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Table 2 The shRNA sequences used in the present study

From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

shRNA

Sequence (5'-3'a)

shmZNF283-1-F

ccggGCATCCATCTTCAGAAATAATCTCGAGATTATTTCTGAAGATGGATGCTTTTTG

shmZNF283-1-R

aattcAAAAAGCATCCATCTTCAGAAATAATCTCGAGATTATTTCTGAAGATGGATGC

shmZNF283-2-F

ccggGCCTCGCTAAACATGAGATAACTCGAGTTATCTCATGTTTAGCGAGGCTTTTTG

shmZNF283-2-R

aattcAAAAAGCCTCGCTAAACATGAGATAACTCGAGTTATCTCATGTTTAGCGAGGC

shmZNF283-3-F

ccggGGCCTTTGGTAGTGGCTATCACTCGAGTGATAGCCACTACCAAAGGCCTTTTTG

shmZNF283-3-R

aattcAAAAAGGCCTTTGGTAGTGGCTATCACTCGAGTGATAGCCACTACCAAAGGCC

scrambled shRNA-F

ccggGAAGAGGACACGCCTTAGACTCTCGAGAGTCTAAGGCGTGTCCTCTTCTTTTTG

scrambled shRNA-R

aattcAAAAAGAAGAGGACACGCCTTAGACTCTCGAGAGTCTAAGGCGTGTCCTCTTC

  1. aFlanking sequences (lowercase letters) represent the AgeI/EcoRI restriction sites. shRNA target sequences are shown in uppercase letters.
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Veterinary Research

ISSN: 1297-9716