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Figure 8 | Veterinary Research

Figure 8

From: The spontaneously produced lysogenic prophage phi456 promotes bacterial resistance to adverse environments and enhances the colonization ability of avian pathogenic Escherichia coli strain DE456

Figure 8

Prophage phi456 genes influence survival ability in macrophage cells HD-11. A The bacterial survival in HD-11 infected with DE456 or DE456Δphi456 at a MOI of 20 bacteria per cell. At 3 h or 6 hpi, the cells were lysed and bacterial counts were measured. B Quantification of genes related to toll-like receptors and tumor necrosis factor by qRT-PCR. The quantification of the target gene was performed by normalizing to the reference gene β-Actin. C Quantification of genes related to oxidative stress response by qRT-PCR. The relative expression level of rpoS, katE and oxyR in the DE456Δphi456 was compared to WT. The quantification of the target gene was performed by normalizing to the reference gene dnaE. The error bars indicate standard deviations. Student’s t-test was used to analyze the data.

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Veterinary Research

ISSN: 1297-9716