory role in negative regulation of apoptosis and was highly downregulated at all examined time-points . This could explain why hardly any inflammatory cells appear in early infections with F. columnare as was described by Morrison et al. .
Ourth and Wilson demonstrated that F. columnare is resistant to the bactericidal action of serum of non-immunized catfish via the ACP . Ourth and Bachinski confirmed this finding , but also demonstrated that the classical, antibody-mediated complement pathway is highly effective in killing F. columnare. The virulence of F. columnare can also be influenced by transferrin . The survival time of fish experimentally challenged with F. columnare by intraperitoneal injection with iron-free human transferrin (Sigma) was reduced when iron was injected prior to exposure. The effect of iron was only evident in one of the two strains examined when the challenge was delivered via immersion. These results indicate that iron depletion may limit the virulence of F. columnare more in systemic infections than in external infections. This hypothesis is supported by data indicating that administration of transferrin prior to challenge increased survival after challenge by injection but had little or no effect on bath-challenged fish . However, the results found were not consistent for all strains tested and no statistical data were presented.
Several immunization experiments adopting different administration routes, have proven that fish can be protected from subsequent F. columnare infections by activating the adaptive immune system [20, 22, 95–98]. High agglutinin titers and good protection were obtained in trout following subcutaneous or intraperitoneal injection with heat-killed F. columnare cells . Schachte and Mora obtained a high agglutinin titer in channel catfish by intramuscular injection of heat-inactivated cells of the pathogen, but the actual level of protection was not examined . Becker and Fujihara reported that rainbow trout injected with heat-killed cells produced an agglutinating titer of 1:5120, and that 60-70% of the trout later survived an injection of 106 live F. columnare cells . Tilapia (Oreochromis niloticus (L.)) could mount a significant humoral response in plasma and cutaneous mucus to F. columnare after intraperitoneal immunization with formalin-killed sonicated cells in Freund’s complete adjuvant . Protection levels were not investigated in the latter study. Protection was obtained after oral immunization with heat-killed or formalin-killed cells of F. columnare in the fish feed of three-month-old coho salmon (Oncorhynchus kisutch) [22, 100]. However, the protection as observed in these studies did not go hand-in-hand with high agglutinin titers. Bath-immunization with a bacterin was shown to protect carp against experimental challenge but antibodies against F. columnare were not detected in sera from immunized fish . Song’s work with bacterins demonstrated unequivocally that immersion vaccination could result in high levels of protection, but field test results were inconsistent . Song was able to demonstrate that there was cross-protection between isolates and that there may be a common protective antigen among the strains tested . Polyvalent vaccines have also been tested. Using intraperitoneal injections of a combination of formalin-killed F. columnare, Aeromonas salmonicida and Yersinia ruckeri antigens, interference from A. salmonicida antigen was shown to suppress responses to two other antigens . Commercially available oral and bathing vaccines have been successfully tested in largemouth bass (Micropterus salmoides) fry and salmon, respectively [95, 103]. Vaccination trials are further elaborated on below.
4. Importance of environmental factors
Karvonen et al. described the effect of global warming on the prevalence of different fish parasites and bacteria . F. columnare could be one of the many taking advantage of this phenomenon. Indeed, transmission of columnaris disease is more efficient in higher temperatures [30, 105]. Holt et al. found that when steelhead trout (Salmo gairdneri) or coho salmon (0. kisutch) experimentally infected with F. columnare were held in water at 12 to 20°C, mortality increased with temperature . As stated above, adhesion to gill tissue of a highly virulent F. columnare strain is enhanced at increased temperature  and chondroitin AC lyase activity of this pathogen increases along with the temperature . The influence of rearing density and water temperature in rainbow trout was studied by Suomalaien et al. . Normal rearing densities with high temperatures (23°C) proved to increase both transmission rate of columnaris disease and mortality in the fish. Normal densities at low temperatures (18°C) did not affect mortality, but increased the transmission rate of columnaris disease .
Columnaris disease is furthermore influenced by water quality. Decostere et al. observed significantly higher bacterial titers on the gills when organic matter or nitrite were added to an organ bath when performing ex vivo trials with F. columnare. They discussed that organic matter could concentrate nutrients to feed the bacteria and that degrading enzymes could be kept in close contact with the host tissue. Bacterial titers were furthermore markedly lower in gills placed in an organ bath with distilled water with or without 0.03% NaCl compared to the titers of gills suspended in Ringer solution or in formulated water containing divalent ions (magnesium and calcium) . Morris et al. noted that the survival of the fish exposed to F. columnare significantly increased as unionized ammonia concentrations increased . These results suggest that complex interactions can complicate prediction of the responses of fish to concurrent chemical stressors and bacterial pathogens . Bandilla et al. described that co-infections of ectoparasites with F. columnare increased the susceptibility of rainbow trout to the bacterial pathogen . Compared with single infections, the mortality was significantly higher and the onset of disease condition occurred earlier in fish which were concomitantly infected by the parasite Argulus coregoni and F. columnare.
5. Experimental trials/pathogenicity tests
A reliable and reproducible experimental infection model is crucial for studying bacterium-host interactions and evaluating the efficacy of both curative and preventive measures. Most researchers recognize, in experimental challenges with F. columnare, the fine and delicate balance and complex interplay between the bacterial cells, fish and environment in the successful reproduction of columnaris disease.
Hitherto, columnaris disease has been reproduced experimentally in black mollies (Poecilia sphenops) , channel catfish [43, 44, 110, 111], eel , golden shiner (Notemigonus crysoleucas) , koi carp , rainbow trout [49, 65, 105, 113], tilapia  and zebrafish [43, 44].
In the various experimental infection trials, several ways to both cultivate and harvest the bacterial cells for inoculation were adopted. Different media were used to cultivate the bacteria, including Hsu-Shotts or modified Hsu-Shotts medium [79, 114], Ordal’s medium , Anacker and Ordal’s medium also referred to as “Cytophaga medium” , F. columnare growth medium broth  and Shieh or modified Shieh medium [12, 23, 43–45, 105]. The bacteria were mostly grown on a shaker [43–45, 75, 109–111]. Temperatures and incubation times of the bacteria varied from 21°C to up to 30°C and from 24 to 48 h, respectively.
To reproduce columnaris disease experimentally, largely two inoculation routes were adopted, viz. bath (immersion) and injection. With regard to immersion challenges, mostly full-grown broth cultures were added to the water with water temperatures of the immersion water varying from 18°C to 30°C [12, 23, 42–44, 111]. Only in a minority of cases were the bacterial cells first harvested through centrifugation and consequently resuspended before being added to the inoculation bath [33, 45, 114]. Times during which the fish were bath exposed varied from 15 min [13, 72, 105] over 30 min [12, 23, 43–45, 109] to 45 min , one hour [79, 94] and longer [110, 111]. When the bacterial cells were administered through injection, then they were retrieved from centrifuged broth cultures following discard of the supernatant  or immediately taken from a full-grown broth culture without primary centrifugation [13, 58, 94]. A third inoculation route consisted of adding dead fish that were first injected intraperitoneally with a virulent F. columnare strain, to the aquarium water in which fish were immersed . In some researches, the successful reproduction of columnaris disease depended on or the severity of the elicited disease increased by abrading the skin or gills of the fish [45, 94, 105] whereas in another study, clipping the fin had no effect on success after immersion challenge with F. columnare.
The infection route, which determines the way the bacterial cells are to be grown/collected, also defines the disease producing capacity of the adopted strains and the disease picture they elicit. Indeed, especially for low virulent strains, higher morbidity and mortality rates were noted after injection of fish as compared to inoculation through immersion . Pacha and Ordal reported a similar finding . They stated that contact with highly virulent strains induced infection and disease contraction more than intramuscular injection, whereas injection of low virulent bacteria more readily induced infection and disease contraction than did contact .
