Colostral antibody induced interference of inactivated bluetongue serotype-8 vaccines in calves
© Vitour et al; licensee BioMed Central Ltd. 2011
Received: 8 April 2010
Accepted: 28 September 2010
Published: 2 February 2011
Since its introduction into northern Europe in 2006, bluetongue has become a major threat to animal health. While the efficacy of commercial vaccines has been clearly demonstrated in livestock, little is known regarding the effect of maternal immunity on vaccinal efficacy. Here, we have investigated the duration and amplitude of colostral antibody-induced immunity in calves born to dams vaccinated against bluetongue virus serotype 8 (BTV-8) and the extent of colostral antibody-induced interference of vaccination in these calves. Twenty-two calf-cow pairs were included in this survey. The median age at which calves became seronegative for BTV was 84 and 112 days as assayed by seroneutralisation test (SNT) and VP7 BTV competitive ELISA (cELISA), respectively. At the mean age of 118 days, 13/22 calves were immunized with inactivated BTV-8 vaccine. In most calves vaccination elicited a weak immune response, with seroconversion in only 3/13 calves. The amplitude of the humoral response to vaccination was inversely proportional to the maternal antibody level prior to vaccination. Thus, the lack of response was attributed to the persistence of virus-specific colostral antibodies that interfered with the induction of the immune response. These data suggest that the recommended age for vaccination of calves born to vaccinated dams needs to be adjusted in order to optimize vaccinal efficacy.
Bluetongue (BT) is a non-contagious, arthropod-borne viral disease affecting sheep, some species of wild ruminants such as deer, and to a lesser extent, cattle and goats. BT is a major concern in the international trade of animals and animal products. Bluetongue virus (BTV), the etiologic agent of the disease, is the type species of the genus Orbivirus within the family Reoviridae. It is transmitted almost exclusively by the bite of Culicoides midges. Since 1998, five distinct serotypes of BTV (1, 2, 4, 9 and 16) have spread across southern and central Europe [1, 2]. In August 2006, a sixth serotype, BTV-8, was first identified in northern Europe, from where it quickly spread throughout the Netherlands, Belgium, Luxembourg, Germany and northern France [3–6]. The virus overwintered successfully in the same regions and then spread to the United Kingdom, Denmark, Switzerland and the Czech Republic in 2007. The BTV-8 strain circulating in northern Europe exhibits several unusual properties, and notably its ability to cause disease and mortality in cattle [2, 7]. Mass vaccination campaigns were quickly instituted to limit the spread and the dramatic socioeconomic consequences of the BT outbreak in Europe. Commercially available inactivated vaccines are now widely used to control BTV infection, and since 2008 vaccination of cattle is compulsory in some endemic European countries. An issue that was not sufficiently addressed prior to approval of inactivated BTV vaccines was the optimum age at which calves born to vaccinated dams should be vaccinated so as to avoid colostral antibody-induced interference.
Newborn calves acquire passive immunity from their dams by ingestion and absorption of antibodies present in colostrum. The estimated duration and benefit of this passively derived humoral immunity can vary greatly depending on the colostrum production (quantity and quality) and the quantity of antibody ingested and absorbed. Maternally derived immunity can confer protection against a broad range of viral pathogens including bovine herpesvirus-1 (BHV-1), bovine viral diarrhea virus (BVDV) and bovine respiratory syncytial virus (BRSV) [8–12]. Passive immunity frequently blocks the production of serum antibodies when immunogens are administered to calves with maternally derived antibodies , even if in some cases immunogens can induce immunological memory that is not susceptible to maternal antibody regulation [14, 15]. Also, vaccination against BHV-1 and BRSV with modified live virus (MLV) vaccines can generate immune responses such as lymphocyte blastogenesis in calves with maternal antibodies to BHV-1 and BRSV . However, very few data are available as regards the duration and effect of maternally acquired immunity against BTV in calves that were born to vaccinated cows. This prompted us to investigate: (1) the time required for nursing beef calves to become seronegative; (2) the effect of colostral antibodies on the humoral response in calves after vaccination with an inactivated BTV-8 vaccine.
