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Figure 4 | Veterinary Research

Figure 4

From: Swine acute diarrhea syndrome coronavirus Nsp1 suppresses IFN-λ1 production by degrading IRF1 via ubiquitin–proteasome pathway

Figure 4

Inhibition of the RLR signaling pathway by SADS-CoV nsp1. A Expression of MAVS, TBK1, IKKε, and IRF1 in IPI-FX cells detected by Western blot. B–E SADS-CoV nsp1 inhibits the RLR signaling pathway. IPI-FX cells were co-transfected with nsp1, along with expression plasmids for MAVS (B), TBK1 (C), IKKε (D), or IRF1 (E), as well as IFN-λ1-luc and pRL-TK at a ratio of 1:1:0.1:0.01 for 24 h. Cell lysates were prepared, and luciferase activity was measured. Data are represented as the mean ± SD of three replicates. ***P < 0.001.

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Veterinary Research

ISSN: 1297-9716