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Figure 6 | Veterinary Research

Figure 6

From: Interleukin-2 enhancer binding factor 2 negatively regulates the replication of duck hepatitis A virus type 1 by disrupting the RNA-dependent RNA polymerase activity of 3D polymerase

Figure 6

Suppression of the expression of ILF2 by the replication of DHAV-1 in vitro. A The expression levels of ILF2 in DEFs at 4, 6, and 12 hpi were measured through Western blot analysis using anti-ILF2 antiserum (1:100). B The grayscale value of each protein band was measured using ImageJ software. The y-axis represents the ratio of the ILF2 grayscale value to that of GAPDH, the x-axis represents the time post-infection (hours). C The qRT-PCR results show the expression level of ILF2 at 4, 6, and 12 hpi in DEFs. D Western blot analysis of ILF2 expression in DEH at 4, 6, and 12 hpi. The anti-ILF2 antiserum (1:100) was used. E The grayscale value of ILF2 in the DEH at different times post-infection. F The qRT-PCR results showed the expression level of ILF2 at 4, 6, and 12 hpi in DEH. Bars show the mean ± SD of three independent experiments (n = 3).

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Veterinary Research

ISSN: 1297-9716