Figure 1
Pasteurella multocida infection destroys pulmonary epithelial integrity and induces lethal bacteremia. A Photographs of murine lungs infected with or without 104 CFU of PmCQ2. B Representative images of H&E staining and Masson’s trichrome staining of murine lungs infected with 104 CFU of PmCQ2. Scale bars = 200 μm. C Survival curves of mice infected with 104 CFU of PmCQ2. D The bacterial load of murine lungs infected with 104 CFU of PmCQ2. N = 6. The data are presented as the means ± SDs. E The bacterial load of murine extrapulmonary tissues (liver, kidney, and spleen) intranasally infected with 104 CFU of PmCQ2. N = 6. The data are presented as the means ± SDs. F A schematic of the Evans blue treatment protocol. G Representative photographs of murine lungs after Evans blue treatment. H Quantification of Evans blue in the lungs and serum obtained from PmCQ2-infected mice and control mice. N = 5. The data are presented as the means ± SDs. I Blood bacterial load of PmCQ2-infected mice at different time points. N = 5. The data are presented as the means ± SDs. J Survival curves of control group mice, intranasal infection group mice, intravenous infection group mice and half intranasal half intravenous infection group mice. All infection groups were infected with a total of 104 CFU PmCQ2, while the control group mice received saline. K Survival curves of control group mice, intranasal infection group mice, intravenous infection group mice and half intranasal half intravenous infection group mice. All infection groups were infected with a total of 108 CFU of PmCQ6, while the control group mice received saline. Every single point represents one individual. ** p < 0.01, *** p < 0.001.