Materials and methods
Twenty-two pairs of calves-pregnant cattle originating from two distinct farms located in the north of France (Tour and Font) were included in the survey. All cows were vaccinated in May 2008 with an inactivated BTV-8 vaccine (Bovilis BTV8; Intervet) which was administered subcutaneously according to the manufacturer's instructions. The vaccinated cows were all seropositive for BTV immediately prior to calving, as determined by a VP7-specific BTV competition ELISA (cELISA) test (data not shown). The cattle presented no clinical symptoms and no BTV RNA was detected by real-time PCR (BTVM-Kit TAQVET™; LSI, France; data not shown). The calves were born in October 2008 under standard management conditions. Blood samples were collected from calves at five time points; the first sample (S1) was collected at approximately 48 days post-calving (range 36-60), S2 at 80 days (range 70-90), S3 at 111 days (range 102-122), S4 at 139 days (range 127-150) and S5 at 202 days (range 189-207). Calves were tested for the presence of BTV antibodies by cELISA and serum neutralisation test (SNT). At a mean age of 118 days, 13/22 calves (3 from Tour and 10 from Font) were vaccinated with the Bovilis BTV-8 inactivated vaccine. The vaccine was administered as 2 subcutaneous injections 3 weeks apart.
BTV antibody levels were measured using the cELISA ID Screen® Bluetongue Competition assay (ID VET, Montpellier, France) according to the manufacturer's instructions. Results are expressed as percentage of negativity (PN) compared with the kit control and designated as positive, doubtful or negative according to the cut-off values recommended by the manufacturer (PN ≤ 35 is positive; 35 < PN ≤ 45 is doubtful; PN > 45 is negative). Statistical analyses were performed using a threshold value of 35 to discriminate between positive (PN ≤ 35) and negative (PN > 35) BTV cELISA results (see below).
Serum Neutralisation Test (SNT)
SNT was performed using the BTV-8 South African reference strain and serotype-specific BTV-8 positive control antisera. Briefly, 50 μL of sera were diluted (1:2 to 1:256) and titrated against 100 TCID50 (50 μL) of the BTV-8 South African reference virus. Plates were incubated for 1 h at 37°C and then 100 μL (2 × 104) of a Vero (African green monkey kidney) cell suspension was added per well. After incubation at 37°C for 5-7 days, the wells were scored for cytopathic effect (CPE). The neutralisation titre was defined as the dilution of serum resulting in 50% neutralisation endpoint. Titres were expressed as the log10 of the reciprocal endpoint serum dilution. The positive-negative cut-off was 0.9 (titre < 0.9: negative; titre ≥ 0.9: positive).
The interval after birth when calves suckling seropositive cows became seronegative was determined using the non-parametric survival Kaplan-Meier method. Survival analysis was used to address data describing how long it takes for an event to occur. In this study, the event is the fact that an animal becomes seronegative. This event was not observed in some calves that were still seropositive prior to vaccination: these were treated as censored observations. Independent survival analyses were conducted using the cELISA and SNT results.
A Kappa value was computed to determine the agreement between cELISA and SNT in sera from non-vaccinated animals (sera from vaccinated animals collected before the vaccination date was also included). Kappa values were interpreted according to Dohoo et al. .
The success of BTV vaccination was defined on the basis of cELISA performed on samples collected three months after the first vaccination. Analysis of variance (ANOVA) was used to assess the existence of a relationship between vaccination success and cELISA response at S1, S2 and S3. A receiver operating characteristic (ROC) analysis  was then conducted to model the probability of vaccination success according to the cELISA response at S3. The cELISA cut-off resulting in optimal predictions of vaccination success was determined. In order to establish the relation between age at vaccination and vaccination success, we used the preceding cELISA cut-off to study the time interval until cELISA rises above this value (corresponding to a negative status relatively to this cut-off). The Kaplan-Meier survival analysis method was used to compute the age at which vaccination would succeed in 50% of calves.
All statistical analysis and graphs were performed with R .
Correlation between SNT and ELISA
BTV antibody detection in serum samples from non-vaccinated calves using cELISA and SNT.
Estimated time to seronegative status using survival analysis
Antibody response to BTV vaccination
cELISA PN antibody levels in vaccinated animals before vaccination and seroconversion status.
S1 (48 d)
S2 (80 d)
S3 (111 d)
S4 (139 d)
S5 (202 d)
Neg or Dbt (10/13)
cELISA level and vaccination success
After being shown to be inversely related to the humoral response elicited by vaccination, cELISA titres immediately prior to vaccination (S3) were used for ROC analysis. A sensitivity and specificity of 100% were obtained with cELISA cut-off values between 76 and 103.