Environmental conditions can also highly influence morbidity and mortality rates during a challenge trial. To illustrate the dramatic effects of water temperature on the level of mortalities, the investigation of Holt et al. is particularly relevant . This team challenged steelhead trout (Oncorhynchus mykiss), Chinook salmon (O. tshawytscha) and coho salmon with F. columnare, via the water-borne route. At a water temperature of 9.4°C, there were no mortalities attributable to F. columnare. By increasing the temperature to 12.2°C, mortality became as high as 4-20%; at 20.5°C, all the steelhead trout and coho salmon and 70% of the Chinook salmon died . High (23°C) rearing temperatures also increased mortality significantly in rainbow trout compared to lower (18°C) temperatures . Fish density at fish farms is also a key player in influencing mortality in an outbreak of columnaris disease as mortality rates started earlier and remained higher when fish were stocked at high densities . Water flow is another important factor since it acts as a determining factor with regard to the contact time between the possibly present bacteria and the host tissue. High mortality rates were observed in elvers kept in standing water, while in aquaria with running water, mortality in elvers was reduced by half .
6.1 Preventive measures
Management plays a key role in the prevention of the disease. Cunningham et al. showed that some commonly recorded production variables (feed consumption, pond depth, ammonia levels and stocking events) were associated with columnaris disease outbreaks and, if monitored, could help identify “at risk” ponds prior to disease outbreaks . Suomalainen et al. pointed out that reduction of fish density could be used in the prevention of columnaris disease especially if water temperature is high . As lower rearing density can also decrease the transmission of ectoparasites and penetrating endoparasites, it could be an efficient tool in ecological disease management as a whole . High nitrite levels and organic load can stimulate the adherence capacity of F. columnare, and therefore it is important to control these parameters as well. Furthermore, water treatment could aid in averting a bacterial outbreak. Conrad et al. reported that ozone treatment of water significantly reduced the numbers of added F. columnare, which could be a practical method of prevention . Salt and acidic bath treatments could be used to disinfect water contaminated by F. columnare. An in vivo immersion challenge of F. columnare in channel catfish and goldfish (Carassius auratus L.) revealed decreasing mortality as salinity goes up, with significantly lower and no mortalities when salinity reaches values of 1.0‰ and between 3 and 9‰, respectively . If the fish can be adapted to salt levels of at least 1.0‰, this method could be used as a possible preventive measure in columnaris disease. Shoemaker et al. suggested that in the absence of natural food, juvenile channel catfish should be fed at least once every other day to apparent satiation in order to maintain normal physiological function and improve resistance to F. columnare, since deprivation reduced innate resistance of catfish to columnaris disease .
Besides optimizing and adjusting management practices, chemical agents can also be adopted as a preventive approach. Davis concluded that the development or intensification of columnaris disease could be prevented by treating the fish for 20 min in a copper sulfate (CuSO4) bath at 37 mg/L (1:30 000) or by adding copper sulfate to pond water at 0.5 mg/L . Dipping the fish one at a time in a 1:2000 copper-sulfate for one to two minutes was also proven to be effective in the prevention of the disease. Rogers suggested the addition of potassium permanganate (KMnO4) to the water at 2 mg/L . Darwish et al. also confirmed the prophylactic value of KMnO4 at doses around 2 mg/L . Prophylactic treatment of channel catfish with 15 mg/L chloramine-T reduced fish mortality from a F. columnare infection from 84–100% to 6–14% . Thomas-Jinu and Goodwin demonstrated the efficacy of prophylactically given oxytetracycline against mortality in channel catfish and also reported zero mortality for the combination of sulphadimethoxine and ormetoprim in feed prior to bacterial challenge with four highly virulent strains of F. columnare.
Another method to prevent columnaris disease is through vaccination. Although vaccination trials have not always been successful, success rates have increased as knowledge on fish immunity and its role in the defense against bacterial diseases continues to expand. Bath immunization with a bacterin was shown to protect carp against experimental challenge, but no agglutinin could be found in sera from immunized fish . Immersion of channel catfish in a bacterin, when performed each year, induced a significant decrease in mortality compared to unvaccinated fish . Fujihara and Nakatani obtained protection against columnaris disease in 3-month-old coho salmon by oral immunization with heat-killed cells of F. columnare incorporated into fish feed . Ransom proved that prolonged feeding (over three months) of formalin-killed bacteria provided high levels of protection . Ourth and Bachinski proposed that strains containing sialic acid could serve as potential vaccine strains for columnaris disease . As stated above, immunization with formalin-killed sonicated cells in Freund’s complete adjuvant injected intraperitoneally in tilapia resulted in a significant systemic humoral response within two weeks and antibody levels almost tripled following secondary immunization . At 10 weeks postimmunization, the mean antibody titer remained significantly elevated. Antibodies were also observed in cutaneous mucus of these fish at six and eight weeks postimmunization . An attenuated immersion vaccine currently is registered for the use in channel catfish in the USA . Fry between 10 to 48 days post hatch that were vaccinated through immersion achieved a relative percent survival (RPS) between 57 and 94% following F. columnare challenge. This vaccine was also proven to be efficient in largemouth bass fry resulting in RPS values between 74 and 94%, depending on the vaccine dose . Bebak et al. tested a commercial oral vaccine in largemouth bass fry . Vaccinated fish had a 43% lower risk of death by F. columnare during the field trial . An immersion vaccine consisting of a bacterin of F. columnare was also brought to the market in the USA as an aid in the prevention of columnaris disease in healthy salmonids of over three grams .
Probiotics appear to be a promising way in the prevention of different bacterial diseases in aquaculture . Boutin et al. isolated different strains of commensal bacteria from the skin mucus of unstressed brook charr (Salvelinus fontinalis) which in vitro revealed antagonistic effects against F. columnare. The strains were mixed and used to treat columnaris disease in an in vivo experiment. This resulted in a significant decrease of mortality indicating the potential use of these probiotic candidates in the efficient and durable management of columnaris disease. The immunostimulants β-glucan and β-hydroxy-β-methylbutyrate raised the levels of immune function parameters, but did not improve survival in challenge trials with F. columnare at any concentration of the stimulants used . The research group of Sink et al. demonstrated that mortality rates in golden shiners fed high-fat diets with a dairy-yeast prebiotic were significantly lower after a challenge trial with F. columnare.
Recent studies have demonstrated genetic variation in resistance towards F. columnare[73, 78, 124]. Arias et al. presented experimental data on the susceptibility to columnaris disease of hybrid catfish (female channel catfish × male blue catfish (I. furcatus)) (C×B) . Under experimental conditions, C×B hybrids were significantly more resistant to columnaris disease caused by a highly virulent strain of F. columnare belonging to genomovar II compared to channel catfish and blue catfish. Beck et al. also found one of the two investigated catfish families to be completely resistant towards F. columnare resulting in no mortality after inoculation with the bacteria . These F. columnare resistant families could be of great financial importance in the catfish industry. Interestingly, LaFrentz et al. found that the two families that exhibited the highest CPM after F. columnare challenges, had the lowest CPM following E. ictaluri challenge, the latter being the most prevalent bacterium to cause disease and mortality in the catfish industry . Further research on larger numbers of families is needed to determine whether there is any genetic correlation between resistance to E. ictaluri and resistance to F. columnare, the two leading bacterial diseases in the catfish industry .
