Open Access

Complementary studies detecting classical bovine spongiform encephalopathy infectivity in jejunum, ileum and ileocaecal junction in incubating cattle

  • Christine Fast1,
  • Markus Keller1,
  • Anne Balkema-Buschmann1,
  • Bob Hills2 and
  • Martin H Groschup1Email author
Veterinary Research201344:123

https://doi.org/10.1186/1297-9716-44-123

Received: 6 September 2013

Accepted: 5 December 2013

Published: 21 December 2013

Abstract

Recently we have described the distribution of bovine spongiform encephalopathy (BSE) infectivity and/or PrPSc in Peyer’s patches (PP) of the small intestine of orally BSE infected cattle. In this follow-up study additional jejunal and ileal PP’s and ileocaecal-junction tissue samples from 1, 4, and 24 months post infection (mpi) were examined by mouse (Tgbov XV) bioassay. Infectivity was demonstrated in ileal PP’s 4 mpi and the distribution/extent of infectivity at 24 mpi was comparable to those seen at earlier time points, revealing no indication for a decline/clearance. These data are relevant for the definition of Specified Risk Materials in the context of the TSE legislation worldwide.

Introduction, methods, and results

Bovine spongiform encephalopathy (BSE) belongs to the group of transmissible spongiform encephalopathies (TSE) and is associated with the accumulation of a pathological isoform of a host-encoded glycoprotein, prion protein (PrPSc). PrPSc can be distinguished from its cellular progenitor by its partial proteinase K resistance and hydrophobicity which lead to the formation of fibrils in-vivo and/or in-vitro. Classical BSE was transmitted by the oral uptake of infectious feedingstuff [1] to ruminants such as bovines [2] and goats [3], felidae [4] and humans [5]. In the European Union (EU) and in a number of other countries the BSE exposure risk for humans is minimized by the removal of specified risk materials (SRM) from slaughtered cattle that may contain BSE infectivity in incubating animals. Among other tissues this regards, at least in the EU, the intestine. Several authors have described BSE infectivity and/or PrPSc accumulations in the ileum [611], but data on jejunum and colon are patchy [1012]. In a study we published two years ago [10] we have detected BSE infectivity in jejunum, ileum and ileocaecal-junction of BSE incubating cattle from 8 to 20 months post infection (mpi) with peak levels at 12 mpi. However, immunohistochemistry (IHC) results suggested also the possibility of prion amplification in animals 4 mpi and earlier as well as a time dependent variation in the detectable amounts of PrPSc up to an age of 24 mpi. This follow up study presented here therefore intended to clarify when BSE infectivity can be found in the small intestine at the earliest time point post infection and also focused on older cattle to prove whether the undulant pattern seen in IHC is reflected by varying infectivity levels. These data are important for a risk-based definition of the gut associated SRM which is needed in the currently ongoing discussion in the EU whether restrictions for the intestinal SRM tissues can be alleviated.

All infection experiments in cattle and mice described in this manuscript were approved by the competent authority of the Federal State of Mecklenburg-Western Pomerania, Germany, on the basis of national and European legislation, namely the EU council directive 86/609/EEC for the protection of animals used for experiments.

As described before [10, 13], 56 Simmental calves aged four to six months were orally inoculated with 100 g of a brain stem homogenate pooled from clinically infected BSE cattle. The infectivity load in the homogenate was 106.1 ID50/g tissues as determined by end point titration in TgbovXV mice. Eighteen calves inoculated orally with negative brain stem homogenate served as controls. Up to five animals were randomly selected, culled and necropsied after 1, 4, 8, 12 etc. mpi and a wide range of tissue samples was taken under TSE sterile conditions. Specimens from eight infected bovines killed after 1, 4, and 24 mpi and from one control animal (4 mpi) were examined here. Anamnestic data are listed in Table 1. Peyer’s patches from four localizations [10], including two samples from the jejunum and one from each the ileum and ileocaecal-junction were tested for infectivity by mouse bioassay using bovine PrP overexpressing transgenic mice (Tgbov XV) [7]. Fifteen mice per sample were inoculated intracerebrally (30 μL) with 10% tissue homogenates diluted in sterile 0.9% sodium chloride solution. During the incubation time all mice were assessed for the onset of clinical symptoms at least two times per week. Mice showing clinical signs were sacrificed and their brains tested for the accumulation of PrPSc by a PTA immunoblotting method as described before [10].
Table 1

