Figure 2

Siderophore-associated protein homology in diverse NASM isolates. Presence of siderophore-associated proteins within S. chromogenes isolates [from a persistent intramammary infection (IM), the teat apex of a dairy heifer (TA), composite cow milk (CCM) and bulk tank milk (BTM)] and S. equorum isolates (from CCM and BTM) highlighted in pink, including 100 isolates (S. chromogenes = 83, S. equorum = 17) from the Mastitis Pathogen Collection of the Canadian Bovine Mastitis and Milk Quality Research Network (CBMQRN) [35], and quality control reference strain S. aureus ATCC 25923. An ML tree (1000 ultrafast bootstraps) representing phylogenetic relationship of S. chromogenes, S. equorum, and quality control strain S. aureus ATCC 25923 on whole genome SNP level with the S. aureus ATCC 25923 (CP009361) as reference. The final phylogenetic tree was annotated with the presence of siderophore- and ferritin-related protein hits across genomes. Color coded (blue-red) represents amino acid homology of the identified proteins as compared to the NCBI siderophore- and ferritin-related protein hits. Only hits with amino acid homology above 30% and 50% query coverage are shown.