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Table 1 Anamnestic data (immunohistochemical results are from[10]) and mouse bioassay results of all cattle examined

From: Complementary studies detecting classical bovine spongiform encephalopathy infectivity in jejunum, ileum and ileocaecal junction in incubating cattle

Anamnestic data

Mouse bioassay (Tgbov XV)

Cow

mpi

Clinical status

Results by IHC*

Jejunum A

Jejunum B

Ileum

Ileocaec-junct

Brain (Obex)

Ileum/Ileocaec-junct

KT32

4

Control

--

−−/−−

0/11

0/15

0/11

0/15

IT04

1

Preclinical

--

−−/−−

0/10

0/13

0/13

0/11

IT44

1

Preclinical

--

−−/−−

0/13

0/15

0/12

0/13

IT19

4

Preclinical

--

+/−−

0/12

0/13

9/15 (380 ± 65)

0/14

IT45

4

Preclinical

--

+/−−

0/15

0/14

11/13 (366 ± 46)

0/12

IT26

24

Late preclinical

+

++/−−

8/14 (440 ± 96)

0/13

14/14 (317 ± 36)

2/11 (414, 526)

IT24

24

Preclinical

--

++/−−

1/13 (533)

0/14

15/15 (328 ± 52)

12/14 (325 ± 45)

IT47

24

Preclinical

--

+++/−−

1/15 (448)

2/12 (434, 707)

13/13 (267 ± 20)

3/12 (354,536,725)

IT58

24

Preclinical

--

−−/+

0/12

0/15

2/12 (442, 445)

0/15

  1. *All jejunal samples were negative in IHC, mpi: months post infection; IHC: Immunohistochemistry; -- negative result, +/++ mild/moderate accumulation of PrPSc, Mouse bioassay results relying on PTA-Western blot of mouse brains: the first numbers indicate the number of positive mice/inoculated mice, the number in brackets indicates the mean incubation period in days post infection and the standard deviation.