Table 1 Primers used in the RT-qPCR assays of sorted porcine NK cells
From: Porcine CD8αdim/-NKp46high NK cells are in a highly activated state
Target gene – accession number | Target | Primer sequences | Position on + strand | Product length (bp) | Product melt.temp (°C) |
|---|---|---|---|---|---|
forward (F) and reverse (R) | |||||
NM_001123143 | NKp46 | primers as published in [20], rtprimerdb ID: 8346 | 110 | 87.0 | |
EU282355.1 | NKp30 | F: TCTATTACCAGGGCAAATGTGAAGT | 345 | 209 | 91.0 |
R: GTCACTGGGGTCTAGAATCACTCAT | 554 | ||||
NM_213813.1 | NKG2D | F: ACAGCAGAGAAGACCAGGATTTCTTCA | 598 | 104 | 82.0 |
R: GGAACCATCTTCCCACTGCCAGG | 702 | ||||
XM_003135179.3 | CXCR3 | F: CCGACCACAAGCACCAAAGCA | −69 | 94 | 90.0 |
R: TGGCGTTGGCTCATCTCAGGGA | 25 |