Figure 5

DMXAA or HKCA training activates mTOR signaling. WT PM were trained with DMXAA or HKCA for 24 h and rested for 5 days. A The cellular lysate was analyzed for p-mTOR, p-AKT, HIF1α, p-TBK1, p-IRF3, and β-actin by Western blotting. B–F The gray intensity value of proteins was calculated using ImageJ software. Data are shown as mean ± SEM. Data were pooled from 3 independent experiments. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.