Strains | Features | Source of reference |
---|---|---|
Escherichia coli | ||
DH5α | F-Φ80lacZ∆M15∆(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ- | Laboratory collection |
EC100D pir-116 | F-mcrA∆(mrr-hsdRMS-mcrBC) Φ 80dlacZ∆M15 ∆lacX74 recA1 endA1 araD139 ∆(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFR) | Laboratory collection |
EC100D pir-116/R995 + SPI-6 | Strain carrying the T6SSSPI-6 from S. Typhimurium cloned in plasmid R995 | This study |
EC100D pir-116/R995 + SPI-19 | Strain carrying the T6SSSPI-19 from S. Gallinarum cloned in plasmid R995 | [25] |
DH5α/R995 | Strain harboring an empty R995 vector | This study |
DH5α/R995-VC6 | Strain harboring plasmid R995-VC6 | This study |
Salmonella Dublin | ||
CT_02021853 | Wild-type strain | Laboratory collection |
MSD753 | CT_02021853 ∆phoN::FRT | This study |
MSD35 | CT_02021853 ∆T6SSSPI-6 ∆T6SSSPI-19 | This study |
MSD36 | CT_02021853 ∆T6SSSPI-6 | This study |
MSD37 | CT_02021853 ∆T6SSSPI-19 | This study |
WT/R995 | CT_02021853 containing an empty R995 vector | This study |
MSD35R | MSD35 harboring R995 plasmid | This study |
MSD35R6 | MSD35 complemented with plasmid R995 + SPI-6 | This study |
MSD35R19 | MSD35 complemented with plasmid R995 + SPI-19 | This study |
Plasmids | ||
pKD46 | bla PBADbet exo pSC101 oriTs, AmpR | [26] |
pCLF2 | Red-swap redesigned vector, CamR | [27] |
pCLF4 | Red-swap redesigned vector, KanR | [27] |
pEKA30 | IncQ plasmid that constitutively express Cre recombinase, AmpR | [28] |
pVEX1212 | Suicide vector harboring a loxP site followed by a SpR cassette | [28] |
pVEX2212 | Suicide vector harboring a loxP site followed by a CamR cassette | [28] |
R995 | Self-transmissible broad-host range IncP vector | [28] |
R995-VC6 | A derivative of plasmid R995 with a cloned 1,209 bp DNA fragment of T6SSSPI-6 from S. Dublin | This study |
R995 + SPI-6 | T6SSSPI-6 cluster from S. Dublin cloned in vector R995 | This study |
R995 + SPI-19 | T6SSSPI-19 cluster from S. Gallinarum 287/91 cloned in vector R995 | [25] |