EGTA treatment increases the binding of rotavirus to enterocytes. Cells were pre-incubated with PBS or EGTA and inoculated with rotavirus at 4 °C for 1 h. After removing the unbound viral particles, cells were further incubated for 9 h. A Representative confocal image of infection in enterocytes. Scale bar: 50 µm. The percentage of infected cells (B) and the virus titer (C) were determined. Data are expressed as the mean ± SD of the results of three separate experiments. Statistically significant differences are indicated with one asterisk (p < 0.05) and two asterisks (p < 0.01).