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Figure 7 | Veterinary Research

Figure 7

From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Figure 7

ZNF283 interacts with the aa 178–449 region of Nsp9. A Schematic representation of Nsp9 truncation mutants. B Marc-145 cells were cotransfected with HA-tagged ZNF283 and plasmid-encoded Flag-tagged Nsp9 truncations (aa 1–177, 178–449, 450–643, 1–499, and 178–643) for 24 h. The cells were fixed and immunostained with mouse anti-Flag and rabbit anti-HA antibodies. Nuclei were detected by counterstaining with DAPI. C HEK-293T cells were cotransfected with the HA-tagged ZNF283 plasmid and Flag-tagged truncated Nsp9 (aa 178–449) for 36 h. Immunoprecipitation of cell lysates was performed using either mouse anti-Flag or mouse anti-HA antibodies, followed by Western blotting analysis using either rabbit anti-HA or rabbit anti-Flag antibodies.

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Veterinary Research

ISSN: 1297-9716