Anamnestic data (immunohistochemical results are from[10]) and mouse bioassay results of all cattle examined

Anamnestic data

Mouse bioassay (Tgbov XV)

Cow

mpi

Clinical status

Results by IHC*

Jejunum A

Jejunum B

Ileum

Ileocaec-junct

Brain (Obex)

Ileum/Ileocaec-junct

KT32

4

Control

--

−−/−−

0/11

0/15

0/11

0/15

IT04

1

Preclinical

--

−−/−−

0/10

0/13

0/13

0/11

IT44

1

Preclinical

--

−−/−−

0/13

0/15

0/12

0/13

IT19

4

Preclinical

--

+/−−

0/12

0/13

9/15 (380 ± 65)

0/14

IT45

4

Preclinical

--

+/−−

0/15

0/14

11/13 (366 ± 46)

0/12

IT26

24

Late preclinical

+

++/−−

8/14 (440 ± 96)

0/13

14/14 (317 ± 36)

2/11 (414, 526)

IT24

24

Preclinical

--

++/−−

1/13 (533)

0/14

15/15 (328 ± 52)

12/14 (325 ± 45)

IT47

24

Preclinical

--

+++/−−

1/15 (448)

2/12 (434, 707)

13/13 (267 ± 20)

3/12 (354,536,725)

IT58

24

Preclinical

--

−−/+

0/12

0/15

2/12 (442, 445)

0/15

*All jejunal samples were negative in IHC, mpi: months post infection; IHC: Immunohistochemistry; -- negative result, +/++ mild/moderate accumulation of PrPSc, Mouse bioassay results relying on PTA-Western blot of mouse brains: the first numbers indicate the number of positive mice/inoculated mice, the number in brackets indicates the mean incubation period in days post infection and the standard deviation.

Results of the PrPSc analysis and of the bioassay are shown in Table 1. Six out of the eight BSE infected cows carried infectivity at least in one of the gut localizations. The control cow as well as the animals killed one mpi showed no signs of infectivity. The earliest time point of detection was in both cows culled at four mpi. All animals which gave positive results in the mouse bioassay revealed signs of infectivity at least in the ileal sample. Three out of the four cows sacrificed at 24 mpi had accumulated infectivity in jejunal PP’s as well as in the ileocaecal-junction. Animal IT47 (24 mpi) even had accumulated infectivity in all examined intestine samples.

Infectivity levels at the different localizations of the small intestine were unequal, with the highest levels in the ileal PP’s followed by the ileocaecal-junction and jejunal samples. For ileal PP’s (5/6) highest transmission rates (mostly between 66% and 100% of the mice affected) and shortest incubation periods (mean value 327 days, ± 56) were observed in the bioassay. Only one animal (IT58, 24 mpi) displayed sparse amounts of infectivity in this sample. More variable were the results obtained for ileocaecal-junction samples. At this location cow IT24 (24 mpi) carried infectivity levels comparable to those in the ileal PP’s, while two other cows (IT26, IT47, both 24 mpi) showed only low infectivity levels at this site, as indicated by low bioassay transmission rates (two or three mice affected having prolonged incubation periods from 354 up to 725 dpi. Bioassay results for jejunal PP’s were quite diverse with singular diseased mice per group and long incubation periods (440 up to 533 dpi) for most animals 24 mpi, and none in the animals that were sacrificed earlier. However, there was also one cow which was in the late preclinical state, IT 26 (24 mpi), where the jejunal PP’s yielded a bioassay transmission rate of more than 50% with an average incubation time of 440 days.