Following the 2006 outbreak of BTV-8 in livestock in northern Europe, BT has become a major concern in animal health and trade. Vaccine manufacturers rapidly provided efficacious and safe inactivated BTV-8 vaccines that are now widely and successfully used throughout Europe to control BTV infection [20, 21]. However, extensive studies have not been performed to evaluate the effects of passive immunity in calves born to immunized dams on the humoral response to BTV-8 immunization. In this survey, we followed the kinetics of loss of BTV-8 specific colostral antibodies in calves born to vaccinated dams using SNT and cELISA assays. Comparison of the antibody levels observed in these two tests showed only a moderate agreement, likely reflecting the fact that cELISA and SNT measure distinct antibody populations: the SNT titre declined more quickly than the cELISA titre, and the kappa value was lower than the overall kappa at the second sampling date (S2) while it improved at the third sampling date (S3) when the ELISA values had increased.
Recently, Oura et al. found that 22/22 lambs fed with maternal colostrum from ewes vaccinated with the Intervet Bovilis BTV-8 inactivated vaccine had protective neutralising antibodies at 10 weeks of age and 14/22 (64%) at 14 weeks of age . The persistence of neutralising antibodies appears slightly longer than in our study (84 days by SNT), possibly due to species differences or study design. Following challenge with BTV-8 at 13 to 14 weeks of age, all lambs were protected from clinical disease and only 5/22 showed transitory BTV RNA. These data show that maternally acquired antibodies directed against BTV can confer long-lasting protection against BTV infection. However, in another study, the duration of protection may well have been shorter, as no neutralising antibodies were found in calves born from BTV-2 vaccinated dams after 39 days of age . It should be noted that several parameters could affect the duration of colostral antibodies, including the type of vaccine, the quantity of antibodies consumed and absorbed from the colostrum, and the rate of decay.
To determine the optimal age for vaccination of calves, it is crucial to know at what time colostral antibodies will have sufficiently waned so as not to interfere with the vaccine. Currently, the recommended age for vaccination of calves with inactivated BTV-8 vaccines varies from 1 to 3 months, depending on the vaccine manufacturer. However, these recommendations have not been based on extensive study, especially on the impact of colostral antibodies on the vaccinal response. Several studies have shown that colostrum-fed or vaccinated animals with neutralising antibodies are generally protected against infection, but that not all animals without neutralising antibodies are fully susceptible [21, 22, 24]. In the present study, vaccination of colostrum-fed calves with an inactivated viral vaccine poorly induce anti-BTV humoral immunity in most cases (Table 2 and Figure 3). From the data, it appears that antibody levels measured by cELISA are more predictive of vaccinal responsiveness than SNT. Indeed, a few days before vaccination, all strong responders to vaccination displayed cELISA-based PN ≥ 103, while the group of non-responders exhibited mixed positive and negative values, but all with PN < 76. Using SNT, both responders and non-responders showed BTV antibody titres below the detection limit, precluding distinction between the two groups. Moreover, by combining the survival analysis of the BTV colostral antibodies and the responsiveness to vaccination, we could estimate the time required to achieve a vaccination success rate of 50% as falling between 148 and 180 days of age, depending on the cELISA cut-off used; the maximum vaccination success probability would be reached at around 7 months. Due to the small number of animals involved in this survey, these results need to be confirmed and refined in a larger cohort. However, this predictive value confirms the apparent long-lasting presence of a protective humoral immunity against BTV in calves.
It is of critical importance to assure a shift from maternal to vaccination protection without any gap in protection. Even though maternally-derived immunity may block the antibody response in calves, anti-viral vaccination at the earliest possible time may be desirable, as many calves acquire insufficient levels of maternal antibodies . Such calves should therefore respond to vaccination, which raises the issue of the difficulty in designing and implementing a universally successful vaccination program. Since it is not conceivable to measure colostral antibodies before immunization, in order to address the high levels of interference produced from colostral antibodies, it may be wise to propose that: (i) during periods of vector activity (May-October) and potential virus circulation, calves born to vaccinated dams should be vaccinated on two occasions, before three months and then once again around the age of six months in order to ensure maximal protection and provide vaccinal coverage for cases of poor colostrum intake or inefficient vaccination of the dams; (ii) outside these periods, in the absence of BTV circulation, it seems unnecessary to vaccinate before weaning as maternally-derived immunity prevents the humoral response to vaccination, and vaccination around 5-6 months should be adequate. Further investigations on this issue could help to address the revision of current recommendations.