6.2 Curative approach
Treatment of columnaris disease using antimicrobial agents has known different success rates. External treatments are possible only in early stages of the disease, when the infection is still superficial . Drugs which have been used effectively in bath therapies are chloramphenicol , nifurpirinol [127, 128], nifurprazine [129, 130] and oxolinic acid [131, 132]. If the disease is in an advanced stage and/or signs of septicaemia are observed, it is necessary to administer antimicrobials in the feed. Oxytetracycline given orally for up to 10 days proved effective in early as well as advanced outbreaks of columnaris disease in Pacific salmon (Salmo salar) [6, 133]. Lack of success of orally administered oxytetracycline has also been reported . Sulfonamides, such as sulfamerazine and sulfamethazine, can be used orally but would be less effective than other drugs [6, 135]. Nitrofuran can also be administered orally for 3 to 5 days [6, 96, 130]. Gaunt et al. demonstrated the efficacy of florfenicol in the feed against columnaris disease in channel catfish . Darwish et al. also illustrated the clear benefit of florfenicol against a mixed infection of A. hydrophila and F. columnare in Sunshine bass (hybrid striped bass, Morone chrysops female × Morone saxatilis male) . The excessive use of antimicrobial agents to withstand F. columnare has its negative attributes though . These include possible allergic reactions elicited in the user after food contact . Potential impacts on human health resulting from the emergence of drug-resistant bacteria and the associated risk of transfer of these resistant traits to the environment and human-associated bacteria are also a major concern . Declercq et al. demonstrated the in vitro multiple resistance of F. columnare strains originating from ornamental fish toward several clinically important antibiotics, such as quinolones and tetracyclines . The results obtained in this study appeal for less prudent use of antimicrobials especially in the ornamental fish industry and therefore urges to limit their use and to focus on the development of alternative curative and preventive measures against columnaris disease.
Besides resorting to antimicrobial agents, chemicals have also been adopted in the curative treatment of columnaris disease. In a study by Thomas-Jinu and Goodwin, the herbicide Diquat® (Zeneca Agricultural Products, Wilmington, DE, USA) was shown to significantly reduce channel catfish mortalities to zero percent after challenge with F. columnare. The herbicide has also proven to be effective in the treatment of columnaris disease in salmonids [6, 139]. Copper sulfate  and potassium permanganate  are among the older chemicals used for treatment and prevention of columnaris disease in pond fishes. The organic load in water affects the efficacy of potassium permanganate, but methods are available to estimate that organic load and compensate by adjusting the level of the chemical . Darwish et al. suggested that copper sulfate has clear therapeutic value against F. columnare infections in channel catfish when treated in an ultralow flow-through system during 4 h . Thomas-Jinu and Goodwin on the other hand proved the inefficacy of this same chemical against columnaris disease, which might be due to the advanced stage of the experimental infection at the time of treatment . An in vitro assay of commercial products containing peracetic acid was proven to be effective against F. columnare infection . Laanto et al. reported some Flavobacterium sp. phage lysates to inhibit growth or lyse the bacterial cultures . The authors recommended that the causative agent of this strong inhibition or lysis should be studied further for the possibility of developing antimicrobial agents. Prasad et al. also described the successful use of F. columnare phage FCP1 to combat columnaris disease in walking catfish (Claries batrachus) . Phage treatment led to disappearance of gross symptoms, resulted in a negative bacteriological test, a detectable phage, and 100% survival in experimentally infected C. batrachus. The result of this study opens new perspectives for the treatment of columnaris disease elicited by antimicrobial resistant F. columnare strains .
Despite the worldwide importance of columnaris disease and the multitude of studies focusing on its causative agent, only snippets of the bacterium-host interactions have hitherto been exposed. This results in major knowledge gaps still prevailing on amongst others how the pathogen is able to establish and maintain a grip on the skin and gill tissue and elicit disease and mortality. Methods for rapid identification of the bacterium in the environment, host tissue or in culture have indeed evolved rapidly and some techniques permit to trace even a few bacterial cells. However, the rationale for the difference in virulence among F. columnare strains remains to be unraveled. Full genome sequencing of the reference strain has been an important step towards gaining insight into the pathogenesis of columnaris disease. Efficient molecular manipulation systems could be of great importance to further explore this domain. Genetic knockouts could in effect illuminate virulence factors involved in the pathogenesis of this disease. Although various models to experimentally induce columnaris disease are available, there is a lack of a standardized experimental model hampering the comparison of retrieved data in between research groups and the drawing of consistent conclusions. Consistency in the cultivation protocol of the bacteria in terms of incubation temperature, infection dose, exposure time and infection route is desirable. Extrapolation of a standardized model from one to another fish species is not possible rendering it necessary to set-up an experimental model for each fish sensitive species. Besides further elucidating the pathogenesis of columnaris disease, the development of efficient curative and effective preventive measures also needs to continue. With the upcoming antimicrobial resistance, focus should be laid on the search for preventive measures. Management plans trying to optimize or adjust fish densities and control water quality parameters are a first critical step in controlling columnaris disease. Vaccines, chemotherapeutics and probiotics could possibly have a promising future in the prevention and control of the disease as some studies have already demonstrated. Another interesting envisaged method to mitigate columnaris disease is the creation of a fish breeding program introducing F. columnare resistant fish. Data generated through pathogenesis studies will allow for the further development and optimization of cost-effective and environmentally friendly prevention methods of columnaris disease in the various susceptible fish species.
The authors thank Prof. Paul Simoens of the Department of Morphology of the Faculty of Veterinary Medicine at Ghent University for critically reviewing this manuscript. Prof. Koen Chiers of the Department Bacteriology, Pathology and Avian Diseases of the Faculty of Veterinary Medicine at Ghent University is gratefully acknowledged for his expertise and help with the interpretation of the fish pathology. Prof. Maria Cornelissen and Leen Pieters of the Department of Anatomy, Embryology, Histology and Medical Physics of the Faculty of Medicine and Health Sciences at Ghent University are accredited for their cooperation. Furthermore, we also give thanks to Lobke De Bels, Jurgen De Craene, Bart De Pauw and Patrick Vervaet for their technical assistance. The financial support by the Special Research Grant (Bijzonder Onderzoeksfonds, BOF, grant number 01Z06210) of Ghent University, Belgium is gratefully acknowledged.
