Ovine and murine T cell epitopes from the non-structural protein 1 (NS1) of bluetongue virus serotype 8 (BTV-8) are shared among viral serotypes

Table 1 Prediction of H-2 ^b binding peptides from NS1 and binding assays

				Predictive algorithm scores
Position	Name	Sequence	Predicted allele binding	SYFPEITHI score (D^b/K^b)	NetMHC predicted affinity nM (D^b/K^b)	BIMAS proPred-I score (D^b/K^b)	Binding assay* (D^b/K^b)
14	NS1(14)	YANATRTFL	D^b	16	341	17.892	+
125	NS1(125)	SALVNSERV	D^b	28	10	138.526	+++
152	NS1(152)	GQIVNPTFI	D^b	28	5	1502.928	+++
222	NS1(222)	IQLINFLRM	D^b/K^b	22/17	239/201	226.359/11.000	++/++
403	NS1(403)	NCYTGAEAL	D^b/K^b	14/11	21953/18590	3.982/6.000	+/-
141	NS1(141)	AMPYIYVPI	K^b	22	137	19.800	+
166	NS1(166) Cl I	IAYYFYNPD	K^b	19	30	1.320	+++
235	NS1(235)	KHFNRYASM	K^b	17	22	40.000	+++
166	NS1(166) Cl II	IAYYFYNPDAADDWI	I-A^b	NA	194.8	NA	NA
402	NS1(402)	TNCYTGAEALITTAI	I-A^b	NA	87.3	NA	NA
522	NS1(522)	KVHFAGFAAPACESG	I-A^b	NA	59.1	NA	NA

NA: Not available.
*Peptides were ranked relative to the binding of the control peptide gp33-41 from LCMV to Db and Kb molecules on RMA-s cells (fluorescence ratio between NS1 peptide and gp33-41 peptide) (Rojas et al. [21]). +++: strong binder where ratio > 0.9; ++: moderate binder where ratio > 0.7 and < 0.9; +: weak binder where ratio > 0.5 and < 0.7; and -: no binder where ratio < 0.5.

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