Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice

Table 1 Primer sequences and the names of PCR products and generated vectors used in this study

Primer name	Sequence (5ʹ to 3ʹ)	PCR product name	Vector name
S1-1332-noATG-F	ACGCCTTTAATTTACTTCTGGTTGTTCTTAC	PEDV/S(1–1332)-XhoI	pENTR11L21-PEDV/S(1–1332) + TEVdH8S
S1-1332-stopXhoI-F	GGGGCTCGAGATAAAAATAATCAACCAAACCCAC	PEDV/S(1–1332)-XhoI	pENTR11L21-PEDV/S(1–1332) + TEVdH8S
S1-1332-F	AAGCACAAAGGAAGCTAGACCCAGCTTTCTTGTACAAAG	PEDV/S(1–1332)-Tags	pENTR11L21-PEDV/S(1–1332) + Tags
S1-1332-R	CATGCTGCCCCCTCCATGATGATGGTGATGATGCTTTTCG	PEDV/S(1–1332)-Tags	pENTR11L21-PEDV/S(1–1332) + Tags
S1-1320-F	TCGAGTGCAGGCGAAAACCTGTACTTCC	PEDV/S(1–1320)-Tags	pENTR11L21-PEDV/S(1–1320) + Tags
S1-1320-R	GATATATGTCTCAACTCGGTTGAGCCACTCAAGG	PEDV/S(1–1320)-Tags	pENTR11L21-PEDV/S(1–1320) + Tags
PEDVS-TEV-F	TCGAGTGCAGGCGAAAACCTGTACTTCC	PEDV/S(1–1320)-Tags-gib	pENTR11L21-PEDV/S(1–1320) + CMP + Tags
S+CMP-R	GATATATGTCTCAACTCGGTTGAGCCAC	PEDV/S(1–1320)-Tags-gib
CMP-F	CCGAGTTGAGACATATATCGAAGAAGACCCGTGTGAGTGTAAAAGC	CMP
CMP-R	GGTTTTCGCCTGCACTCGAGATAATTTTATTCTCCAGCGCTTCAATGCG	CMP

ISSN: 1297-9716