Figure 4

Efficiency of gene editing in PK1-C5. A Transfection and lentiviral transduction efficiency in PK1-C5 cells. Scale bar = 200 μm. B Immunostaining for ASFV p30 and p72 protein in ASFV infected PK1-C5 cells and p72 gene targeting sgRNA transduced (PK1-C5-P72^KO) cells. Scale bar = 200 μm. C Western blot for detection of ASFV p30, p54 and p72 proteins in ASFV infected PK1-C5 cells and potential PK1-C5-P72^KO cells. D Virus titers in PK1-C5 cells and PK1-C5-P72^KO cells at 36 hpi.