Discussion

In this follow-up study we have mapped the exact temporal and spatial emergence and distribution of infectivity in the PP’s of the small intestine in orally BSE infected cattle. According to IHC results described previously [10, 11], infectivity was first seen four months after the oral challenge. However in IHC only traces of PrPSc were seen in single follicles of the ileal PP’s at four mpi [10]. This is not reflected by the results presented here, showing already moderate to high amounts of infectivity comparable to levels at later stages of disease [10]. As the detection of infectivity mostly precedes the detection of PrPSc, these results support the theory that the latent period post exposure during which no detectable infection is present might be around two or three months.

Secondly we were interested in the amount and distribution of PrPSc at 24 mpi, since the extent of this accumulation varied at earlier time points with peaks at 8 and in particular 12 mpi and lows at 16 mpi respectively [10]. However, these earlier studies did also suggest a higher amount in animals at 24 mpi suggesting an undulant pattern of about 12 months. This finding is now supported by the bioassay results, as three out of the four cattle from the 24 mpi group showed levels and distribution of infectivity comparable to the peaks seen at 12 mpi. However it has to be bear in mind that only four animals per time point were investigated here and in earlier studies and that the variations between individuals are very high [10, 14]. This is reflected in the present study by variable detection rates in different animals of the 24 mpi group and might explain the differences seen in infectivity levels reported for ileal samples by several authors before [6, 911, 15].

In summary, data presented here clearly showed that infectivity is not detectable in the small intestine of animals up to four months post experimental oral exposure with an extremely high dose. Moreover, the low amounts of infectivity detectable after the peak at 12 mpi as demonstrated previously, does not imply an irreversible clearance of the infectious agent from the gut over time, but is rather a time-dependent individual fluctuation, as a higher infectivity load is seen again at 24 mpi. Hence, the data presented here are important for a risk- based SRM definition.

Author’s contributions

This study was designed by CF, BH and MHG, practically realized by CF, MK, ABB and the data were analyzed and the manuscript was written by CF and MHG. All authors read and approved the final manuscript.

Declarations

Acknowledgements

We are grateful to Ute Ziegler for her pivotal contribution to the realization of the German BSE pathogenesis study. Bärbel Hammerschmidt, Susann Jänicke and Gesine Kreplin are acknowledged for their excellent technical assistance. This work was financially supported by grants from Health Canada, the Federal Ministeries for Food, Agriculture and Consumer Protection and for Education and Research as well as from the EU Commission (Network of Excellence “Neuroprion”).

Authors’ Affiliations

(1)
Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases
(2)
Health Canada, Transmissible Spongiform Encephalopathy Secretariat

