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Table 1 Primers used in this study

From: SYNCRIP facilitates porcine parvovirus viral DNA replication through the alternative splicing of NS1 mRNA to promote NS2 mRNA formation

Primers

Sequences (5′–3′)

Vectors

NS1-F

CACTCGAGATGGATTACAAGGATGACGACGATAAGGCAGCGGGAAACACTTACTC

pCI-neo

NS1-R

GGCTCTAGATTATTCAAGGTTTGTTGTGGGTG

p-NS1-F

CACTCGAGATGGCAGCGGGAAACACTTAC

pEGFP-N1

p-NS1-R

GGCGAATTCGCATTCAAGGTTTGTTGTGGGTG

NS2-F

CACTCGAGATGGCAGCGGGAAACACTTAC

pEGFP-N1

NS2-R

GTCGGATCCGTCCAAAGCAGGCTCTTATG

p-NS2-F

CAGCTAGCATGGCAGCGGGAAACACTTAC

pCDH-EF1-MCS-T2A-Puro

p-NS2-R

GTCGCGGCCGCGTCCAAAGCAGGCTCTTATG

VP1-F

CACTCGAGATGGCGCCTCCTGCAAAAAGAGCAAGAGGACTAACTCTACCAGGATAC

pEGFP-N1

VP1-R

GTCGGATCCGTGTATAATTTTCTTGGTATAAG

VP2-F

CACTCGAGATGAGTGAAAATGTGGAAC

pEGFP-N1

VP2-R

GTCGGATCCGTGTATAATTTTCTTGGTATAAG

SYNCRIP-F

GGCGTCGACTCATGGCTACAGAACATGTTAATGGA

pGEX-4 T-1

SYNCRIP-R

GGCGCGGCCGCTTATTGTAACAGGTCAGGACCGG

p-SYNCRIP-F

GGCCTCGAGATGGCTACAGAACATGTTAATGGA

pCDNA3.0

p-SYNCRIP-R

GGCTCTAGATTAATGGTGATGGTGATGATGGGTGGAGGCTTGTAACAGGTCAGGACCGG

  1. Restriction enzyme sequences are underlined.