ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Table 3 The primers employed in the present study

Primer	Sequence (5′-3′^a)	Usage
pGL3-5'UTR-F	cgggctcgagatctATGACGTATAGGTGTTGGCTCTATGC	Amplification of PRRSV 5'UTR
pGL3-5'UTR-R	cggaatgccaagcttGGTTAAAGGGGTGGAGAGACC	Amplification of PRRSV 5'UTR
pGL3-5'-3'UTR-F	aagatcgccgtgtaaTGGGCTGGCATTCTTTGG	Amplification of PRRSV 3'UTR
pGL3-5'-3'UTR-R	tcggtcgacggatccTTTTTTAATTGCGGCCGCATGGT
pGL3-3'UTR-R	cgccccgactctagaTTTTTTAATTGCGGCCGCATGG
q-mZNF283-F	TCCATGGCATAACTTGGGGAA	qRT-PCR for detection of Marc-145 ZNF283
q-mZNF283-R	TGGCCATGCATTACACAAACTG
q-sZNF283-F	CGAGTTTGCCTGAGAAGAGCG	qRT-PCR for detection of PAM ZNF283
q-sZNF283-R	GAAGACACGGGAAAGGGACG
q-mGAPDH-F	TGATGACATCAAGAAGGTGGTGAAG	qRT-PCR for detection of Marc-145 GAPDH
q-mGAPDH-R	TCCTTGGAGGCCATGTGGGCCAT
q-sGAPDH-F	CCTTCCGTGTCCCTACTGCCAAC	qRT-PCR for detection of PAM GAPDH
q-sGAPDH-R	GACGCCTGCTTCACCACCTTCT

^aThe DNA sequences homologous to vector regions are depicted in lowercase letters, and the gene-specific regions are shown in uppercase letters.

ISSN: 1297-9716