- Bernardet JF, Bowman JP: The genus Flavobacterium. The Prokaryotes: A Handbook on the Biology of Bacteria: Volume 7: Proteobacteria: Delta and Epsilon Subclasses. Deeply Rooting Bacteria. Edited by: Dworkin M, Falkow S. 2006, New York: Springer Science+Business Media, LLC, 481-531.Google Scholar
- Bernardet JF, Nakagawa Y, Holmes B: Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol. 2002, 52: 1049-1070. 10.1099/ijs.0.02136-0.PubMedGoogle Scholar
- Bernardet JF, Segers P, Vancanneyt M, Berthe M, Kersters K, Vandamme P: Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Evol Bacteriol. 1996, 46: 128-148. 10.1099/00207713-46-1-128.View ArticleGoogle Scholar
- Davis HS: A new bacterial disease in freshwater fishes. United States Bureau of Fisheries Bulletin. 1922, 38: 37-63.Google Scholar
- Ordal EJ, Rucker RR: Pathogenic myxobacteria. PSEBM. 1944, 56: 15-18.Google Scholar
- Bullock GL, Hsu TC, Shotts EB: Columnaris disease of fishes. USFWS Fish Disease Leaflet. 1986, 1986: 1-9.Google Scholar
- Garnjobst L: Cytophaga columnaris (Davis) in pure culture: a myxobacterium pathogenic to fish. J Bacteriol. 1945, 49: 113-128.PubMed CentralPubMedGoogle Scholar
- Bernardet J-F, Grimont PAD: Deoxyribonucleic acid relatedness and phenotypic characterization of Flexibacter columnaris sp. nov., norn. rev., Flexibacter psychrophilus sp. nov., nom. rev., and Flexibacter maritimus Wakabayashi, Hikida, and Masumura 1986. IJSB. 1989, 39: 346-354.Google Scholar
- Triyanto , Wakabayashi H: Genotypic diversity of strains of Flavobacterium columnare from diseased fishes. Fish Pathol. 1999, 34: 65-71. 10.3147/jsfp.34.65.View ArticleGoogle Scholar
- Ourth DD, Bachinski LM: Bacterial sialic acid modulates activation of the alternative complement pathway of channel catfish (Ictalurus punctatus). Dev Comp Immunol. 1987, 11: 551-564. 10.1016/0145-305X(87)90044-9.PubMedView ArticleGoogle Scholar
- Decostere A, Haesebrouck F, Van Driessche E, Charlier G, Ducatelle R: Characterization of the adhesion of Flavobacterium columnare (Flexibacter columnaris) to gill tissue. J Fish Dis. 1999, 22: 465-474. 10.1046/j.1365-2761.1999.00198.x.View ArticleGoogle Scholar
- Decostere A, Haesebrouck F, Devriese LA: Characterization of four Flavobacterium columnare (Flexibacter columnaris) strains isolated from tropical fish. Vet Microbiol. 1998, 62: 35-45. 10.1016/S0378-1135(98)00196-5.PubMedView ArticleGoogle Scholar
- Figueiredo HCP, Klesius PH, Arias CR, Evans J, Shoemaker CA, Pereira DJ, Peixoto MTD: Isolation and characterization of strains of Flavobacterium columnare from Brazil. J Fish Dis. 2005, 28: 199-204. 10.1111/j.1365-2761.2005.00616.x.PubMedView ArticleGoogle Scholar
- Morley NJ, Lewis JW: Consequences of an outbreak of columnaris disease (Flavobacterium columnare) to the helminth fauna of perch (Perca fluviatilis) in the Queen Mary reservoir, south-east England. J Helminthol. 2010, 84: 186-192. 10.1017/S0022149X09990459.PubMedView ArticleGoogle Scholar
- Řehulka J, Minařík B: Blood parameters in brook trout Salvelinus fontinalis (Mitchill, 1815), affected by columnaris disease. Aquac Res. 2007, 38: 1182-1197. 10.1111/j.1365-2109.2007.01786.x.View ArticleGoogle Scholar
- Soto E, Mauel MJ, Karsi A, Lawrence ML: Genetic and virulence characterization of Flavobacterium columnare from channel catfish (Ictalurus punctatus). J Appl Microbiol. 2008, 104: 1302-1310. 10.1111/j.1365-2672.2007.03632.x.PubMedView ArticleGoogle Scholar
- Suomalainen L-R, Bandilla M, Valtonen ET: Immunostimulants in prevention of columnaris disease of rainbow trout, Oncorhynchus mykiss (Walbaum). J Fish Dis. 2009, 32: 723-726. 10.1111/j.1365-2761.2009.01026.x.PubMedView ArticleGoogle Scholar
- Hawke JP, Thune RL: Systemic isolation and antimicrobial susceptibility of Cytophaga columnaris from commercially reared channel catfish. J Aquat Anim Health. 1992, 4: 109-113. 10.1577/1548-8667(1992)004<0109:SIAASO>2.3.CO;2.View ArticleGoogle Scholar
- Wagner BA, Wise DJ, Khoo LH, Terhune JS: The epidemiology of bacterial diseases in food-size channel catfish. J Aquat Anim Health. 2002, 14: 263-272. 10.1577/1548-8667(2002)014<0263:TEOBDI>2.0.CO;2.View ArticleGoogle Scholar
- Shoemaker CA, Klesius PH, Drennan JD, Evans J: Efficacy of a modified live Flavobacterium columnare vaccine in fish. Fish Shellfish Immun. 2011, 30: 304-308. 10.1016/j.fsi.2010.11.001.View ArticleGoogle Scholar
- Barker GA, Smith SN, Bromage NR: Effect of oxolinic acid on bacterial flora and hatching success rate of rainbow trout, Oncorhynchus mykiss, eggs. Aquaculture. 1990, 9: 205-222.View ArticleGoogle Scholar
- Fujihara MP, Nakatani RE: Antibody protection and immune responses of rainbow trout coho salmon to Chondrococcus columnaris. J Fish Res Bd Canada. 1971, 28: 1253-1258. 10.1139/f71-191.View ArticleGoogle Scholar
- Suomalainen L-R, Tiirola M, Valtonen ET: Treatment of columnaris disease of rainbow trout: low pH and salt as possible tools?. Dis Aquat Organ. 2005, 65: 115-120.PubMedView ArticleGoogle Scholar
- Kunttu HMT, Valtonen ET, Jokinen EI, Suomalainen L-R: Saprophytism of a fish pathogen as a transmission strategy. Epidemics. 2009, 1: 96-100. 10.1016/j.epidem.2009.04.003.PubMedView ArticleGoogle Scholar
- Fijan FJ: Antibiotic additives for isolation of Chondrococcus columnaris from fish. Appl Microbiol. 1968, 17: 333-334.Google Scholar
- Chowdhury MBR, Wakabayashi H: Survival of four major bacterial fish pathogens in different types of experimental water. Bangladesh J Microbiol. 1990, 7: 47-54.Google Scholar
- Ross AJ, Smith CA: Effect of temperature on survival of Aeromonas liquefaciens, Aeromonas salmonicida, Chondrococcus columnaris, and Pseudomonas fluorescens. Progr Fish Cult. 1974, 36: 51-52. 10.1577/1548-8659(1974)36[51:EOTOSO]2.0.CO;2.View ArticleGoogle Scholar
- Kunttu HMT, Sundberg L-R, Pulkkinen K, Valtonen ET: Environment may be the source of Flavobacterium columnare outbreaks at fish farms. Environ Microbiol Rep. 2012, 4: 398-402. 10.1111/j.1758-2229.2012.00342.x.PubMedView ArticleGoogle Scholar
- Wakabayashi H: Columnaris disease. Bacterial diseases of fish. Edited by: Inglis V, Roberts RJ, Bromage NR. 1993, Oxford, England: Blackwell Scientific Publications, 23-39.Google Scholar
- Pulkkinen K, Suomalainen L-R, Read AF, Ebert D, Rintamäki P, Valtonen ET: Intensive fish farming and the evolution of pathogen virulence: the case of columnaris disease in Finland. Proc Biol Sci. 2010, 277: 593-600. 10.1098/rspb.2009.1659.PubMed CentralPubMedView ArticleGoogle Scholar
- Rucker RR, Earp BJ, Ordal EJ: Infectious diseases of Pacific salmon. Trans Am Fish Soc. 1953, 83: 297-312.View ArticleGoogle Scholar
