Table 1 Primer sequences and the names of PCR products and generated vectors used in this study
Primer name | Sequence (5ʹ to 3ʹ) | PCR product name | Vector name |
|---|---|---|---|
S1-1332-noATG-F | ACGCCTTTAATTTACTTCTGGTTGTTCTTAC | PEDV/S(1–1332)-XhoI | pENTR11L21-PEDV/S(1–1332) + TEVdH8S |
S1-1332-stopXhoI-F | GGGGCTCGAGATAAAAATAATCAACCAAACCCAC | ||
S1-1332-F | AAGCACAAAGGAAGCTAGACCCAGCTTTCTTGTACAAAG | PEDV/S(1–1332)-Tags | pENTR11L21-PEDV/S(1–1332) + Tags |
S1-1332-R | CATGCTGCCCCCTCCATGATGATGGTGATGATGCTTTTCG | ||
S1-1320-F | TCGAGTGCAGGCGAAAACCTGTACTTCC | PEDV/S(1–1320)-Tags | pENTR11L21-PEDV/S(1–1320) + Tags |
S1-1320-R | GATATATGTCTCAACTCGGTTGAGCCACTCAAGG | ||
PEDVS-TEV-F | TCGAGTGCAGGCGAAAACCTGTACTTCC | PEDV/S(1–1320)-Tags-gib | pENTR11L21-PEDV/S(1–1320) + CMP + Tags |
S+CMP-R | GATATATGTCTCAACTCGGTTGAGCCAC | ||
CMP-F | CCGAGTTGAGACATATATCGAAGAAGACCCGTGTGAGTGTAAAAGC | CMP | |
CMP-R | GGTTTTCGCCTGCACTCGAGATAATTTTATTCTCCAGCGCTTCAATGCG |