Figure 1

Yeast two-hybrid (Y2H) and pulldown assays. A Y2H. pGADT7-FaeG and pGBKT7-APN were co-expressed in yeast and positive clones were tested for β-galactosidase activity. Samples 1–3: F4ab FaeG-APN; Samples 4–6: F4ac FaeG-APN; Samples 7–9: F4ad FaeG-APN; N: negative control; P: positive control. B GST-pulldown assays. The binding between the recombinant FaeG and APN proteins was studied using the Pierce™ GST Protein Interaction Pull-Down Kit. Western blotting with anti-F4 monoclonal antiserum and anti-APN polyclonal antiserum was used for detection. C Metaperiodate treatment. PVDF membranes were treated with either 0, 10, or 20 mM NaIO₄ before using the membranes in Western blots as described in B. Each experiment was repeated three times and representative results are shown.