- Pacha RE, Ordal EJ: Histopathology of experimental columnaris disease in young salmon. J Comp Path. 1967, 77: 419-423. 10.1016/0021-9975(67)90027-8.PubMedView ArticleGoogle Scholar
- Foscarini R: Induction and development of bacterial gill disease in the eel (Anguilla japonica) experimentally infected with Flexibacter columnaris: pathological changes in the gill vascular structure and in cardiac performance. Aquaculture. 1989, 78: 1-20. 10.1016/0044-8486(89)90002-1.View ArticleGoogle Scholar
- Pacha RE, Ordal EJ: Myxobacterial diseases of salmonids. A symposium on diseases of fishes and shellfishes. Edited by: American Fisheries Society special publication, Snieszko SF. 1970, 243-257. 5Google Scholar
- Decostere A: Flavobacterium columnare infections in fish: the agent and its adhesion to the gill tissue. Verhandelingen - KAGB. 2002, 64: 421-430.Google Scholar
- Morrison C, Cornick J, Shum G, Zwicker B: Microbiology and histopathology of “saddle-back” disease of underyearling Atlantic salmon, Salmo salar. J Bacteriol. 1981, 78: 225-230.Google Scholar
- Decostere A, Haesebrouck F: Outbreak of columnaris disease in tropical aquarium fish. Vet Rec. 1999, 144: 23-24. 10.1136/vr.144.1.23.PubMedView ArticleGoogle Scholar
- Ferguson HW: Systemic Pathology of Fish. 2006, London: Scottian Press, 2Google Scholar
- Michel C, Messiaen S, Bernardet J-F: Muscle infections in imported neon tetra, Paracheirodon innesi Myers: limited occurrence of microsporidia and predominance of severe forms of columnaris disease caused by an Asian genomovar of Flavobacterium columnare. J Fish Dis. 2002, 25: 253-263. 10.1046/j.1365-2761.2002.00364.x.View ArticleGoogle Scholar
- Dalsgaard I: Virulence mechanisms in Cytophaga psychrophila and other Cytophaga-like bacteria pathogenic for fish. Ann Rev Fish Dis. 1993, 3: 127-144.View ArticleGoogle Scholar
- Decostere A, Haesebrouck F, Devriese L: Development of a medium for the selective isolation of Flavobacterium columnare from diseased fish. J Clin Microbiol. 1997, 35: 322-324.PubMed CentralPubMedGoogle Scholar
- Decostere A, Haesebrouck F, Charlier G, Ducatelle R: The association of Flavobacterium columnare strains of high and low virulence with gill tissue of black mollies (Poecilia sphenops). Vet Microbiol. 1999, 67: 287-298. 10.1016/S0378-1135(99)00050-4.PubMedView ArticleGoogle Scholar
- Olivares-Fuster O, Bullard SA, McElwain A, Llosa MJ, Arias CA: Adhesion dynamics of Flavobacterium columnare to channel catfish Ictalurus punctatus and zebrafish Danio rerio after immersion challenge. Dis Aquat Organ. 2011, 96: 221-227. 10.3354/dao02371.PubMedView ArticleGoogle Scholar
- Bullard SA, McElwain A, Arias CR: Scanning electron microscopy of “saddleback” lesions associated with experimental infections of Flavobacterium columnare in channel catfish, Ictalurus punctatus (Siluriformes: Ictaluridae), and zebrafish, Danio rerio (Cypriniformes: Cyprinidae). J World Aquacult Soc. 2011, 42: 906-913. 10.1111/j.1749-7345.2011.00527.x.View ArticleGoogle Scholar
- Tripathi NK, Latimer KS, Gregory CR, Ritchie BW, Wooley RE, Walker RL: Development and evaluation of an experimental model of cutaneous columnaris disease in koi Cyprinus carpio. J Vet Diagn Invest. 2005, 17: 45-54. 10.1177/104063870501700109.PubMedView ArticleGoogle Scholar
- Shieh HS: Studies on the nutrition of a fish pathogen, Flexibacter columnaris. Microbios Letters. 1980, 13: 129-133.Google Scholar
- Holt RA: Cytophaga psychrophila, the causative agent of bacterial cold-water disease in salmonid fish. 1988, Corvallis, OR: Oregon State UniversityGoogle Scholar
- Bader JA, Shoemaker CA, Klesius PH: Production, characterization and evaluation of virulence of an adhesion defective mutant of Flavobacterium columnare produced by β-lactam selection. Lett Appl Microbiol. 2005, 40: 123-127. 10.1111/j.1472-765X.2004.01641.x.PubMedView ArticleGoogle Scholar
- Kunttu HMT, Jokinen EI, Valtonen ET, Sundberg L-R: Virulent and nonvirulent Flavobacterium columnare colony morphologies: characterization of chondroitin AC lyase activity and adhesion to polystyrene. J Appl Microbiol. 2011, 111: 1319-1326. 10.1111/j.1365-2672.2011.05149.x.PubMedView ArticleGoogle Scholar
- Bernardet JF: “Flexibacter columnaris”: first description in France and comparison with bacterial strains from other origins. Dis Aquat Organ. 1989, 6: 37-44.View ArticleGoogle Scholar
- Olivares-Fuster O, Baker JL, Terhune JS, Shoemaker CA, Klesius PH, Arias CR: Host-specific association between Flavobacterium columnare genomovars and fish species. Syst Appl Microbiol. 2007, 30: 624-633. 10.1016/j.syapm.2007.07.003.PubMedView ArticleGoogle Scholar
- Darwish AM, Ismaiel AA, Newton JC, Tang J: Identification of Flavobacterium columnare by a species-specific polymerase chain reaction and renaming of ATCC43622 strain to Flavobacterium johnsoniae. Mol Cell Probes. 2004, 18: 421-427. 10.1016/j.mcp.2004.07.002.PubMedView ArticleGoogle Scholar
- Welker TL, Shoemaker CA, Arias CR, Klesius PH: Transmission and detection of Flavobacterium columnare in channel catfish Ictalurus punctatus. Dis Aquat Organ. 2005, 63: 129-138.PubMedView ArticleGoogle Scholar
- Shoemaker CA, Shelby RA, Klesius PH: Development of an indirect ELISA to detect humoral response to Flavobacterium columnare infection of channel catfish, Ictalurus punctatus. J Appl Aquaculture. 2003, 14: 43-52. 10.1300/J028v14n01_03.View ArticleGoogle Scholar
- Panangala VS, Shelby RA, Shoemaker CA, Klesius PH, Mitra A, Morrison EE: Immunofluorescent test for simultaneous detection of Edwardsiella ictaluri and Flavobacterium columnare. Dis Aquat Organ. 2006, 68: 197-207.View ArticleGoogle Scholar
- Speare DJ, Markham RJ, Despres B, Whitman K, MacNair N: Examination of gill from salmonids with bacterial gill disease using monoclonal antibody probes for Flavobacterium branchiophilum and Cytophaga columnaris. J Vet Diagn Invest. 1995, 7: 500-505. 10.1177/104063879500700413.PubMedView ArticleGoogle Scholar
- Yeh H-Y, Shoemaker CA, Klesius PH: Sensitive and rapid detection of Flavobacterium columnare in channel catfish Ictalurus punctatus by a loop-mediated isothermal amplification method. J Appl Microbiol. 2006, 100: 919-925. 10.1111/j.1365-2672.2006.02853.x.PubMedView ArticleGoogle Scholar
- Panangala VS, Shoemaker CA, Klesius PH: TaqMan real-time polymerase chain reaction assay for rapid detection of Flavobacterium columnare. Aquac Res. 2007, 38: 508-517. 10.1111/j.1365-2109.2007.01695.x.View ArticleGoogle Scholar
- Suzuki M, Rappe MS, Giovanni SJ: Kinetic bias in estimates of coastal picoplankton community structure obtained by measurements of small-subunit rRNA gene PCR amplicon length heterogeneity. Appl Environ Microbiol. 1998, 64: 4522-4529.PubMed CentralPubMedGoogle Scholar
