Figure 5
Iron content in head kidney and plasma of Oncorhynchus and gene expression of iron metabolism markers after Deferiprone treatment. A Determination of iron in head kidney (µg iron per mg dry weight). Iron levels in non-challenged and untreated (DFP −, white box) or DFP treated (DFP + , blue box) head kidneys were measure at S5 sampling points. Data show box and whisker plot and solid horizontal lines indicate the median and the 25th 75th interquartile range level of at least four fish in two independent experiments. B Gene expression analysis performed in non-challenged and untreated (DFP −, white circles) or DFP treated (DFP + , blue circles) fish at S5 sampling point (30 days of treatment). Transferrin receptor (TfR), ferritin (ferH) and iron-regulated transporter 1 (ireg1) genes were quantified relative to elongation factor 1-alpha a housekeeping gene (AU, arbitrary units). Data show mean ± SD of six fish in triplicates measured in at least two independent experiments. C Quantification of plasmatic iron levels in non-challenged and untreated (DFP −, white box) or DFP treated (DFP + , blue box) fish during the course of the experiment at different S0 to S5 sampling points. Data show box and whisker plot and solid horizontal lines indicate the median and the 25th 75th interquartile range level of at least four fish in two independent experiments. For A–C, unpaired t-test was performed; asterisks show statistically significant differences between DFP − and DFP + group denote: *p-value < 0.05, ****p-value < 0.0001.