Figure 7
SYNCRIP affects NS1 expression by acting on the 3′-terminal site of NS2 mRNA. A A schematic diagram of NS1 mRNA mutation site. B Western blotting analysis of the expression of different NS1 mutants in PK-15SYNCRIP−/− cells. C Western blotting analysis of viral parental NS1, NS1pNmut, NS1pCmut and NS1pNpCmut protein expression levels in PK-15 cells with SYNCRIP over-expression. D Western blotting analysis of NS1pCmut protein levels (upper) and mRNA levels (lower) in PK-15 cells co-transfected with different doses of SYNCRIP for 24 h. E Western blotting analysis of NS1pNpCmut protein levels (upper) and mRNA levels (lower) in PK-15 cells co-transfected with different doses of SYNCRIP for 24 h. F Western blotting analysis of NS1pNmut protein levels (upper) and mRNA levels (lower) in PK-15 cells co-transfected with different doses of SYNCRIP for 24 h. G The effects of NS1pCmut mutation on PPV DNA copy number were determined by q-PCR assay. (H) The effects of NS1pCmut mutation on PPV viral titer were determined by TCID50 assay. The results are shown as the mean ± SD (n = 3). **p < 0.01 versus PK-15 cells with the same treatment at the same time points.