Table 1 The primers used in the present study
Primer | Sequence (5′-3′a) | Usage |
|---|---|---|
HA-ZNF283-F | atcattttggcaaagATGAGCCTCTGGGCCCA | Amplification of ZNF283 and 1–191/296/408/527 |
HA-ZNF283-R | tgaaccgcctccaccTAAAGTTTCAGCAGCATCGACTGTCTCA | Amplification of ZNF283 and 192–663 |
HA-ZNF283 1–191-R | tgaaccgcctccaccAATGTAGGGTTTCTCTTGCTTATGAATAC | Amplification of ZNF283 1–191 |
HA-ZNF283 1–296-R | tgaaccgcctccaccTTCATAAGACTTTATGCCTATATGAATTTTCTGATG | Amplification of ZNF283 1–296 |
HA-ZNF283 1–408-R | tgaaccgcctccaccATGGATTCTCTCATGCTGAACCA | Amplification of ZNF283 1–408 |
HA-ZNF283 1–527-R | tgaaccgcctccaccTCCGTAGGGCCTCTCACC | Amplification of ZNF283 1–527 |
HA-ZNF283 192–663-F | atcattttggcaaagATGTGTCAGGAATGTGAGAAGGCTGG | Amplification of ZNF283 192–663 |
EGFP-ZNF283 56–97-F | gtaccgcgggcccgggatccTCGGTGACCTTCAAGGATGTG | Amplification of ZNF283 56–97 |
EGFP-ZNF283 56–97-R | tcagttatctagatccggtgctaATCCAGCGAGACCAAGTTGC | |
Flag-Nsp9-F | atcattttggcaaagATGTTTAAACTGCTAGCCGCCAGC | Amplification of Nsp9 and 1–177/449 |
Flag-Nsp9-R | tgaaccgcctccaccCTCATGATTGGACCTGAGTTTTTCCC | Amplification of Nsp9 and 178/450–643 |
Flag-Nsp9 1–177-R | tgaaccgcctccaccGCTTCCAGTGTCACTGGGG | Amplification of Nsp9 1–177 |
Flag-Nsp9 178–449-F | atcattttggcaaagATGCCGGTGCACGCGG | Amplification of Nsp9 178–449/643 |
Flag-Nsp9 178–449-R | tgaaccgcctccaccGCCACCTCTCTTAGTCACCGC | Amplification of Nsp9 1/178–449 |
Flag-Nsp9 450–643-F | atcattttggcaaagATGCTGTCGTCTGGCGACCC | Amplification of Nsp9 450–643 |
Flag-Nsp10-F | atcattttggcaaagATGGGGAAGAAGTCCAGAATGTGCG | Amplification of Nsp10 |
Flag-Nsp10-R | tgaaccgcctccaccTTCCAAGTCTGCGCAAATAGCG | |
Flag-Nsp11-F | atcattttggcaaagATGGGGTCGAGCTCCCCG | Amplification of Nsp11 |
Flag-Nsp11-R | tgaaccgcctccaccTTCAAGTTGAAAATAGGCCGTCTTGTCT | |
Flag-Nsp12-F | atcattttggcaaagATGGGCCGCCATTTTACCTGG | Amplification of Nsp12 |
Flag-Nsp12-R | tgaaccgcctccaccATTCAGGCCTAAAGTTGGTTCAATGAC |