Figure 1

SADS-CoV efficiently infects IPI-FX cells. A\ Expression of SADS-CoV N protein in infected-cells detected by immunofluorescence assay. IPI-FX cells were either mock-infected or infected with SADS-CoV at different multiplicities of infection (MOI = 0.5, 1, 2). At 24 and 36 hpi, cells were fixed and incubated with a polyclonal antibody against SADS-CoV N protein (green). Scale bar = 100 μm. B Expression of SADS-CoV N mRNA in infected-cells detected by RT-qPCR. IPI-FX cells were either mock-infected or infected with SADS-CoV at a MOI of 1. The mRNA level of the SADS-CoV N gene was measured at the indicated hours post-infection using RT-qPCR. C Expression of SADS-CoV N protein in infected cells detected by Western blot. IPI-FX cells were either mock-infected or infected with SADS-CoV at a MOI of 1. Cell extracts were prepared at 12, 24, and 36 hpi and subjected to Western blot analysis. D Induction of IFN-λ in IPI-FX cells after poly(I:C) stimulation. Cells were transfected with or without 1 μg/mL poly(I:C). At 12 hpi, the mRNA levels of the IFN-λ1, IFN-λ3, and IFN-λ4 genes were measured using RT-qPCR. The mRNA levels of IFN-λ after poly(I:C) stimulation were normalized to those of individual IFN-λ without treatment. Data are represented as the mean ± SD of three replicates. **P < 0.01; ***P < 0.001.