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Figure 1 | Veterinary Research

Figure 1

From: ZNF283, a Krüppel-associated box zinc finger protein, inhibits RNA synthesis of porcine reproductive and respiratory syndrome virus by interacting with Nsp9 and Nsp10

Figure 1

The expression of ZNF283 is upregulated by PRRSV infection. Marc-145 cells and PAMs were mock-infected or infected with PRRSV at an MOI of 0.2 for various durations. The cells were then collected and analysed for ZNF283 mRNA and protein expression levels using RT‒qPCR (A and C) and Western blotting (B and D), respectively. E Marc-145 cells were transfected with HA-tagged ZNF283 for 24 h and then infected with PRRSV at an MOI of 0.2. Mock-infected cells served as controls. At 24 h post-infection, the cells were fixed and immunostained with mouse anti-N protein and rabbit anti-HA antibodies. Nuclei were counterstained with DAPI. F Marc-145 cells were transfected with HA-ZNF283 for 24 h and then infected with PRRSV at an MOI of 0.2. At 36 h post-infection, cellular fractionation followed by Western blotting was performed. HDAC1 and GAPDH were used as control proteins for the nuclear and cytosolic fractions, respectively.

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Veterinary Research

ISSN: 1297-9716