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Table 1 Strains and plasmids used in this study

From: Only one of the two type VI secretion systems encoded in the Salmonella enterica serotype Dublin genome is involved in colonization of the avian and murine hosts

Strains Features Source of reference
Escherichia coli   
DH5α F-Φ80lacZ∆M15∆(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ- Laboratory collection
EC100D pir-116 F-mcrA∆(mrr-hsdRMS-mcrBC) Φ 80dlacZ∆M15 ∆lacX74 recA1 endA1 araD139 ∆(ara, leu)7697 galU galK λ- rpsL (StrR) nupG pir-116(DHFR) Laboratory collection
EC100D pir-116/R995 + SPI-6 Strain carrying the T6SSSPI-6 from S. Typhimurium cloned in plasmid R995 This study
EC100D pir-116/R995 + SPI-19 Strain carrying the T6SSSPI-19 from S. Gallinarum cloned in plasmid R995 [25]
DH5α/R995 Strain harboring an empty R995 vector This study
DH5α/R995-VC6 Strain harboring plasmid R995-VC6 This study
Salmonella Dublin   
CT_02021853 Wild-type strain Laboratory collection
MSD753 CT_02021853 ∆phoN::FRT This study
MSD35 CT_02021853 ∆T6SSSPI-6 ∆T6SSSPI-19 This study
MSD36 CT_02021853 ∆T6SSSPI-6 This study
MSD37 CT_02021853 ∆T6SSSPI-19 This study
WT/R995 CT_02021853 containing an empty R995 vector This study
MSD35R MSD35 harboring R995 plasmid This study
MSD35R6 MSD35 complemented with plasmid R995 + SPI-6 This study
MSD35R19 MSD35 complemented with plasmid R995 + SPI-19 This study
Plasmids   
pKD46 bla PBADbet exo pSC101 oriTs, AmpR [26]
pCLF2 Red-swap redesigned vector, CamR [27]
pCLF4 Red-swap redesigned vector, KanR [27]
pEKA30 IncQ plasmid that constitutively express Cre recombinase, AmpR [28]
pVEX1212 Suicide vector harboring a loxP site followed by a SpR cassette [28]
pVEX2212 Suicide vector harboring a loxP site followed by a CamR cassette [28]
R995 Self-transmissible broad-host range IncP vector [28]
R995-VC6 A derivative of plasmid R995 with a cloned 1,209 bp DNA fragment of T6SSSPI-6 from S. Dublin This study
R995 + SPI-6 T6SSSPI-6 cluster from S. Dublin cloned in vector R995 This study
R995 + SPI-19 T6SSSPI-19 cluster from S. Gallinarum 287/91 cloned in vector R995 [25]
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Veterinary Research

ISSN: 1297-9716