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Figure 6 | Veterinary Research

Figure 6

From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Figure 6

PRRSV Nsp9 interacts with the ZNF283 KRAB domain from aa 56–97. A Diagrammatic illustration of diverse fragments of ZNF283. B Marc-145 cells were cotransfected with Flag-tagged Nsp9 and plasmid-encoded HA-tagged ZNF283 truncations (aa 1–191, 1–296, 1–408, 1–520, and 192–663) or EGFP-tagged ZNF283 aa 56–97 for 24 h. The cells were fixed and immunostained using either a mouse anti-Flag or rabbit anti-HA antibody. Nuclei were counterstained with DAPI. C HEK-293T cells were cotransfected with the plasmids Flag-tagged Nsp9 and full-length HA-tagged ZNF283, EGFP-tagged ZNF283 truncations (aa 56–97), or HA-tagged ZNF283 truncations (aa 192–663) for 36 h. Following cell lysis, immunoprecipitation analysis was conducted using a mouse anti-Flag antibody.

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Veterinary Research

ISSN: 1297-9716