References

  1. Wilesmith JW, Wells GA, Cranwell MP, Ryan JB: Bovine spongiform encephalopathy: epidemiological studies. Vet Rec. 1988, 123: 638-644.PubMedGoogle Scholar
  2. Wells GA, Hancock RD, Cooley WA, Richards MS, Higgins RJ, David GP: Bovine spongiform encephalopathy: diagnostic significance of vacuolar changes in selected nuclei of the medulla oblongata. Vet Rec. 1989, 125: 521-524. 10.1136/vr.125.21.521.View ArticlePubMedGoogle Scholar
  3. Eloit M, Adjou K, Coulper M, Fontaine JJ, Hamel R, Lilin T, Messiaen S, Andreoletti O, Baron T, Bencsik A, Biacabe AG, Beringue V, Laude H, Le Dur A, Vilotte JL, Comoy E, Deslys JP, Grassi J, Simon S, Lantier F, Sarradin P: BSE agent signatures in a goat. Vet Rec. 2005, 156: 523-524.View ArticlePubMedGoogle Scholar
  4. Eiden M, Hoffmann C, Balkema-Buschmann A, Müller M, Baumgartner K, Groschup MH: Biochemical and immunohistochemical characterization of feline spongiform encephalopathy in a German captive cheetah. J Gen Virol. 2010, 91: 2874-2883. 10.1099/vir.0.022103-0.View ArticlePubMedGoogle Scholar
  5. Bruce ME, Will RG, Ironside JW, McConnell I, Drummond D, Suttie A, McCardle L, Chree A, Hope J, Birkett C, Cousens S, Fraser H, Bostock CJ: Transmissions to mice indicate that “new variant” CJD is caused by the BSE agent. Nature. 1997, 389: 498-501. 10.1038/39057.View ArticlePubMedGoogle Scholar
  6. Terry LA, Marsh S, Ryder SJ, Hawkins SA, Wells GA, Spencer YI: Detection of disease specific PrP in the distal ileum of cattle exposed orally to the agent of bovine spongiform encephalopathy. Vet Rec. 2003, 152: 387-392. 10.1136/vr.152.13.387.View ArticlePubMedGoogle Scholar
  7. Buschmann A, Groschup MH: Highly bovine spongiform encephalopathysensitive transgenic mice confirm the essential restriction of infectivity to the nervous system in clinically diseased cattle. J Infect Dis. 2005, 192: 934-942. 10.1086/431602.View ArticlePubMedGoogle Scholar
  8. Wells GA, Spiropoulos J, Hawkins SA, Ryder SJ: Pathogenesis of experimental bovine spongiform encephalopathy: preclinical infectivity in tonsil and observations on the distribution of lingual tonsil in slaughtered cattle. Vet Rec. 2005, 156: 401-407.View ArticlePubMedGoogle Scholar
  9. Espinosa JC, Morales M, Castilla J, Rogers M, Torres JM: Progression of prion infectivity in asymptomatic cattle after oral bovine spongiform encephalopathy challenge. J Gen Virol. 2007, 88: 1379-1383. 10.1099/vir.0.82647-0.View ArticlePubMedGoogle Scholar
  10. Hoffmann C, Eiden M, Kaatz M, Keller M, Ziegler U, Rogers R, Hills B, Balkema-Buschmann A, van Keulen L, Jacobs JG, Groschup MH: BSE infectivity in jejunum, ileum and ileocaecal junction of incubating cattle. Vet Res. 2011, 42: 21-10.1186/1297-9716-42-21.PubMed CentralView ArticlePubMedGoogle Scholar
  11. Stack MJ, Moore SJ, Vidal-Diez A, Arnold ME, Jones EM, Spencer YI, Webb P, Spiropoulos J, Powell L, Bellerby P, Thurston L, Cooper J, Chaplin MJ, Davis LA, Everitt S, Focosi-Snyman R, Hawkins SA, Simmons MM, Wells GA: Experimental bovine spongiform encephalopathy: detection of PrPSc in the small intestine relative to exposure dose and age. J Comp Pathol. 2011, 145: 289-301. 10.1016/j.jcpa.2011.01.010.View ArticlePubMedGoogle Scholar
  12. Okada H, Iwamaru Y, Imamura M, Masujin K, Yokoyama T, Mohri S: Immunohistochemical detection of disease-associated prion protein in the intestine of cattle naturally affected with bovine spongiform encephalopathy by using an alkaline-based chemical antigen retrieval method. J Vet Med Sci. 2010, 72: 1423-1429. 10.1292/jvms.10-0211.View ArticlePubMedGoogle Scholar
  13. Hoffmann C, Ziegler U, Buschmann A, Weber A, Kupfer L, Oelschlegel A, Hammerschmidt B, Groschup MH: Prions spread via the autonomic nervous system from the gut to the central nervous system in cattle incubating bovine spongiform encephalopathy. J Gen Virol. 2007, 88: 1048-1055. 10.1099/vir.0.82186-0.View ArticlePubMedGoogle Scholar
  14. Kaatz M, Fast C, Ziegler U, Balkema-Buschmann A, Hammerschmidt B, Keller M, Oelschlegel A, McIntyre L, Groschup MH: Spread of classic BSE prions from the gut via the peripheral nervous system to the brain. Am J Pathol. 2012, 181: 515-524. 10.1016/j.ajpath.2012.05.001.View ArticlePubMedGoogle Scholar
  15. Wells GA, Hawkins SA, Green RB, Austin AR, Dexter I, Spencer YI, Chaplin MJ, Stack MJ, Dawson M: Preliminary observations on the pathogenesis of experimental bovine spongiform encephalopathy (BSE): an update. Vet Rec. 1998, 142: 103-106. 10.1136/vr.142.5.103.View ArticlePubMedGoogle Scholar

Copyright

© Fast et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.