The authors are grateful to farmers and their veterinarians from Font and Tour livestock who kindly provided animals and blood samples for this survey.
- Mellor PS, Wittmann EJ: Bluetongue virus in the Mediterranean Basin 1998-2001. Vet J. 2002, 164 (1): 20-37. 10.1053/tvjl.2002.0713.View ArticlePubMedGoogle Scholar
- Schwartz-Cornil I, Mertens PP, Contreras V, Hemati B, Pascale F, Breard E, Mellor PS, MacLachlan NJ, Zientara S: Bluetongue virus: virology, pathogenesis and immunity. Vet Res. 2008, 39 (5): 46-10.1051/vetres:2008023.View ArticlePubMedGoogle Scholar
- Elbers AR, Backx A, Meroc E, Gerbier G, Staubach C, Hendrickx G, van der Spek A, Mintiens K: Field observations during the bluetongue serotype 8 epidemic in 2006. I. Detection of first outbreaks and clinical signs in sheep and cattle in Belgium, France and the Netherlands. Prev Vet Med. 2008, 87 (1-2): 21-30. 10.1016/j.prevetmed.2008.06.004.View ArticlePubMedGoogle Scholar
- Saegerman C, Berkvens D, Mellor PS: Bluetongue epidemiology in the European Union. Emerg Infect Dis. 2008, 14 (4): 539-544. 10.3201/eid1404.071441.PubMed CentralView ArticlePubMedGoogle Scholar
- Darpel KE, Batten CA, Veronesi E, Shaw AE, Anthony S, Bachanek-Bankowska K, Kgosana L, bin-Tarif A, Carpenter S, Muller-Doblies UU, Takamatsu HH, Mellor PS, Mertens PP, Oura CA: Clinical signs and pathology shown by British sheep and cattle infected with bluetongue virus serotype 8 derived from the 2006 outbreak in northern Europe. Vet Rec. 2007, 161 (8): 253-261. 10.1136/vr.161.8.253.View ArticlePubMedGoogle Scholar
- Toussaint JF, Vandenbussche F, Mast J, De Meester L, Goris N, Van Dessel W, Vanopdenbosche E, Kerkhofs P, De Clercq K, Zientara S, Sailleau C, Czaplicki G, Depoorter G, Dochy JM: Bluetongue in northern Europe. Vet Rec. 2006, 159 (10): 327-10.1136/vr.159.10.327-a.View ArticleGoogle Scholar
- Elbers AR, Backx A, Ekker HM, van der Spek AN, van Rijn PA: Performance of clinical signs to detect bluetongue virus serotype 8 outbreaks in cattle and sheep during the 2006-epidemic in The Netherlands. Vet Microbiol. 2008, 129 (1-2): 156-162. 10.1016/j.vetmic.2007.10.034.View ArticlePubMedGoogle Scholar
- Belknap EB, Baker JC, Patterson JS, Walker RD, Haines DM, Clark EG: The role of passive immunity in bovine respiratory syncytial virus-infected calves. J Infect Dis. 1991, 163 (3): 470-476.View ArticlePubMedGoogle Scholar
- Bolin SR, Ridpath JF: Assessment of protection from systemic infection or disease afforded by low to intermediate titers of passively acquired neutralizing antibody against bovine viral diarrhea virus in calves. Am J Vet Res. 1995, 56 (6): 755-759.PubMedGoogle Scholar
- Cortese VS, West KH, Hassard LE, Carman S, Ellis JA: Clinical and immunologic responses of vaccinated and unvaccinated calves to infection with a virulent type-II isolate of bovine viral diarrhea virus. J Am Vet Med Assoc. 1998, 213 (9): 1312-1319.PubMedGoogle Scholar
- Howard CJ, Clarke MC, Brownlie J: Protection against respiratory infection with bovine virus diarrhoea virus by passively acquired antibody. Vet Microbiol. 1989, 19 (3): 195-203. 10.1016/0378-1135(89)90066-7.View ArticlePubMedGoogle Scholar
