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Figure 2 | Veterinary Research

Figure 2

From: A porcine kidney-derived clonal cell line with clear genetic annotation is highly susceptible to African swine fever virus

Figure 2

Infection and replication of ASFV in PK1-C5. A Visualization of infection with ASFV field strains in PK1-C5 cells and PAMs by immunostaining with an antibody against viral p30 protein (green). Scale bar = 200 μm B PK1-C5 cells and PAMs were infected with P1, P5, P10 and P15 viruses at MOI = 1, while IFA was utilized to detect ASFV proliferation in PK1-C5 cells through consecutive virus passages. Scale bar = 200 μm. C Growth curve of ASFV in PK1-C5 cells and PAMs. D In vitro growth kinetics of passaged ASFV (P1, P5, P10, P15) in PAMs. E In vitro growth kinetics of passaged ASFV (P1, P5, P10, P15) in PK1-C5 cells.

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