- Suomalainen L-R, Tiirola MA, Valtonen ET: Effect of Pseudomonas sp. MT5 baths on Flavobacterium columnare infection in rainbow trout and on microbial diversity on fish skin and gills. Dis Aquat Organ. 2005, 63: 61-68.PubMedView ArticleGoogle Scholar
- Decostere A, Haesebrouck F, Turnbull JF, Charlier G: Influence of water quality and temperature on adhesion of high and low virulence Flavobacterium columnare strains to isolated gill arches. J Fish Dis. 1999, 22: 1-11. 10.1046/j.1365-2761.1999.00132.x.View ArticleGoogle Scholar
- Klesius PH, Pridgeon JW, Aksoy M: Chemotactic factors of Flavobacterium columnare to skin mucus of healthy channel catfish (Ictalurus punctatus). FEMS Microbiol Lett. 2010, 310: 145-151. 10.1111/j.1574-6968.2010.02060.x.PubMedView ArticleGoogle Scholar
- Klesius PH, Shoemaker CA, Evans JJ: Flavobacterium columnare chemotaxis to channel catfish mucus. FEMS Microbiol Lett. 2008, 288: 216-220. 10.1111/j.1574-6968.2008.01348.x.PubMedView ArticleGoogle Scholar
- Kunttu H: Characterizing the bacterial fish pathogen Flavobacterium columnare and some factors affecting its pathogenicity. Jyväskyla Stud Biol Environ Sci. 2010, 206: 1-69.Google Scholar
- Suomalainen L-R, Tiirola M, Valtonen ET: Chondroitin AC lyase activity is related to virulence of fish pathogenic Flavobacterium columnare. J Fish Dis. 2006, 29: 757-763. 10.1111/j.1365-2761.2006.00771.x.PubMedView ArticleGoogle Scholar
- Li N, Zhang LQ, Zhang J, Liu ZX, Huang B, Zhang SH, Nie P: Type I restriction-modification system and its resistance in electroporation efficiency in Flavobacterium columnare. Vet Microbiol. 2012, 160: 61-68. 10.1016/j.vetmic.2012.04.045.PubMedView ArticleGoogle Scholar
- Staroscik AM, Nelson DR: The influence of salmon surface mucus on the growth of Flavobacterium columnare. J Fish Dis. 2008, 31: 59-69.PubMedView ArticleGoogle Scholar
- Zhang J, Zou H, Wang LF, Li N, Wang GT, Huang B, Nie P: Construction of two selective markers for integrative/conjugative plasmids in Flavobacterium columnare. Chin J Oceanol Limnol. 2012, 30: 269-278. 10.1007/s00343-012-1077-z.View ArticleGoogle Scholar
- Tekedar HC, Karsi A, Gillaspy AF, Dyer DW, Benton NR, Zaitshik J, Vamenta S, Banes MM, Gülsoy N, Aboko-Cole M, Waldbieser GC, Lawrence ML: Genome sequence of the fish pathogen Flavobacterium columnare ATCC 49512. J Bacteriol. 2012, 194: 2763-2764. 10.1128/JB.00281-12.PubMed CentralPubMedView ArticleGoogle Scholar
- Pate JL, Ordal EJ: The fine structure of Chondrococcus columnaris. III. The surface layers of Chondrococcus columnaris. J Cell Biol. 1967, 35: 37-51. 10.1083/jcb.35.1.37.PubMed CentralPubMedView ArticleGoogle Scholar
- LaFrentz BR, Klesius PH: Development of a culture independent method to characterize the chemotactic response of Flavobacterium columnare to fish mucus. J Microbiol Meth. 2009, 77: 37-40. 10.1016/j.mimet.2008.12.011.View ArticleGoogle Scholar
- Shoemaker CA, Olivares-Fuster O, Arias CR, Klesius PH: Flavobacterium columnare genomovar influences mortality in channel catfish (Ictalurus punctatus). Vet Microbiol. 2008, 127: 353-359. 10.1016/j.vetmic.2007.09.003.PubMedView ArticleGoogle Scholar
- LaFrentz BR, LaPatra SE, Shoemaker CA, Klesius PH: Reproducible challenge model to investigate the virulence of Flavobacterium columnare genomovars in rainbow trout Oncorhynchus mykiss. Dis Aquat Organ. 2012, 101: 115-122. 10.3354/dao02522.PubMedView ArticleGoogle Scholar
- Kunttu HMT, Suomalainen L-R, Jokinen EI, Valtonen ET: Flavobacterium columnare colony types: connection to adhesion and virulence?. Microb Pathog. 2009, 46: 21-27. 10.1016/j.micpath.2008.10.001.PubMedView ArticleGoogle Scholar
- Arias CR, LaFrentz S, Cai W, Olivares-Fuster O: Adaptive response to starvation in the fish pathogen Flavobacterium columnare: cell viability and ultrastructural changes. BMC Microbiol. 2012, 12: 266-276. 10.1186/1471-2180-12-266.PubMed CentralPubMedView ArticleGoogle Scholar
- Sun F, Peatman E, Li C, Liu S, Jiang Y, Zhou Z, Liu Z: Transcriptomic signatures of attachment, NF-κB suppression and IFN stimulation in the catfish gill following columnaris bacterial infection. Dev Comp Immunol. 2012, 38: 169-180. 10.1016/j.dci.2012.05.006.PubMedView ArticleGoogle Scholar
- Tateno H, Yamaguchi T, Ogawa T, Muramoto K, Watanabe T, Kamiya H, Saneyoshi M: Immunohistochemical localization of rhamnose-binding lectins in the steelhead trout (Oncorhynchus mykiss). Dev Comp Immunol. 2002, 26: 543-550. 10.1016/S0145-305X(02)00007-1.PubMedView ArticleGoogle Scholar
- Beck BH, Farmer BD, Straus DL, Li C, Peatman E: Putative roles for a rhamnose binding lectin in Flavobacterium columnare pathogenesis in channel catfish Ictalurus punctatus. Fish Shellfish Immun. 2012, 33: 1008-1015.View ArticleGoogle Scholar
- Altinok I, Grizzle JM: Effects of low salinities on Flavobacterium columnare infection of euryhaline and freshwater stenohaline fish. J Fish Dis. 2001, 24: 361-367. 10.1046/j.1365-2761.2001.00306.x.View ArticleGoogle Scholar
- Decostere A, Henckaerts K, Ducatelle R, Haesebrouck F: An alternative model to study the association of rainbow trout (Oncorhynchus mykiss L.) pathogens with the gill tissue. Lab Anim. 2002, 36: 396-402. 10.1258/002367702320389053.PubMedView ArticleGoogle Scholar
- Ofek I, Hasty DL, Doyle RJ: Adhesins as Bacterial Cell Surface Structures: General Concepts of Structure, Biogenesis, and Regulation. Bacterial adhesion to animal cells and tissues. Edited by: ASM Press. 2003, Washington D.C.: ASM Press, 77-81.View ArticleGoogle Scholar
- Romero M, Avendaño-Herrera R, Magariños B, Cámara M, Otero A: Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritinum, a member of the Cytophaga-Flavobacterium_Bacteroides (CFB) group. FEMS Microbiol Lett. 2009, 304: 131-139.View ArticleGoogle Scholar
- Camilli A, Bassler BL: Bacterial small-molecule signaling pathways. Science. 2006, 311: 1113-1116. 10.1126/science.1121357.PubMed CentralPubMedView ArticleGoogle Scholar
- Wagner-Döbler I, Thiel V, Eberl L, Allgaier M, Bodor A, Meyer S, Ebner S, Henning A, Pukall R, Schulz S: Discovery of complex mixtures of novel long-chain quorum sensing signals in free-living and host-associated marine Alphaproteobacteria. Chem Bio Chem. 2005, 6: 2195-2206. 10.1002/cbic.200500189.PubMedView ArticleGoogle Scholar
- Griffin BR: Characteristics of a chondroitin AC lyase produced by Cytophaga columnaris. T Am Fish Soc. 1991, 120: 391-395. 10.1577/1548-8659(1991)120<0391:COACAL>2.3.CO;2.View ArticleGoogle Scholar
- Newton JC, Wood TM, Hartley MM: Isolation and partial characterization of extracellular proteases produced by isolates of Flavobacterium columnare derived from channel catfish. J Aquat Anim Health. 1997, 9: 75-85. 10.1577/1548-8667(1997)009<0075:IAPCOE>2.3.CO;2.View ArticleGoogle Scholar