- Mechor GD, Rousseaux CG, Radostits OM, Babiuk LA, Petrie L: Protection of newborn calves against fatal multisystemic infectious bovine rhinotracheitis by feeding colostrum from vaccinated cows. Can J Vet Res. 1987, 51 (4): 452-459.PubMed CentralPubMedGoogle Scholar
- Husband AJ, Lascelles AK: Antibody responses to neonatal immunisation in calves. Res Vet Sci. 1975, 18 (2): 201-207.PubMedGoogle Scholar
- Brar JS, Johnson DW, Muscoplat CC, Shope RE, Meiske JC: Maternal immunity to infectious bovine rhinotracheitis and bovine viral diarrhea viruses: duration and effect on vaccination in young calves. Am J Vet Res. 1978, 39 (2): 241-244.PubMedGoogle Scholar
- Menanteau-Horta AM, Ames TR, Johnson DW, Meiske JC: Effect of maternal antibody upon vaccination with infectious bovine rhinotracheitis and bovine virus diarrhea vaccines. Can J Comp Med. 1985, 49 (1): 10-14.PubMed CentralPubMedGoogle Scholar
- Ellis JA, Hassard LE, Cortese VS, Morley PS: Effects of perinatal vaccination on humoral and cellular immune responses in cows and young calves. J Am Vet Med Assoc. 1996, 208 (3): 393-400.PubMedGoogle Scholar
- Dohoo I, Martin W, Stryhn H: Veterinary epidemiologic research. 2003, Charlottetown, Prince Edward island, Canada: AVC IncGoogle Scholar
- Greiner M, Pfeiffer D, Smith RD: Principles and practical application of the receiver-operating characteristic analysis for diagnostic tests. Prev Vet Med. 2000, 45 (1-2): 23-41. 10.1016/S0167-5877(00)00115-X.View ArticlePubMedGoogle Scholar
- R development Core Team: R: A Language and Environment for Statistical Computing. 2009, Vienna, Austria: R Foundation for Statistical ComputingGoogle Scholar
- Eschbaumer M, Hoffmann B, Konig P, Teifke JP, Gethmann JM, Conraths FJ, Probst C, Mettenleiter TC, Beer M: Efficacy of three inactivated vaccines against bluetongue virus serotype 8 in sheep. Vaccine. 2009, 27 (31): 4169-4175. 10.1016/j.vaccine.2009.04.056.View ArticlePubMedGoogle Scholar
- Oura CA, Wood JL, Sanders AJ, Bin-Tarif A, Henstock M, Edwards L, Floyd T, Simmons H, Batten CA: Seroconversion, neutralising antibodies and protection in bluetongue serotype 8 vaccinated sheep. Vaccine. 2009, 27 (52): 7326-7330. 10.1016/j.vaccine.2009.09.070.View ArticlePubMedGoogle Scholar
- Oura CA, Wood JL, Floyd T, Sanders AJ, Bin-Tarif A, Henstock M, Edwards L, Simmons H, Batten CA: Colostral antibody protection and interference with immunity in lambs born from sheep vaccinated with an inactivated Bluetongue serotype 8 vaccine. Vaccine. 2010, 28 (15): 2749-2753. 10.1016/j.vaccine.2010.01.028.View ArticlePubMedGoogle Scholar
- Savini G, Monaco F, Calistri P, Panichi G, Ruiu A, Leone A, Caporale V: Neutralising antibody response in cattle after vaccination with monovalent modified-live vaccine against bluetongue virus serotype 2. Vet Ital. 2004, 40 (4): 668-670.PubMedGoogle Scholar
- Savini G, MacLachlan NJ, Sanchez-Vizcaino JM, Zientara S: Vaccines against bluetongue in Europe. Comp Immunol Microbiol Infect Dis. 2008, 31 (2-3): 101-120. 10.1016/j.cimid.2007.07.006.View ArticlePubMedGoogle Scholar
- McGuire TC, Pfeiffer NE, Weikel JM, Bartsch RC: Failure of colostral immunoglobulin transfer in calves dying from infectious disease. J Am Vet Med Assoc. 1976, 169 (7): 713-718.PubMedGoogle Scholar
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