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Figure 9 | Veterinary Research

Figure 9

From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Figure 9

PRRSV Nsp10 interacts with the ZNF283 KRAB domain from aa 56–97. A Marc-145 cells were cotransfected with Flag-tagged Nsp10 and EGFP-tagged ZNF283 truncations (aa 56–97) or HA-tagged ZNF283 truncations (aa 192–663) for 24 h. The cells were fixed and immunostained using either mouse anti-Flag or rabbit anti-HA antibodies. Nuclei were counterstained with DAPI. B HEK-293T cells were cotransfected with Flag-tagged Nsp10 and HA-tagged ZNF283, EGFP-tagged ZNF283 truncations (aa 56–97), or HA-tagged ZNF283 truncations (aa 192–663) for 36 h. After cell lysis, immunoprecipitation analysis was performed using a mouse anti-Flag antibody.

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Veterinary Research

ISSN: 1297-9716