- Anacker RL, Ordal EJ: Studies on the myxobacterium Chondrococcus columnaris. II. Bacteriocins. J Bacteriol. 1959, 78: 33-40.PubMedGoogle Scholar
- Tiirola M, Valtonen ET, Rintamaki-Kinnunen P, Kulomaa MS: Diagnosis of flavobacteriosis by direct amplification of rRNA genes. Dis Aquat Organ. 2002, 51: 93-100.PubMedView ArticleGoogle Scholar
- Zhang Y, Arias CR, Shoemaker CA, Klesius PH: Comparison of lipopolysaccharide and protein profiles between Flavobacterium columnare strains from different genomovars. J Fish Dis. 2006, 29: 657-663. 10.1111/j.1365-2761.2006.00760.x.PubMedView ArticleGoogle Scholar
- Aranishi F, Mano N, Nakane M, Hirose H: Epidermal response of the Japanese eel to environmental stress. Fish Physiol Biochem. 1998, 19: 197-203. 10.1023/A:1007746514851.View ArticleGoogle Scholar
- Do Vale A, Marques F, Silva MT: Apoptosis of sea bass (Dicentrarchus labrax L.) neutrophils and macrophages induced by experimental infection with Photobacterium damselae subsp. piscicida. Fish Shellfish Immun. 2003, 15: 129-144. 10.1016/S1050-4648(02)00144-4.View ArticleGoogle Scholar
- Ourth DD, Wilson EA: Bactericidal serum response of the channel catfish against Gram-negative bacteria. Dev Comp Immunol. 1982, 6: 579-583. 10.1016/S0145-305X(82)80044-X.PubMedView ArticleGoogle Scholar
- Ourth DD, Bachinski LM: Bactericidal response of channel catfish Ictalurus punctatus by the classical and alternative complement pathways against bacterial pathogens. J Appl Ichtyol. 1987, 3: 42-45. 10.1111/j.1439-0426.1987.tb00449.x.View ArticleGoogle Scholar
- Kuo S-C, Chung HY, Kou GH: Studies on artificial infection of the gliding bacteria in cultured fishes. Fish Pathol. 1981, 15: 309-314. 10.3147/jsfp.15.309.View ArticleGoogle Scholar
- Bebak J, Matthews M, Shoemaker C: Survival of vaccinated, feed-trained largemouth bass fry (Micropterus salmoides floridanus) during natural exposure to Flavobacterium columnare. Vaccine. 2009, 27: 4297-4301. 10.1016/j.vaccine.2009.05.026.PubMedView ArticleGoogle Scholar
- Becker CD, Fujihara MP: The bacterial pathogen, Flexibacter columnaris and its epizootiology among Columbia river fish. 1978, Washington, D.C.: American Fisheries SocietyGoogle Scholar
- Grabowski LD, LaPatra SE, Cain KD: Systemic and mucosal antibody response in tilapia, Oreochromis niloticus (L.), following immunization with Flavobacterium columnare. J Fish Dis. 2004, 27: 573-581. 10.1111/j.1365-2761.2004.00576.x.PubMedView ArticleGoogle Scholar
- Moore AA, Eimers ME, Cardella MA: Attempts to control Flexibacter columnaris epizootics in pond-reared channel catfish by vaccination. J Aquat Anim Health. 1990, 2: 109-111. 10.1577/1548-8667(1990)002<0109:ATCCEI>2.3.CO;2.View ArticleGoogle Scholar
- Schachte JHJ, Mora EC: Production of agglutinating antibodies in the channel catfish (Ictalurus punctatus) against Chondrococcus columnaris. J Fish Res Board Canada. 1973, 30: 116-118. 10.1139/f73-017.View ArticleGoogle Scholar
- Ransom DP: Immune responses of salmonids: (a) oral immunization against Flexibacter columnaris. (b) effects of combining antigens in parenterally administered polyvalent vaccines. 1975, Corvallis, OR: MS Thesis. Oregon State UniversityGoogle Scholar
- Liewes EW, Van Dam RH: Procedures and application of the in vitro fish leukocyte stimulation assay. Dev Comp Immunol. 1982, 2: 223-232.Google Scholar
- Song Y-L: Comparison of Flexibacter columnaris strains isolated from fish in North America and other areas of the Pacific Rim. PhD Thesis. 1986, Corvallis, OR: Oregon State UniversityGoogle Scholar
- AFS-FCS (2011):Guide to using drugs, biologics, and other chemicals in aquaculture. American fisheries society fish culture section.http://www.fws.gov/fisheries/aadap/AFS-FCS%20documents/GUIDE_FEB_2011.pdf,
- Karvonen A, Rintamäki P, Jokela J, Valtonen ET: Increasing water temperature and disease risks in aquatic systems: climate change increases the risk of some, but not all, diseases. Int J Parasitol. 2010, 40: 1483-1488. 10.1016/j.ijpara.2010.04.015.PubMedView ArticleGoogle Scholar
- Suomalainen L-R, Tiirola M, Valtonen ET: The influence of rearing conditions on Flavobacterium columnare infection of rainbow trout. J Fish Dis. 2005, 28: 271-277. 10.1111/j.1365-2761.2005.00631.x.PubMedView ArticleGoogle Scholar
- Holt RA, Sanders JE, Zinn JL, Fryer JL, Pilcher KS: Relation of water temperature to Flexibacter columnaris infection in steelhead trout (Salmo gairdneri) coho (Oncorhynchus kisutch), and chinook (0. tshawytscha) salmon. J Fish Res Board Can. 1975, 32: 1553-1559. 10.1139/f75-182.View ArticleGoogle Scholar
- Suomalainen L-R, Reunanen H, Ijäs R, Valtonen ET, Tiirola M: Freezing induces biased results in the molecular detection of Flavobacterium columnare. Appl Environ Microbiol. 2006, 72: 1702-1704. 10.1128/AEM.72.2.1702-1704.2006.PubMed CentralPubMedView ArticleGoogle Scholar
- Morris JM, Snyder-Conn E, Foott JS, Holt RA, Suedkamp MJ, Lease HM, Clearwater SJ, Meyer JS: Survival of lost river suckers (Deltistes luxatus) challenged with Flavobacterium columnare during exposure to sublethal ammonia concentrations at pH 9.5. Arch Environ Contam Toxicol. 2006, 50: 256-263. 10.1007/s00244-004-0194-x.PubMedView ArticleGoogle Scholar
- Bandilla M, Valtonen ET, Suomalainen L-R, Aphalo PJ, Hakalahti T: A link between ectoparasite infection and susceptibility to bacterial disease in rainbow trout. Int J Parasitol. 2006, 36: 987-991. 10.1016/j.ijpara.2006.05.001.PubMedView ArticleGoogle Scholar
- Darwish AM, Mitchell A, Straus DL: Evaluation of a 4-h static copper sulphate treatment against experimental infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus). Aquaculture Res. 2011, 43: 688-695.View ArticleGoogle Scholar
- Thomas-Jinu S, Goodwin AE: Acute columnaris infection in channel catfish, Ictalurus punctatus (Rafinesque): efficacy of practical treatments for warmwater aquaculture ponds. J Fish Dis. 2004, 27: 23-28. 10.1046/j.1365-2761.2003.00504.x.PubMedView ArticleGoogle Scholar
- Sink TD, Lochmann RT, Goodwin AE, Mareceaux E: Mortality rates in golden shiners fed high-fat diets with or without a dairy-yeast prebiotic before challenge with Flavobacterium columnare. N Am J Aquaculture. 2007, 69: 305-308. 10.1577/A06-078.1.View ArticleGoogle Scholar
- Kunttu HMT, Valtonen ET, Suomalainen L-R, Vielma J, Jokinen IE: The efficacy of two immunostimulants against Flavobacterium columnare infection in juvenile rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immun. 2009, 26: 850-857. 10.1016/j.fsi.2009.03.013.View ArticleGoogle Scholar
