Figure 2

Trained immunity activates STING signaling. WT PM were trained with HKCA and then were restimulated with LPS or C. perfringens at an MOI of 5 for the indicated times. A, D Whole cell lysates were analyzed for STING, p-TBK1, p-IRF3, and β-actin by Western blotting. B, C, E, and F The gray intensity value of proteins was calculated using ImageJ software. G IFN-β mRNA levels were measured at 4 h post‑infection by qRT‑PCR. H CXCL10 mRNA levels were measured at 24 h post‑infection by qRT‑PCR. Data are shown as mean ± SEM. Data were pooled from 3 independent experiments. Statistical significance is indicated by *p < 0.05, **p < 0.01, and ***p < 0.001.