- Bader JA, Nusbaum KE, Shoemaker CA: Comparative challenge model of Flavobacterium columnare using abraded and unabraded channel catfish, Ictalurus punctatus (Rafinesque). J Fish Dis. 2003, 26: 461-467. 10.1046/j.1365-2761.2003.00479.x.PubMedView ArticleGoogle Scholar
- Darwish AM, Bebak JA, Schrader KK: Assessment of Aquaflor®, copper sulphate and potassium permanganate for control of Aeromonas hydrophila and Flavobacterium columnare infection in sunshine bass, Morone chrysops female x Morone saxatilis male. J Fish Dis. 2012, 35: 637-647. 10.1111/j.1365-2761.2012.01393.x.PubMedView ArticleGoogle Scholar
- Cunningham FL, Jack SW, Hardin D, Wills RW: Pond-Level risk factors associated with columnaris disease on Mississippi commercial catfish farms. J Aquat Anim Health. 2012, 24: 178-184. 10.1080/08997659.2012.675932.PubMedView ArticleGoogle Scholar
- Conrad JF, Holt RA, Kreps TD: Ozone disinfection of flowing water. Prog Fish-Cult. 1975, 37: 134-136. 10.1577/1548-8659(1975)37[134:ODOFW]2.0.CO;2.View ArticleGoogle Scholar
- Shoemaker CA, Klesius PH, Lim C, Yildirim M: Feed deprivation of channel catfish, Ictalurus punctatus (Rafinesque), influences organosomatic indices, chemical composition and susceptibility to Flavobacterium columnare. J Fish Dis. 2003, 26: 553-561. 10.1046/j.1365-2761.2003.00489.x.PubMedView ArticleGoogle Scholar
- Rogers WA: Principal diseases of catfish: how to identify and fight them. Fish Farming Ind. 1971, 2: 20-26.Google Scholar
- Darwish AM, Mitchell AJ, Straus DL: Evaluation of potassium permanganate against an experimental subacute infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus (Rafinesque). J Fish Dis. 2009, 32: 193-199. 10.1111/j.1365-2761.2008.01015.x.PubMedView ArticleGoogle Scholar
- Riley TA: Treatment of Flavobacterium columnare and toxicity of chloramine-T in channel catfish. 2000, Ictalurus punctatus. MS thesis: Auburn University, Auburn, AlabamaGoogle Scholar
- Merrifield DL, Dimitroglou A, Foey A, Davies SJ, Baker RTM, Bøgwald J, Castex M, Ringø E: The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture. 2010, 302: 1-18. 10.1016/j.aquaculture.2010.02.007.View ArticleGoogle Scholar
- Boutin S, Bernatchez L, Audet C, Derôme N: Antagonistic effect of indigenous skin bacteria of brook charr (Salvelinus fontinalis) against Flavobacterium columnare and F. psychrophilum. Vet Microbiol. 2012, 155: 355-361. 10.1016/j.vetmic.2011.09.002.PubMedView ArticleGoogle Scholar
- Arias CR, Cai W, Peatman E, Bullard SA: Catfish hybrid Ictalurus punctatus × I. furcatus exhibits higher resistance to columnaris disease than the parental species. Dis Aquat Organ. 2012, 100: 77-81. 10.3354/dao02492.PubMedView ArticleGoogle Scholar
- LaFrentz BR, Shoemaker CA, Booth NJ, Peterson BC, Ourth DD: Spleen index and mannose-binding lectin levels in four channel catfish families exhibiting different susceptibilities to Flavobacterium columnare and Edwardsiella ictaluri. J Aquatic Anim Health. 2012, 24: 141-147. 10.1080/08997659.2012.675936.View ArticleGoogle Scholar
- Snieszko SF: Columnaris disease of fishes. USFWS Fish Leafl. 1958, 46: 1-3.Google Scholar
- Amend DF, Ross AJ: Experimental control of columnaris disease with a new nitrofuran drug, P-7138. Prog Fish-Cult. 1970, 32: 19-25. 10.1577/1548-8640(1970)32[19:ECOCDW]2.0.CO;2.View ArticleGoogle Scholar
- Ross AJ: In vitro studies with nifurpirinol (P-7138) and bacterial fish pathogens. Prog Fish-Cult. 1972, 34: 18-20. 10.1577/1548-8640(1972)34[18:IVSWNP]2.0.CO;2.View ArticleGoogle Scholar
- Shiraki K, Miyamoto F, Sato T, Sonezaki I, Yano K: Studies on a new chemotherapeutic agent nifurprazine (HB-115) against fish infectious diseases. Fish Pathol. 1970, 4: 130-137. 10.3147/jsfp.4.130.View ArticleGoogle Scholar
- Deufel J: Prophylactic measures against bacterial diseases of salmonid fry (Prophylaktische Massnahmen gegen bakterielle Erkrankungen der Salmonidenbrut). Osterr Fisch. 1974, 27: 1-5.Google Scholar
- Endo T, Ogishima K, Hayasaka H, Kaneko S, Ohshima S: Application of oxolinic acid as a chemotherapeutic agent against infectious diseases in fishes-I. Antibacterial activity, chemotherapeutic effects and pharmacokinetics of oxolinic acid in fishes. Bull Jpn Soc Sci Fish. 1973, 39: 165-171. 10.2331/suisan.39.165.View ArticleGoogle Scholar
- Soltani M, Shanker S, Munday BL: Chemotherapy of Cytophaga/Flexibacter-like bacteria (CFLB) infections in fish: studies validating clinical efficacies of selected antimicrobials. J Fish Dis. 1995, 18: 555-565. 10.1111/j.1365-2761.1995.tb00360.x.View ArticleGoogle Scholar
- Wood JW: Treatment and drugs. Diseases of Pacific Salmon: their prevention and treatment. 1974, State of Washington: Department of Fisheries, Hatchery Division, 7-16. 2Google Scholar
- Koski P, Hirvelä-Koski V, Bernardet JF: Flexibacter columnaris infection in arctic char (Salvelinus alpinus (L.)). First isolation in Finland. Bull Eur Ass Fish Pathol. 1993, 31: 66-69.Google Scholar
- Wolf K, Snieszko SF: Uses of antibiotics and other antimicrobials in therapy of diseases of fishes. Antimicrob Agents Chemother. 1963, 161: 597-603.PubMedGoogle Scholar
- Gaunt PS, Gao D, Sun F, Endris R: Efficacy of florfenicol for control of mortality caused by Flavobacterium columnare infection in channel catfish. J Aquat Anim Health. 2010, 22: 115-122. 10.1577/H09-057.1.PubMedView ArticleGoogle Scholar
- Serrano PH: Responsible use of antibiotics in aquaculture. FAO Fish Tech Pap. 2005, 469: 1-97.Google Scholar
- Declercq AM, Boyen F, Van den Broeck W, Bossier P, Karsi A, Haesebrouck F, Decostere A: Antimicrobial susceptibility pattern of Flavobacterium columnare isolates collected worldwide from 17 fish species. J Fish Dis. 2013, 36: 45-55. 10.1111/j.1365-2761.2012.01410.x.PubMedView ArticleGoogle Scholar
- Darwish AM, Mitchell AJ: Evaluation of diquat against an acute experimental infection of Flavobacterium columnare in channel catfish, Ictalurus punctatus (Rafinesque). J Fish Dis. 2009, 32: 401-408. 10.1111/j.1365-2761.2009.01024.x.PubMedView ArticleGoogle Scholar
- Marchand P-A, Phan T-M, Straus DL, Farmer BD, Stüber A, Meinelt T: Reduction of in vitro growth in Flavobacterium columnare and Saprolegnia parasitica by products containing peracetic acid. Aquac Res. 2012, 43: 1861-1866. 10.1111/j.1365-2109.2011.02995.x.View ArticleGoogle Scholar
- Laanto E, Sundberg L-R, Bamford JKH: Phage specificity of the freshwater fish pathogen Flavobacterium columnare. Appl Environ Microbiol. 2011, 77: 7868-7872. 10.1128/AEM.05574-11.PubMed CentralPubMedView ArticleGoogle Scholar
- Prasad Y, Arpana , Kumar D, Sharma AK: Lytic bacteriophages specific to Flavobacterium columnare rescue catfish, Clarias batrachus (Linn.) from columnaris disease. J Environ Biol. 2011, 32: 161-168.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.